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INDONESIA
Jurnal Biodjati
Published by Universitas Islam Negeri Sunan Gunung Djati Bandung
ISSN : 25481606     EISSN : 25414208     DOI : 10.15575/biodjati
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences
Arjuna Subject : -
Articles 15 Documents
 1   2 
Search results for , issue "Vol 7 No 1 (2022): May" : 15 Documents clear
Immunomodulatory Activity of Allium sativum, Curcuma mangga, and Acorus calamus Combination Nanoparticle on Mice Leukocytes Profile Muchtaromah, Bayyinatul; Analisa, Nur Izzah; Ahmad, Mujahidin; Fitriasari, Prilya Dewi; Habibi, Soraya; Hayati, Alfiah; Fajriyah, Ely Nuril
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.11879

Abstract

Immunomodulators are substances acting as stimulants or suppressants against the immune system, either to specific or non-specific activity. The non-specific through the production of leukocytes as the first response in fighting against an attacking antigen. Meanwhile, the specific, hrough recognition of antigens by lymphocytes during reattack. This research aimed to determine the immunomodulatory activity of the extracts combination nanoparticles of Allium sativum, Curcuma mangga, and Acorus calamus on the leukocyte profile of mice. This study used 5 treatments and 6 repetitions. The treatment groups were K- (untreated group), P1 (extracts combination nanoparticle dose of 25 mg/kg), P2 (extracts combination nanoparticle dose of 50 mg / kg), P3 (subur kandungan herbal medicine dose of 75 mg / kg), and P4 (Clomiphene citrate dose of 0.9 mg / kgBW). The parameters used included the total number of leukocytes and their differential value. The data that met the parametric assumptions, such as normally distributed and homogeneous were examined using the One Way ANOVA test, and when there was a significant difference, it was processed with the Duncan assessment. While those that did not meet the assumptions were evaluated using a non-parametric analysis. The statistical results showed that administration of extracts combination nanoparticles of A. sativum, C. mangga, and A. calamus at doses of 25 mg/kg and 50 mg/kg were able to suppress the inflammatory reaction by decreasing the total number of leukocytes. However, the differential leukocyte count was able to maintained or modulate immune system, indicating by the percentage of neutrophil, basophil, and eosinophil in the normal range. At a dose of 50 mg/kg, decreased the percentage of lymphocytes, while for monocytes, all dosage ranges were able to increase their number.
Phytochemistry Screening and Antioxidant Activities of Extract Pomegranate, Grape, Fig, and Olive in the Various Solvent Savitri, Evika Sandi; Holil, Kholifah; Resmisari, Ruri Siti
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.13424

Abstract

The active compounds of grape, pomegranate, olive, and fig have anthocyanins that potential as antioxidant are flavonoids. Flavonoids have potential as antioxidant  to prevent and therapy various oxidative stress and related diseases. This study aimed to examine the antioxidant activity of a combination of pomegranate, grape, fig, and olive extracts using the DPPH (diphenyl-2-picrylhydrazyl) method.  The maceration method used was maceration of dry Simplicia with methanol 95% solvent, fresh maceration with 95% methanol and dry Simplicia with 95% ethanol solvent. The results of the phytochemistry test showed several compounds found in the extract combination pomegranate, grapes, fig, and olives such as polyphenol, flavonoid, tannin, steroid/triterpenoid. The result of the antioxidant test showed the fresh maceration 95% methanol showed higher results with the IC50 of 25.22 with a potent antioxidant activity category.
Diversity and Abundance of Insects Pollinator of Chayote (Sechium edule (Jacq.) Swartz Alfawwaz, Muhammad Dzaky; Permana, Agus Dana; Putra, Ramadhani Eka
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.13881

