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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Buletin Peternakan Jurnal Peternakan Sriwijaya Jurnal Veteriner Jurnal Ilmu dan Industri Peternakan Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) JURNAL PETERNAKAN Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Peternakan Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Zeolit Indonesia Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Animal Production : Indonesian Journal of Animal Production Jurnal Ternak Wahana Peternakan Livestock and Animal Research Anoa: Journal of Animal Husbandry Jurnal Kedokteran Hewan Journal of Animal Research and Applied Science Jurnal Agripet

Niken Ulupi

Animal Production And Technology, Faculty Of Animal Science, Bogor Agricultural University, Bogor, 16680, Indonesia

Author-ID : 407573

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Identifikasi Keragaman Gen Kalpastatin (CAST) pada Ayam Lokal Indonesia (IDENTIFICATION OF POLYMORPHISM CALPASTATINE GENE IN LOCAL CHICKEN) Ahmad Saleh Harahap; Cece Sumantri; Niken Ulupi; Sri Darwati; Tike Sartika
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Calpastatin (CAST) gene is one of the genes that play a role in the process of tenderization of meat. CAST gene serves as an inhibitor of meat tenderness. The purpose of this study was to identify Single Nucleotide Polymorphisms (SNP) in the CAST gene of kampong chickens. A total of 61 kampong chickens used were used in the study including strain cobb, F1 of strain cobb-kampong, merawang, sentul, nunukan, and pelung, respectively. The method used is extracted DNA from blood samples, then amplified by Polymerase Chain Reaction (PCR) and then genotyping by DNA sequencing. The results showed that SNP was identified at position g.42988G> T in the intron 11 CAST gene of merawang chicken with genotype GG and GT. SNP was not identified in kampong chicken, strain cobb, F1 of strain cobb-kampong, sentul, nunukan, and pelung chicken, respectively. In conclusion, the intron 11 CAST gene region with 482 of product length of merawang chicken is polymorphic, whereas the other chicken species is monomorphic. ABSTRAK Gen Calpastatin (CAST) merupakan salah satu gen yang berperan dalam proses keempukan pada daging. Gen CAST berfungsi sebagai inhibitor (penghambat) keempukan daging. Tujuan dari penelitian ini adalah mengidentifikasi Single Nucleotide Polymorphisms (SNP) gen CAST pada ayam kampung. Jumlah ayam yang digunakan 61 ekor ayam kampung, ayam strain cobb, F1 ayam strain cobb-kampung, ayam merawang, ayam sentul, ayam nunukan, dan ayam pelung. Metode yang dilakukan adalah ekstrasi DNA dari sampel darah, kemudian dilakukan amplifikasi menggunakan mesin Polymerase Chain Reaction (PCR) dan selanjutnya dilakukan genotiping melalui DNA sequencing. Hasil penelitian menunjukkan bahwa gen CAST intron 11 pada ayam pengamatan ditemukan SNP posisi g.42988G>T pada ayam merawang dengan genotip GG dan GT, sedangkan pada ayam kampung, ayam strain cobb, F1 ayam kampung dengan strain cobb, ayam sentul, ayam nunukan, ayam merawang dan ayam pelung tidak ditemukan SNP. Simpulan dari penelitian ini memperlihatkan gen CAST daerah intron 11 dengan panjang produk 482 pada ayam merawang bersifat polimorfik, sedangkan jenis ayam yang lain bersifat monomorfik.

A Polymorphism of Insulin-Like Growth Factor Binding Protein 2 Gene Associated with Growth and Body Composition Traits in Kampong Chickens Ahmad Furqon; Asep Gunawan; Niken Ulupi; Tuti Suryati; Cece Sumantri
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Insuline-like growth factor binding protein 2 (IGFBP2) is one of the principal binding proteins that has biological functions involved in growth, development, and differentiation. Selection for rapid growth based on molecular Marker Assisted Selection is required to increase production performance. The present study was designed to analyze the associations of IGFBP2 gene polymorphisms with chicken growth and body composition traits. Kampong chicken, a native chicken in Indonesia, is slow-growing chicken. A total of 59 males were used in the current study. Growth and body composition were measured in 24 wk of age. Primers for intron 2 region were designed from genomic chicken sequence. A c.1032C>T SNP of the IGFBP2 gene intron 2 region was detected and PCR-RFLP method was then used to genotype Kampong chicken population. The result showed that IGFBP2 polymorphism was significantly associated with body, carcass, breast, breast muscle, pectoralis minor, leg, and wings weight in Kampong chicken population (P < 0.05). The research suggests that the IGFBP2 gene could be a candidate gene that affects growth and body composition traits in chicken.

