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INDONESIA
Jurnal Biodjati
Published by Universitas Islam Negeri Sunan Gunung Djati Bandung
ISSN : 25481606     EISSN : 25414208     DOI : 10.15575/biodjati
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences
Arjuna Subject : -
Articles 482 Documents
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Nocturnal Coleoptera and Hemiptera Diversity at Giam Siak Kecil Bukit Batu Biosphere Reserve Indonesia Ruslan, Hasni; Tobing, Imran S. L.
Jurnal Biodjati Vol 6 No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.14102

Abstract

Giam Siak Kecil Bukit Batu is a biosphere reserve which one of its functions is as a habitat for wildlife. However, biodiversity data in the Giam Siak Kecil Bukit Batu Biosphere Reserve (GSKBB-BR) is still very minimal, including insects (Coleoptera and Hemiptera). This research was conducted to determine the diversity of Coleoptera and Hemiptera in the GSKBB Biosphere Reserve, Riau, Indonesia. The research was carried out using an exploratory method using "lights trap". The results of the study found 30 species, from 11 families of the order Coleoptera (23 species) and Hemiptera (7 species) in the GSKBB-BR. The diversity index of Coleoptera and Hemiptera at the observation site was moderate (H = 2.73), with a high evenness index (0.80). Scarabaeidae (order Coleoptera) is the family with the highest number of species found (8 species), while the most abundant species were Tibicen linnei and Pomponia fusca (Cicadidae/Hemiptera). Based on their functional roles, Coleoptera and Hemiptera with the highest number are herbivores (17 species), followed by predators (7 species) and decomposers (3 species). The range of values for temperature and humidity at the research site are in normal conditions. The GSKBB-BR area is an important remaining habitat for wildlife in Riau, including various types of insects (Coleoptera and Hemiptera); whose potential still needs to be revealed, and must be managed properly.
Habitat Preference Modeling of Prehistoric Giant Shark Megalodon During Miocene in Bentang Formation of West Java Coast Andriwibowo, Andriwibowo; Basukriadi, Adi; Nurdin, Erwin; Mubarok, Muh Aydava
Jurnal Biodjati Vol 6 No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.14115

Abstract

In the Miocene era about 20 million years ago, the South Coast of West Java was a sea and habitat for marine organisms including giant sharks Megalodon measuring about 18 meters long. This study aimed to model the habitat preference of the prehistoric gigantic shark Otodus megalodon population based on the fossil record. From fossil teeth, it revealed that the rock layer where the teeth found was Bentang formation from Miocene era. Many fossils of Megalodon had been unearthed from Bentang formation which is part of the South Coast of West Java. The habitat model was developed using the Sea Level Rise Inundation Tool of ArcGIS to estimate the sea depth and Megalodon’s habitat during the Miocene. The length of the teeth of O. megalodon found was ranged from 13 to 19 cm, indicating the presence of juvenile and adult O. megalodon. Based on the model, in the Miocene era, half of West Java was a sea with a depth ranging from 0 to 200 meters. At that time, it was estimated that juvenile O. megalodon occupied waters with a depth of 0-40 meters with an area of 1365 km2. Meanwhile, adult O. megalodon prefers a depth of 80-160 m and the frequency of habitat use increases at a depth of 200 m. The declining population of O. megalodon is associated with climate change and declining prey populations.
Anti-inflammatory Activity of Bajakah Stem (Spatholobus littoralis Hassk.) Ethanolic Extract in Carrageenan-Induced Paw Edema Mice Rousdy, Diah Wulandari; Wardoyo, Elvi Rusmiyanto Pancaning; Ifadatin, Siti
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.14126

Abstract

Bajakah tampala plant (Spatholobus littoralis Hassk.) has been used for traditional medicine by the Kalimantan Dayak tribe in the treatment of cancer. This study aimed to find out the potential of ethanol extract of the stem of Bajakah tampala (Spatholobus littoralis Hassk.) as an anti-inflammatory agent in carrageenan-induced mice. The method used was a Completely Randomized Design with five treatments and five replications. The treatments consisted of a water-treated mouse as a negative control, sodium diclofenac 30,8 mg kg-1 as a positive control, and an extract of bajakah dose 2.5; 250; 1250 mg kg-1 BW. The dose of bajakah extract was given orally one hour before carrageenan induction. The inflammation induction used the Winter method by injecting 0.15 ml of 2% carrageenan suspension into the soles of mice. The diameter of edema was measured for 210 minutes. The results showed that the diameter of the mice soles showed a significant increase in all groups after the carrageenan injection. The value of AUC (Area Under the Curve) of bajakah extract dose (2.5; 250; 1250 mg kg-1) from the 150th minute to the 210th minute did not show significant differences from one another. The sodium diclofenac as positive control gave the lowest total AUC value of 236 mm.minute, followed by bajakah extract at a dose of 2.5 mg kg-1 BW of 239.22 mm.minute. The calculation results of the percentage of the inflammation inhibition showed that the administration of ethanol extract of the stem of Bajakah, Spatholobus littoralis, at the lowest dose of 2.5 mg kg-1 BW provide the best inflammation inhibition with 19.21% inhibitory value, nearly approaching the positive control of diclofenac sodium with an inhibitory value of 21.53%.
Effects of Passiflora foetida on the Number of Purkinje Cells in the Cerebellum of Albino Rats Susilowati, Rina Priastini; Hartono, Budiman
Jurnal Biodjati Vol 7 No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.14298

