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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Buletin Peternakan Jurnal Peternakan Sriwijaya Jurnal Veteriner Jurnal Ilmu dan Industri Peternakan JURNAL PETERNAKAN Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Peternakan Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Zeolit Indonesia Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Animal Production : Indonesian Journal of Animal Production Jurnal Ternak Wahana Peternakan Livestock and Animal Research Anoa: Journal of Animal Husbandry Jurnal Kedokteran Hewan Journal of Animal Research and Applied Science Jurnal Agripet
Niken Ulupi
Animal Production And Technology, Faculty Of Animal Science, Bogor Agricultural University, Bogor, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Journal : Jurnal Ilmu Ternak Veteriner

 1 
Novel Mutation of Exon 5 Prolactin Gene in IPB-D1 Chicken Lailatul Rohmah; Sri Darwati; Niken Ulupi; Isyana Khaerunnisa; Cece Sumantri
Jurnal Ilmu Ternak dan Veteriner Vol 25, No 4 (2020): December 2020
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v25i4.2525

Abstract

The prolactin gene (PRL) is a gene that controls the incubation and egg production in laying chickens. The nature of incubation will reduce egg production and disrupt the reproductive system in local chickens. The purpose of this study was to identify the polymorphism of prolactin genes in IPB-D1 chickens using the direct sequencing method. The polymorphism of the exon 5 prolactin gene was carried out on 46 samples of IPB-D1 chicken DNA which was a collection of the Division of Animal Genetics and Breeding, Faculty of Animal Science IPB. DNA sequences as a reference for designing exon 5 primers were obtained from the National Center for Biotechnology Information (NCBI) with the GenBank access code: AF288765.2. DNA extraction was carried out using the phenol-chloroform technique. DNA amplification resulted in a PCR product with a size of 557 bp. In this study, the genotype frequency, allele frequency, heterozygosity value and Hardy-Weinberg equilibrium were calculated. The results of the study found 5 SNPs in exon 5, namely g.7823A>G, g.7835A>G, g.7886T>A, g.8052T>C, and g.8069T>C. All SNPs are polymorphic and in Hardy-Weinberg equilibrium except g.8052T>C. The g.7823A>G, g.7835A>G, g.8052T>C SNPs are synonymous mutations that do not change amino acids, while the g.7886T>A and g.8069T>C SNPs are non-synonymous that change amino acids. Both g.7886T>A and g.8069T>C SNPs are potential as a marker assisted selection for the characteristics of egg production in IPB-D1 chickens.
Polymorphisms of the Thy1 (Thymocyte Differentiation Antigen 1) Gene in Candidate IPB D2 Chicken Line Nensy Tri Putri; Sri Murtini; Niken Ulupi; Isyana Khaerunnisa; Cece Sumantri
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 4 (2022): December 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i4.3029

Abstract

The Thy1 gene is a member of the immunoglobulin superfamily, which is found in many different animals. This gene encodes a Thy1 protein (CD90) which measures 25–37 kDa. Although the function of the Thy1 gene is unknown, it is thought to play a crucial role in the immunological response, particularly in the activation of T cells. The goal of this study was to find a variation in the Thy1 gene in IPB D2  chickens that might be utilized as candidates for immunoresistance genetic markers. A total of 123 samples from IPB D2 chickens consisting of 38 samples from IPB D2 G0 (basic generation) chickens and 85 samples from IPB D2 G2 (second generation) chickens were used in this study. PCR methods and direct-DNA sequencing were used in this study. Data analysis in this study consisted of genotype frequency, allele frequency, observed heterozigosity, expected heterozygosity, and Hardy-Weinberg equilibrium. The results of the study  discovered two SNPs on the intron (g.2139 CT and g.2542 AG). Four haplotypes were created by combining two SNPs. The g.2139 CTand g.2542 AG were polymorphic, but not in the Hardy-Weinberg equilibrium.
Production Performance and Sperm Characteristics of Quail (Coturnix-coturnix japonica) with Different Concentrations of Yolk Immunoglobulin Satri Yusasra Agasi; Niken Ulupi; Rudi Afnan; R. Iis Arifiantini
Jurnal Ilmu Ternak dan Veteriner Vol 28, No 2 (2023): June 2023
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v28i2.3173

