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All Journal AQUATIC SCIENCE & MANAGEMENT JURNAL PESISIR DAN LAUT TROPIS JURNAL ILMIAH PLATAX Jurnal Bios Logos JURNAL PERIKANAN DAN KELAUTAN TROPIS BULETIN OSEANOGRAFI MARINA JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI Jurnal Ilmiah Sains Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Stenly Wullur
Fakultas Perikanan Dan Ilmu Kelautan Universitas Sam Ratulangi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Mathematics Medicine & Pharmacology Physics

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Isolation and Screening the Symbiont Bacteria of the Sponge Dragmacidon sp from Manado Bay, North Sulawesi that Producing Chitinase and Protease Sembiring, Sindiy Cloudya; Warouw, Veibe; Wullur, Stenly; Bara, Robert A; Salaki, Meiske S.; Ginting, Elvy Like
Jurnal Ilmiah Platax Vol. 9 No. 1 (2021): ISSUE JANUARY - JUNE 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.9.1.2021.34307

Abstract

Enzymes are important in the technology industry and hydrolytic enzymes, such as chitinase and protease are commonly used for it. Various types of microorganisms such as bacteria can produce hydrolytic enzymes.  Sponge-associated bacteria are excellent sources of extracellular hydrolytic enzymes because the surface and internal spaces of sponges are richer in nutrients. The aim of this study was to isolate and screen the bacteria of the sponge Dragmacidon sp symbiotic from Manado Bay, North Sulawesi that producing chitinase and protease   Symbiont bacteria were grown in Zobell 1226 E medium with a dilution of 10-4. Bacterial isolation was carried out based on the morphological characteristics of the colony. Chitinase and protease activity was carried out by growing each bacterial isolate in chitin and protein media at 36oC for 48 hours. Chitinase and protease activities were indicated by the formation of a clear zone around the bacterial colony, however, the clear zone for chitinase activity was observed after pouring the Lugol's solution. Based on this study, 8 isolates bacteria of the symbiotic spongy Dragmacidon sp from Manado Bay, North Sulawesi were isolated based on morphological characteristics. The colony of the bacteria is generally white with an irregular shape. Four isolates, namely 1, 2, 3, and 8 had chitinase activity with chitinolytic indexes were 1.7; 1.5; 1.4, and 1.3, respectively. Six isolates, namely 1, 2, 3, 4, 5, and 6 had protease activity with proteolytic indexes were 1.4; 1.8; 3.1; 1.3; 1.8; and 2.5, respectively.Keywords: Bacteria; Chitinolytic; Proteolytic; Symbiont; SpongeAbstrakEnzim menempati posisi penting dalam bidang teknologi dan industri. Enzim yang banyak digunakan dalam bidang industri adalah enzim hidrolase. Enzim dapat diisolasi dari berbagai jenis mikroorganisme seperti bakteri. Bakteri yang berasosiasi dengan spons merupakan sumber enzim hidrolitik ekstraseluler yang sangat baik karena permukaan dan ruang internal spons lebih kaya nutrisi. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas kitinase dan protease bakteri simbion spons Dragmacidon sp dari Teluk Manado. Bakteri simbion spons ditumbuhkan dalam media Zobell 1226 E pada pengenceran 10-4. Isolasi bakteri dilaksanakan berdasakan karakteristik morfologi. Aktivitas kitinase dan protease dilaksanakan dengan menumbuhkan setiap isolat bakteri dalam media kitin dan protein pada suhu 36oC selama 48 jam. Aktivitas kitinase dan protease ditandai dengan terbentuknya zona bening di sekitar koloni bakteri yang mana untuk kitinase diamati setelah diberi larutan lugol. Berdasarkan penelitian ini, 8 isolat bakeri simbion spon Dragmacidon sp dari Teluk Manado, Sulawesi Utara berhasil diisolasi berdasarkan karakteristik morfologi. Isolat bakteri umumnya berwarna putih dengan bentuk ireguller. Empat isolat yakni 1, 2, 3, dan 8 memiliki aktivitas kitinase dan enam isolat yakni 1, 2, 3, 4, 5, dan 6 yang memiliki aktivitas protease. Indeks kitinolitik dari masing-masing keempat isolat bakteri secara berturut turut adalah 1,7; 1,5; 1,4; dan 1,3 dengan kategori bernilai rendah dan indeks proteolitik adalah 1,4; 1,8; 3,1; 1,3; 1,8; dan 2,5 dengan kategori bernilai rendah sampai tinggi.Kata kunci: Bakteri; Kitinolitik; Proteolitik; Simbion; Spons
Molecular Identification And Conservation Status Of Sharks From The Fins Trade In Manado City North Sulawesi Mopay, Maratade; Wullur, Stenly -; Onibala, Hens -; Ginting, Elvy Like; Rumengan, Inneke F. M; Sondak, Calvyn F.A; Sumilat, Deiske A.
Jurnal Ilmiah Platax Vol. 9 No. 2 (2021): ISSUE JULY-DECEMBER 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v9i2.36016

