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Journal : Cropsaver : Journal of Plant Protection

Assessment of Sarocladium oryzae Growth Inhibition, the Causal Agent of Rice Sheath Rot Disease, Using Methanol Extract of Binahong Leaves Endah Yulia; Silviya Rahma Dhiya Syafira; Fitri Widiantini; Wawan Kurniawan
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.6 KB) | DOI: 10.24198/cs.v2i1.22301

Abstract

Sheath rot disease caused by Sarocladium oryzae is an important disease on rice. The pathogen that infects uppermost leaf sheath enclosing to the young panicles can cause yield losses up to 85%. Though the disease is commonly controlled using synthetic fungicides, the use of natural products that safer to the environment have been enormously explored. The research aimed to test growth inhibition of S. oryzae colony using methanol leaf extract of binahong (Anredera cordifolia). The research was carried out from June until December 2017 at the Laboratory of Biotechnology Plant Protection, Department of Plant Pest and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The method used was Randomized Complete Design consisted of six treatments and four replications. The treatments were five concentrations of binahong leaf extract of 0.125%, 0.25%, 0.5%, 1%, 2% and one control treatment. The result showed that binahong leaf extract did not inhibit the growth of S. oryzae. The highest inhibition percentage of 4.55% at 0.25% concentration was considered to be a low inhibition category. Although the colony growth of S. oryzae was not significantly inhibited, the extract caused hyphal abnormalities. This actually shows the antifungal potency of the binahong leaf extract that needed further examination with more adequate methods.Keywords : Anredera cordifolia, botanical pesticide, rice sheath rot
Potency of Bacillus subtilis and Lysinibacillus Mixed With Silica Nano Particles and Carbon Fiber in Controlling Potato Leaf Blight Disease [Phytophthora infestans (Mont.) de Bary] Hersanti Wartono; Fitri Widiantini; Kirana Sonya Harviana
CROPSAVER Vol 2, No 2 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (406.821 KB) | DOI: 10.24198/cropsaver.v2i2.22473

Abstract

Leaf blight disease caused by Phytohthora infestans is one of the major diseases on potato plant. To control the disease farmers usually use synthetic fungicides. However, the excessive use of synthetic fungicides may cause negative impacts on the environment. One of the alternative control methods which is more environtmentally friendly is the use of antagonistic microbes. In this experiment, Bacillus subtilis and Lysinibacillus sp. were formulated with carbon fiber as a carrier and enriched with nano silica as a micro nutrient, to find out the ability of the bacteria in suppressing leaf blight disease on potato plant. The experiment was carried out in the experimental field in Lembang, Bandung Barat, Jawa Barat. The experiment was arranged in the randomized block design consisted of nine treatments and four replications. Density of B.subtilis and Lysinibacillus sp. were 1011 cfu/ml. The formulation was applied 3 times, on planting, 2 weeks and 4 weeks after planting. The results showed that the application of B. subtillis and Lysinibacillus sp. were able to suppress leaf blight disease, with the disease inhibition 36,6% and 32,7% respectively. The combination treatment of B. subtilis in silica nano and carbon fiber was able to suppress leaf blight disease on potato plant higher compared to the application of bacteria without nano silica and carbon fiber, with the disease inhibition up to 51,2%.
EndophyticBbacteria origin of healthy rice plants produce antifungal volatile compound inhibited the growth of Pyricularia oryzae Cav., the causal agent of rice blast disease Fitri Widiantini; Fuji Hartati
CROPSAVER Vol 3, No 1 (2020)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (632.908 KB) | DOI: 10.24198/cropsaver.v3i1.28121

Abstract

Endophytic bacteria have been known as producers for bioactive compound in which widely used in the field of crop protection. Several isolates of endophytic bacteria have demonstrated their potential ability as biocontrol agents by inhibiting the development of major rice pathogens. However, their ability to produce bioactive compounds has not been explored. The study reported the ability of the endophytic bacteria isolates in producing antifungal volatile compounds to inhibit the growth of Pyricularia oryzae Cav., the causal agent of rice blast disease. The experiment was conducted by dual culture method using bipartite compartments in which the bacterial isolates and P. oryzae were grown separately and placed by facing to each other. The bacterial isolates were grown on ISP2 agar media, whereas P. oryzae was grown on PDA media. Nine isolates were tested for their ability in producing antifungal volatile compound. The potential of antifungal volatile compound was observed by the P. oryzae growth compared to control. Furthermore, its effect on the P. oryzae mycelia was observed under microscope. The result demonstrated that all of the tested isolates were able to produce volatile compound which were able to inhibit the growth of P. oryzae. In addition, the compounds were also causing mycelial alteration.
Inhibition Effects of Culture Filtrates and Volatile Compounds of Antagonistic Microbes Isolated from Vermicompos and Compost Teas on the Growth of Alternaria solani Sor. in Vitro Noor Istifadah; Adelia Septiandini; Sri Hartati; Fitri Widiantini
CROPSAVER Vol 5, No 2 (2022)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v5i2.43278

