Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Medical Laboratory Technology Journal

The Effect of Methanol Fractıonated of Luvunga sarmentosa on In Vıtro Sperm Membrane Integrıty Permatasari, Silvani; Nuha, Alya Firyal; Ratnasari, Arini; Widayati, Ratna; Ka Praja, Rian
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.571

Abstract

Infertility cases in the world are increasing in 2020, with 186 million individuals affected worldwide. The most common cause of male infertility is due to decreased sperm quality. Sperm membrane integrity is known to play an essential role in the fertilization ability of spermatozoa. The methanol fraction of Luvunga sarmentosa root extract has the highest antioxidant activity compared to other fractions and can improve sperm motility in vitro. This study aims to determine the effect of the methanol fraction of Luvunga sarmentosa root extract on human sperm membrane integrity and the effective concentration of Luvunga sarmentosa methanol extract fraction to improve sperm membrane integrity in vitro. The samples used were washed human spermatozoa in vitro, then added BWW medium, and incubated at 37℃ for 1 hour with methanol fraction of Luvunga sarmentosa root extract at concentrations of 100, 500, 1000, and 5000ng/ml as well as the control group (BWW only). Furthermore, 100uL of each sample was added to 1mL of Hypo-osmotic swelling (HOS) solution, then incubated at 37oC for 30 minutes to see the integrity of the spermatozoa membrane from the tail swelling. The results showed that sperm membrane integrity significantly increased at 100, 500, 1000, and 5000ng/mL concentrations compared to the control group. The methanol fraction of Luvunga sarmentosa root extract was most effective at a concentration of 100ng/mL. Methanol fraction of Luvunga sarmentosa root extract can improve human sperm membrane integrity.
Co-Authors Akmal, Rizqy Fajar Syaikhul Amanda, Nadia Keisha Angeline Novia Toemon, Angeline Novia Anggita Ratri Pusporini Anugerahny, Margaretha Yayu Indah Anwar, Muhammad Naufa Arif Rahman Jabal Arini Ratnasari Auliazar, Tarra Austin Bertilova Carmelita, Austin Bertilova Balyas, Abi Bakring Bustomi, Qonita Sari Cristin, Mery Enjelica Decenly, Decenly Dwi Putri Safnurbaiti Dwijayanti, Rts. Sherly Elsa Trinovita Ewaldo, Cereymo Frethernety, Agnes Furtuna, Dewi Klarita Habib, Yusril Hanasia Hanasia, Hanasia Harianja, Henrigen Hartoyo, Riccardo Hasan, Muhamad Umar Muhtar I Dewa Gede Sandhikarana I Dewa Made Sukrama I Wayan Muda Suta Arta I Wayan Suardana Kahanjak, Donna Novina Karamo, Beatrice Benita Komang Januartha Putra Pinatih Kurniawan, Mutiara Lavefolia, Raissa Amanda Ledyani, Estilia Maretha, Lauren Muhammad Naufa Anwar Muhammad Ricko Fahrizal Nadia Kesiha Amanda Br Ginting Natalia Sri Martani Nawan Nawan, Nawan Nazhifah, Annida Kholifatun Ni Nengah Dwi Fatmawati Ni Nengah Dwi Fatmawati Ni Nyoman Sri Yuliani Nuha, Alya Firyal Permatasari, Silvani Purba, Yohana Catherine Debyola Putri, Nadiah Romadhona Rahmadina, Nasywa Putri Ranintha br Surbakti Ranti, Dalia Reny Rosalina Reny Rosalina Riccardo Hartoyo Rizki Rachmad Saputra Salsabila, Ivah Sandhikarana, I Dewa Gede Saputri, Astrid Ekklesia Sensia, Cici Meyriliani Simamora, Marta Monika Simanungkalit, Lusi Ulina Suharis, Jessica Elisabeth Sulistina, Dinda Ananda Suma, Joy Angel Aria Teresa, Astrid Trisia, Adelgrit Turnip, Oktaviani Naulita Utami, Nur Khalifah Putri Valerie, Sintice Dhea Wahyu Hidayati Widayati, Ratna Widhiyanto, Hendrykus Theo Damar Widiarti, Astri Yan Antonio Ysrafil, Ysrafil Zhofiroh, Nabilatul