Abstract

Quails are classified according to their immunity to different IgY concentrations. Quail IgY concentrations range from 0 to 1200 µg ml-1, and these IgY concentrations may affect production and reproductive performance. This study aimed to analyze IgY concentrations in male quail and to compare production and reproductive performance at different IgY concentrations. Forty-two male quail at five weeks of age participated in the study, including 29 quail with low IgY concentration (210-393 µg ml-1)   and, 13 with intermediate IgY concentration (403-564 µg ml-1). The observed productive performances were feed intake, initial body weight, final body weight, weight gain, feed conversion, morbidity, and mortality. In addition, this study observed testicular weight and macroscopic and microscopic semen quality for reproductive traits. Differences in production and reproductive performance of each group were analyzed using an independent-sample t-test. The result showed that male quail with different IgY concentrations were equal in all productive traits (P0.05). This means that male quail with low IgY and medium IgY concentrations are equally good. Testicular weight, semen color and pH were identical at different IgY concentrations. Quails with low IgY concentrations have better sperm consistency, which is related to the weight of the testes, which are heavier in quails with low IgY concentrations than in those with moderate IgY concentrations. This consistency is related to the concentration of sperm in the semen. The result concludes IgY concentrations in male quail did not affect production performance. Quails with low IgY concentration had thicker sperm consistency and higher sperm concentration.
Co-Authors A.S. Satyaningtijas Abdul Alim Yamin Agasi, Satri Yusasra Agpretasia, Yodilla Ahmad Furqon Ainul Khadija Saleema Alfiansyah, Teuku Muhammad Alifiya, Qorina Apriadi Pasaribu Arief, Irma Isnafia Aryani Satyaningtijas Asep Gunawan Asma'ul Fitriana Nurhidayah Asmaul Fitriana Nurhidayah Asmaul Fitriana Nurhidayah C Sumantri C Sumantri Cece Sumantri Damhuri Daryatmo Daryatmo David Anwar Dawanto, Jirfan Dede Kardaya Desrayni Hanadhita Dewi Kartika Dhini Nova Widyasari Dwi Nurhidayah Zubaidah Edi Erwan Elok Budi Retnani Erwan, Edi Fitra, Deni Fredi Kurniawan Guslian Abdul Basir Harahap, Ahmad Saleh Hariono Hariono Hariono Hariono Hasrin Hayu Fitriyani HERA MAHESHWARI I wayan Teguh Wibawan Ida Ayu Putu Sri Widnyani Insan Mujahid Afnan Irma Isnafia Arief Irwan Oktoni Isyana Khaerunnisa Jakaria Jakaria Jefri Naldi Jonathan Anugrah Lase Jumadin, La Kaiin, Ekayanti Mulyawati Khusnul Khatimah koekoeh santoso Komariah (Komariah) La Jumadin Lailatul Rohmah Lili Darlian Luki Abdullah Mala Nurilmala Maryce Agusthinus Walukou Mauludi, Muhammad Rifqi Melly Pratiwi Setyawati Melly Pratiwi Setyawati Muhamad Najibulloh Muhammad Amrullah Pagala Muhsinin, Muhammad Nahrowi Nahrowi Nambut, Yohanes Baptisto Nensy Tri Putri Priyambodo, Danang R. Iis Arifiantini Ria Ariyanti Rita Mutia Rudi Afnan Sadarman Sadarman Salundik Silaban, Yosua Kristianto Siti Aminah Sri Darwati sri murtini . Sri Purwanti St Chadijah St. Chadijah Tarigan, Indri Nur Krisna Teguh Rafian TIKE SARTIKA Tuti Suryati Wahyuni Wahyuni Wahyuni Wahyuni Yani Fatriana Yulvian Sani Yusman Setiawan Zakiah Wulandari