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All Journal ODONTO Dental Journal Majalah Obat Tradisional Dentino: Jurnal Kedokteran Gigi Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Journal of Indonesian Dental Association Jurnal Kreativitas PKM Dentin Jurnal Insan Farmasi Indonesia Odonto dental journal
Tri Putri, Deby Kania
Department Of Biomedic, Faculty Of Dentistry, Universitas Lambung Mangkurat, Banjarmasin
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Physics Public Health

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Journal : Dentin

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UJI TOKSISITAS KITOSAN SISIK IKAN HARUAN (Channa striata) TERHADAP SEL VERO Azwar Fida Maulana; Deby Kania Tri Putri; Aulia Azizah
Dentin Vol 6, No 2 (2022)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v6i2.6390

Abstract

ABSTRACTBackground: Periodontal disease is one of the oral disease problems that still need attention. These diseases are among the diseases with the second highest number of sufferers after caries. Periodontitis is a multifactorial that causes inflammation of the periodontal tissue. Periodontitis is caused by bacteria that trigger infectious diseases in the supporting tissues of the teeth. Haruan fish scale chitosan has potential as an antimicrobial, because it contains lysozyme enzymes and aminopolysacharida groups that can inhibit microbial growth. Haruan fish scale chitosan in suppressing bacterial growth is due to its positively charged polycation. Objective: To analyze whether haruan fish scale chitosan is toxic at concentrations of 20%, 40%, 75% and 100% against vero cells. Methods: This study used a true experimental type with a post-test-only design with a control group design to test the toxicity of chitosan do vero cell with concentrations 20%, 40%, 75% and 100% using MTT assay method. The study used 4 treatment groups and 2 control groups with 4 repetitions. Data analyzed with probit analyisis. Results: The largest cell viability values were concentrations of 100% and 75% of 100%, 40% of 81.4% and 20% concentration of 68.1%. Chitosan with concentrations of 20%, 40%, 75% and 100% was said to be non-toxic due to cell viability 60%. Value IC50 93103,354 μg/mL > 1000 μg/mL categorized non-toxic. Conclusion: Haruan fish scale chitosan with concentrations of 20%, 40%, 75% and 100% against vero was tested to vero cells was not have toxic effect. Keywords: Chitosan, Toxicity, Vero cells. ABSTRAKLatar Belakang: Penyakit periodontal termasuk penyakit dengan penderita tertinggi kedua setelah karies. Periodontitis merupakan penyakit peradangan jaringan periodontal. Periodontitis disebabkan oleh bakteri pemicu infeksi pada jaringan penyangga gigi. Kitosan sisik ikan haruan memilki potensi sebagai antimikroba, karena mengandung enzim lysosim dan gugus aminopolysacharida yang dapat menghambat pertumbuhan mikroba. Kitosan sisik ikan haruan menekan pertumbuhan bakteri karena memiliki polikation bermuatan positif. Tujuan: Menganalisis apakah kitosan sisik ikan haruan bersifat toksik pada konsentrasi 20%, 40%, 75% dan 100% terhadap sel vero. Metode: Penelitian ini menggunakan jenis true experimental dengan rancangan post test-only with control group design untuk menguji toksisitas kitosan sisik ikan haruan terhadap sel vero demam konsentrasi 20%, 40%, 75% dan 100% dengan metode MTT assay. Penelitian menggunakan 4 kelompok perlakuan dan 2 kelompok kontrol dengan 4 pengulangan. Data dianalisis menggunakan analisis probit. Hasil: Nilai viabilitas sel terbesar adalah konsentrasi 100% dan 75% sebesar 100%, 40% sebesar 81,4% dan konsentrasi 20% sebesar 68,1%. Kitosan dengan konsentrasi 20%, 40%, 75% dan 100% dikatakan tidak toksik dikarenakan viabilitas sel ≥ 60%. Nilai IC50 93103,354 μg/mL > 1000 μg/mL termasuk kategori tidak toksik. Kesimpulan: Kitosan sisik ikan haruan dengan konsentrasi 20%, 40%, 75% dan 100% yang diujikan terhadap sel vero tidak memiliki sifat toksik.  Kata kunci: Kitosan, Sel Vero, Toksisitas.
EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN RAMBAI (Sonneratia caseolaris) TERHADAP PERTUMBUHAN BAKTERI Aggregatibacter actinomycetemcomitans Melati Ainun Nisa; Beta Widya Oktiani; Deby Kania Tri Putri
Dentin Vol 6, No 3 (2022)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v6i3.6823

