Article Per Year (5 Year)
p-Index From 2021 - 2026
2.766
P-Index
This Author published in this journals
All Journal Jurnal Natur Indonesia Jurnal Ilmu Kedokteran (Journal of Medical Science) Jurnal Kedokteran Brawijaya Global Medical and Health Communication The Indonesian Biomedical Journal Jurnal Ilmiah Pertanian Jurnal Pengabdian UntukMu NegeRI Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Gizi Klinik Indonesia Jurnal Kedokteran dan Kesehatan Manuju : Malahayati Nursing Journal PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Ners Acta Chimica Asiana Unri Conference Series: Community Engagement Jurnal Katalisator Biomedika Media Penelitian dan Pengembangan Kesehatan Biodiversitas Journal of Biological Diversity
Ismawati ismawati
Department Biochemistry, Faculty Of Medicine, Universitas Riau, Jl. Diponegoro No 1, Pekanbaru
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences

Published : 28 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : indonesian journal of medical laboratory science and technology

 1 
Vitamin D enhances migration but decreases gene expression of vascular endothelial growth factor and tumor necrosis factor-α in Wharton’s jelly mesenchymal stem cells Vitriyanna Mutiara Yuhendri; Nurfi Pratiwi; Ariza Julia Paulina; Ismawati Ismawati; Nicko Pisceski Kusika Saputra; Arfianti Arfianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in Biomarkers, Therapeutics, and Probiotics: Recent Updates in Medical L
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6698

Abstract

Mesenchymal stem cells (MSCs) have gained significant interest for their potential application in repairing damaged tissues through immunomodulatory functions. Vitamin D has been shown to regulate both the innate and adaptive immune systems. Active forms of Vitamin D, such as 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) are known to influence the action of mesenchymal stem cells (MSCs) in several mechanisms, such as stimulating their proliferation and osteogenic differentiation, as well as tissue regeneration. This study aimed to investigate the effects of vitamin D supplementation on the biological properties and expression of cytokines and growth factor genes in MSCs isolated from the human umbilical cord (UC). MSCs were isolated from Wharton’s jelly (WJ-MSC) of UC, cultured, and supplemented with various concentrations of vitamin D3. The Cell Counting Kit-8 (CCK-8) assay was used to measure cell viability, and a scratch wound healing assay was conducted to evaluate the migration capacity of MSCs. The mRNA expression levels of vascular endothelial growth factor (VEGF), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 were quantified using reverse transcription-polymerase chain reaction (RT-PCR). This study showed that supplementation with 50 nM of vitamin D3 for 48 h significantly increased the viability and migratory capacity of MSCs. Furthermore, vitamin D supplementation significantly decreased the mRNA levels of TNF-α and VEGF but did not affect IL-6 gene expression compared to the control group. These findings suggest that vitamin D supplementation can enhance the biological characteristics and modulate the expression of key immunomodulatory factors in MSCs, potentially improving the effectiveness of MSC-based therapies.
Co-Authors Adinda Aisyah Afif P Jati Afifah Ramadhani Angel Chen Anggraini, Suci Ardhi, Aulia Arfianti Arfianti Ariqa Rahma Abdullah Ariza Julia Paulina Cantika Natswa Deanra Cantika Natswa Deanra Deanra, Cantika Natswa Desby Juananda Dhea Ayu Kartini Surya Putri Dinda Salsabila Dyna, Fitri Edy Fachrial Effendi, Fallgivando Elfima Aditya Utami Enikarmila Asni Enikarmila Asni Enikarmila Asni Erwin Christianto Esy Maryanti Fitri Dyna Fitri Dyna Ilhami Romus Ilhami Romus Ilhami Romus Ilhami Romus Ilhami Romus, Ilhami Imelda EB Hutagaol Imelda EB Hutagaol Imelda Tresia Pardede Indah Putri Arwandi Irfani Dhia Rafif Irwan Irwan Isnani Kurniyanti Kesya, Lunari Ligat Pribadi Sembiring Lovenia, Nia Lunari Kesya Lusi Yusrenti Luthfianty, Elfiah M Derillovyandra Dwi Anugrah M Yulis Hamidy MERRY YOHANA SIPAYUNG Mifta Zahira Muhammad Ardaris Alhudri Muhammad Fadhillah Arif Muhammada Ardaris Alhudri Mukhyarjon NABELLA SURAYA Nabella Suraya Nabella Suraya Nazla Hafizah Nicko Pisceski Kusika Saputra NOVA WAHYU PRATIWI NOVA WAHYU PRATIWI, NOVA WAHYU Noviardi Noviardi, Noviardi Nurafriza, Widya Nurfi Pratiwi Permatasari, Nopi Putra, Amrullah Syah Qryoga Irsyad Reyen Raissa Maharani Putik Kusuma Rijalun Arridho Rudolf Fernando Turnip Salsabila Nurul Wildani Hasibuan Salsabila, Dinda Saputra, Nicko Pisceski Kusika Saryono Saryono Saryono Saryono Saryono Saryono Saryono Saryono SARYONO SARYONO Saryono Saryono Sasmita, Ellen Rosyelina Sera Faren SILVERA DEVI Silvera Devi Sonia Dinda Paramitha Sri Wahyu Maryuni Sri Yanti Sri Yanti Sri Yanti Suci Anggraini suraya, nabella Timotius Agung S Timotius Agung Soripada Titania T Nugroho Titania Tjandrawati Nugroho Veni Dayu Putri Veni Dayu Putri Veni Dayu Putri Vitriyanna Mutiara Yuhendri Winarto Winarto Zikrilla mayuli Hopi