Abstract

Chayote plants (Sechium edule) with monoecious characters require a pollination process. The pollination process requires pollinating agents to increase its productivity, one of which is insects. This research aimed to determine the diversity and abundance of insects pollinator on chayote plants. Observation of diversity and abundance used a scan sampling method. Pollinator insects observations were carried out in 3 time periods, morning, afternoon, and evening on male and female flowers. We measured environmental parameters such as temperature, humidity, wind speed, and light intensity. Eight species of wild insects pollinated chayote flowers, Apis cerana, Apis dorsata, Lasioglossum leucozonium, Polistes sagittarius, Phimenes flavopictus, Campsomeriella annulata, Lucilia sericata, and Musca domestica. The insect pollinators community had moderate diversity (1.23), a relatively dynamic community (0.59), and moderate dominance (0.62), with A. cerana, which had been the dominant insect pollinator with a relative abundance of 61.63%. Musca domestica and L. sericata were (0,58%) the least dominant insect pollinator with a relative abundance of 0.58%. This research concludes that the insects pollinator of chayote has a moderate level of diversity, relatively dynamic community, and average dominance.
Anti-inflammatory Activity of Bajakah Stem (Spatholobus littoralis Hassk.) Ethanolic Extract in Carrageenan-Induced Paw Edema Mice Rousdy, Diah Wulandari; Wardoyo, Elvi Rusmiyanto Pancaning; Ifadatin, Siti
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.14126

Abstract

Bajakah tampala plant (Spatholobus littoralis Hassk.) has been used for traditional medicine by the Kalimantan Dayak tribe in the treatment of cancer. This study aimed to find out the potential of ethanol extract of the stem of Bajakah tampala (Spatholobus littoralis Hassk.) as an anti-inflammatory agent in carrageenan-induced mice. The method used was a Completely Randomized Design with five treatments and five replications. The treatments consisted of a water-treated mouse as a negative control, sodium diclofenac 30,8 mg kg-1 as a positive control, and an extract of bajakah dose 2.5; 250; 1250 mg kg-1 BW. The dose of bajakah extract was given orally one hour before carrageenan induction. The inflammation induction used the Winter method by injecting 0.15 ml of 2% carrageenan suspension into the soles of mice. The diameter of edema was measured for 210 minutes. The results showed that the diameter of the mice soles showed a significant increase in all groups after the carrageenan injection. The value of AUC (Area Under the Curve) of bajakah extract dose (2.5; 250; 1250 mg kg-1) from the 150th minute to the 210th minute did not show significant differences from one another. The sodium diclofenac as positive control gave the lowest total AUC value of 236 mm.minute, followed by bajakah extract at a dose of 2.5 mg kg-1 BW of 239.22 mm.minute. The calculation results of the percentage of the inflammation inhibition showed that the administration of ethanol extract of the stem of Bajakah, Spatholobus littoralis, at the lowest dose of 2.5 mg kg-1 BW provide the best inflammation inhibition with 19.21% inhibitory value, nearly approaching the positive control of diclofenac sodium with an inhibitory value of 21.53%.
Anticancer Potency of Dimethyl 2-(2-Hydroxy-2-Methoxypropilidine) Malonate in Kombucha Taupiqurrohman, Opik; Rezaldi, Firman; Amalia, Dina; Suryani, Yani
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.14634

Abstract

Kombucha has an anticancer potency because it has dimethyl 2-(2-hydroxy-2-methoxypropilidine) malonate compound. The research aimed to verify the compound dimethyl 2-(2-hydroxy-2-methoxypropilidine) malonate as an anticancer with the in-silico method, namely the molecular docking approach, drug likeness profile, and ADMET test. The tools used were the PyRx, Discovery Studio Visualizer, Sanjeevini, and pkCSM. The research material consisted of 3D Dimethyl 2-(2-Hydroxy-2-Methoxypropilidine) Malonate and Epidermal Growth Factor Receptor (EGFR). The analysis showed Dimethyl 2-(2-Hydroxy-2-Methoxypropilidine) Malonate is safe for consumption and can suppress cancer cells. 
Analysis Effect of Nano Chitosan Coating on The Quality of Shallot Bulbs (Allium ascalonicum L. var. Bauji) Puspita, Della Widya; Prihastanti, Erma; Suedy, Sri Widodo Agung; Subagio, Agus
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.14900

