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Kinetika Hidrolisis Selulosa Menjadi Glukosa Pada Limbah Kulit Nanas (Ananas comosus) Menggunakan Enzim Selulase Annidya, Putri Azmi; Rachmanto, Tuhu Agung; Novembrianto, Rizka
Journal of Natural Sciences Vol 6, No 3 (2025): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v6i3.1033

Abstract

Pineapple peel waste (Ananas comosus) is a source of organic waste with high lignocellulose content that has the potential to be utilized through a bioconversion process into glucose. This study aims to determine the Michaelis–Menten kinetic equation of cellulose hydrolysis into glucose using cellulase enzymes and analyze the effect of substrate concentration (1–5% w/v) and hydrolysis time (60–180 minutes) on the reaction rate. The research stages include delignification using 5% NaOH at 80ºC and enzymatic hydrolysis with 10% cellulase at pH 5 and 50ºC. Glucose levels were measured using a Brix refractometer through a standard glucose solution calibration method. The results showed an increase in glucose concentration over time and substrate concentration, with the highest conversion of 91.5% at 5% substrate. Kinetic analysis using the Michaelis–Menten equation yielded an average reaction rate constant (k) of 0.3328 min⁻¹, with the highest k of 0.3828 min⁻¹ and the highest Michaelis–Menten constant ( ) of 2.357 mg·L⁻¹ at a substrate concentration of 5%. A significant increase in  from 0.2403 (1%) to 2.357 (5%) indicates a decrease in enzyme affinity due to substrate saturation. Therefore, a concentration of 5% is considered the optimal condition for the cellulose hydrolysis process, confirming the potential of pineapple peel waste as an efficient substrate source.
Implementasi Instalasi Pengolahan Air Limbah (IPAL) Untuk Mengatasi Pencemaran di Kantin Warung NKRI UPN “Veteran” Jawa Timur Nisa, Diana Aqidatun; Novembrianto, Rizka; Gunawan, Rizal; Shanty, Dina Kurnia; Aulia, Salsabila
Jurnal Sains Teknologi dalam Pemberdayaan Masyarakat Vol. 6 No. 2 (2025): Desember 2025
Publisher : Fakultas Teknik Universitas Bhayangkara Jakarta Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31599/2bvpgk93

Abstract

Universitas Pembangunan Nasional "Veteran" Jawa Timur (UPNVJT) is committed to realizing a sustainable campus. However, this commitment was challenged at the Warung NKRI canteen, where liquid waste was discharged into open sewers, causing pollution and odor. This Community Service Program aimed to solve this issue through a multidisciplinary approach. The methods included educating vendors and customers on waste management and implementing a Wastewater Treatment Plant (WWTP/IPAL). To ensure sustainability, socialization on system maintenance was conducted for the Business Management Unit (BPU) and cleaning service staff. The results showed increased awareness within the canteen community and the WWTP's success in producing clearer, odorless effluent. This program proves that cross-disciplinary collaboration can deliver tangible and sustainable solutions for campus environmental sanitation.
KINETIKA REAKSI PERTUMBUHAN BAKTERI PSEUDOMONAS FLUORESCENS PADA PROSES FERMENTASI AIR LINDI Ghany Firmansyah; Tuhu Agung Rachmanto; Rizka Novembrianto
BIOLOVA Vol. 7 No. 1 (2026): Volume 7 Nomor 1 Maret Tahun 2026
Publisher : Universitas Muhammadiyah Metro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24127/biolova.v7i1.10548

Abstract

Abstrak: Air lindi merupakan limbah cair hasil dekomposisi sampah di Tempat Pemrosesan Akhir (TPA) yang berpotensi mencemari lingkungan karena kandungan bahan organik dan logam beratnya yang tinggi. Penelitian ini bertujuan untuk menganalisis kinetika pertumbuhan bakteri Pseudomonas fluorescens serta karakteristik fisik hasil fermentasi air lindi. Proses fermentasi dilakukan secara aerob selama 8 hari dengan variasi volume inokulum bakteri 5 mL, 7 mL, dan 10 mL dalam 100 mL air lindi. Parameter yang diamati meliputi pH, suhu, konsentrasi C-organik, dan jumlah koloni bakteri. Hasil menunjukkan peningkatan pertumbuhan bakteri secara signifikan pada fase logaritmik dengan pola linear hubungan antara ln(CA0/CA) dan ln(CC/CC0). Nilai koefisien determinasi (R²) mendekati 1, yang mengindikasikan kesesuaian dengan model kinetika pertumbuhan. Selain itu, terjadi penurunan kandungan C-organik dan logam berat, serta perubahan karakteristik fisik air lindi yang menunjukkan peningkatan kualitas hasil fermentasi. Dengan demikian, fermentasi air lindi menggunakan Pseudomonas fluorescens berpotensi mengurangi dampak pencemaran dan meningkatkan nilai guna air lindi sebagai hasil olahan ramah lingkungan. Abstract: Leachate is a liquid waste generated from the decomposition of solid waste in landfill sites, which has the potential to pollute the environment due to its high content of organic matter and heavy metals. This study aims to analyze the growth kinetics of Pseudomonas fluorescens and the physical characteristics of leachate after the fermentation process. The fermentation was carried out aerobically for 8 days with variations of bacterial inoculum volumes of 5 mL, 7 mL, and 10 mL in 100 mL of leachate. The observed parameters included pH, temperature, C-organic concentration, and bacterial colony count. The results showed a significant increase in bacterial growth during the logarithmic phase, following a linear relationship between ln(CA0/CA) and ln(CC/CC0). The determination coefficient (R²) values were close to 1, indicating good conformity with the microbial growth kinetics model. In addition, there was a reduction in C-organic and heavy metal contents, as well as changes in the physical characteristics of the leachate, indicating improved quality of the fermentation product. Therefore, leachate fermentation using Pseudomonas fluorescens has the potential to reduce environmental pollution and enhance the usability of leachate as an environmentally friendly processed product.
Analysis of Aluminum Sulfate Dosage on Water Quality Parameters in the Coagulation-Flocculation Process: Analisis Dosis Aluminium Sulfat terhadap Parameter Kualitas Air Pada Proses Koagulasi-Flokulasi Fadhilah Labibah Nurjanah; Okik Hendriyanto Cahyonugroho; Rizka Novembrianto
Media Ilmiah Teknik Lingkungan (MITL) Vol. 11 No. 1 (2026): Media Ilmiah Teknik Lingkungan (MITL)
Publisher : ​Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/mitl.v11i1.11181