Abstract

ABSTRACTBackground: Aggressive periodontitis is a disease that destroys periodontal tissue rapidly caused by the dominance of the bacterium Aggregatibacter actinomycetemcomitans by 90%. Aggressive periodontitis treatment may include antibiotics. One of the antibiotics that can be used is metronidazole, but this metronidazole can have side effects when used in the long term such as headaches, nausea, dry mouth, and a metallic taste in the mouth. There are herbal plants, namely rambai leaves (Sonneratia caseolaris) which contain compounds that can be used as antibacterials including phenols, flavonoids, tannins, steroids, and saponins. Purpose: To prove and analyze the antibacterial effectiveness of rambai leaf extract (Sonneratia caseolaris) against the growth of Aggregatibacter actinomycetemcomitans bacteria. Method: True experimental with post test design only with control group and there are 7 treatment groups with 6 repetitions. Antibacterial test using liquid dilution method to determine the value of Minimum Inhibitory Content (MIC) and solid dilution to determine the value of Minimum Bactericidal Content (MBC). Data analysis using Kruskal Wallis and Post Hoc Mann Whitney test. Results: Based on the results and data analysis, the MIC value of rambai leaf extract was found at a concentration of 40%, while the MBC was found at a concentration of 100%. Conclusion: Rambai leaf extract is able to inhibit and kill Aggregatibacter actinomycetemcomitans bacteria. Keywords: Aggregatibacter actinomycetemcomitans, Aggressive Periodontitis, Sonneratia caseolaris ABSTRAKLatar belakang: Periodontitis agresif merupakan penyakit yang merusak jaringan periodontal dengan cepat yang disebabkan oleh dominasi bakteri Aggregatibacter actinomycetemcomitans sebesar 90%. Perawatan periodontitis agresif dapat berupa antibiotik. Salah satu antibiotik yang dapat dipakai yaitu metronidazole, namun metronidazole ini dapat memberikan efek samping apabila digunakan dalam jangka panjang seperti diantaranya sakit kepala, mual, mulut kering, dan berasa logam di mulut. Terdapat tumbuhan herbal yaitu daun rambai (Sonneratia caseolaris) yang mengandung senyawa yang dapat digunakan sebagai antibakteri diantaranya fenol, flavonoid, tanin, steroid, dan saponin. Tujuan: Membuktikan dan menganalisis efektivitas antibakteri ekstrak daun rambai (Sonneratia caseolaris) terhadap pertumbuhan bakteri Aggregatibacter actinomycetemcomitans. Metode: True experimental dengan desain posttest only with control group dan terdapat 7 kelompok perlakuan dengan 6 kali pengulangan. Uji antibakteri menggunakan metode dilusi cair untuk mengetahui nilai Kadar Hambat Minimum (KHM) dan dilusi padat untuk mengetahui nilai Kadar Bunuh Minimum (KBM). Analisis data menggunakan uji Kruskal Wallis dan Post Hoc Mann Whitney. Hasil: Berdasarkan hasil dan analisis data didapatkan nilai KHM ekstrak daun rambai pada konsentrasi 40%, sedangkan KBM terdapat pada konsentrasi 100%. Kesimpulan: Ekstrak daun rambai mampu menghambat dan membunuh bakteri Aggregatibacter actinomycetemcomitans. Kata Kunci: Aggregatibacter actinomycetemcomitans, Daun Rambai, Periodontitis Agresif
COMPARISON OF ANTIBACTERIAL CHITOSAN OF HARUAN FISH SCALES (Channa striata) WITH Chlorhexidine gluconate AGAINST Lactobacillus acidophilus Tomy Wira Wahyuda; Deby Kania Tri Putri; Muhammad Yanuar Ichrom Nahzi
Dentin Vol 7, No 2 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v7i2.9726