Abstract

Alternaria solani Sor. is one of destructive pathogens in solanaceous plants including tomato. Bacteria and yeast isolated from water extract of organic matters are potential as biological control agents of plant pathogenic fungi. Mechanisms of antagonism of bacteria and yeast can be through antibiosis. This study was conducted to examine the abilities of culture filtrate and volatile compounds produced by antagonistic bacteria and yeast isolated from compost and vermicompost teas to inhibit the growth of A. solani in vitro. The experiments were arranged in randomized complete design with four replications. The culture filtrate experiment applied well diffusion method, while the volatile compound effect experiment used petri dish sandwich method. The results showed that the culture filtrates of four bacteria and three yeast isolates inhibited the growth of A. solani in vitro by 16.6-87.5%. The highest inhibition level was showed by KSB4 isolate (Bacillus subtilis), a bacterial isolate from cow manure compost tea. In the volatile compound effect experiment, the tested bacteria and yeast isolates inhibited the pathogen growth by 31.3-75.2%, with the highest inhibition was showed by KcB3, a bacterial isolate from vermicompost tea. The isolate that its culture filtrate and volatile compounds both showed high inhibition level (62.7% and 87.5%) on A. solani growth was KSB4 isolate (B. subtilis).
Inventory and Disease Incidence in 38 Accessions of Taro Plants (Colocasia esculenta L.) in Jatinangor, Sumedang Regency, West Java Yulia, Endah; Yunira, Alma; Hidayat, Syarif; Djaya, Luciana; Widiantini, Fitri; Suganda, Tarkus; Karuniawan, Agung
CROPSAVER Vol 7, No 2 (2024)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v7i2.58942

Abstract

Taro is an important agricultural commodity with considerable prospects for international market competition. However, its cultivation faces several challenges, particularly pathogen infections that can lead to substantial yield losses. Conducting a disease inventory in taro plants is essential for effective disease management and serves as a preliminary step in developing resistant taro varieties. This study aimed to document diseases affecting 38 accessions of taro plants. The research was conducted from August to October 2021 at the Ciparanje Jatinangor Experimental Field and the Phytopathology Laboratory within the Department of Plant Pests and Diseases at the Faculty of Agriculture, Universitas Padjadjaran, located in Sumedang Regency. The methodology employed both qualitative and quantitative descriptive approaches. Data collection involved observing the incidence and severity of diseases, as well as identifying the pathogens responsible for the diseases. A total of 292 taro plants were examined during this study. The diseases identified included brown leaf spot (Cladosporium colocasiae), shot hole (Phoma sp.), orange leaf spot (Neojohnstonia colocasiae), white leaf spot (putative Pseudocercospora colocasiae), and leaf blight (Phytophthora colocasiae), along with other symptoms suspected to be caused by root pathogens and viruses.The most prevalent disease observed at the experimental site was brown spot disease, while leaf blight was identified as the most damaging. The incidence of leaf spot and leaf blight reached 100% across nearly all accessions of taro planted at the research site, with the highest severity of disease recorded at 49.65%.
Co-Authors Adelia Septiandini Aditya Bintan Pratama Agung Karuniawan Agung Kurniawan Agus Susanto Agus Susanto Agus Susanto Aina Anna Roosda Andang Purnama Andang Purnama Andang Purnama Asri Peni Wulandari Avissa Ayuningdiyas Betty Natalie Fitriatin Ceppy Nasahi Citra Bakti, Citra Danar Dono Danar Dono Deni Ariyono Dewi, Vira Kusuma Dinda Sekar Ayu Fiko Dwindry Formanda Dwisari Januarily Pitaloka Elga Sari Ema Komalasari Ema Komalasari, Ema Emma Trinurani Sofyan Endah Yulia Endah Yulia Endah Yulia Endah Yulia Yulia Fajri Maulana Anugrah Fakhrurroja, Hanif Feliatra Fiko, Dinda Sekar Fitri, Elysa Fuji Hartati Fuji Hartati Ghifari, Raden Faqih Hilmiy Hadi Syahir Muhadam Hersanti - Hersanti HERSANTI Hilda Sandra Utami Ida Nurhelawati Ida Nurhelawati Ifa Dwi LUTHFIANA Kang, Seung Won Kartika Sari Kirana Sonya Harviana Krissanti, Petra Sulistya Dian Lindung Tri Puspasari Luciana Djaya, Luciana Margaretta Christita, Margaretta Mia Rahmah Qadryani Mitalo, Oscar W. Muhammad Maksum Nabilla, Anisa Neneng S Widayani Nia Rossiana Nono Carsono Nono Carsono Noor Istifadah Noor Istifadah Nugraha, Gema Takbir Parakkasi, Karmilla Prabowo, Yudhisa Hendri Putra, Fitriansyah Putu Ghita Novilaressa Ramdan Firmansah Rangga Irawan Prasetyo RIKA MELIANSYAH Riswandi, Hafiz Rizqullah, Ahmad Fauzan Safri Ishmayana Santa Monica Santika Sari Satriyo Restu Adhi Serly Noviyawati Silviya Rahma Dhiya Syafira Siska Rasiska SIska Rasiska, SIska Sri Hartati Sri Hartati Sudarjat Sudarjat Sukmono Suwignyo Susanti, Rista Syahnur, Fitika Syarif Hidayat Tarkus Suganda Tati Herlina Tiara Febricyanti Putri Senjaya Tualar Simarmata Vira Kusuma Dewi Vira Kusuma Dewi Wahyu Daradjat Natawigena, Wahyu Daradjat Wawan Kurniawan Yulia, Endah Yulia Yunira, Alma Yusup Hidayat Yusup Hidayat