Identifikasi Keragaman Gen Toll-Like Receptor-4 Ayam Lokal dengan Polymerase Chain Reaction- Restriction Fragment Lenght Polymorphism (THE GENETIC POLIMORPHISM OF TOLL-LIKE RECEPTOR-4 GENE IN LOCAL CHICKENS USING POLYMERASE CHAIN REACTION-RESTRICTION FRAG Niken Ulupi; Muladno .; Cece Sumantri; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Toll-like Receptor 4 (TLR4) gene is one of the genes that control resistance of chickens againstSalmonella sp. through non-specific immune response. These gene can be used as genetic markers inIndonesian local chickens, if known its diversity. Therefore this research was aimed at evaluating thegenetic polymorphism of TLR4 gene in several types of Indonesian lokal chickens (kampung, kampungpelungcrossbreed, sentul, and tolaki chicken) using PCR-RFLP. The total of samples were 136. There werethree stages of identification : extraction of DNA genom, PCR amplification of TLR4 gene (with size 220 bpon exon 2), and RFLP method using restriction enzym (MscI). The data were analyzed include frequency ofallele, frequency of genotype, heterozygosity values , and value of Polymorphic Informative Content (PIC).The result showed that TLR4|MscI was found polymorphic in all kind of chickens that was genotyped. Itwere found two alleles (A and G), and three genotypes (AA, AG, and GG). The value of x2 showed thatTLR4|MscI was in Hardy-Weinberg equilibrium. The value of Ho and He were 0,11-0,41 and 0,11-0,35. Thevalue of PIC (0,10-0,29) included in the category of low to moderately high. These results declared thatTLR4|MscI gave hope for used as genetic markers in resistance to Salmonella sp. infection in Indonesianlocal chickens.

Co-Authors A.S. Satyaningtijas Abdul Alim Yamin Agasi, Satri Yusasra Agpretasia, Yodilla Ahmad Furqon Ainul Khadija Saleema Alfiansyah, Teuku Muhammad Alifiya, Qorina Apriadi Pasaribu Arief, Irma Isnafia Aryani Satyaningtijas Asep Gunawan Asma'ul Fitriana Nurhidayah Asmaul Fitriana Nurhidayah Asmaul Fitriana Nurhidayah C Sumantri C Sumantri Cece Sumantri Damhuri Damhuri Daryatmo Daryatmo David Anwar Dawanto, Jirfan Dede Kardaya Desrayni Hanadhita Dewi Kartika Dhini Nova Widyasari Dwi Nurhidayah Zubaidah Edi Erwan Elok Budi Retnani Erwan, Edi Fitra, Deni Fredi Kurniawan Guslian Abdul Basir Harahap, Ahmad Saleh Hariono Hariono Hariono Hariono Hasrin Hayu Fitriyani HERA MAHESHWARI I wayan Teguh Wibawan Ida Ayu Putu Sri Widnyani Insan Mujahid Afnan Irma Isnafia Arief Irwan Oktoni Isyana Khaerunnisa Jakaria Jakaria Jefri Naldi Jonathan Anugrah Lase Jumadin, La Kaiin, Ekayanti Mulyawati Khusnul Khatimah koekoeh santoso Komariah (Komariah) La Jumadin Lailatul Rohmah Lili Darlian Luki Abdullah Mala Nurilmala Maryce Agusthinus Walukou Mauludi, Muhammad Rifqi Melly Pratiwi Setyawati Melly Pratiwi Setyawati Muhamad Najibulloh Muhammad Amrullah Pagala Muhsinin, Muhammad Nahrowi Nahrowi Nambut, Yohanes Baptisto Nensy Tri Putri Nurhidayah, Asma'ul Fitriana Priyambodo, Danang R. Iis Arifiantini Ria Ariyanti Rita Mutia Rudi Afnan Sadarman Sadarman Salundik Salundik Silaban, Yosua Kristianto Siti Aminah Sri Darwati sri murtini . Sri Purwanti St Chadijah St. Chadijah Tarigan, Indri Nur Krisna Teguh Rafian TIKE SARTIKA Tuti Suryati Wahyuni Wahyuni Wahyuni Wahyuni Yani Fatriana Yulvian Sani Yusman Setiawan Zakiah Wulandari

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