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All Journal The Indonesian Biomedical Journal Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) Proceedings of the International Conference on Nursing and Health Sciences Cermin Dunia Kedokteran Sehat Rakyat: Jurnal Kesehatan Masyarakat Smart Society Empowerment Journal Biocaster : Jurnal Kajian Biologi Promotor: Jurnal Mahasiswa Kesehatan Masyarakat Media Penelitian dan Pengembangan Kesehatan Indonesian Journal of Pharmacology and Therapy
Ratih Dewi Yudhani
Sebelas Maret University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Veterinary Other

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The Liver histopathology structure of Wistar rats on the acute toxicity test of Kapulaga Seeds extract (Amomum cardamomum) Ratih Dewi Yudhani; Rizka Hendriyani; Riza Novierta Pesik
Indonesian Journal of Pharmacology and Therapy Vol 1 No 2 (2020)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.717

Abstract

One-third of the Indonesian population uses herbs for alternative medicine empirically. One of them is Kapulaga (Amomum cardamomum) which known as a spice and traditionally used for the treatment of rheumatic, fever, cough, sore throat, and mouth odor. The clinical use of herbs must be supported by scientific evidenced-based and its safety profile. The risk of exposure to a substance in humans can be identified through the toxicity tests on animals. The liver histopathological structure is one of the parameters of the acute toxicity test. This study to determine the acute toxicity of Kapulaga seeds extract (Amomum cardamomum) based on the liver histopathological structure of Wistar rats. The sample selection based on inclusion and exclusion criteria in the OECD 420 guideline. The research consisted of a preliminary and the main test. One rat was used in the preliminary test with an initial dose of cardamom seed extract 300 mg/kg BW and followed by a maximum dose of 2000 mg/kg BW after 48 hours. The main test used 10 rats that were randomly divided into control and treatment groups. Based on the result of the preliminary test, the treatment group was given a single dose of cardamom seed extract 2000mg /kg BW, whilst the control group was given distilled water. Observations of any toxic signs or dead were conducted every 30 minutes in the first 4 hours and continued once per day until 14thday. Assessment of the degree of liver damage was done using the liver damaged scoring and compared using the Mann Whitney test (α = 0.05). The average liver damage score of control and treatment groups is 0.96 ± 0,856 and 0.96 ± 0,604, respectively, and did not show any statistically significant differences (p>0,05). Kapulagaseeds (Amomum cardamomum) had no acute toxic effect based on the liver histopathological structure of Wistar rats.
Systematic Review: Peran Sel, Jaringan, dan Kolagen dalam Penyembuhan Perforasi Membran Timpani Antonius Christanto; Risya Cilmiaty; Made Setiamika; Ratih Dewi Yudhani; Paramasari Dirgahayu
Sehat Rakyat: Jurnal Kesehatan Masyarakat Vol. 4 No. 4 (2025): November 2025
Publisher : Yayasan Pendidikan Penelitian Pengabdian Algero

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54259/sehatrakyat.v4i4.5771

Abstract

Tympanic membrane perforation is a significant clinical concern that can lead to hearing impairment and recurrent ear infections. This study aims to review the roles of Interleukin-1 beta (IL-1β), Fibroblast Growth Factor (FGF), fibroblasts, keratinocytes, granulation tissue, and collagen density during the wound-healing phases of the tympanic membrane.A Systematic Journal Review was conducted by analyzing studies retrieved from PubMed, Scopus, ScienceDirect, and Google Scholar databases published between 2020 and 2025. Of the 400 records screened,, 10 studies met the inclusion criteria. The findings revealed that IL-1β plays a key role in the inflammatory phase by modulating immune responses and recruiting healing cells. FGF stimulates fibroblast and keratinocyte proliferation, which are essential for extracellular matrix (ECM) remodeling and re-epithelialization. Moreover, granulation tissue formation and collagen deposition are critical determinants of structural integrity and healing quality. Overall, tympanic membrane repair requires synergistic interactions among molecular mediators, cellular components, and biomaterial support to optimize tissue regeneration. These findings provide a scientific basis for developing more effective regenerative and tissue-engineering strategies for middle ear therapy.
Curcumin compounds and total microorganisms in turmeric kombucha as a potential therapy in rats obesity model Nisa', Zulfia Rosyidatun; Jusuf, Sinu Andhi; Yudhani, Ratih Dewi
Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) VOLUME 13 ISSUE 2, 2025
Publisher : Alma Ata University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21927/ijnd.2025.13(2).114-124

