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Journal : HAYATI Journal of Biosciences

The Effect of Enhancing Ulvan's Antioxidant Properties in Supplemented Diets on Accelerating The Phenoloxidase Immune Response in White Shrimp Azhar, Nuril; Yudiati, Ervia; Ambariyanto; Trianto, Agus
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1116-1129

Abstract

This study investigated the total phenol content (TPC) and DPPH inhibition of ulvan from Ulva lactuca, along with its supplementation effects on the specific growth rate (SGR) and phenoloxidase (PO) activity of Litopenaeus vannamei. Ulva lactuca samples were processed to obtain four ulvan extracts using different methods such as P-HWE (Polysaccharide-Hot Water Extract), O-HWE (Oligosaccharide-Hot Water Extract), P-A-HWE (Polysaccharide-Acid-Hot Water Extract), and O-A-HWE (Oligosaccharide-Acid-Hot Water Extract). The U. lactuca powder was treated with 80% ethanol overnight, then centrifuged and dried. Depigmented U. lactuca was extracted with water (65°C, 2 hours), and polysaccharides were precipitated with 99% ethanol, yielding P-HWE. P-HWE was heated at 145°C for 4.5 hours to yield O-HWE. Another extraction after depigmentation, using water containing H2O2 and ascorbic acid (65°C, 2 hours), precipitated P-A-HWE, which was dried and heated to yield O-A-HWE. FTIR analysis provided insights into the chemical composition and structural characteristics of ulvan extracts. TPC and DPPH inhibition were measured spectrophotometrically, and PO activity using a colorimetric assay with L-DOPA. Ulvan supplementation in shrimp feed was evaluated for growth and immune response. Results showed significant differences in TPC, DPPH inhibition, and PO activity among extracts, with O-A-HWE having the highest phenol content and DPPH inhibition. Ulvan supplementation significantly influenced the SGR of L. vannamei, with the highest SGR in the O-A-HWE treatment. This study suggests that ulvan extracts, especially O-A-HWE, could be effective natural immunostimulants for enhancing the health and growth of L. vannamei, warranting further research to optimize extraction methods and understand the underlying mechanisms.
Antibacterial, Antifungal, Antioxidant, and Photoprotective Analysis of Mangrove Extracts as Additives Ingredients in a Cosmetic Cream Putri, Dear Berliana; Pringgenies, Delianis; Trianto, Agus
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.571-580

