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All Journal Journal of Food and Pharmaceutical Science JURNAL GIZI INDONESIA Media Kesehatan Masyarakat Indonesia Media Gizi Mikro Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) agriTECH Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Paediatrica Indonesiana GIZI INDONESIA Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) JNKI (Jurnal Ners dan Kebidanan Indonesia) (Indonesian Journal of Nursing and Midwifery) Jurnal Gizi Klinik Indonesia Journal of Community Empowerment for Health Jurnal Dunia Gizi Narra J JURNAL KESEHATAN SAMODRA ILMU Berita Kedokteran Masyarakat Proceeding ISETH (International Summit on Science, Technology, and Humanity) Journal of Food and Pharmaceutical Sciences
Emy Huriyati
Department Of Health Nutrition, Faculty Of Medicine, Public Health, And Nursing, Universitas Gadjah Mada, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Education Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Journal : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

 1 
Correlation of methylation of toll-like receptor 4 (TLR4) and interleukin-6 (IL6) promoter with insulin resistance in obese adolescents Ratih Feraritra Danu Atmaja; Dian Caturini Sulistyonigrum; Emy Huriyati; Ahmad Hamim Sadewa; Rina Susilowati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 1 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1035.405 KB) | DOI: 10.19106/JMedSci004801201602

Abstract

Chronic inflammation can lead to insulin resistance in obesity. Increased mRNA expressionof inflammatory markers such as toll-like receptor 4 (TLR4) and interleukin 6 (IL6) werereported both in the tissue and the peripheral blood of obese adolescent and correlatedwith insulin resistance. DNA methylation surrounding TSS region is known to regulate thelevel of a gene expression. The aim of the study was to investigate the methylation statusof TLR4 and IL6 promoter in peripheral blood of obese adolescent and its correlation toinsulin resistance. This was a cross sectional study with observational analytic approached.Fifty adolescents with obesity from Yogyakarta Special Region, aged 15-18 years,z-score value >2 SD, no experience of any acute infections within 2 weeks and signed ininformed consent, were selected in this study. Subjects with diabetic mellitus, incompletedata and DNA concentration of <20 μg/mL were excluded. DNA samples were extractedfrom peripheral blood of the subjects. The bisulfite converted DNA was analyzed formethylation level with direct sequencing. Correlation analysis using Spearman test wasperformed with significance value was set at p<0.05. No methylation in TLR4 promoterwas detected in all subjects. The methylation level of IL6 was transformed to categoricalvariable. Four (8%) subjects with insulin resistance and 2 (4%) without insulin resistancehad methylation on a CpG site. Methylation of IL6 was not statisticaly different betweeninsulin resistance and non-resistance adolescent (p=0.635). The significant correlationwas not found between methylation level in IL6 promoter with insulin resistance (HOMAIR)(r=0.051). Cell specific of methylation mechanism, characteristic of subjects andtypes of samples were suspected causing those results. In conclusion, no correlationbetween methylation levels of TLR4 promoter and IL6 with insulin resistance in obeseadolescent was observed in this study.
Co-Authors A’yun, Rizka Qurrota Abdul Wahab Abeng, Andi Tenri Abidillah Mursyid Adhila Fayasari Ahmad Hamim Sadewa Ailsa Nur Rahma Apta Widyadhana Akhmad Sujai Almira Nadia Alpha Olivia Hidayati Andi Tenri Abeng Annisa Leny Saraswati Arta Farmawati Atika Sulistyan Ave Anugraheni Cati Martiyana Christin Debora Nabuasa Dangga, Victorianus Daniel Robert Daniel Robert, Daniel Darwin Nasution Detty Siti Nurdiati Dewi Marfuah Dian Caturini Sulistyonigrum Djauhar Ismail Djauhar Ismail Djauhar Ismail Djauhar Ismail Dwi Sarbini Dyah Ayu Kusuma Wardani Elisabeth Pampang Eni Harmayani Eni Harmayani Erinda Nurrahma Fajar Sri Tanjung Farida Nailufar Fatwa Sari Tetra Dewi Fitri Ardiani Fitri Ardiani Gebang, Antonius Adolf Hamam Hadi Hamam Hadi Hamam Hadi Hamam Hadi Happy Nurlita Octavinanda Hardiana Probosiwi Hari Kusnanto Harry Freitag Luglio Muhammad Hidayah, Rizki Amalia Husnah Husnah Inas Nur Hafizhah Janatin Hastuti Kamsiah . Kantari, Eka Suci Khristina Dias Utami Lastiana Safitri Lastiana Safitri, Lastiana Latifah Mahdiyati Lely Lusmilasari, Lely Lily Arsanti Lestari Lisdrianto Hanindriyo Lisma Evareny, Mohammad Hakimi, Retna Siwi Padmawati M Juffrie Madarina Julia Madarina Julia Madarina Julia Madarina Julia Mae Sri Hartati Wahyuningsih Maria Goretti Adiyanti Maria Goretti Adiyanti, Maria Goretti Martalena Br Purba Martalena Br Purba Martalena Br Purba Martalena Br Purba, Martalena Br Mirza Hapsari Sakti Titis Penggalih Mirza Hapsari Sakti Titis Penggalih, Mirza Hapsari Sakti Titis Mubasysyir Hasanbasri Mubasysyir Hasanbasri Muhammad Jourdan Prasetyatama Mursyid, Abidillah Mustikaningtyas Mustikaningtyas Mustikaningtyas, Mustikaningtyas Mutiara Tirta Prabandari Lintang Kusuma Nailufar, Farida Nasution, Darwin Ni Komang Wiardani Ningrum, Ema W. Nisa, Ainun Nur Fitri Widya Astuti Nurahma, Bira Arumndari Okta Haksaica Sulistyo Pampang, Elisabeth Perdana Samekto Tyasnugroho Suyoto Podojoyo Podojoyo Podojoyo, Podojoyo RAHMANIAH RAHMANIAH Ramadhaniah Ramadhaniah Ramadhaniah, Ramadhaniah Ratih Feraritra Danu Atmaja Refdiana Dewi Ribia Tutstsintaiyn Rina Susilowati Rina Susilowati Rissa Saputri Rizka Qurrota A’yun Ronny Tri Wirasto Rupiasa, Welresna Juliatri Putri Sadewa, Hamim Saputri, Rissa Siti Wahdah Sri Ahadi Nugraheni Sri Ahadi Nugraheni, Sri Ahadi Sri Rahayoe Sujai, Akhmad Sulistyan, Atika Sulistyo, Okta Haksaica Sunarti, Sunarti Supriyati Susetyowati Susilo, Leonardus Reynald Tiara Marthias Toto Sudargo Toto Sudargo Usi Lanita Utami, Nurul Putrie Wardani, Dyah Ayu Kusuma Winda Irwanti Winda Irwanti, Winda Wirasto, Ronny Tri Wiryatun Lestariana Wiryatun Lestariana Wiryatun Lestariana, Wiryatun Yayi Suryo Prabandari Yusmiati Yusmiati Yusmiati Yusmiati, Yusmiati Zahra Anggita Pratiwi