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All Journal Jurnal Pendidikan Biologi Undiksha Biotropika Jurnal Sumberdaya Hayati Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ilmiah Kesehatan Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Jurnal Abdimas Berdaya : Jurnal Pembelajaran, Pemberdayaan dan Pengabdian Masyarakat Jurnal Biosense Quagga: Jurnal Pendidikan dan Biologi Bagimu Negeri : Jurnal Pengabdian Kepada Masyarakat Maximus: Journal of Biological and Life Sciences Indonesian Journal of Jamu
Iffa Afiqa Khairani
Institut Teknologi Sumatera
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Antioxidant Activity and Immunomodulatory Effect of Stachytarpheta sp. Leaf Extract on Phagocytic Activity of Mice Peritoneal Macrophages in Alloxan Induction Iffa Afiqa Khairani; Wahyuni Dian Lestari; Elisa Nurma Riana; Jeane Siswitasari Mulyana; Hida Arliani Nur Anisa; Rizka Fatriani; Silvia Andriani
Quagga: Jurnal Pendidikan dan Biologi Vol 17 No 2 (2025): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v17i2.404

Abstract

Hyperglycemia is a condition characterized by elevated blood glucose levels, which is a hallmark of diabetes mellitus. Prolonged hyperglycemia not only disrupts glucose homeostasis but also impairs the immune response. The condition of hyperglycemia in the body can increase free radicals and oxidative stress, thus can impair various macrophage functions, including their ability to engulf and eliminate pathogens. The bioactive constituents in Stachytarpheta sp. may contribute to reducing oxidative damage and enhancing immune system function. This study aims to evaluate both the antioxidant capacity and the immunomodulatory effects of Stachytarpheta sp. leaf extract on the phagocytic activity of peritoneal macrophages in alloxan-induced diabetic mice. Alloxan is widely used in research to induce hyperglycemia (diabetic conditions) in experimental animals. The antioxidant activity of the extract was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay, while phagocytic activity of peritoneal macrophages was performed in vivo using male mice. Stachytarpheta sp. leaf extract from Beteng Sari Village, East Lampung has very strong antioxidant activity against DPPH with IC50 value 14,85 ± 0,30 μg/mL. This extract at doses of 200, 300, and 400 mg/kgBW can be an immunomodulator that maintains the phagocytic activity of macrophage cells in alloxan-induced mice.
Dampak Kualitas Tidur terhadap Sindrom Pramenstruasi pada Perempuan di Tiga Kecamatan Kabupaten Agam, Sumatera Barat Rara Mulia Sari; Winati Nurhayu; Iffa Afiqa Khairani; Andy Darmawan
Jurnal Penelitian Pendidikan IPA Vol 11 No 12 (2025): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i12.13021

Abstract

Premenstrual Syndrome (PMS) significantly impacts reproductive-age females, yet the interplay of lifestyle factors in rural contexts remains under-researched. This study aimed to analyze the relationship between sleep quality and PMS severity among females in Agam Regency, West Sumatra. A quantitative survey was conducted with 124 participants selected via purposive sampling. Data were collected using the Pittsburgh Sleep Quality Index (PSQI) and the Premenstrual Symptoms Screening Tool (PSST), then analyzed using Linear Models (LM) in R. Results showed that 84.67% of respondents reported poor sleep quality. LM analysis revealed that poor sleep quality significantly predicted severity across all PMS categories: PMDD (p = 0.0054), psychological/somatic symptoms (p = 0.0013), and daily dysfunction (p = 0.0007). Additionally, higher BMI was linked to increased PMDD symptoms (p = 0.0290), while stress significantly influenced daily functional impairment (p = 0.0234). In conclusion, poor sleep quality is a primary predictor of severe PMS, exacerbated by high BMI and stress. These findings suggest that sleep hygiene and stress management are critical non-pharmacological interventions for improving menstrual health in rural populations.
Integrated Metabolite Profiling and Network Pharmacology Reveal the Multitarget Therapeutic Potential of Curcuma amada Rhizome Fatriani, Rizka; Istiadi, Khaerunissa Anbar; Khairani, Iffa Afiqa; Anisa, Hida Arliani Nur
Biotropika: Journal of Tropical Biology Vol. 14 No. 1 (2026)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/