Abstract

Background: Lactobacillus acidophilus plays an important role in the development of caries, especially advanced caries. Mouthwash such as chlorhexidine is the main choice to prevent the colonization of caries-causing bacteria, but in long-term use it causes side effects, so that new, more biocompatible materials are needed, namely chitosan of Haruan fish scales (Channa striata), which contains an amine group (Nh2). Purpose: To analyze and compare the antibacterial effectiveness of chitosan haruan fish scales at concentrations of 2.5%, 5%, 7.5%, and 10% with chlorhexidine gluconate 0.2% on the growth of Lactobacillus acidophilus bacteria. Method: True Experimental Laboratory Research with a procedure consisting of making chitosan from Haruan fish scales through the processes of deproteination, demineralization, and deacetylization followed by an antibacterial test with 5 treatment groups on the growth of Lactobacillus acidophilus bacteria using the diffusion method with inhibition zone parameters on the test material. Result: Haruan fish scale chitosan has the lowest antibacterial effectiveness, namely a concentration of 2.5% with an average inhibition zone value of 8.6 mm, and the highest is a 10% concentration with an inhibition zone average value of 21.3 mm, and a concentration of Haruan fish scale chitosan. 10% had antibacterial effectiveness comparable to 0.2% chlorhexidine gluconate against the growth of Lactobacillus acidophilus bacteria. Conclusion: Haruan fish scale chitosan concentrations of 2.5%, 5%, 7.5%, and 10% had antibacterial effectiveness against the growth of Lactobacillus acidophilus bacteria, and a concentration of 10% was comparable to 0.2% chlorhexidine gluconate in inhibiting the growth of Lactobacillus acidophilus bacteria. Keywords : Caries, chitosan haruan fish scales, Lactobacillus acidophilus.
GAMBARAN PROFIL JARINGAN LUNAK SECARA KLINIS DAN FOTO SEFALOMETRI PADA SUKU BANJAR Aulia Rahimah; Diana Wibowo; Ika Kusuma Wardani; Aulia Azizah; Deby Kania Tri Putri
Dentin Vol 7, No 3 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v7i3.10744

Abstract

Latar Belakang: Salah satu penunjang akurat untuk penegakan diagnosa dalam perawatan ortodonti adalah analisis sefalometri. Analisis jaringan lunak pada wajah dapat dilakukan secara klinis dan sefalometri yang dikategorikan menjadi profil wajah cembung, cekung, dan lurus. Salah satu metode analisis sefalometri yang mampu menentukan profil wajah adalah Analisis rickets. Faktor ras dan keanekaragaman kultural sangat berpengaruh terhadap profil wajah seseorang. Tujuan: Menggambarkan profil jaringan lunak secara klinis dan foto sefalometri pada mahasiswa Suku Banjar FKG ULM. Metode: Penelitian deskriptif dengan pendekatan cross sectional. Teknik pengambilan sampel probability sampling menggunakan simple random sampling. Populasi adalah seluruh mahasiswa preklinik yang berasal dari Suku Banjar di Fakultas Kedokteran Gigi Universitas Lambung Mangkurat yang berjumlah 60 orang. Besar sampel minimal dihitung menggunakan rumus Deskriptif kategorik dan didapatkan hasil 39 sampel. Hasil: Analisis statistik deksriptif dari Analisis Rickett menunjukkan rerata jarak bibir atas terhadap garis estetik (Ls-E) adalah 0,34 mm dengan standar deviasi 0,25. Rerata jarak bibir bawah terhadap garis estetik (Li-E) adalah 1,66 mm dengan standar deviasi 2,63. Kesimpulan:  penelitian ini adalah secara klinik dan sefalometri menunjukkan mayoritas responden memiliki profil wajah cembung. Kata Kunci: Analisis Rickett, Profil jaringan lunak, Sefalometri, Suku Banjar.
BIODEGRADATION OF CHITOSAN MEMBRANE SCALES OF HARUAN FISH (Channa striata)-HYDROXYAPATITE IN ARTIFICIAL SALIVA SOLUTION Syifa Kamila; Deby Kania Tri Putri; Amy Nindia Carabelly; Melissa Budipramana; Bambang Setiawan
Dentin Vol 8, No 1 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v8i1.12192

