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Journal : UNEJ e-Proceeding

The Potency of Protein Extracts from Candida albicansas Bioreceptor on Immunosensor for Diagnosis of Candidiasis ., Masfufatun; Kumala, Noer; Baktir, Afaf
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Currently diagnosis of candidiasis still usingthe traditional standard blood culture method. The traditional method were less sensitive andtime consuming. The purpose of this research were to develope the more sensitive immunosensor based method, and to examine the potency of C. albicans protein extract as bioreceptor to  detect  C. albicans and its biofilm in the blood of candidiasis patients.The research methods include: (1) preparation of  digestive gland liquid of snail (Achatina fulica); (2) extraction of protein from C. albicans  through  enzimatis and mechanic methodsand (3) analyzing the protein extract as bioreseptor through immunodot assay.The research results showed that the snail enzymes has protein content 1.35 mg/ml and  specific activity 1.96 unit/mg. The snail enzyme hydrolyzed  the cell wall of C. albicans  with and without sonication, producedplanktonic extracell protein extract (PEP) and biofilmextracell proteinextract (PEB), planktonic intracell protein extract (PIP), and biofilmintracell protein extract (PIB), withprotein content 1.44; 1.29; 1.29 and 1.21 mg/ml respectively. Thebiofilm intracell protein (BIP) showed antigenic property towardantibody anti-Candida (positive control),giving red spot on imunodot assay. Immunodot assay can distinguishnegative control serum (health man) and positive Candidiasis control by using antigen 1 mg/ml and50ml serum. Keywords: C. albicans, candidiasis, biofilm, immunodot assay
The Potency of Protein Extracts from Candida albicansas Bioreceptor on Immunosensor for Diagnosis of Candidiasis Masfufatun .; Noer Kumala; Afaf Baktir
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Currently diagnosis of candidiasis still usingthe traditional standard blood culture method. The traditional method were less sensitive andtime consuming. The purpose of this research were to develope the more sensitive immunosensor based method, and to examine the potency of C. albicans protein extract as bioreceptor to  detect  C. albicans and its biofilm in the blood of candidiasis patients.The research methods include: (1) preparation of  digestive gland liquid of snail (Achatina fulica); (2) extraction of protein from C. albicans  through  enzimatis and mechanic methodsand (3) analyzing the protein extract as bioreseptor through immunodot assay.The research results showed that the snail enzymes has protein content 1.35 mg/ml and  specific activity 1.96 unit/mg. The snail enzyme hydrolyzed  the cell wall of C. albicans  with and without sonication, producedplanktonic extracell protein extract (PEP) and biofilmextracell proteinextract (PEB), planktonic intracell protein extract (PIP), and biofilmintracell protein extract (PIB), withprotein content 1.44; 1.29; 1.29 and 1.21 mg/ml respectively. Thebiofilm intracell protein (BIP) showed antigenic property towardantibody anti-Candida (positive control),giving red spot on imunodot assay. Immunodot assay can distinguishnegative control serum (health man) and positive Candidiasis control by using antigen 1 mg/ml and50ml serum. Keywords: C. albicans, candidiasis, biofilm, immunodot assay
Co-Authors Adi Candra, Adi AFAF BAKTIR akhmad sudibya, akhmad ALMAIDAH SAFITRI Amalia Nadiasari Amrulloh, Azmi Khalid Ananta Sandi Putra Ananta, Zahrah Aswa Andini Risma Ayunda Dewi Andriani, Chenny Aritania, Putu Oky Atik Sri Wulandari Ayly Soekanto Ayu Cahyani Noviana Budhi Setiawan Budhi Setiawan Budhi Setyawan Chenny A Wiyono Chenny Andriani Wiyono Dadik Raharjo Emilia Devi D.R, Emilia Devi Fardiansyah, Al Farizah El Husna Fehima Syachsiyat Alya Firdausi Nuzula Habibi, Miftahul Handy Arief HARMAN AGUSAPUTRA Harsono Harsono Haryanto, Loo Herliani, Olivia I Kadek Wawan Agus Wijaya I MADE DWIKY KARINA JAYA Inawati Inawati Inawati Inawati Indahasari, Noer Kumala Islam, Afif Fajrul Isnaeni, Mutia Jalal, Ismul JAMES HADIPUTRA SUNARPO Joeniarti, Elika Jonathan Kartika Ishartadiati Kuntaman Kuntaman Laili Shafarina Fauzan Laily, Aulia Nur Listyawati, Agusniar Furkani Loo Hariyanto Raharjo, Loo Hariyanto Lusiani Tjandra Meilya Putri Syahida Mentari, Andi Oktaviana Mohammad Ali Izzudin Muhammad Rozzan Prananda Narotama, Harya Ni Luh Narita Vijayanti Ni Made Putri Pradnyandari NI PUTU MANIK SURYANINGSIH NOER KUMALA Noer Kumala Noer Kumala Noer Kumala Indahsari, Noer Kumala NUGROHO EKO WIRAWAN BUDIANTO Nur Kumala Indahsari, Nur Kumala Nur Kumala, Nur Nurma Fitri Parawidnyaningsih, Putu Ayu Aryanda Pramita Laksitarahmi Isrianto Pratika Yuhyi Hernanda Putu Oky Ari Tania Qurraty’ain, Seryna Hasna Rachmawati Dwi Apriliyani Ratnasari, Diana Tri Rini Purbowati Rini Purbowati Riska Ammalia Putri Risnanda, Kadek Rio Setijowati, Eva Diah Soebandhi, Santirianingrum Sri Lestari Utami Sugiharto Suhartati Suhartati Suhartati Syahrefi Syahrefi TEOFILUS DANI PRASETYOADI Tikapa Muwawiyai Pigome Tri Juliati Tri Rahayuningsih Tri Ratnasari, Diana Utami, Sri Lestari Vini Anggraini Wahyuni Dyah Parmasari, Wahyuni Dyah Wibisono, Freegied Satriya Wibisono, Freshinta Jellia Yoga Yuniadi