Abstract

Sharks are a group of cartilaginous fish that are vulnerable to overfishing.  Genetics approaches play an important role in shark conservation. Shark fishing has become the main activity of fishermen in several areas, including in North Sulawesi. This research is focused on the molecular aspects and conservation status of shark species obtained from the shark fin trade in Manado, North Sulawesi. COI gene was amplified using Fish BCL5 (for) and HCO219 (rev) primers. Nucleotide sequences of each sample were aligned with the closest sequences in the GenBank database using the BLAST (Basic Local Alignment and Search Tool) method. The conservation status of the shark species is carried through the IUCN (International Union for the conservation of nature red list) Red list website. Molecular identification results showed that the shark’s fins from Manado had high similarity with Carcharhinus falciformis (HM1 and HM2) and Carcharhinus melanopterus (HM3). According to IUCN red list data, the C. falciformis and C. melanopterus were categorized as vulnerable to extinction (VU).Keywords:  Molekuler; COI; Shark; Manado and IUCN Red list . AbstrakHiu merupakan kelompok ikan bertulang rawan yang sangat rentan terhadap dampak penangkapan secara berlebihan.  Informasi terkait genetik hiu  yang semakin terancam populasinya sangat berperan penting dalam upaya konservasi hiu. Penangkapan hiu telah menjadi aktivitas utama nelayan di beberapa daerah, termasuk di Sulawesi Utara. Penelitian ini difokuskan pada aspek  molekuler dan penentuan status konservasi spesies hiu menggunakan sampel sirip yang di dapatkan  dari perdagangan sirip  hiu, di kota Manado.  Amplifikasi gen COI dilakukan dengan menggunakan primer Fish BCL5 (for) dan HCO219 (rev). Sekuens nukleotida masing-masing sampel disejajarkan dengan nukleotida terdekat yang ada dalam database genbank menggunakan metode BLAST (Basic Local Aligment and Search Tool)  Penentuan status konservasi dilakukan melalui penelusuran spesies rujukan di situs IUCN (International Union for the Conservation of Nature) Red list. Hasil identifikasi molekuler menunjukan bahwa sampel sirip hiu dari  kota Manado  memiliki kemiripan yang tinggi dengan spesies : Carcharhinus falciformis  (HM1 dan HM2) dan C. melanopterus (HM3).  Menurut data IUCN Red list, C. falciformis dan C. Melanopterus merupakan jenis hiu dalam status konservasi rentan punah (VU). Kata Kunci: Molekuler; COI; hiu; Manado dan IUCN Red list
Antibacterial Activity of Stylissa carteri Sponge Extract from Manado Bay, North Sulawesi Palungan, Irpan; Bara, Robert Antonius; Mangindaan, Remy Emile Petrus; Kemer, Kurniati; Wullur, Stenly; Rembet, Unstain N. W. J.
Jurnal Ilmiah Platax Vol. 10 No. 1 (2022): ISSUE JANUARY-JUNE 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i1.36020