Abstract

Background: Membrane materials for surgical procedures using Guided Tissue Regeneration (GTR) are Polytetrafluoroethylene (PTFE) and collagen, but have the disadvantage of requiring further surgical procedures to remove membranes after use and rapid degradation properties. Haruan (Channa striata) fish scale chitosan is expected to be an alternative material for GTR membranes. Purpose: To determine the longer degradation time between chitosan membranes and chitosan+HA membranes in artificial saliva. Methods: This study used true experimental with post test only with control design using three treatments of 5% chitosan, 5% + 2 gr chitosan, and 5% + HA 4 gr of chitosan, positive control of GTR membrane with time intervals of 1, 7, 14, and 21 days in artificial saliva. Results: The results of the Kruskall Wallis test showed that there was a significant difference, the Mann Whitney Post Hoc test yielded 5% chitosan with chitosan+2 gr HA and chitosan+2gr HA with chitosan+4 gr HA had a p value <0.05 p=(0.000) in the 14th day, 5% chitosan with chitosan+4gr HA had a p value <0.05 p=(0.000) significant on days 7 and 14, and the positive control with all treatments had a p value <0.05 p=(0.000) . Conclusion: Chitosan and chitosan+HA membranes resulted in a degradation time of 21 days. Chitosan membrane + 2 g HA membrane was the best treatment because the remaining weight of the membrane was greater than the other treatments.Keywords : Channa striata, Chitosan, Degradation Test, Guided Tissue Regeneration (GTR), Hydroxyapatite, Remaining Membrane Weight
PENGARUH PERENDAMAN GIGI PADA KITOSAN SISIK IKAN HARUAN (Channa striata) TERHADAP PELEPASAN FOSFAT Muhammad Hafly Fariz Asyraq; Deby Kania Tri Putri; Tri Nurrahman; Agung Satria Wardhana; Bambang Setiawan
Dentin Vol 8, No 1 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v8i1.12201

Abstract

Background: Chitosan solution from Haruan fish scales (Channa striata) can be used to maintain tooth enamel structure by reducing the rate of solubility of hydroxyapatite or tooth demineralization under acidic conditions. Chitosan haruan fish scale (Channa striata) has the potential to be an anti-demineralization agent. Objective: Analyze the effect of immersing teeth in chitosan solution of 2.5% and 5% Haruan fish scales (Channa striata) on levels of phosphate release. Method: This study used the true experimental method with a posttest-only control group design consisting of 3 treatment groups, namely the negative control, the 2.5% group and the 5% group. All groups were immersed in lactic acid solution pH 5.2. Measurement of phosphate release levels using a uv-vis spectrophotometer. Results: The results of the One Way Analysis of Variance (ANOVA) test obtained a value of p = 0.00 <0.05 which indicated that the hypothesis was accepted or that there was an effect of soaking the teeth in chitosan solution of Haruan fish scales (Channa striata) with a concentration of 2.5% and 5% on phosphate release . The Bonferroni Post Hoc Test showed that there were significant differences in all groups, namely the negative control group with the 2.5% and 5% groups, the 2.5% group and the 5% group. Conclusion: Chitosan from Haruan fish scales (Channa striata) concentrations of 2.5% and 5% are able to inhibit phosphate release on teeth and have the potential as alternative biomaterials in inhibiting tooth demineralization.Keywords: Caries, Chitosan, Demineralization, Haruan, Phosphate
PENGARUH PERENDAMAN GIGI DESIDUI PADA LARUTAN KITOSAN SISIK IKAN HARUAN (Channa striata) TERHADAP PELEPASAN ION KALSIUM Noval Ihza Maulana; Didit Aspriyanto; Deby Kania Tri Putri; Yusrinie Wasiaturrahmah; Sherli Diana
Dentin Vol 8, No 2 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v8i2.13109