Abstract

Curcuma amada Roxb. (mango ginger) is a medicinal plant traditionally known for its antioxidant, anti-inflammatory, and anticancer effects, however, the underlying molecular mechanisms are not fully understood. In this study, we combined metabolite profiling with in silico analysis (network pharmacology and molecular docking) to understand the bioactivity of C. amada. Gas chromatography-mass spectrometry (GC-MS) revealed a diverse metabolite composition with fifty-two compounds detected in the ethanol extract. Target prediction of the compounds was performed using SwissTargetPrediction, BindingDB, and ChEMBL. Hub genes were identified using Maximum Clique Centrality (MCC), which identified ESR1, PPARG, and NR3C1 as the main hub genes. Enrichment analysis showed a strong association with cancer-related pathways, particularly the PI3K–Akt signalling, endocrine resistance, and cell senescence pathways, as well as the biological processes regulating apoptosis, stress response, and intracellular signalling. Molecular docking studies of PPARG and ESR1 showed that the metabolites retinal, coronarin E, and 1H-cycloprop[e]azulene derivatives had stable binding affinities and interacted with residues in the ligand-binding domain (LBD) of the target proteins. Our findings suggest that C. amada exerts therapeutic effects through multiple mechanisms. Not a single molecule or pathway, but a coordinated network of metabolites acting on multiple targets. This integrative approach provides a scientific basis for the traditional use of C. amada and highlights its potential as a promising resource for drug discovery, particularly in the field of cancer therapy.
Perbedaan Profil Protein Plasma Darah Mencit Menggunakan SDS-PAGE Tanpa Penambahan dan dengan Penambahan 2-Mercaptoethanol Khaerunissa Anbar Istiadi; Iffa Afiqa Khairani; Silvia Andriani
Jurnal Pendidikan Biologi Undiksha Vol. 11 No. 2 (2024)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v11i2.82139

Abstract

SDS-PAGE is a method used to analyze proteins based on differences in molecular weight. Sample preparation in SDS-PAGE with 2-mercaptoethanol will reduce disulfide bonds in proteins. The sulfide bond reduction process will cause differences in protein conformation and profile. The purpose of this study was to compare the pattern of blood plasma protein profiles of mice analyzed using SDS-PAGE, with the addition of 2-mercaptoethanol and without the addition of 2-mercaptoethanol in a mixture of sample buffer solutions. The results of SDS-PAGE showed that the blood plasma protein profile of mice without the addition and with the addition of 2-mercaptoethanol showed differences in the number of protein bands, the molecular weight of several proteins and the thickness of the protein bands. There were nine protein bands with a molecular weight above 52 kDa in the profile without the addition of 2-mercaptoethanol, while there were only five protein bands with a molecular weight above 52 kDa in the profile with the addition of 2-mercaptoethanol. The difference in protein profile patterns shows that mouse blood plasma contains proteins containing disulfide bonds that can be reduced by 2-mercaptoethanol.
Phytochemical Compound, Antioxidant and Antibacterial Properties of Sargassum sp. Extracts from Pahawang Island, Lampung Province Iffa Afiqa Khairani; Khaerunissa Anbar Istiadi; Nawang Restiawati
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.6558

