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All Journal HAYATI Journal of Biosciences Majalah Obat Tradisional The Indonesian Biomedical Journal JOIV : International Journal on Informatics Visualization JSFK (Jurnal Sains Farmasi & Klinis) FITOFARMAKA: Jurnal Ilmiah Farmasi Majalah Patologi Indonesia Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Narra J Indonesian Journal of Cancer Jurnal Riset Rumpun Ilmu Kedokteran (JURRIKE) The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Indonesian Journal of Medical Chemistry and Bioinformatics Journal of Innovative and Creativity Jurnal Kefarmasian Indonesia
Kusmardi Kusmardi
Departemen Patologi Anatomik, Fakultas Kedokteran, Universitas Indonesia Jakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : Narra J

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Beetroot (Beta vulgaris) potential in preventing colorectal cancer using in-silico analysis Dwijayanti, Adisti; Azizah, Norma N.; Erlina, Linda; Kusmardi, Kusmardi; Ningsih, Sri S.; Fadilah, Fadilah; Hashim, Najihah M.
Narra J Vol. 5 No. 2 (2025): August 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i2.1578

Abstract

Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality worldwide, necessitating the need for an effective therapeutic strategy. Beta vulgaris (beetroot) possesses active compounds that exert anti-cancer properties. The aim of this study was to evaluate the potential of beetroot as a preventative agent against the progression of CRC using differentially expressed gene (DEG) analysis and network pharmacology approaches. The protein-protein interaction network and molecular docking analyses were employed to assess the key interactions of beetroot active compounds with CRC-related target protein. Cytotoxicity of beetroot extract was experimentally evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay on the HT29 cell line. The result of this study showed that protein in the cell cycle was significantly enriched in CRC, with cyclin-dependent kinase 4 (CDK4) gene as one of the specific genes. Quercetin, galangin, hesperidin, farrerol, and betanin were the most typical compounds of beetroot based on the Comparative Toxicogenomics Database (CTD). Molecular docking studies revealed the strong binding affinity between quercetin (-7.04 kcal/mol) and bentanin (-8.11 kcal/mol) with CDK4. Beetroot demonstrated anticancer properties against the HT29 cell line with IC50 value of 39.03±1.4 µg/mL. In conclusion, the beetroot extract has inhibitory activity against HT29 cell line proliferation, highlighting its potential in preventing the development of CRC through the substantial suppression of gene expression within the cell cycle pathway.
Co-Authors . Syamsudin Adisti Dwijayanti Alfred Pakpahan, Alfred Aniati Murni Anwar S. Ibrahim Anwar S. Ibrahim Aprilliyani, Ifana Ariadi Humardhani Ariffandi, Bagas Arleni Bustami Aryo Tedjo Athifa, Khasna Fayza Atmaja, Fredy Wirya Azizah, Norma N. Berna Elya Budiana Tanurahardja Dewi Sukmawati Diah Rini Handjari Dian Ratih Laksmitawati Dimas Ramadhian N Ditha Diana Ditha Diana Dwira, Surya Dwitia Wulandari Endang SR. Hardjolukito Endang Sri Roostini Hardjolukito Enif Esti Triana Ening Krisnuhoni Erlina, Linda Erwina Muhadi Fadhillah, Muhamad Rizqy Fadilah Fadilah, Fadilah Fajriati, Annisa Hamdani Zain Hanum, Sitti Fatimah Hashim, Najihah M. Hayun Hayun Hellyanti, Tantri Hendig Winarno Heri Wibowo Heru Suhartanto Hikariastri, Pangartika Imelda Setiana Silitonga Irandi Putra Pratomo Ismalasari, Wahyu Jasni Mohammad Zain Khatimah, Nurul Gusti Kristanty, Diyah Ladeska, Vera Landyyun Rahmawan Sjahid Landyyun Rahmawan Sjahid Lestari, Tri Wahyuni Lili Indrawati Linggodigdo, Michelle Lubis, Nadar Sukri Megawati, Ajeng Muhammad Hanafi Murtiani, Farida Nina Yolanda Putri Noor, Dimas Ramadhian Novi Fajar Utami Nurdin, Wildawati Peni Kistijani Samsuria Putri Reno Intan Putri, Rizky Clarinta Rani Wardani Hakim Rino Patiatta Rohmat, Siti Saudah Shirly Kumala Sibli, Sibli Sri S. Ningsih, Sri S. Sri Suryo Adiyanti, Sri Suryo Surya Dwira Syamsu Nur, Syamsu Toto Haryanto Triana, Novi Virhan Novianry Vivi Setiawaty Wahyu Hidayati Wahyu Hidayati Wahyu Ismalasari Wawaimuli Arozal Widiantari, Aninda Dinar Wiwiek Ernajanti Wiyanto, Marwito Yulhasri Yulhasri Yusoff, Marina Yusra Yusra