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Formulation and Physicochemical Characterization of a Macroemulsion Containing Ethanolic Extract of Kemuning Leaves (Murraya paniculata (L.) Jack) Weningtyas, Anditri; Wiratama, Arif Ladika; Wibisono, Taurisma Aulia Nanda; Septiananda, Farsya Hidayah; Prastita, Nenes
BIOEDUSCIENCE Vol 10 No 1 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/20395

Abstract

Background: Ethanolic extract of Murraya paniculata leaves contains several bioactive constituents, but its direct application is limited by physicochemical instability and solubility issues. Objective: This study aimed to formulate and characterize macroemulsions containing ethanolic extract of Murraya paniculata leaves and to identify the most promising formulation based on physicochemical evaluation.   Methods: The extract was prepared by maceration using 96% ethanol. Four macroemulsion formulations were developed using different ratios of virgin coconut oil, Tween 80, PEG 400, and water. The formulations were evaluated through phytochemical screening, organoleptic properties, emulsion type, transmittance, particle size distribution, pH, zeta potential, centrifugation, and cycling test.   Results: The extract contained flavonoids, alkaloids, tannins/phenolics, and triterpenoids. All formulations were identified as oil-in-water emulsions. Based on the reported data, F2 showed high transmittance and a low polydispersity index, while F1 and F2 demonstrated better physical stability than F3 and F4.   Conclusions: The study indicates that F1 and F2 were the most promising formulations; however, the claim of a single best formulation should be supported by complete, consistently presented particle-size, zeta-potential, and stability data.
Nutritional Determinants and Molecular Insights into Anemia among Boarding School Adolescents Kiky Martha Ariesaka; Lintang Widya Sishartami; Anditri Weningtyas; Moh. Mirza Nuryady
Jurnal Kesehatan Masyarakat Vol. 21 No. 3 (2026)
Publisher : Universitas Negeri Semarang in collaboration with Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI Tingkat Pusat) and Jejaring Nasional Pendidikan Kesehatan (JNPK)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v21i3.35386

Abstract

Introduction: Anemia remains a significant public health problem among adolescents in developing countries such as Indonesia. Female adolescents are more vulnerable due to menstrual blood loss and increased iron needs during growth, while dietary diversity and beverage habits may further influence anemia risk. Aim: This study aimed to analyze factors associated with anemia, including gender, diet quality, and tea or coffee consumption, and to explore their molecular relevance using a bioinformatics approach. Methods: A cross-sectional study was conducted among 199 adolescents (105 males, 94 females) aged 12–17 years in a boarding school in Malang, Indonesia. Data collection included hemoglobin measurement, anthropometric assessment, and a Food Frequency Questionnaire (FFQ). Statistical analysis was performed using Chi-square and logistic regression, while bioinformatics analysis employed STRING, Cytoscape, and ShinyGO to identify key genes and pathways. Results: Female gender (AOR = 6.595; p < 0.001), adequate diet (AOR = 0.184; p = 0.012), and tea/coffee intake (AOR = 7.532; p < 0.001) were significant predictors of anemia. Bioinformatics analysis identified SLC11A2, TFRC, and HAMP as key genes regulating ferroptosis and mineral absorption pathways. Conclusions: Gender, dietary quality, and tea or coffee consumption significantly influence anemia in adolescents.