Abstract

Mangroves are a rich source of natural antioxidant compounds that can inhibit and reduce tissue damage caused by free radical activity. Currently, cosmetic creams are widely recommended for maintaining skin health and aesthetics, especially in protecting against the harmful effects of free radicals. This study applied mangrove extract as an additive ingredient in a cosmetic cream, serving as an antibacterial, antifungal, antioxidant, and photoprotective agent. Mangrove samples were collected from the mangrove ecosystem in Tapak Village, Semarang, Indonesia. Three mangrove species were evaluated for their bioactivity, and the species with the highest bioactivity was selected for use in the cream formulation. Antibacterial and antifungal activities were tested using the disc diffusion method, antioxidant activity was assessed using the DPPH method, and photoprotective activity was determined using UV spectrophotometry, with analysis based on the Mansur mathematical equation. The cream was evaluated for its characteristics, including spreadability, homogeneity, sensory properties, pH, sun protection factor (SPF), phytochemical content, stability, and microbial contamination. An in vivo was conducted to assess the cream’s effectiveness on white mice. Results indicated that Avicennia marina leaf extract exhibited the highest bioactivity compared to the other two species, Rhizophora mucronata and Bruguiera gymnorrhiza. Consequently, A. marina leaf extract was selected as the active additive ingredient in the cream formulation. Characterization tests demonstrated that the cream was stable and met standard criteria for quality. The in vivo analysis revealed that the mangrove leaf extract cream significantly prevented epidermal thinning, reduced neutrophil counts, and preserved fibroblast numbers.
Co-Authors Agus Sabdono Akhmad Tri Prasetyo Aldi Nuary Nuary Alghazeer, Rabia Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Ridlo Amanda, Leony Chika Ambariyanto Ambariyanto , Ambariyanto Ambariyanto Andi Afriandi Andi Afriandi Andika, Reta Anggeraeni, Dwi Anicia Hurtado Aninditia Sabdaningsih Apriliani, Seka Indah ardiansyah, vendra Ariesta, Ria Arono Arono Ayu Charismawaty Ayu, Trifajriah Lutea Azhar, Nuril Azis Rifai Aziz Rifai Bahry, Muhammad Syaifudien Bayu Utomo Bimo Saskiaoktavian Carlos, Juan Catur Wulandari Christel Marty Dafa, Yori Hayyan Delianis Pringgenies Desi Anggraini Destio . Desy Wulan Triningsih DIAH AYU SATYARI UTAMI Diah Permata Wijayanti Diah Permata Wijayanti Diana, Cris Dwi Haryanti Dwi Haryo Ismunarti Dwi Lestari Widya Ningsih Dwi Susilaningsih Eko Nurcahya Dewi Elfanando Rizky Juliantara Sucipto Elis Indrayanti Endang Supriyantini Endra Wijaya Ervia Yudiati Farrastasya Muflihul Azzami Fatra, Qalbita Fran Ciputra Tambunan Frans Alexander Nainggolan Gita Wismayanti Gumono Gumono, Gumono Hafni Rahmawati Hamzah, Syukri Haqqu, Alir Adn Humairah Arifia Sabiladiyni Irene Ulsadriatny Irwani Irwani Isai Yusidharta Ita Puspitasari Ita Widowati Jumarta, Rofi Jusup Supriyanto Jusup Supriyanto Karim, Murni Kristi, Roselina Nadya Larasati, Stefanie Jessica Henny Lestari, Martini Tria Lilik Maslukah M. Arifin Ma'ruf, Widodo Farid Mada Triandala Sibero Mada Triandala Sibero Maestro Munru Mahadika Fanindhita Sany Maulani, Febianti Aisyah McCauley, Erin Muhammad Arifin Muhammad S. Bahry Muhammad Syaifudien Bahry Muhammad Syaifudien Bahry Munasik Munasik Munasik Munru, Maestro Nafri Yanti Nathalie Bourgougnon Ni Komang Tri Utami Nining Nursalim Nugraha Ridho Ikhsani, Nugraha Ridho Nugroho Agus D Nugroho Hendartono Nugroho Hendartono Nur Taufiq-Spj Nuskiya, Amila Ocky Karna Radjasa Popi IL Ayer Purwadi, Agus Joko Puspitasari, Ita Putri, Dear Berliana Rachmat Afriyanto Raden Ario Radisya N Nissa Rahmaini, Putri Raja Aditia Sahala Siagian Retno Hartati Retno Murwani Rexie Magdugo Rizky Rifatma Jezzi Rohimat Rohimat Rosida, Nina Rosita Dewi, Anggi Rudhi Pribadi Rudhi Pribadi Sabiladiyni, Humairah Arifia Sakti Imam Muchlisin Sari, Dian Lupita Sari, Else Puspita Sarjito - Sarwono, Sarwit Sekar Widyaningsih Sembiring, Yudhawira Bhaskara Septhy Kusuma Radjasa Shari, Vio Deswitha Shofiatun Nimah Sri Sedjati Subagiyo Subagiyo Sucipto, Elfanando Rizky Juliantara Susetyo Susetyo Tambunan, Fran Ciputra Taufiq-Spj, Nur Tri Winarni Agustini Trifajriah Lutea Ayu Twenty Agustiola, Agnes Ujiana, Rica Utomo, Padi Widianingsih Widianingsih Wiratno Wiratno Wisman Wismayanti, Gita Wittriansyah, Khoeruddin Yonnawati, Yonnawati Yudhawira Bhaskara Sembiring Yulistio, Didi