Abstract

Shallots are bulb-shaped commodities that are difficult to maintain in new conditions because they are easily damaged. An effective effort to prevent deterioration of food quality during storage is by coating it using nano chitosan. The effectiveness of nano chitosan is influenced by particle size which related to the ratio of chitosan and STPP (sodium tripolyphosphate). This study aimed to determine the effect of nano chitosan coating with the addition of different ratios of chitosan and STPP and the appropriate ratio of chitosan and STPP on nano chitosan as a coating to maintain the quality of shallot bulbs (Allium ascalonicum L. var. Bauji). This study used a completely randomized design (CRD) method with four treatments and five replications. The treatments of this research were P0= Control, PI= Nano chitosan ratio of chitosan: STPP 1:3, P2= Nano chitosan ratio of chitosan:STPP 1:4, and P3= Nano chitosan ratio of chitosan:STPP 1:5. The variables of this study were the percentage of damage, the percentage of diameter shrinkage, weight loss, color, hardness, and moisture content. Data were analyzed using the ANOVA test and continued with the DMRT test. The application of nano chitosan coating on shallot bulbs could reduce damage, shrinkage of tuber diameter, weight loss, color, hardness, and decrease in water content better than the control treatment. The best results were shown by treatment P3 (1:5) with a percentage of damage of 8%, diameter shrinkage of 20.20%, weight loss of 18.40%, total color change of 54.45, hardness of 226.23 N, and a decrease in water content of 4.65% at the final water content of 79.09%.
Ethnoecology of The Slamet Mountain Slope Community (SMSC) in Paguyangan District, Brebes Regency, Central Java Fikriyya, Nabela; Helmanto, Hendra; Zukarnaen, Rizmoon Nurul; Nisyawati, Nisyawati; Silalahi, Marina
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.14909

Abstract

The local communities have local knowledge in utilizing and managing landscape units. The threat of modernization has led to the degradation of local knowledge, including the Javanese community on Mt. Slamet. This study aimed to analyze the plant diversity characteristics of landscape units and determine the important value of landscape units and plant species used by the community. This research was conducted in the Ragatunjung, Cipetung, and Pandansari village, Paguyangan District, Brebes Regency, Central Java. Semi-structured interviews collected Ethnoecological data with eight key informants and 83 respondents selected by purposive sampling and snowball sampling. Data on the level of landscape utilization were analyzed using the Local User's Value Index (LUVI). The community recognizes nine types of landscape units, such as perawisan (yard), wanah (production forest), Majegan (gardens), Sabin (rice fields), Kubang buyut (protected forest plan), tea plantations, Telaga Ranjeng Nature Reserve, Tanah Bengkok and tuk (water sources). Wanah was the most important landscape unit in the community in Ragatunjung (31.27), Cipetung (53.55), and Pandansari (28.17). Oryza sativa had the highest importance value in Sabin (22) and wanah (12) of Ragatunjung. In contrast, Syzygium aromaticum had the highest in Majegan (6.68). Brassica oleracea has the highest importance value in the two landscape units of Cipetung, namely, Majegan (4.20) and settlements (3.5), while in the wanah is Zea mays (11.38). Solanum tuberosum had the highest value in each landscape unit in Pandansari, both in wanah (10.33), Majegan (6.80), and Protection Forest Plan (PFP) (4.13). Categorizing landscapes and their utilization by maintaining certain landscapes, such as the Telaga Ranjeng Nature Reserve and Kubang buyut, directly impact the sustainability and integrity of ecosystems and natural resources in and around the area.
Protease and Amylase Activities of Javaen barb (Systomus rubripinnis Val.) Susilo, Untung; Rachmawati, Farida Nur; Wibowo, Eko Setio
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.15535