Abstract

ABSTRAKLatar belakang: Prevalensi obesitas meningkat signifikan dalam satu dekade terakhir dan berisiko menimbulkan berbagai komplikasi seperti diabetes melitus tipe 2, kardiovaskular, bahkan kanker. Terapi farmakologi obesitas seperti liraglutide dan orlistat dapat memberikan efek negatif pada tubuh seperti gangguan saluran pencernaan. Hal ini mendorong pengembangan terapi berbahan alami untuk obesitas. Kombucha dan kunyit kuning secara tunggal dilaporkan bersifat antioksidan dan mampu memperbaiki obesitas. Kombinasi kombucha kunyit kuning berpotensi dikembangkan sebagai minuman probiotik untuk obesitas karena senyawa bioaktif yang dikandungnya. Namun, eksplorasi fitokimia dan mikroorganisme pada turmeric kombucha masih sangat terbatas dan penelitian kombinasi kombucha kunyit kuning untuk obesitas belum pernah dilakukan sebelumnya.Tujuan: Mengeksplorasi kandungan fitokimia dan total mikroorganisme kombucha kunyit kuning dan kemampuannya untuk memperbaiki obesitas. Metode: Eksperimental laboratorik dengan rancangan pre-post control group dan deskriptif observasional. Analisis kurkumin menggunakan KLT Densitometri. Mikroorganisme dianalisis dengan metode pour plate kemudian dihitung berdasarkan total plate count. Tikus model obesitas diberikan tiga variasi dosis kombucha kunyit kuning selama 28 hari dan dilakukan penilaian perubahan indeks Lee yang diuji statistik menggunakan Kruskal Wallis (p<0.05) dan Post hoc Dunn Test. Hasil: Hasil penelitian menunjukkan kandungan kurkumin pada kombucha kunyit kuning sebesar 0,73 mg/L dan total mikroorganisme sebesar 2,60 x 107 CFU/ml. Kombucha kunyit kuning memperbaiki indeks Lee tikus model obesitas dibanding kontrol negatif (p<0.05) dengan dosis paling efektif sebesar 4,4 ml/200 g/hari (dosis tertinggi di penelitian ini). Kesimpulan: Kombucha kunyit kuning memiliki kandungan kurkumin dan manfaat mikoorganisme yang dapat memperbaiki indeks Lee pada tikus model obesitas. Kata Kunci: kombucha kunyit kuning; kurkumin; mikroorganisme; obesitas   ABSTRACT Background: The prevalence of obesity has increased significantly in the last decade, raising the risk of complications such as type 2 diabetes, cardiovascular diseases, and even cancer. Pharmacological therapies for obesity, such as liraglutide and orlistat, can have negative effects on the body, including gastrointestinal disorders. This drives interest in the development of natural-based therapies for obesity. Kombucha and turmeric individually are known for their antioxidant properties and their ability to address obesity. The combination of turmeric kombucha has the potential to be developed as a probiotic drink for obesity due to the bioactive compounds it contains. However, research into the phytochemical profile and microorganisms in turmeric kombucha is still limited, and no studies have been conducted on the combination of turmeric kombucha for obesity treatment.Objectives: To explore the phytochemical content and total microorganisms in turmeric kombucha and to evaluate its potential in improving obesity treatment.Methods: Laboratory experiments were conducted with pre-post control group design and descriptive observational analysis. Curcumin levels were analysed using KLT Densitometry, while microorganisms were identified through the pour plate method and quantified via total plate count. Obese model rats received three different doses of turmeric kombucha over 28 days, and changes in their Lee index value were assessed and statistically analysed using Kruskal Wallis (p<0.05) and Post hoc Dunn Test. Results: The findings revealed that the curcumin concentration in turmeric kombucha was 0.73 mg/L and the total microorganisms were 2,60 x 107 CFU/ml. Kombucha yellow turmeric improved the Lee index of obese model rat compared to negative control (p<0.05) with the most effective dose being 4.4 ml/200 g/day (the highest dose in this study).Conclusions: Turmeric kombucha contains curcumin and beneficial microorganisms, which can improve the Lee index in obese model rats.Keywords: curcumin; obesity; microorganisms; turmeric kombucha Received: 17 Jan 2025; Revised: 20 Sep 2024; Accepted: 26 Jan 2025; Available online: 30 Mar 2025; Published: 30 Mar 2025
Red Rice Bran Ethanol Extract Reduces IL-1β as the Risk of Pancreas Fibrogenesis in Type 2 Diabetic Rat Model Brian Wasita; Ratih Dewi Yudhani; Dyonisa Nasirochmi Pakha; Diana Nurrohima; Muthmainah Muthmainah; Nanang Wiyono; Shariza Fakurazi
The Indonesian Biomedical Journal Vol 17, No 1 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i1.3410