Abstract

Marine sponges contain secondary metabolites with unique chemical structures and very interesting pharmacological activities, such as antibacterial, anticancer, antiviral and others to be developed as candidate drugs. The presence of bioactive metabolites in sessile nature such as sponges also reflects the ecological adaptation formed during a long evolutionary process as a defense mechanism of this organism with its environment in the form of resistance to predation, competition and infection factors against pathogenic bacteria, so this study aims to examine the antibacterial activity of extracts and fractions of Stylissa carteri sponge fractions as well as testing the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values. The method used in testing the antibacterial activity is the agar diffusion method (Disc Diffusion Kirby Bauer Method). The presence of antibacterial activity was indicated by the formation of a clear zone around the paper disc after incubation for 24 hours. The results showed that antibacterial activity of S. carteri sponge extract tested on Bacillus megaterium DSM32T bacteria revealed to be the strongest inhibition zone of 21 mm. Further testing on the extract fraction of S. carteri showed that the semipolar fractions showed strong activity against the B. megaterium while the polar fraction was categorized as moderate action, the non-polar fraction showed no activity against the bacteria. The determination of the MIC and MBC values was obtained at 500 ppm and 1000 ppm respectively.
Antibacterial Activity of Endophytic Bacteria of Seagrass Symbiont Enhalus acoroides from Tiwoho Waters, North Minahasa Purniasih, Ni Komang Pitri; Ginting, Elvy Like; Wullur, Stenly; Mangindaan, Remy E. P.; Rumampuk, Natalie D. C.; Pratasik, Silvester Benny
Jurnal Ilmiah Platax Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.42485

Abstract

Nutrient-poor marine bacteria are often found to form a living mechanism by associating with other marine organisms such as seagrass Enhalus acoroides. Seagrass serves as a habitat for marine biota and is known to produce bioactive compounds. Endophytic and epiphytic microbes that are symbiotic in seagrass have the ability to produce bioactive compounds similar to the bioactive compounds produced by their host. Therefore, this study aimed to obtain isolates of symbiont bacteria and to test their antibacterial activity.  The symbiotic bacteria isolates were tested for their antibacterial activity using gram-negative bacteria such as E. coli and S. typhi, and gram-positive bacteria such as S. aureus and S. mutans. Antibacterial activity test was carried out using the paper disc method. Observation of antibacterial activity was carried out for 3x24 hours by observing the growth of the resulting inhibition zone. The zone of inhibition was measured to determine the diameter and strength of the bioactive compounds produced by symbiotic bacteria.A total of six isolates of symbiotic bacteria were obtained from the seagrass Enhalus acoroides with varied morphological characteristics. The six isolates of symbiont bacteria showed antibacterial activity and could inhibit and kill pathogenic bacteria. Two of the six isolates of symbiotic bacteria that produced the largest diameter of the inhibition zone were isolates E (Ep1) 3,5 mm and F (En3) 4 mm in S. mutans test bacteria.Keywords: Antibacterial, Isolation, Enhalus acoroides, Symbionts, Tiwoho AbstrakBakteri laut yang miskin nutrisi banyak dijumpai membentuk mekanisme hidup dengan cara berasosiasi dengan organisme laut lainnya seperti lamun Enhalus acoroides. Lamun berfungsi sebagai habitat biota laut dan diketahui dapat menghasilkan senyawa bioaktif. Mikroba endofit dan epifit yang bersimbiosis pada lamun mempunyai kemampuan untuk memproduksi senyawa-senyawa bioaktif yang serupa dengan senyawa bioaktif yang diproduksi inangnya. Oleh karena itu, penelitian ini bertujuan untuk mendapatkan isolat bakteri simbion serta menguji aktivitas antibakterinya.  Isolat bakteri simbion diuji aktivitas antibakterinya menggunakan bakteri uji gram negatif seperti E. coli dan S. typhi, dan bakteri uji gram positif seperti S. aureus dan S. mutans. Uji aktivitas antibakteri dilakukan menggunakan metode kertas cakram. Pengamatan aktivitas antibakteri dilakukan selama 3x24 jam dengan mengamati pertumbuhan zona hambat yang dihasilkan. Zona hambat diukur untuk mengetahui diameter dan kekuatan senyawa bioaktif yang dihasilkan oleh bakteri simbion.  Sebanyak enam isolat bakteri simbion yang didapatkan dari lamun Enhalus acoroides dengan karakteristik morfologi yang bevariasi. Keenam isolat bakteri simbion menunjukkan adanya aktivitas antibakteri dan dapat menghambat serta membunuh bakteri pathogen. Dua dari enam isolat bakteri simbion menghasilkan diameter zona hambat terbesar adalah isolat E (Ep1) 3,5 mm dan F (En3) 4 mm pada pada bakteri uji S. mutans.Kata kunci: Antibakteri, Isolasi, Enhalus acoroides, Simbion, Tiwoho
Sustainable Development Strategy For Water Tourism Park Conservation Area In North Minahasa Regency Mokoginta, Junio Marzuki Pratama; Paruntu, Carolus Paulus; Angmalisang, Ping Astony; Rompas, Rizald Max; Wullur, Stenly; Rondonuwu, Arie B.
Jurnal Ilmiah Platax Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.47581

