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All Journal Farmaka Jurnal Kesehatan Masyarakat Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Farmasi Klinik Indonesia Global Medical and Health Communication The Indonesian Biomedical Journal Majalah Farmasi dan Farmakologi (Trends in Pharmacy and Pharmaceutical Sciences) Pharmaceutical Journal of Indonesia Majalah Farmasetika Borneo Journal Of Pharmascientech Hubungan Tingkat Pengetahuan Petugas Pengelola Obat dengan Tingkat Ketersediaan Obat Di Puskesmas Kota Malang Medical Sains : Jurnal Ilmiah Kefarmasian Riset Informasi Kesehatan Medical Sains : Jurnal Ilmiah Kefarmasian Media Penelitian dan Pengembangan Kesehatan
Irma Melyani Puspitasari
Fakultas Farmasi Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Journal : The Indonesian Biomedical Journal

 1 
Modulation of Caspase-3 Expression by Arcangelisia flava Post Acetaminophen-Induced Hepatotoxicity in Rat’s Liver Steffi Liem; Tina Rostinawati; Ronny Lesmana; Sri Adi Sumiwi; Tiana Milanda; Mutakin Mutakin; Irma Melyani Puspitasari; Jutti Levita
The Indonesian Biomedical Journal Vol 10, No 2 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i2.412

Abstract

BACKGROUND: Acetaminophen, when used at low doses is a safe drug, but at higher doses it induces apoptosis in hepatoma cells. Arcangelisia flava that grows widely in Kalimantan Island, Indonesia, contains berberine which is effective in protecting the liver. This work was aimed to study the effect of A. flava extract on the modulation of caspase-3 in acetaminophen-induced hepatotoxicity.METHODS: Thirty-five Wistar male rats were divided into groups: I the normal control (water); II the negative control (Arabic gum powder or PGA, 2% in suspension); III the positive control (silymarin); IV-VII (A. flava extract 100, 200, 400, and 800 mg/Kg of body weight (BW), respectively) for 14 days. At day 15th, group II-VII were induced with acetaminophen 1000 mg/Kg of BW per oral for 7 days along with the extracts. At day 22nd, the animals were measured for their serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT) and gamma-glutamyl transferase (GGT), histological examination, and Western blotting.RESULTS: Acetaminophen elevated the SGOT and SGPT (3x compared to normal group), and GGT (5x compared to normal group) of the animals in group II. Pre-treatment with higher doses of A. flava extract (group VI and VII) significantly prevented the biochemical changes induced by acetaminophen. Normal histology of the liver was showed by group I, III, VII, whereas dilated sinusoids, central vein (CV) lesion, and local haemorrage were observed in group II, IV, V and VI. Western blotting showed an inhibition of caspase-3 expression by A. flava extract in dose-dependent manner.CONCLUSION: A. Higher dose A. flava extract shows hepatoprotective activity by preventing the elevation of serum transaminases and transferase levels. Eventually, no damage in the acetaminophen-induced rat’s liver was observed. This plant modulates the expression of caspase 3 protein in dose-dependent manner.KEYWORDS: Arcangelisia sp, caspase-3, berberine, hepatoprotective activity, NSAIDs, yellow root
Serum Free Zinc as A Predictor for Excessive Function of Pancreatic Beta-Cells in Central-Obese Men Miftakh Nur Rahman; Indriyanti Rafi Sukmawati; Irma Melyani Puspitasari; Miswar Fattah
The Indonesian Biomedical Journal Vol 11, No 3 (2019)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v11i3.618

Abstract

BACKGROUND: Central obesity is known as a risk factor for type 2 diabetes mellitus (T2DM). Its development is influenced by many factors such as a progressive failure of pancreatic beta cell function. The beta cells increase their function to secret insulin along T2DM development to compensate before it becomes exhausted. Zinc (Zn) plays a crucial role in beta cell function and insulin secretion. The majority of Zn in serum are bound to protein which is not readily available interact with cells. The free Zn in serum has been suggested as being more representative than total Zn in beta cell function. This research aimed to investigate the correlation between serum free Zn and homeostasis model assessment for beta cell function (HOMA-B) and to predict the pancreatic beta cell function in the development of T2DM.METHODS: This study was designed as an observational with a cross-sectional approach. The subjects were centrally obese men aged 30-50 years and who had met the inclusion and exclusion criteria from the screening tests. Control subjects were lean men without T2DM. Serum free Zn and serum total Zn were measured by using inductively coupled plasma-mass spectrometry (ICP-MS).RESULTS: There was positive correlation between serum free Zn and HOMA-B (R=0.361, p-value<0.001) but there was no correlation between serum total Zn and HOMA-B (R=-0.062, p-value=0.563). This study found that if the concentration of serum free Zn >1.7 ug/dL in central obese men was suggested as an excessive function of pancreatic beta cell and as an early warning before its exhausted.CONCLUSION: This study suggested that serum free Zn had a correlation with beta cell function and had a predictive ability for beta cell excessive function before its exhausted.KEYWORDS: Type 2 diabetes mellitus, HOMA-B, serum free zinc, central obesity 
Co-Authors Abdul Kholik Tasib AJENG RATNA NINGTYAS Alfathia, Kinanti Angela Aprilia Karyadi Anisah Dahlan Dewi Alfian, Sofa Dewi, Triwedya Indra DHEANDA, HILALLYA MAURIZKA Durotulailah, Lela Dwi Yuniati Daulay Eli Halimah, Eli Ellin Febrina Febio Gutama Fitria Hariati Ramdhani Gazali, Munawir Gofarana Wilar HILDA VILDAYANTI Ika Ratna Hidayati Indah Suasani Wahyuni Indriyanti Rafi Sukmawati Irma Erika Herawati Irma Erika Herawati Ivan Surya Pradipta Jutti Levita Khairunnisa, Shintani Ayunda Maratul Mahdiyyah Marulin, Dita Mas Rizky A. A. Syamsunarno Mas Rizky A.A. Syamsunarno Maulida, Riza Melisa Intan Barliana Miftakh Nur Rahman Miswar Fattah Mutakin Mutakin Mutakin Mutakin Neni Anggraeni Norisca Aliza Putriana NORISCA ALIZA PUTRIANA Oneng Ifayani Putra, Dimas Setyadi Rahayu, Cherry Rahmawati Witriani Witriani, Rahmawati Witriani RANO KURNIA SINURAYA Rendrayani, Farida Renny Amelia RESTU AMELIA APRIYANDI Rizka Novia Atmadani Ronny Lesmana Sari, Aisha Maulidya Satriasih, Tulus Satriasih Silanas, Ilman Sofa Dewi Alfian Sofa Dewi Alfian Sri Adi Sumiwi Sriwidodo Sriwidodo Steffi Liem Sumedhi, Herdina Mayangsari Thurfah, Jihan Nurul TIANA MILANDA TIARA DIMAS HAPSARI Tina Rostinawati UZLIFATUL ZANNAH Wakhid Rizka Saputra Widya N. Insani Yasmiwar Susilawati Yuliandani Yuliandani Yuliandani, Yuliandani Zakiyah, Neily