Abstract

BACKGROUND: Oxidative stress and inflammation contribute to pancreatic cell dysfunction that promote insulin resistance in type 2 diabetes (T2D). Red rice bran contains bioactive substances with anti-inflammatory and antioxidant properties which improved insulin resistance in obese mice. However, no studies have explored the potential of ethanol extract of red rice bran (EERRB) to prevent T2D progression, particularly pancreatic fibrosis complications. This study was conducted to investigate the effect of EERRB on inflammation measured with interleukin (IL)-1β and fibrosis of pancreatic tissue in a rat model of T2D. METHODS: Rats were induced with streptozotocin and nicotinamide to induce diabetes, and then separated into five groups. One group received no treatment, while the other four received 9 mg/kg/day acarbose, 165, 330, or 660 mg/kg/day EERRB orally for 21 days. Immunohistochemistry was conducted on pancreas tissues to measure the expression of IL-1β, while pancreatic fibrosis was assessed with Masson’s Trichrome staining.RESULTS: EERRB reduced the expression of pro-inflammatory cytokine, IL-1β, in pancreas tissue in a dose dependent manner. Significantly lower IL-1β expression were found in group receiving 660 mg/kg/day EERRB (10%) compared to diabetic with no treatment group (50%) (p<0.0001). Additionally, the IL-1β expression in the highest dose of EERRB group was comparable to the group receiving acarbose (10%). CONCLUSION: This finding suggests the beneficial effect of EERRB in the hyperglycemic condition that causes oxidative stress through blocking the IL-1β expression, hence alleviating the inflammation in pancreas tissue, and have a tendency in preventing pancreatic fibrosis progression, a process implicated in T2D pathogenesis. KEYWORDS: diabetes, inflammation, pancreatic fibrosis, red rice bran
Systematic Journal Review: Mechanical Durability Of L-PRF Organic Material At Body Temperature During The Wound Healing Phase (14 Days) Of Tympanic Membrane Perforation And Myringoplasty Surgery Christanto, Antonius; Cilmiaty, Risya; Setiamika, Made; Yudhani, Ratih Dewi; Dirgahayu, Paramasari; Pamungkasari, Eti Poncorini; Nurwati, Ida
PROMOTOR Vol. 9 No. 1 (2026): FEBRUARI
Publisher : Universitas Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/pro.v9i1.1741

Abstract

The purpose of this study is to evaluate the viability and structural stability of L-PRF at 37°C over 14 days. It aims to determine its sustained growth factor release and potential effectiveness in regenerative clinical applications. This research employed a Systematic Journal Review (SJR) approach, surveying articles from databases such as PubMed, Scopus, and Google Scholar using specific keywords related to L-PRF. A total of 25 articles meeting the inclusion criteria were filtered for analysis. Furthermore, common experimental methods described in the literature include the preparation of L-PRF via blood centrifugation and viability testing over 14 days at 37°C, encompassing analysis of microscopic structure, mechanical properties, and biochemical and biological activities. Literature analysis indicates that L-PRF is an effective autologous biomaterial for regenerative applications, capable of continuously releasing growth factors such as PDGF, TGF-β, VEGF, and IGF for up to 14 days at body temperature. Cell viability within the fibrin matrix is maintained, positioning L-PRF as a promising active biological scaffold for accelerating tissue healing. Although challenges such as matrix degradation persist, modification strategies and optimized centrifugation protocols can enhance the effectiveness and durability of L-PRF in clinical applications.
Keunggulan L-PRF Dibandingkan P-PRF, PRFM, A-PRF, & I-PRF sebagai Cangkok pada Operasi Miringoplasti: Studi Penyembuhan Luka pada Perforasi Membran Timpani Antonius Christanto; Risya Cilmiaty; Made Setiamika; Ratih Dewi Yudhani; Paramasari Dirgahayu
Sehat Rakyat: Jurnal Kesehatan Masyarakat Vol. 5 No. 1 (2026): Februari 2026
Publisher : Yayasan Pendidikan Penelitian Pengabdian Algero