Abstract

Abstract The Government of Indonesia has targeted 32.5 million hectares of marine conservation areas or 10% of the total area of ​​Indonesian waters in 2030. The location of the Water Tourism Park Conservation Area of North Minahasa Regency has been reserved by the North Sulawesi Provincial Government through Governor Decree Number 407 of 2018 covering an area of ​​25,838.91 hectares. The purpose of this research is to formulate a strategy for the sustainable development of the Water Tourism Park Conservation Area in North Minahasa Regency. This research was conducted for 6 months starting from June - December 2022. The method used in this research is a descriptive research method with survey techniques, namely by collecting data through interviews with relevant stakeholders as respondents. The results of this study obtained 10 (ten) priority strategies for the sustainable development of Water Tourism Park Conservation Area in North Minahasa Regency which will be recommended to the local government, in this case the Marine and Fisheries Office of North Sulawesi Province and related stakeholders as suggestions and materials that can be proposed in the context of making public policy in the field of marine and fisheries. Key words: Conservation; North Minahasa; Sustainable Development; Strategy; Water Tourism Park Abstrak Pemerintah Indonesia telah menargetkan 32,5 juta hektar kawasan konservasi perairan atau 10% dari luas perairan Indonesia pada tahun 2030. Lokasi Kawasan Konservasi Taman Wisata Perairan Kabupaten Minahasa Utara telah dicadangkan oleh Pemerintah Provinsi Sulawesi Utara melalui Surat Keputusan Gubernur Nomor 407 Tahun 2018 seluas 25.838,91 hektar. Tujuan penelitian ini untuk merumuskan strategi pengembangan berkelanjutan Kawasan Konservasi Taman Wisata Perairan di Kabupaten Minahasa Utara. Penelitian ini dilakukan selama 6 bulan dimulai dari Juni - Desember 2022. Metode yang digunakan dalam penelitian ini adalah metode penelitian deskriptif dengan teknik survey, yaitu dengan cara pengumpulan data melalui wawancara dengan para stakeholders terkait sebagai responden. Hasil dari penelitian ini didapatkan 10 (sepuluh) strategi prioritas untuk pengembangan berkelanjutan Kawasan Konservasi Taman Wisata Perairan di Kabupaten Minahasa Utara yang akan direkomendasikan kepada pemerintah daerah dalam hal ini Dinas Kelautan dan Perikanan Daerah Provinsi Sulawesi Utara beserta stakeholders terkait sebagai saran dan bahan yang dapat dipertimbangkan dalam rangka pembuatan kebijakan publik pada bidang kelautan dan perikanan. Kata kunci: Konservasi; Minahasa Utara; Pengembangan Berkelanjutan; Strategi; Taman Wisata Perairan
Molecular Phylogenetic Position of Bacillus sp Bacteria Isolated from Rotifer Culture Medium Based on Fisheries Waste Diet Wullur, Stenly
Jurnal Ilmiah Platax Vol. 11 No. 1 (2023): ISSUE JANUARY-JUNE 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i1.47975

