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All Journal Indonesia Medicus Veterinus Biology, Medicine, & Natural Product Chemistry Aceh Journal of Animal Science Medical Laboratory Technology Journal JMM (Jurnal Masyarakat Mandiri) Global Health Management Journal Oceana Biomedicina Journal Jurnal Sains dan Teknologi Peternakan Community Empowerment OISAA Journal of Indonesia Emas Journal of Experimental and Clinical Pharmacy (JECP) Pengabdian Kampus: Jurnal Informasi Kegiatan Pengabdian Pada Masyarakat Kontribusi: Jurnal Penelitian dan Pengabdian Kepada Masyarakat Oceana Biomedicina Journal International Journal of Sustainable Applied Sciences (IJSAS) Media Kesehatan Politeknik Kesehatan Makassar Barigas: Jurnal Riset Mahasiswa Tropis: Jurnal Riset Teknologi Laboratorium Medis Mosaic: Jurnal Pengabdian Kepada Masyarakat Al-Musthalah: Jurnal Riset dan Penelitian Multidisiplin
Rian Ka Praja
Program Studi Kedokteran, Fakultas Kedokteran Universitas Palangka Raya
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : Medical Laboratory Technology Journal

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The Effect of Methanol Fractıonated of Luvunga sarmentosa on In Vıtro Sperm Membrane Integrıty Permatasari, Silvani; Nuha, Alya Firyal; Ratnasari, Arini; Widayati, Ratna; Ka Praja, Rian
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.571

Abstract

Infertility cases in the world are increasing in 2020, with 186 million individuals affected worldwide. The most common cause of male infertility is due to decreased sperm quality. Sperm membrane integrity is known to play an essential role in the fertilization ability of spermatozoa. The methanol fraction of Luvunga sarmentosa root extract has the highest antioxidant activity compared to other fractions and can improve sperm motility in vitro. This study aims to determine the effect of the methanol fraction of Luvunga sarmentosa root extract on human sperm membrane integrity and the effective concentration of Luvunga sarmentosa methanol extract fraction to improve sperm membrane integrity in vitro. The samples used were washed human spermatozoa in vitro, then added BWW medium, and incubated at 37℃ for 1 hour with methanol fraction of Luvunga sarmentosa root extract at concentrations of 100, 500, 1000, and 5000ng/ml as well as the control group (BWW only). Furthermore, 100uL of each sample was added to 1mL of Hypo-osmotic swelling (HOS) solution, then incubated at 37oC for 30 minutes to see the integrity of the spermatozoa membrane from the tail swelling. The results showed that sperm membrane integrity significantly increased at 100, 500, 1000, and 5000ng/mL concentrations compared to the control group. The methanol fraction of Luvunga sarmentosa root extract was most effective at a concentration of 100ng/mL. Methanol fraction of Luvunga sarmentosa root extract can improve human sperm membrane integrity.
Co-Authors Akmal, Rizqy Fajar Syaikhul Amanda, Nadia Keisha Andra Ari P., Eko Marwanto Heru Mulyantoro Dwi Hermayantiningsih Angeline Novia Toemon, Angeline Novia Anggita Ratri Pusporini Anwar, Muhammad Naufa Arif Rahman Jabal Arini Ratnasari Astrid Ekkelsia Saputri Astrid Ekklesia Saputri Auliazar, Tarra Austin Bertilova Carmelita, Austin Bertilova Balyas, Abi Bakring Bustomi, Qonita Sari Cereymo Ewaldo Cici Meriliyani Cici Meyriliani Sensia Cristin, Mery Enjelica Decenly, Decenly Dinda Ananda Sulistina Dwi Putri Safnurbaiti Dwijayanti, Rts. Sherly Elsa Trinovita Estilia Ledyani Ewaldo, Cereymo Frethernety, Agnes Furtuna, Dewi Klarita Habib, Yusril Hanasia Hanasia Hanasia Hanasia, Hanasia Harianja, Henrigen Hartoyo, Riccardo Hasan, Muhamad Umar Muhtar Herlina Eka Shinta I Dewa Gede Sandhikarana I Dewa Made Sukrama I Wayan Muda Suta Arta I Wayan Suardana Jessica Elisabeth Suharis Kahanjak, Donna Novina Karamo, Beatrice Benita Komang Januartha Putra Pinatih Kurniawan, Mutiara Lauren Maretha Lavefolia, Raissa Amanda Ledyani, Estilia Lusi Ulina Simanungkalit Maretha, Lauren Margaretha Yayu Indah Anugerahny Marta Monika Simamora Mery Enjelica Cristin Muhammad Naufa Anwar Muhammad Naufa Anwar Muhammad Naufal Al-Firas Muhammad Ricko Fahrizal Nabilatul Zhofiroh Nadia Keisha Amanda Nadia Kesiha Amanda Br Ginting Nadiah Romadhona Putri Nasywa Putri Rahmadina Natalia Sri Martani Nawan Nawan, Nawan Nazhifah, Annida Kholifatun Ni Nengah Dwi Fatmawati Ni Nengah Dwi Fatmawati Ni Nyoman Sri Yuliani Nuha, Alya Firyal Nur Khalifah Putri Utami Nurul Ilma Haryono Permatasari, Silvani Purba, Yohana Catherine Debyola Putri, Nadiah Romadhona Qonita Sari Bustomi Rahmadina, Nasywa Putri Raissa Amanda Lavefolia Ranintha BR Surbakti Ranti, Dalia Reny Rosalina Reny Rosalina Reny Rosalina, Reny Riccardo Hartoyo Rizki Rachmad Saputra Rizqy Fajar Syaikhul Akmal Salma Fathinah Salsabila, Ivah Sandhikarana, I Dewa Gede Saputri, Astrid Ekklesia Sensia, Cici Meyriliani Simamora, Marta Monika Simanungkalit, Lusi Ulina Sintice Dhea Valerie Suharis, Jessica Elisabeth Suma, Joy Angel Aria Tarra Auliazar Teresa, Astrid Trisia, Adelgrit Turnip, Oktaviani Naulita Utami, Nur Khalifah Putri Valerie, Sintice Dhea Wahyu Hidayati Widayati, Ratna Widhiyanto, Hendrykus Theo Damar Widiarti, Astri Yan Antonio Yohana Catherine Debyola Purba Ysrafil, Ysrafil Yusril Habib