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All Journal Jurnal Kedokteran Brawijaya Althea Medical Journal AGRIVITA, Journal of Agricultural Science Biology, Medicine, & Natural Product Chemistry PROSIDING SEMINAR KIMIA Jurnal Terapan Abdimas UNEJ e-Proceeding Berkala Kedokteran Jurnal Kimia Riset BIOMA Padjadjaran Journal of Dentistry Jurnal Ilmiah Kedokteran Wijaya Kusuma Jurnal Kedokteran dan Kesehatan Jurnal Biologi Tropis The Indonesian Journal of Infectious Diseases Interdental Jurnal Kedokteran Gigi (IJKG) Medika Kartika : Jurnal Kedokteran dan Kesehatan Abdimas UNWAHAS : Jurnal Pengabdian Masyarakat UNWAHAS Jurnal Abdidas Jurnal Pengabdian Masyarakat: Humanity and Medicine Journal of Multidisciplinary Applied Natural Science Berkala Penelitian Hayati Hang Tuah Medical Journal JAPI: Jurnal Akses Pengabdian Indonesia Prosiding Seminar Nasional Kusuma Prosiding Seminar Nasional COSMIC Kedokteran Public Health Journal JI-KES (Jurnal Ilmu Kesehatan)
Masfufatun Masfufatun
Departemen Biokimia, Fakultas Kedokteran, Universitas Wijaya Kusuma Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Journal : unej e-proceeding

 1 
The Potency of Protein Extracts from Candida albicansas Bioreceptor on Immunosensor for Diagnosis of Candidiasis ., Masfufatun; Kumala, Noer; Baktir, Afaf
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Currently diagnosis of candidiasis still usingthe traditional standard blood culture method. The traditional method were less sensitive andtime consuming. The purpose of this research were to develope the more sensitive immunosensor based method, and to examine the potency of C. albicans protein extract as bioreceptor to  detect  C. albicans and its biofilm in the blood of candidiasis patients.The research methods include: (1) preparation of  digestive gland liquid of snail (Achatina fulica); (2) extraction of protein from C. albicans  through  enzimatis and mechanic methodsand (3) analyzing the protein extract as bioreseptor through immunodot assay.The research results showed that the snail enzymes has protein content 1.35 mg/ml and  specific activity 1.96 unit/mg. The snail enzyme hydrolyzed  the cell wall of C. albicans  with and without sonication, producedplanktonic extracell protein extract (PEP) and biofilmextracell proteinextract (PEB), planktonic intracell protein extract (PIP), and biofilmintracell protein extract (PIB), withprotein content 1.44; 1.29; 1.29 and 1.21 mg/ml respectively. Thebiofilm intracell protein (BIP) showed antigenic property towardantibody anti-Candida (positive control),giving red spot on imunodot assay. Immunodot assay can distinguishnegative control serum (health man) and positive Candidiasis control by using antigen 1 mg/ml and50ml serum. Keywords: C. albicans, candidiasis, biofilm, immunodot assay
The Potency of Protein Extracts from Candida albicansas Bioreceptor on Immunosensor for Diagnosis of Candidiasis Masfufatun .; Noer Kumala; Afaf Baktir