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54259/sehatrakyat.v5i1.6020

Abstract

This study aims to analyze the advantages of Leukocyte-Platelet Rich Fibrin (L-PRF) application in myringoplasty surgery compared to other platelet concentrates such as Platelet-Rich Fibrin Membrane (PFRM), Advanced Platelet-Rich Fibrin (A-PRF), and Injectable Platelet-Rich Fibrin (I-PRF). The research method used is a qualitative literature review, collecting and analyzing data from recent scientific journals and clinical studies related to the use of L-PRF in tympanic membrane repair. The study finds that L-PRF, with its dense fibrin network and high content of leukocytes and growth factors, enhances tissue regeneration, angiogenesis, and epithelialization, which are critical for successful graft integration and faster healing after myringoplasty. Compared to other platelet concentrates, L-PRF offers better mechanical strength and a more sustained release of growth factors. Additionally, L-PRF reduces postoperative complications and improves hearing outcomes. Being an autologous biomaterial, it also reduces the risk of immune rejection. The findings suggest that L-PRF is a promising graft material in otologic surgery, though further clinical trials are needed to optimize its application protocols.
Vitamin D and Magnesium Combination Lowers High-Sensitivity C-Reactive Protein, Interleukin-12, Renin, and Carotid Vessel in Diabetic Rats Rahayu, Rachmi Fauziah; Zahrah, Fathimah Afifah; Anugrah, Tessa Septian; Yudhani, Ratih Dewi; Yueniwati, Yuyun; Irawan, Amelia Tjandra; Kusumaningrum, Sulistyani; Fauzi, Adam; Wasita, Brian; Wiyono, Nanang; Muthaminah, Muthaminah; Sholikah, Tri Agusti; Wardhani, Lusi Oka; Suyatmi, Suyatmi; Yarso, Kristanto Yuli; Pesik, Riza Novierta; Siswanto, Febby Gunawan
The Indonesian Biomedical Journal Vol 18, No 2 (2026)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v18i2.4061

Abstract

BACKGROUND: Diabetes mellitus induces oxidative stress, inflammation, and endothelial dysfunction, accelerating atherosclerosis development marked by elevated high-sensitivity C-reactive protein (hs-CRP) and interleukin-12 (IL-12). Diabetes often causes significant vascular changes, which can be represented by carotid tunica intima thickening and elevated renin level. Metformin, which remains as the standard therapy, has limited vascular effects. Vitamin D and magnesium exhibit vasoprotective and anti-inflammatory properties; however, their combined effects on diabetic vascular injury remain unclear. This study evaluated the effects of vitamin D and magnesium combination on serum hs-CRP, IL-12, renin, and carotid tunica intima thickness in diabetic rats.METHODS: Wistar rats were diabetic-induced by 45 mg/kg streptozotocin and 110 mg/kg nicotinamide. Following the induction, the rats were subsequently assigned to receive no treatment, metformin, or a combination of vitamin D and magnesium. Healthy rats were also included as control. Blood sample was collected after 21 days of treatment. Serum hs-CRP, IL-12, and renin were measured using enzyme-linked immunosorbent assay (ELISA), while the carotid tunica intima thickness was assessed by Doppler ultrasound.RESULTS: Diabetic rats showed significant increases hs-CRP, IL-12, renin level, and intima thickness (p<0.001) compared to control. Both metformin and combination of Vitamin D and magnesium reduced these parameters, with combination of Vitamin D and magnesium showing the greatest improvement. Carotid intima thickness in the combination of Vitamin D and magnesium group was comparable to normal controls, indicating marked vascular protection.CONCLUSION: Vitamin D and magnesium combination provided superior glycemic, anti-inflammatory, and vascular protective effects compared to metformin alone. This adjunctive strategy might have potential in preventing vascular complications in diabetes.KEYWORDS: diabetes mellitus, oxidative stress, endothelial dysfunction, hs-CRP, IL-12, atherosclerosis
Pengetahuan, Sikap dan Perilaku Responden di Fakultas Kedokteran UNS Terhadap Penyakit Hepatitis Yudhani, Ratih Dewi; Sari, Yulia; Suryawati, Betty; Pakha, Dyonisa Nasirochmi
Smart Society Empowerment Journal Vol 5, No 2 (2025): Juli
Publisher : Faculty of Medicine, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/ssej.v5i2.103503