Abstract

This study aims to identify and determine the phylogenetic position of two bacterial isolates (F0031 and F0033), isolated from culture media of Rotifer Brachionus plicatilis sp. complex. Genomic DNA of the bacteria was extracted using Qiaprep Miniprep Kit and the 16S rRNA gene was amplified using primer pairs 8F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-ACCTTGTTACGACTT-3'). The sequences were analyzed using Geneious Prime ver. 2020 and bacterial identification was performed using the Basic Local Alignment Tool (BLAST) method.  Phylogenetic analysis was conducted using MEGA X, where the phylogenetic tree was constructed using the Maximum Likelihood method, and evaluated using the Bootstrap method.  Results of this study showed that both bacterial isolates (F0031 and F0033) belong to different genera, namely Bacillus (F0031) and Mesobacillus (F0033). Bacterial isolate F0031 had the closest similarity and was located at the same phylogenetic branch as B. cereus, while isolate F0033 was with M. jeotgali. Keywords: phylogeny, molecular, bacteria, bacillus, mesobaccilus, rotifera, diet. Abstrak Penelitian ini bertujuan untuk mengidentifikasi dan menentukan posisi filogeni dua isolat bakteri (F0031 dan F0033) yang diisolasi dari media kultur rotifer Brachionus plicatili sp. complex.  DNA genom dari isolat bakteri diekstrak menggunakan Kit Qiaprep Miniprep dan gen 16S rRNA diamplifikasi menggunakan pasangan primer 8F (5'-AGAGTTTGATCCTGGCTCAG-3') dan 1492R (5'-ACCTTGTTACGACTT-3').  Hasil sekuens dianalisis menggunakan Sequence scanner ver. 2.0 dan analisis sekuens dilakukan menggunakan Geneious Prime ver. 2020.  Identifikasi isolat bakteri dilakukan dengan metode BLAST (Basic Local Alignment Tools) dan analisis filogenetik dilakukan dengan menggunakan MEGA X, dimana konstruksi filogeni menggunakan metode Maximum Likelihood yang dievaluasi dengan metode Bootstrap. Hasil penelitian menunjukan bahwa kedua isolat bakteri (F0031 dan F0033) merupakan isolat bakteri yang berasal dari genus berbeda, yaitu genus Bacillus (F0031) dan genus Mesobacillus (F0033).  Isolat bakteri F0031 memiliki kesamaan terdekat dan berada pada posisi percabagan filogenetik yang sama dengan B. cereus sedangkan Isolat F0033 dengan M. jeotgali Kata kunci: Filogeni, molekuler, bakteri, bacillus, mesobaccilus, rotifer, pakan
Mangrove Community Structure in West Madapolo Village, North Obi District, South Halmahera Regency Ode Mantra, Syahrun; Schaduw, Joshian N. W.; Sondak, Calvyn F.A.; Ompi, Medy; Manembu, Indri S.; Wullur, Stenly
Jurnal Ilmiah Platax Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.49712

Abstract

The ecological function of mangroves can be viewed from three aspects, namely physical, chemical, and biological aspects. Data collection of mangrove community structure using line transect quadrant method by pulling the line along 100 m from the sea to land at the lowest tide. The results of mangrove community structure analysis in West Madapolo Village, North Obi District, South Halmahera Regency. Identified 4 types of mangroves namely Rhizopora apiculata, Sonneratia alba, Brugueira gymnorhiza, and Lumnitzera litorea found at the 3 stations. The highest species density value is Rhizopora apiculata, at station 1. The highest frequency by Rhizopora apiculata, and Brugueira gymnorhiza species at station 1 with the same value of 39.29. The highest cover by Rhizopora apiculata species at station 1 with a value of 49.73 and the lowest by S. alba species at 14.17 while the highest importance value index by Rhizopora apiculata species at 147.99 and the lowest is Sonneratia alba 40.54. The highest diversity at station 2, and the lowest at station 1, the highest diversity at station 2 while the highest dominance is found at the station and the lowest at station 2. Keywords: Structure Community, Mangrove, Village Madapolo Barat Abstrak Fungsi ekologis mangrove dapat ditinjau dari tiga aspek, yakni aspek fisika, kimia dan biologi. Pengambilan data struktur komunitas mangrove menggunakan metode line transek kuadran dengan cara menarik line garis sepanjang 100 m dari arah laut ke darat pada saat surut terendah. Hasil analislis struktur komunitas mangrove di Desa Madapolo Barat, Kecamatan Obi Utara, Kabupaten Halmahera Selatan. Teridentifikasi 4 jenis mangrove yaitu Rhizopora apiculata, Sonneratia alba, Brugueira gymnorhiza, dan Lumnitzera litorea yang ditemukan pada ke 3 stasiun. Nilai kerapatan jenis tertinggi adalah Rhizopora apiculata, pada stasiun 1. Frekuensi tertinggi oleh jenis Rhizopora apiculata, dan Brugueira gymnorhiza pada stasiun 1 dengan nilai yang sama yaitu 39,29. Penutupan tertinggi oleh jenis Rhizopora apiculata pada stasiun 1 dengan nilai 49,73 dan yang terendah oleh jenis S. alba  14,17 sedangkan indeks nilai penting tertinggi oleh jenis Rhizopora apiculata 147,99 dan terendah yaitu Sonneratia alba 40,54. Keanekaragaman tertinggi pada stasiun 2, dan terendah pada stasiun 1, kseseragaman tertinggi pada stasiun 2 sedangkan dominasi tertinggi terdapat pada stasiun dan yang terendah pada stasiun 2. Kata kunci: Struktur Komunitas, Mangrove, Desa Madapolo Barat
Analysis Of Particle Content In Kappaphyccus alvarezii Seaweed In The Waters Of Arakan Village Using Scan Electron Microscopy–Energy Dispersive X-ray Spectroscopy (SEM-EDX) Tilaar, Sandra; Wullur, Stenly; Angkouw, Esther
Jurnal Ilmiah Platax Vol. 12 No. 1 (2024): ISSUE JANUARY-JUNE 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v12i1.51565