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Currently diagnosis of candidiasis still usingthe traditional standard blood culture method. The traditional method were less sensitive andtime consuming. The purpose of this research were to develope the more sensitive immunosensor based method, and to examine the potency of C. albicans protein extract as bioreceptor to  detect  C. albicans and its biofilm in the blood of candidiasis patients.The research methods include: (1) preparation of  digestive gland liquid of snail (Achatina fulica); (2) extraction of protein from C. albicans  through  enzimatis and mechanic methodsand (3) analyzing the protein extract as bioreseptor through immunodot assay.The research results showed that the snail enzymes has protein content 1.35 mg/ml and  specific activity 1.96 unit/mg. The snail enzyme hydrolyzed  the cell wall of C. albicans  with and without sonication, producedplanktonic extracell protein extract (PEP) and biofilmextracell proteinextract (PEB), planktonic intracell protein extract (PIP), and biofilmintracell protein extract (PIB), withprotein content 1.44; 1.29; 1.29 and 1.21 mg/ml respectively. Thebiofilm intracell protein (BIP) showed antigenic property towardantibody anti-Candida (positive control),giving red spot on imunodot assay. Immunodot assay can distinguishnegative control serum (health man) and positive Candidiasis control by using antigen 1 mg/ml and50ml serum. Keywords: C. albicans, candidiasis, biofilm, immunodot assay
Co-Authors Abidah Ardilia AFAF BAKTIR Agusniar Furkani Listyawati akhmad sudibya, akhmad ALMAIDAH SAFITRI Amalia Nadiasari Ananta Sandi Putra Ananta, Zahrah Aswa Andini Risma Ayunda Dewi Andriani, Chenny Aritania, Putu Oky Asyamsa Hidayat, Rheina Faticha Atik Sri Wulandari Ayly Soekanto Ayu Cahyani Noviana Budhi Setiawan Budhi Setiawan Budhi Setyawan Chenny Andriani Wiyono Colin, Muhammad Dadik Raharjo Deddy Hartanto Diah Setijowati, Eva Dinara Safina Salsabillah Dwi Cahyani, Herawati Dyah Virga Pujiani Dzulfira Arifah Elika Joeniarti Emilia Devi D.R, Emilia Devi Farizah El Husna Fauzan, Laili Shafarina Febtarini Rahmawati Fehima Syachsiyat Alya Firdausi Nuzula Handy Arief HARMAN AGUSAPUTRA Harsono Harsono Haryanto, Loo Herliani, Olivia I Kadek Wawan Agus Wijaya I MADE DWIKY KARINA JAYA Inawati Inawati Inawati Inawati Inawati Inawati, Inawati Indahasari, Noer Kumala JAMES HADIPUTRA SUNARPO Jonathan Kartika Ishartadiati Kuntaman Kuntaman Kuntaman Kuntaman Kuntaman Kuntaman Laili Shafarina Fauzan Loo Hariyanto Raharjo, Loo Hariyanto Lusiani Tjandra Made Wirajaya Kesuma Marshanda Venda Dora Putri Aziz Masfufatun Meilya Putri Syahida Mohammad Ali Izzudin Mokhtar, Siti Aisyah Muhammad Rozzan Prananda Nadila, Sucia Narotama, Harya Ni Luh Narita Vijayanti Ni Made Putri Pradnyandari NI PUTU MANIK SURYANINGSIH Noer Kumala Noer Kumala NOER KUMALA Noer Kumala Indahsari, Noer Kumala Novina Aryanti Nugraha, Wima Handika NUGROHO EKO WIRAWAN BUDIANTO Nur Kumala Indahsari, Nur Kumala Nur Kumala, Nur Nurma Fitri Nurul Izzah Parawidnyaningsih, Putu Ayu Aryanda Pramita Laksitarahmi Isrianto Pratika Yuhyi Hernanda Putu Oky Ari Tania Rachmawati Dwi Apriliyani Ratnasari, Diana Tri Rianti, Emilia Devi Dwi Rini Purbowati Rini Purbowati Riska Ammalia Putri Risnanda, Kadek Rio Sakri, Brian Manggala Setijowati, Eva Diah Siswahyudianto Sri Lestari Utami Sugiharto Suhartati Suhartati Suhartati Syahrefi Syahrefi TEOFILUS DANI PRASETYOADI Tikapa Muwawiyai Pigome Tri Juliati Tri Rahayuningsih Tri Ratnasari, Diana Utami, Sri Lestari Vini Anggraini Wahyuni Dyah Parmasari, Wahyuni Dyah Wiyono, Chenny A Yaniari, Roethmia