Abstract

Pendahuluan: Indonesia merupakan daerah endemis menengah hingga tinggi untuk hepatitis B, dimana  prevalensi mencapai 7,1%. Sementara prevalensi hepatitis C diperkirakan sekitar 1%, namun pada kelompok populasi berisiko seperti pengguna narkoba suntik, pasien hemodialisis, dan narapidana angkanya dapat lebih tinggi.Metode: Penelitian ini merupakan penelitian analitik deskriptif mendapatkan gambaran awal pengetahuan, sikap dan perilaku mahasiswa, pegawai administratif dan pegawai cleaning service di FK UNS terkait penyakit hepatitis. Desain penelitian ini adalah cross sectional dengan reponden 29 orang yang telah memenuhi keriteria inklusi, eksklusi dan telah menandatangani informed consent. Instrumen yang digunakan kuesioner pengetahuan, sikap dan perilaku terkait penyakit hepatitis.Hasil dan pembahasan: Hasil penelitian menunjukkan jumlah responden berjenis kelamin laki-laki sebesar 55,17% dengan tingkat pendidikan terakhir adalah SMA sebesar 68,97% dan sudah pernah mendapatkan informasi terkait penyakit hepatitis. Tingkat pengetahuan responden terhadap penyakit hepatitis sebesar 79,31% memiliki tingkat pengetahuan yang baik. Hasil penilaian sikap responden terhadap penyakit hepatitis menunjukkan 89,66% telah memiliki sikap yang baik. Sikap baik tersebut meliputi percaya bahwa hepatitis merupakan penyakit yang serius, merasa perlu mengetahui lebih banyak tentang hepatitis, kesediaan untuk vaksinasi hepatitis B jika tersedia, merasa penting untuk mendorong keluarganya melakukan pemeriksaan hepatitis, dan merasa khawatir jika teman atau keluarga menderita hepatitis. Hasil penelitian ini menunjukkan meskipun sebagian besar responden memiliki tingkat pengetahuan dan sikap yang baik, namun 72,41% responden berperilaku terhadap pencegahan penularan hepatitis masih tergolong kurang baik.Kesimpulan: Tingkat pengetahuan dan sikap yang baik terkait penyakit hepatitis, namun perilaku terhadap pencegahan penularan hepatitis masih tergolong kurang baik. Meskipun masih merupakan gambaran awal dengan responden terbatas, namun dapat mengindikasikan pentingnya intervensi multifaktorial dalam pengendalian hepatitis.
Co-Authors Abdurahim, Ghani Adam Fauzi Afiono Agung Prasetyo Antonius Christanto Antonius Christanto Anugrah, Tessa Septian Aurika, Denalia Brian Wasita Brian Wasita Brian Wasita Christanto, Antonius Denalia Aurika Diana Nurrohima Dyonisa Nasirochmi Pakha Eti Poncorini Pamungkasari Fauziah, Melinda Ghani Abdurahim Ida Nurwati Irawan, Amelia Tjandra Jusuf, Sinu Andhi Jusuf, Sinu Andhi Khalisah Atma Aulia Kristanto Yuli Yarso Kusumaningrum, Sulistyani KUSUMAWATI, RATNA Leli Saptawati LISANA SHIDQI Luthfiani, Sarah Made Setiamika Made Setiamika Muthaminah, Muthaminah Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah, Muthmainah Nanang Wiyono Nanang Wiyono Nanang Wiyono Natasha Ninda Pramalista Nisa', Zulfia Rosyidatun Nisa', Zulfia Rosyidatun Nisa, Zulfia Rosyidatun NOVAN ADI SETYAWAN Novan Adi Setyawan Paramasari Dirgahayu Paramasari Dirgahayu Pramalista, Natasha Ninda Prasetyo, Afiono Agung Rachmi Fauziah Rahayu Ratna Kusumawati RENDRA RISTIAN WIBOWO Reviono - Risya Cilmiaty Risya Cilmiaty Risya Cilmiaty, Risya Riza Novierta Pesik Riza Novierta Pesik Riza Novierta Pesik Rizka Hendriyani Rizka Hendriyani Saptawati, Leli Sarah Luthfiani Setiamika, Made Shariza Fakurazi SHIDQI, LISANA Sholikah, Tri Agusti Sinu Andhi Jusup, Sinu Andhi Siswanto, Febby Gunawan Siti Ma&#039;rufah SURYAWATI, BETTY Susanto, Agung Suyatmi Suyatmi Suyatmi Suyatmi Wahyu Tri Kawuri Wardhani, Lusi Oka Yarso, Kristanto Yuli Yulia Sari Yuyun Yueniwati Zahrah, Fathimah Afifah