Abstract

Kappaphycus alvarezii seaweed is widely cultivated in Indonesia and has important economic value. Nevertheless, this seaweed is easily attacked by diseases. This study was to determine the ice-ice triggers in seaweed farming locations. This research study was conducted at Arakan Village, South Minahasa, North Sulawesi. We performed the bacteria isolation on the infected thallus of Kappaphycus alvarezii and found Staphylococcus arlettae caused the infection. Staphylococcus aureus used to be found on the skin and mucous membranes of healthy humans, while  Staphylococcus epidermis inhabits only the skin of healthy humans. Approximately 30% of the normal healthy population was affected by Staphylococcus aureus as it asymptomatically colonizes human hosts. How this Staphylococcus arlettae (mainly found in pork farms) infected Kappaphycus alvarezii which is in a high salinity environment requires an in-depth study. We completed the experiment by using Scan Electron Microscopy–Energy Dispersive X-ray Spectroscopy (SEM-EDX), analysis on infected thallus Kappaphycus alvarezii, and a series of bacteria isolation to observe the morphology and the element contents of Staphylococcus arlettae bacteria has successfully attached to the surface of Kappaphycus alvarezii and caused an infection.  This proves that environmental changes have stimulated pathogenic bacteria Staphylococcus arlettae in the area where Kappaphycus alvarezii is cultivated. The mechanism of biosorption by microbes that can live in environments that are contaminated with Pb metal is active uptake. This mechanism occurs simultaneously in line with the consumption of metal ions for the growth of microorganisms. Staphylococcus arlettae are resistant to heavy metals due to the ability to detoxify the influence of heavy metals in the presence of protein or granular material. From the results of the study using SEM-EDX of algae and bacteria Staphylococcus arlettae, it can be concluded that the content of the particle of metal in thallus triggers bacteria to live on the seaweed as an energy source. Keywords: Bacteria, disease, infection, Kappaphycus alvarezii, seaweed, Staphylococcus arlettae
Phytoplankton Community Structure in Seagrass Beds of Nain Island Waters Mokosuli, Febrianty Dhea; Rimper, Joice R.T.S.L; Warouw, Veibe; Wullur, Stenly; Losung, Fitje; Mokolensang, Jeffrie F.
Jurnal Ilmiah Platax Vol. 12 No. 1 (2024): ISSUE JANUARY-JUNE 2024
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v12i1.52339