Abstract

This study aims to evaluate the performance of aluminum sulfate (alum) coagulant in river water, tofu waste, and batik waste with varying doses on the effectiveness of the coagulation-flocculation process. The method used was a jar test on 1000 mL of water samples, including coagulation for 1 minute at 120 rpm, and flocculation for 20 minutes at 40 rpm, and sedimentation for 15 minutes. The variations in alum doses used were 150 mg/L, 200 mg/L, and 250 mg/L. The results showed that this process caused pH changes during treatment and was effective in reducing turbidity, TSS, and color although the optimum dose differed for each sample. In river water, the optimum dose of 150 mg/L resulted in 98.99% turbidity removal, 81.82% TSS removal, and 99.11% color removal. Meanwhile, for tofu waste, the optimum dose of 250 mg/L resulted in 86.67% turbidity removal, 98.66% TSS removal, and 96.16% color removal. Meanwhile, for batik waste, the optimum dose of 200 mg/L resulted in 97.89% turbidity removal, 95.76% TSS removal, and 91.61% color removal. This indicates that the coagulant dosage needs to be adjusted to the characteristics of each type of sample used.
Co-Authors Abdus Salam Jawwad, Muhammad Adisty Tamandita Agustini, Rika Yayu Ali, Munawar Ali, Munawar Ambarsari, Utami Andini, Ari Annidya, Putri Azmi Aulia, Salsabila Ayu Murti, Restu Hikmah Azizah Mahirah Rizki Azzahra, Maulayya Fatimah Bella Meitha Wulandari Bella Meitha Wulandari Dea Febrica Ervina Denaya Andrya Prasidya Dewi Intan Elok Mayangsari Diana Aqidatun Nisa Dwi Setiyaningtyas Early Agista Mahardhika Ervina, Dea Febrica Erwin Kurniawati Euis Nurul Hidayah Fadhilah Labibah Nurjanah Firdaus, Nisa Afyfh Firra Rosariawari Ghany Firmansyah Gina Lova Sari Gunawan, Rizal Hakam, Muhammad Harmoko, Dian Fitri Hikmah Ayu M, Restu Hutapea, Octavianus Hutapea Ibnu Sholichin Ignacia Putri Dinayah Isna Nugraha Jawwad, Muhammad Abdus Salam Kholifah, Wilujeng Rastinur Kindriari Nurma Wahyusi Lavyatra, Dinda Ayu M N Rhomadhoni Mar'atus Sholikhah Maulayya Fatimah Azzahra Mirwan, Mohamad Mohamad Mirwan Mohamad Sholikin Muhammad Abdus Salam Jawwad Muhammad Riza Pahlevi Munawar Munawar Munawar Munawar Muslikha Nourma Rhomadhoni Muslikha Nourma Rhomadhoni Navisa Ika Irmayanti Nisa Afyfh Firdaus Nisa', Syadzadhiya Qothrunada Zakiyayasin Nisa', Syadzadhiya Qotrunada Zakiyayasin Nisa, Syadzadhiya Qothrunada Zakiyayasin Nugroho, Adelia Nurkhalisha, Seren Okik Hendriyanto Cahyonugroho Okik Hendriyanto Cahyonugroho Okik Hendriyanto Cahyonugroho Oris Hatpa Aditya Azhar Pandu Wicaksono Pandu Wicaksono Permana, Endah Budi Praditya Sigit Ardisty Sitogasa Pramesti , Hafidya Norista Pranandito Wisnu Prabowo Putri Redita Rositasari Putri Redita Rositasari Putri, Marsanda Amelia Q Z N, Syadzadhiya Qatrunada, Sekar Huwaidah R H A Murti Rachmanto, Tuhu Agung Raden Kokoh Haryo Putro Ramadhani, Audyna Ayu Restu Hikmah Ayu Murti Rhomadhoni, Muslikha Nourma Rizki, Azizah Mahirah Rosariawari, Firra Rositasari, Putri Redita Safitri, Annisa Nur Shanty, Dina Kurnia Sholikhah, Mar'atus Sholikin, Mohamad Tuhu Agung Rachmanto W D Lestari Wahyudi, Muhammad Ilham Wang, Ya-Fen Wulandari, Bella Meitha Wulandari, Salsabila Puspita You, Shen-Jie Zain, Alifia Salsabila