Abstract

Pahawang Island, located in Lampung Bay, has a variety of potential natural resources, one of which is macroalgae. One of the abundant brown macroalgae is Sargassum sp., with phytochemical components (flavonoids, steroids, tannins, and glycosides) that have potential as antioxidants, antibacterial, antitumor, anticancer, antifouling, and others. Sargassum sp. from Lampung waters, especially Pahawang Island still lacks scientific studies on its bioactive content and properties. This study aims to examine the phytochemical compounds (qualitatively and quantitatively), antioxidant activity (DPPH method) and antibacterial potential of Sargassum sp. extract (MIC/MBC method) against Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The results of phytochemical assay  showed that Sargassum sp. extract contained phenolic compounds, flavonoids, tannins, and saponins. Quantification results on total phenolic content were 0.56 mgGAE/g extract, total tannins were 4.27 mgTAE/g extract, total flavonoids were 14.19 mgQE/g extract, and total saponins were 5.54 %. Sargassum sp. extract classified as a very weak antioxidant with IC50 value of 964.10 ppm, and moderate antibacterial activity at a concentration of MIC/MBC 250/250 ppm against B. subtilis, E. coli, P. aeruginosa, and S. aureus
EFEK EKSTRAK KULIT BUAH KABAU (Archidendron bubalinum (Jack) I.C Nielsen) TERHADAP AKTIVITAS MAKROFAG MENCIT JANTAN SETELAH DIPAPARKAN CdCl2 Elisa Nurma Riana; Iffa Afiqa Khairani; Hida Arliani Nur Anisa; Rizka Fatriani; Silvia Andriani
JURNAL BIOSENSE Vol 9 No 2 (2026): Edisi April 2026
Publisher : Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i2.7817

Abstract

Logam kadmium (Cd) yang berasal dari kegiatan industri seperti produksi baterai, pelapisan logam, penggunaan pupuk fosfat, pembakaran bahan bakar fosil, dan pembuatan plastik dapat meningkatkan produksi reactive oxygen species (ROS). Produksi ROS berlebih dalam tubuh akan menyebabkan penyakit dan mempengaruhi sistem imun tubuh akibat stres oksidatif. Stres oksidatif merupakan suatu kondisi ketidakseimbangan antara radikal bebas dan antioksidan. Untuk melindungi tubuh dari stres oksidatif diperlukan suatu senyawa antioksidan, seperti yang terkandung dalam kulit buah kabau (Archidendron bubalinum). Penelitian ini bertujuan mengetahui mengetahui adanya peningkatan aktivitas fagositosis makrofag pada mencit Jantan yang dipaparkan CdCl2. Penelitian ini bersifat eksperimental menggunakan rancangan acak lengkap (RAL). Mencit yang digunakan 24 ekor dengan 6 kelompok yang terdiri dari 3 kelompok kontrol dan 3 kelompok perlakuan. Hasil pengujian menunjukkan bahwa pemberian ekstrak kulit buah kabau dosis 95 mg/kgBB, 190 mg/kgBB dan 380 mg/kgBB pada uji aktivitas fagositosis makrofag menunjukkan hasil yang signifikan sebesar p = 0,005. Hal ini berarti bahwa ekstrak kulit buah kabau dapat meningkatkan sistem imun mencit dengan perlakuan P2 memiliki aktivitas fagositosis tertinggi yaitu 55,91%.
Co-Authors Anbar Istiadi, Khaerunissa Andri Jaya Kesuma Andriani, Silvia Andriyana, Ahmad Dodi ANDY DARMAWAN Andy Darmawan Anisa, Hida Arliani Nur Anshori, Andi Antonius Antonius Arliani Nur Anisa, Hida Azizah, Najla Nur Dewi Chusniasih Evi Kurniawaty Fatriani, Rizka Gres Maretta, Gres Haryandi, Yopi Hendri Busman Hida Arliani Nur Anisa Hida Arliani Nur Anisa Jeane Siswitasari Mulyana Khaerunissa Anbar Istiadi Khaerunissa Anbar Istiadi Kurnia Wahyuni Kurniawaty, Evi Leksikowati, Sovia Santi Maruli, Berkat Afni Mulyadi, Salsa Pratiwi Mulyana, Jeane Siswitasari Nanda, M. Zulhiyadi Natalia Pardede, Desta Nawang Restiawati Novriadi, Novriadi Nuning Nurcahyani Nurhayu, Winati Olivia, Rahel Noviantika Puspa, Egita Windrianatama Putri, Isnaini Anggraini Rara Mulia Sari Riana, Elisa Nurma Rizka Fatriani Sahroni, Mizan Sandro, Mario Silvia Andriani Silvia Andriani Siswahyudianto Suryanti, Erma Wahyuni Dian Lestari Wandasari, Septi Putri Winati Nurhayu Yanti Ariyanti, Yanti Ariyanti Yogi Catur Putra