Abstract

ABSTRACTBackground: Haruan fish (Channa striata) scale chitosan solution can maintain tooth structure by reducing the solubility rate of hydroxyapatite which makes up tooth enamel. Haruan fish (Channa striata) scale chitosan solution prevents tooth demineralization in an acidic environment. Objective: To analyze the effect of soaking deciduous teeth in chitosan solution from haruan fish scales (Channa striata) with concentrations of 1.25%, 2.5% and 5% on the release of calcium ions in an acidic environment. Method: Pure experimental research with a post-test only design with control group, consisting of 4 treatment groups. The negative control was immersion of deciduous teeth in lactic acid solution pH 5.2, and treatment groups with concentrations of 1.25%, 2.5% and 5%. All groups soaked deciduous teeth in lactic acid solution with a pH of 5.2 for each group. Measurement of calcium release levels in deciduous teeth using UV-Vis spectrophotometry instruments. Results: The results of this study are hypothesized to be acceptable or that there is an effect of soaking deciduous teeth in haruan fish scale chitosan (Channa striata) concentrations of 1.25%, 2.5% and 5% in inhibiting the release of calcium ions. This study showed that there were no significant differences between the 1.25%, 2.5% and 5% treatment groups, but there were significant differences between the control group and all treatment groups. Conclusion: Haruan fish (Channa striata) scale chitosan with concentrations of 1.25%, 2.5%, and 5% is able to inhibit the release of calcium in deciduous teeth and has potential as a demineralization inhibitor biomaterial.Keywords :  Caries, Calcium, Chitosan, Demineralization, Haruan.  ABSTRAK Latar Belakang: Larutan kitosan sisik ikan haruan (Channa striata) dapat menjaga struktur gigi melalui proses penurunan laju kelarutan hidroksiapatit penyusun enamel gigi. Larutan kitosan sisik ikan haruan (Channa striata) mencegah demineralisasi gigi dalam lingkungan asam. Tujuan: Menganalisis pengaruh perendaman gigi desidui pada larutan kitosan dari sisik ikan haruan (Channa striata) dengan konsentrasi 1,25%, 2,5%, dan 5% terhadap pelepasan ion kalsium dalam lingkungan asam. Metode: Penelitian eksperimental murni dengan desain post-test only with control group, terdiri dari 4 kelompok perlakuan. Kontrol negatif yaitu perendaman gigi desidui pada larutan asam laktat pH 5,2, dan kelompok perlakuan konsentrasi 1,25%, 2,5%, serta 5%. Semua kelompok perendaman gigi desidui dalam larutan asam laktat dengan pH 5,2 dilakukan pada setiap kelompok. Pengukuran kadar pelepasan kalsium gigi desidui menggunakan instrumen spektrofotometri UV-Vis. Hasil: Hasil penelitian ini hipotesis dapat diterima atau adanya pengaruh perendaman gigi desidui pada kitosan sisik ikan haruan (Channa striata) konsentrasi 1,25%, 2,5%, dan 5% dalam menghambat pelepasan ion kalsium. Pada penelitian ini menunjukkan tidak adanya perbedaan signifikan antar kelompok perlakuan 1,25%, 2,5%, dan 5%, namun terdapat perbedaan bermakna antara kelompok kontrol dengan semua kelompok perlakuan. Kesimpulan: Kitosan sisik ikan haruan (Channa striata) dengan konsentrasi 1,25%, 2,5%, dan 5% mampu menghambat pelepasan kalsium pada gigi desidui dan memiliki potensi sebagai biomaterial agen penghambat demineralisasi. Kata kunci : Demineralisasi, Haruan, Kalsium, Karies, Kitosan
Co-Authors Agung Satria Wardhana Aisyah Nur Zahra Aizar Agi Syahrial Aizar Agi Syahrial, Aizar Agi Alexander Sitepu Amalia Putri, Amalia Amy Nindia Carabelly Ariyati Retno Pratiwi, Ariyati Retno Aspriyanto, Didit Aulia Azizah Aulia Rahimah Aura Amelia Azilita Ananda Azwar Fida Maulana Baehaqi Bambang Setiawan Bayu Indra Sukmana Bayu Yordha Senggara Beta Widya Oktiani Candra Candra Dewi Nurdiana Dewi Retno Wahyu Widyaningrum Diana Wibowo Duhan Kanzu Balad Eny Febriyanti Erika Norfitriah Fatimah Maulideya Fatma Kirana Ferdy Juliannor Fajar Firdaus, I Wayan Arya Krishnawan Fitriyana, Annisa Hamdani, Riky Hatta, Isnur Hutami, Wijayanti Diah Wasi Ichrom Nahzi, Muhammad Yanuar Ika Kusuma Wardani Indah Listiana Kriswandini Jamilatun Nisa Juli Harnida Purwaningayu K. Khairiah Maharani Laillyza Apriasari Melati Ainun Nisa Melisa Budipramana, Melisa Melissa Budipramana Moch. Andhika Firdausi Rachfa Muhammad Alfi Ghifari Muhammad Hafly Fariz Asyraq Muhammad Irfan Fanshuri Muhammad Luthfi Muhammad Luthfi MUHAMMAD RAYHAN Muhammad Zainal Fikri Nadalia Malika Bilqis Nadiya Amalia Naila Khairiyah Ninda Andrea Haliza Noval Ihza Maulana Nurrahman, Tri Nurul Fitriyani Dewi Priyawan Rahmadi Rahma Dania A.J Rahmadella, Afifah Rahmadi, Priyawan Renie Kumala Dewi Robiyansyah, Hengki Rosihan Adhani, Rosihan Sarifah, Norlaila Senggara, Bayu Yordha Setyawardhana, Raden Harry Dharmawan Sherli Diana Siti Khairiah Siti Raudah Siti Rohmah Syifa Kamila Tomy Wira Wahyuda Utami, Juliyatin Putri Vena Paramita Djunaidy Wardhana, Agung Satria Wydiamala, Erida Yuniar Elsa Dwinuria Yusrinie Wasiaturrahmah