Abstract

This study aims to identify phytoplankton species, calculate phytoplankton abundance, diversity index, uniformity index, phytoplankton dominance index, and determine environmental conditions such as temperature, pH, salinity, nitrate, and phosphate in the seagrass ecosystem of Nain Island Waters. Sampling includes seawater for the needs of phytoplankton identification measurement of nitrate and phosphate levels and measurement of water parameters in situ. Phytoplankton sampling is done horizontally using a plankton net. Phytoplankton identification found in the seagrass ecosystem of Nain Island Waters consisted of four classes, namely Bacillariophyceae (Rhizosolenia sp.; Nitzschia sp.; Chaetoceros sp.; Navicula sp.; Eucampi sp.; Pleurosigma sp.; Thalassionema sp.; Melosira sp.), Dinophyceae (Ceratium sp.; Protoperidinium sp.), Chlorophyceae (Spirogyra sp.), and Euglenophyceae (Euglena sp.). The calculation of phytoplankton abundance is in the range of 9 - 51 cells/l. The highest abundance was found at station one which was 51 cells/l, then station three which was 21 cells/l, and station two which was 9 cells//I. The diversity index is in the range of 0.8749 - 1.8668 which means there is community instability. The uniformity index is in the range of 0.2226 - 0.8460, while the dominance index is in the range of 0.2060 - 0.6093, this indicates that the water conditions are stable. Environmental conditions (temperature, salinity, pH, nitrate, and phosphate) in the seagrass ecosystem of Nain Island Waters are still quite good for phytoplankton growth. Keywords: Phytoplankton, Seagrass, Nain Island, Abundance, Biological Index Abstrak Penelitian ini bertujuan untuk identifikasi jenis-jenis fitoplankton, menghitung kelimpahan fitoplankton, indeks keanekaragaman, indeks keseragaman, indeks dominasi fitoplankton dan mengetahui kondisi lingkungan seperti suhu, pH, salinitas, nitrat, dan fosfat di ekosistem padang lamun Perairan Pulau Nain. Pengambilan sampel meliputi air laut untuk kebutuhan identifikasi fitoplankton dan pengukuran kadar nitrat dan fosfat perairan serta pengukuran parameter perairan secara In situ. Pengambilan sampel fitoplankton dilakukan secara horizontal dengan menggunakan plankton net. Fitoplankton yang ditemukan terdiri dari empat kelas yaitu Bacillariophyceae (Rhizosolenia sp. Nitzschia sp. Chaetoceros sp. Navicula sp. Eucampi sp. Pleurosigma sp. Thalassionema sp. Thalassionema sp. Melosira sp.), Dinophyceae (Ceratium sp. Protoperidinium sp.), Chlorophyceae (Spirogyra sp), dan Euglenophyceae (Euglena sp). Hasil perhitungan kelimpahan fitoplankton berada di kisaran 9 - 51 sel/l. Kelimpahan tertinggi ditemukan pada stasiun satu yaitu 51 sel/l, kemudian stasiun tiga yaitu 21 sel/l dan stasiun dua yaitu 9 sel/I. Indeks keanekaragaman berada pada kisaran 0,8749 - 1,8668 yang berarti adanya ketidakstabilan komunitas. Indeks keseragaman yaitu pada kisaran 0,2226 - 0,8460, sedangkan indeks dominasi berada pada kisaran 0,2060 - 0,6093, hal ini menunjukkan kondisi perairan dalam keadaan stabil. Kondisi lingkungan (suhu, salinitas, pH, nitrat dan fosfat) di ekosistem padang lamun Perairan Pulau Nain masih cukup baik untuk pertumbuhan fitoplankton. Kata kunci: Fitoplankton, Padang Lamun, Pulau Nain, Kelimpahan, Indeks Biologi
Isolation and Potential of Plastic-Degrading Bacteria from Plastic Waste Fatti, Clara; Rumampuk, Natalie Detty C.; Gerung, Grevo Soleman; Wullur, Stenly; Mamuaja, Jane Marianne; Ginting, Elvy Like
Jurnal Ilmiah Platax Vol. 13 No. 1 (2025): ISSUE JANUARY-JUNE 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v13i1.58358

