Article Per Year (5 Year)
p-Index From 2021 - 2026
3.78
P-Index
This Author published in this journals
All Journal Majalah Kedokteran Bandung Indonesian Journal of Urology Berkala Ilmu Kesehatan Kulit dan Kelamin Majalah Obstetri dan Ginekologi Majalah Patologi Indonesia Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Layanan Masyarakat (Journal of Public Service) Jurnal Pengabdian Masyarakat dalam Kesehatan Indian Journal of Forensic Medicine & Toxicology Narra J Indonesian Journal of Cancer Jurnal Pengabdian Masyarakat Bhinneka Jurnal Pengabdian Masyarakat Bangsa Folia Medica Indonesiana
Gondo Mastutik
Departemen Patologi Anatomi, Fakultas Kedokteran, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

Published : 43 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 43 Documents
Search

 1   2   3   4   5 
The elevation of osteoblast activity in rat bone marrow mesenchymal stem cells in osteogenic medium exposed with melatonin in physiological doses Yuliyanasari, Nurma; Mastutik, Gondo; Putra, Suhartono Taat
Folia Medica Indonesiana Vol. 53, No. 1
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The objective of this study was to analyze the elevation of osteoblast activity in bone marrow mesenchymal stem cells (BM-MSCs) in osteogenic medium by physiological doses of melatonin administration by measuring alkaline phosphatase (ALP) and osteocalcin level.This studyused BM-MSCs from Rattusnorvegiccus femur bone. Rat BM-MSCs were cultured in a-Mem medium, differentiated in osteogenic medium, and administrated melatonin up to 21 days. This study was divided into 4 groups, K0 (control group), K1 (administrated of 25 nM melatonin), K2 (administrated of 50 nM melatonin), and K3 (administrated of 100 nM melatonin). Rat BM-MSCs were characterized CD 45- and CD 105+ marker using imunocytochemistry analysis and stained with Alizarin red after 15 days treatment. ALP and osteocalcin level were measured using ELISA Kit in days 21st.There weren't differences ofALP level beetwen groups and there are differences ofosteocalcin level between control groups (K0) withK1, K2, dan K3, and beetwen K1 and K2. The conclusion of this study was that there were an elevation of osteoblast activity in rat BM-MSCs in osteogenic medium by physiological doses of melatonin administration characterized by the elevation of osteocalcin level.
The Expression of E6 HPV, P53 and P16ink4a at Well, Moderately, and Poorly Differentiated Cervical Adenocarcinoma Mastutik, Gondo; Rahniayu, Alphania; Kurniasari, Nila; Rahaju, Anny Setijo; Alia, Rahmi; Mustokoweni, Sjahjenny
Folia Medica Indonesiana Vol. 55, No. 4
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Ethanol extract of leaves and petiole of pegagan (Centella asiatica) is potential as antimicrobial and antifertility. This study was to investigate whether ethanol extracts of leaf and petiole of Centella asiatica has potential as an antimicrobial and can decrease sperm quality and spermatogenic cells of mice. This was a randomized controlled study using Centella asiatica plant from which we made ethanol extract. The extract of the leaves and petiole of Centella asiatica was subjected to antimicrobial test using the bacteria S. aureus, S. epidermidis, and A. aerogenes. Then the observation was done after a 24-hour-old bacterial cultures by measuring the diameter of the clear zone in mm. Thereafter, the extract was given to randomly grouped mice that were divided into two groups receiving the leaf extract in one group and the petiole exract in another for 35 days. On day 36 the mice were sacrificed for spermatozoa quality and testicular histology examination. Statistical analyses used in this study were ANOVA and LSD tests. There was inhibition zone difference between leaf and petiole (p=0.000). Inhibition zone between bacteria S. epidermidis, S. aureus and A. aerogenes showed no difference (p=0.198). Inter-dose inhibition zone showed difference (p=0.000). Centella asiatica leaf extract showed inhibition zone better than that of Centella asiatica petiole extract. In spermatozoa quality test, there was effect of Centella asiatica leaf extract on sperm motility (p<0.05), viability (p<0.05), morphology (p<0.05) and concentration (p<0.05). No effect of pegagan petiole extract was found on sperm motility (p<0.05), viability (p<0.05), abnormal morphology (p<0.05) and concentration (p<0.05). Highest degradation of spermatozoa quality was obtained from Centella asiatica petiole extract at a concentration of 150 mg/kg bw. Spermatogenic cell test results showed no effect of Centella asiatica leaf extract in decreasing spermatogonia (p=0.000), spermatocytes (p=0.000), and spermatids (p=0.000) counts. No effect of pegagan petiole extract in decreasing the number of spermatogonia, spermatocytes and spermatids. The highest reduction spermatogenic cells of seminiferous tubules was obtained by Centella asiatica petiole extract in a concentration of 150 mg/kg bw. In conclusion: Centella asiatica leaf and petiole extract has antimicrobial potency as contra-ceptives