Abstract

Studies on morphology, growth, and reproduction have been carried out on wild Javaen barb, but there was no information on its digestive capacity; therefore, the research was conducted to determine protease and amylase activities in the digestive tract. This study used a total of 50 barbs with body weights between 13.56 -128.93g / fish. The measurement of enzyme activity was carried out using the spectrophotometer method. The results showed that differences in fish size resulted in differences in protease activity, but not for amylase. Fish with a small size have a higher protease activity than fish with a larger size. The protease activity did not differ between pH 6.9 to 10.0 but was higher than pH 12.5. Protease activity also did not vary between the anterior and posterior intestine and between 30-50°C. Amylase activity also found no difference between the anterior and posterior intestine, but there was a difference in activity between temperatures of 30-50°C. In conclusion, protease activity occurs in a neutral to alkaline environment, and there were differences in protease activity between different body sizes but not between intestinal segments. Amylase activity occurs throughout the intestine and decreases at temperatures of 50°C.
Comparative Analysis of Nutritional Content of Mudskipper Periophthalmus variabilis and Boleophthalmus boddarti Hidayat, Saifullah; Yusuf, Andi Muhammad; Kusuma, Hamdan Hadi
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.15604

Abstract

Gelodok or mudskipper fish are unique fish that have the ability to live in both aquatic and terrestrial areas. Some Indonesian who live in coastal areas use this fish for consumption. Types of mudskipper that are abundant in Indonesia are from the genera of Boleophtalmus, Periophthalmodon and Periophthalmus, where not all types have been studied for their nutritional content. The aim of this study was to analyze the content of Boleophthalmus boddarti and Periophthalmus variabilis. his study compared the nutritional content of the two types sampled from the Kaliwungu Kendal mangrove ecosystem and the Wedarijaksa Pati mangrove ecosystem. The two types of fish sampled were measured by morphometry which included body length, body width and body weight. Then the fish were analyzed for their nutritional content, namely carbohydrates using the Luff Schroorl test method, protein using the Kjeldahl method, fat using the Soxhlet method, iron using the AAS method, and phosphorus using the spectrophotometer method. The results showed that B. boddarti had a higher protein and iron content than P. variabilis . Meanwhile, P. variabilis had higher carbohydrate, fat and phosphorus content than B. boddarti. The difference was due to different feeding behavior, habitat, and types of food in B.boddarti and P. variabilis.
The Diversity of Reef Fish in Ulee Kareung waters, Bireuen District Indonesia Putra, Dedi Fazriansyah; Subqi, Muhammad Redha; Nasir, Muhammad; Purnawan, Syahrul; Setiawan, Ichsan; Fahal, Elzein Muhammed
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.16634

Abstract

Indonesia has abundant underwater natural resources, including coral reefs. Among coral reef ecosystems, reef fish make an important contribution to supporting the sustainability of underwater life. The more diverse reef fish in an ecosystem, it shows the healthier the condition of the coral reef ecosystem. The objective of the study was to investigate the diversity of the reef fish community including abundance, diversity, evenness, and dominance index in Ulee Kareung waters, Simpang Mamplam Sub-district, Bireuen District, Indonesia. We used a visual census technique (VCT)-belt transect by using a 50-meter transect and 3 times repetition at three observation stations. Each station had 2 depth categories such as shallow waters (3-5 m) and deep water (7-10 m). Results of the study found a total of 2094 individuals that consisted of 19 families and 59 species. The abundance value of reef fish ranged from 321 ind/ha - 610 ind/ha. The diversity index (H') ranged from 2.80 to 3.16. The evenness index (E) ranged from 0.79 to 0.88 and the dominance index (C) ranged from 0.06 to 0.10. Hence, it can be concluded that ulee kareung waters have a medium level of fish diversity.

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