Abstract

Plastic waste is an environmental issue, including in marine environments. One method of managing plastic waste is through biodegradation using bacteria. This study aims to isolate bacteria from plastic waste in Malalayang, and North Sulawesi waters, and test their potential to degrade plastic using laboratory experimental methods. Bacteria were isolated using a Nutrient Agar (NA) medium and then tested for their ability to degrade plastic using a Nutrient Broth (NB) medium containing plastic fragments. The NB medium with plastic fragments was incubated using an orbital shaker at room temperature with agitation at 130 rpm for 30 days. Five bacterial isolates were successfully obtained from plastic waste: isolates C1, C2, C3, C4, and C5. Four of the bacterial isolates were found to be capable of degrading plastic, as indicated by the reduction in the dry weight of the plastic after 30 days of incubation. The highest plastic weight reduction was shown in isolate C2, with a decrease of 2.95%, while the lowest reduction was observed in isolate C4, with a decrease of 2%. Keywords: Bacteria, Isolation, Biodegradation, Plastic. Abstrak Sampah plastik merupakan masalah lingkungan termasuk lingkungan laut. Salah satu pengelolaan sampah plastik adalah melalui biodegradasi menggunakan bakteri. Penelitian ini bertujuan mengisolasi bakteri dari sampah plastik Perairan Malalayang, Sulawesi Utara dan melakukan uji potensi bakteri tersebut dalam mendegradasi plastik menggunakan metode eksperimen laboratorium. Bakteri diisolasi menggunakan medium Nutrient Agar (NA), kemudian diuji kemampuan mendegradasi plastik dengan menggunakan medium Nutrient Broth (NB) yang berisi potongan plastik. Medium NB berisi potongan plastik diinkubasi menggunakan orbital shaker pada suhu ruang dengan agitasi 130 rpm selama 30 hari. Lima isolat bakteri berhasil diisolasi dari sampah plastik, yaitu isolat C1, C2, C3, C4 dan C5. Didapatkan empat isolat bakteri yang mampu mendegradasi plastik, yang ditunjukkan dengan pengurangan berat kering plastik setelah diinkubasi selama 30 hari. Pengurangan berat plastik tertinggi ditunjukkan oleh isolat C2 sebesar 2,95 %, sedangkan pengurangan berat terendah oleh isolat C4 sebesar 2 %. Kata kunci: Bakteri, Isolasi, Biodegradasi, Plastik.
Co-Authors , Sandra Tilaar Abrianto A. O. Rompis Agung B. Windarto Agustian Peloa, Agustian Angkouw, Esther Angmalisang, Ping Astony Annisaqois, Manikmayang Antonius P. Rumengan Asy'ari, Asy'ari Bella Wondal Billy Theodorus Wagey Calvyn F. A. Sondak, Calvyn F. A. Carolus Paulus Paruntu Chatrien Anita Sinjal, Chatrien Anita Cysca Lumenta Darus S. Paransa Darus Saadah J. Paransa Deiske A. Sumilat Deiske Adeliene Sumilat, Deiske Adeliene Edwin L.A. Ngangi Elvi L. Ginting Elvy Ginting Elvy L. Ginting Elvy L. Ginting Elvy L. Ginting, Elvy L. Elvy Like Ginting Elvy Like Ginting Erly Kaligis Erly Kaligis Erly Kaligis Fatti, Clara Ferdinand Frans Tilaar Fitje Losung Fitje Losung Fitje Losung Fitje Vera Losung Frista Tarigan Gian Losung Ginting, Elvy Ginting, Elvy Like Gledys Giacinta Poluan Grevo S Gerung Grevo S. Gerung Hengkengbala, Irvan R Henki Manoppo Henki Manoppo Henky Manoppo Henneke Pangkey Hens Onibala Herlina Pasaribu Herlina Pasaribu Hety B Lahope Indri Manembu Inneke F. M Rumengan Inneke Rumengan Inneke Rumengan James J.H. Paulus Jhon L. Tombokan Jhonly Solang Joice R.T.S.L Rimper Joice Rimper Joice Rimper Joshian N.W. Schaduw Julius Sampekalo Kurniati Kemer Laurentius T. X. Lalamentik Letha L. Wantania Liviani Rangian Mamuaja, Jane Marianne Mandagi, Stephanus Mangindaan, Remy Emile Petrus Mantiri, Desy M. H Medy Ompi Meiske Salaki Mikhael P. Pinontoan Mokoginta, Junio Marzuki Pratama Mokolensang, Jeffrie F. Mokosuli, Febrianty Dhea Mopay, Maratade N. Gustaf F. Mamangkey Natalie D Rumampuk Nia Nancy Kano Nickson J. Kawung Ode Mantra, Syahrun Palungan, Irpan Pankie Pangemanan Pankie Pangemanan Paulus, James Petrus P Letsoin Pipih Suptijah Purniasih, Ni Komang Pitri Reiny A Tumbol Reiny A. Tumbol Reiny Tumbol Reiny Tumbol Remy E. P Mangindaan Remy E.P. Mangindaan Remy E.P. Mangindaan Riorifki Kabense Rizald M. Rompas Rizald Max Rompas, Rizald Max Rizky I. Moroki Robert A. Bara Rondonuwu, Arie B. Rosita A.J. Lintang Rumampuk, Natalie Detty Rumampuk, Natalie Detty C. Sahari, Jefri Salaki, Meiske S. Sandra O. Tilaar Sandra Olivia Tilaar Sandra Tilaar Sandra Tilaar Sartje Lantu Sembiring, Sindiy Cloudya Silvester B Pratasik Singon, Cristio Smolak, Radoslav Sultan, Makkulau Suzanne L. Undap Talumepa, Anggun C. N. Tamara Angela Gabriela Siahaan Tindi, Monalisa Unstain N. W. J. Rembet, Unstain N. W. J. Veibe Warouw Veibe Warouw Veibe Warouw Veibe Warouw Wantania, Letha L. Wehantouw, Andre Wilmy E. Pelle