Expression of Melanoma Antigen Genes A11 and A12 in Non-Small Cell Lung Cancer Mastutik, Gondo; Rahniayu, Alphania; , Isnin Anang Marhana; Amin, Mochamad; Trianto, Heru Fajar; I'tishom, Reny
Folia Medica Indonesiana Vol. 59, No. 4
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Highlights: 1. In this study, new primers designed using the semi-nested polymerase chain reaction (PCR) method were utilized to identify MAGE-A11 and MAGE-A12 expressions in specimens collected from core biopsy, forcep biopsy, and bronchoalveolar lavage. 2. The histopathological analysis revealed positive expressions of MAGE-A11 and MAGE-A12 in specimens diagnosed with non-small cell lung cancer (NSCLC) as well as in specimens with no malignant cells. 3. This study provides evidence indicating that the detection of messenger ribonucleic acid (mRNA) of MAGE-A11 and MAGE-A12 by nested reverse transcription PCR can improve the accuracy of lung cancer diagnosis. Abstract The melanoma antigen gene (MAGE) belongs to the group of cancer-testis antigens that are exclusively expressed in germ cells but may be re-expressed in cancer cells. The highly expressed MAGE-A subfamily in lung cancer may potentially be a diagnostic and prognostic biomarker. This study aimed to identify MAGE-A11 and MAGE-A12 expressions in lung tumors obtained from core biopsy, forceps biopsy, and bronchoalveolar lavage specimens. A cross-sectional observational study was conducted on 90 patients clinically diagnosed with lung tumors. These patients received core biopsy, forceps biopsy, and bronchoalveolar lavage interventions after ethical approval was obtained. The complementary deoxyribonucleic acid (cDNA) quality was assessed by the polymerase chain reaction (PCR) of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The assessment was performed to ascertain if all specimens exhibited positive PCR amplification of the GAPDH gene. MAGE-A11 and MAGE-A12 were identified through a semi-nested reverse transcription PCR. The positive results were detected by measuring the PCR products, with MAGE-A11 and MAGE-A12 at base pairs (bp) of 858 and 496 in the first and second rounds, respectively. The expressions of MAGE-A11 and MAGE-A12 were observed in 3 (3.33%) and 40 (44.44%) out of 90 specimens, respectively. The prevalence rate of non-small cell lung cancer (NSCLC) was 31.11% (28/90). Among these cases, 3.57% (1/28) showed the expression of MAGE-A11, while 32.14% (9/28) exhibited the expression of MAGE-A12. Sixty-two (68.89%) out of 90 patients were diagnosed with no tumor cell malignancy. Out of 62 cases, 2 (3.23%) exhibited the expression of MAGE-A11, while 31 (50%) demonstrated the expression of MAGE-A12. MAGE-A11 and MAGE-A12 were detected in NSCLC and certain specimens with a pathological diagnosis that indicated the absence of malignant cells. In conclusion, MAGE A11 and MAGE A12 have potential markers to improve the pathological diagnosis of lung cancer. Further investigation is necessary to explore the expression of MAGE-A in correlation with lung cancer progression.
Co-Authors , Isnin Anang Marhana Afria Arista Afria Arista Afria Arista, Afria Agung Dwi Suprayitno Agus Santosa Agus Santosa Ali Rohman Alphania Rahniayu Aniek Meidi, Aniek Anny Setijo Rahaju, Anny Setijo Ariani, Grace Arifa Mustika Aryati , Aryati Aryati Aryati Aryati Aryati Bagus Setyoboedi Benediktus Yohan Bilqis Inayatillah Budi Harjanto Budi Harjanto Cempaka Harsa Sekarputri Dewi Sartika A. W. Dini Indah Berlianti Dwi Murtiastutik DYAH FAUZIAH, DYAH Erni J. Nelwan, Erni J. Erni Juwita Nelwan, Erni Juwita Faisal Amir Faisal Amir Faroek Hoesin Fira Soraya Heriyawati, Heriyawati Heru Fajar Trianto I'tishom, Reny Ilmiah, Khafidhotul Imam Susilo Indiastuti, Danti Nur Iqhrammullah, Muhammad Isnin Anang Marhana Jennifer Susanto Juniastuti Juniastuti Khafidhotul Ilmiah Khafidhotul Ilmiah Kuntaman Kuntaman Kusumastuti , Etty Hary Kusumastuti, Etty Hari KUSUMASTUTI, ETTY HARY Lasmauli Situmorang Leonita Agustin Hambalie M Hasinuddin Markus Kore Masyeni, Sri Mochamad Amin MOCHAMAD AMIN Mochamad Amin Muchlis AU Sofro Nabiha Missaoui Nila Kurniasari Nila Kurniasari Nurma Yuliyanasari, Nurma Poernomo Boedi Setiawan Prasetyo, Rizki Eko Purnama, Yugi Hari Chandra Purnomo, Windu Qonitatillah, Ana R. Tedjo Sasmono Rahmi Alia Rahmi Alia Rahmi Alia Rahmi Alia, Rahmi Renny I’tishom Ridholia Ridholia, Ridholia Rizki Eko Prasetyo S.Pd. M Kes I Ketut Sudiana . Sari, Aditya Sita Sasmono, R. Tedjo Shaohong Lai Sjahjenny Mustokoweni, Sjahjenny Sjamsul Arief Sofro, Muchlis AU Sofro, Muchlis AU. Sri Masyeni, Dewa Ayu Putri Suhartono Taat Putra Sunaryo Hardjowijoto Sutrisno Sutrisno Tamam Jauhar Trianto, Heru Fajar Trisniartami Setyaningrum Usman Hadi Willy Sandhika Windu Purnomo Wira Santoso Ongko Wiratama, Priangga Adi Yohan, Benediktus Yugi Hari Chandra Purnama