Abstract

 Permot (Passiflora foetida) is one type of plant that has been used as a mosquito coil. Besides being effective at killing Aedes aegypti mosquitoes, mosquito coils made from permot leaf extract are also effective at killing German cockroaches and house flies. Until now there have been no reports stating the side effects of using permot leaf extract in humans. This study was designed to demonstrate the harmful side effects of inhalation of smoked mosquito coils made from permot leaf extract on the cerebellar cortex of adult male rats. Thirty-five adult male rats were used in this study. Rats were divided into 7 groups, namely group 1 as a negative control (without exposure), group 2 as a positive control (transfluthrin exposure 3000 ppm), groups 3 to 7 were treatment groups of permot leaf extract with graded doses (500 ppm, 1000 ppm, 2000 ppm, 3000 ppm, and 4000 ppm), and was treated for 12 weeks. All rats were sacrificed in a timely manner and cerebellar specimens were taken, prepared and observed using a light microscope. Previously, rats were weighed periodically to determine the effect of toxicity. The results showed that exposure to mosquito coil smoke made from 3000 ppm Transflutrin and 4000 ppm permot leaf extract caused weight loss in rats. Likewise on the weight of the rat cerebellum. The effect of decreasing the number of Purkinje cells also occurred in the group exposed to mosquito coils made from Transflutrin 3000 ppm and permot leaf extract 4000 ppm. Therefore, the safe and effective dose of mosquito coils made from permot leaves is less than 4000 ppm, because exposure to doses of more than 4000 ppm can cause degenerative changes in the cerebellar cortex.
Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers Solihah, Jumailatus; Kurniatanty, Isma; Subositi, Dyah; Maruzy, Anshary; Martiwi, Ika Nugraheny Ari; Ainy, Erny Qurrotul; Anam, Khoirul; Dina, Aslikh Lana
Jurnal Biodjati Vol 6 No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.14553

Abstract

Sida rhombifolia is one of wild flowering plants that grows easily in many habitats with moderate humidity, with some usefulness in traditional medicine. Genetic characterization of Sida rhombifolia accessions originated from 12 ethnicities of Indonesia was analyzed based on Sequence-Related Amplified Polymorphism (SRAP) Markers. The genomic DNA were extracted from leaf samples and then were characterized by using the SRAP marker system according to Li and Quiros (2001). Nine pairs of SRAP primer resulted high polymorphic bands and were used in the genetic profiling. The data analysis was performed using GenAlEx to calculate genetic distance, Principal coordinate analysis, and Analysis of Molecular Variance (AMOVA), also using POPGENE to assess genetic diversity (Hs and Ht) and Nm to predict gene flow among populations. The coordinate analysis showed that the accessions originated from ethnicities along Wallacean line tend to differ genetically from most other locations. However, the results of analysis of molecular variance suggested that there were only slight differences (0.1%) found between ethnicities, while most genetic variances (99.9%) were found mostly among accessions within populations. The results suggested that there was an extensive genetic flow and plant spreading among Sida rhombifolia plant populations, resulting more homogenous genetic characters among most populations, while high diversity within population. The calculation of the number of migration (Nm = 1.7341) confirmed that the high rate of gene flow had occurred between populations.
Bioconversion of Fermented Barley Waste by Black Soldier Fly Hermetia illucens L. (Diptera; Stratiomyidae) Permana, Agus Dana; Rohmatillah1, Din Dzakamala Fafi; Putra, Ramadhani Eka; Julita, Ucu; Susanto, Agus
Jurnal Biodjati Vol 6 No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.14609

Abstract

Black Soldier Fly Larvae (BSFL), Hermetia illucens (Diptera: Stratiomyidae) are widely used as bioconverter agents for various organics waste, and BSF pupae are often used as fodder for poultry and fish, because the BSF pupae have a high protein content. This study focused on applying BSFL as a bioconversion agent of the fermented barley waste to convert it to larvae biomass. Prior to application, barley waste was fermented either using effective microorganisms-4 (EM4), leachate, and water for seven days. The fermented barley waste was applied as feeding material for BSFL at the rate of 100 mg/larvae/days. As control commercial chicken fed (CF) was applied as feeding material at a similar feeding rate. During this study, waste reduction index (WRI), and efficiency of digested feed (ECD) were calculated, and the protein content in the BSF prepupae was analyzed. The results of this study showed that BSFL fed with CF produces the shortest development time (27 days), and high consumption rate. BSFL fed with barley waste fermented with EM4 (BE) and Leachate (BL) produces a larval period of 31 and 30 days respectively, and statistically those were not significantly different from control. This study showed that treatments of BE and BL, produced a very high larval survival rates, 98.67% and 97.00% respectively, and those two treatments were not statistically different from the control (96.67%). Although the control treatment resulted in a higher WRI compared to the other treatments, but the ECD of BE and BL treatments were higher than the ECD of the control. From this study, it can be concluded that BSFL has a good ability to convert fermented barley waste as well as controls, and the prepupae has a high protein content (42%), so BSFL fed with fermented barley waste has the opportunity to be used as a fed for poultry and fish.
Anticancer Potency of Dimethyl 2-(2-Hydroxy-2-Methoxypropilidine) Malonate in Kombucha Taupiqurrohman, Opik; Rezaldi, Firman; Amalia, Dina; Suryani, Yani
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.14634

Abstract

Kombucha has an anticancer potency because it has dimethyl 2-(2-hydroxy-2-methoxypropilidine) malonate compound. The research aimed to verify the compound dimethyl 2-(2-hydroxy-2-methoxypropilidine) malonate as an anticancer with the in-silico method, namely the molecular docking approach, drug likeness profile, and ADMET test. The tools used were the PyRx, Discovery Studio Visualizer, Sanjeevini, and pkCSM. The research material consisted of 3D Dimethyl 2-(2-Hydroxy-2-Methoxypropilidine) Malonate and Epidermal Growth Factor Receptor (EGFR). The analysis showed Dimethyl 2-(2-Hydroxy-2-Methoxypropilidine) Malonate is safe for consumption and can suppress cancer cells. 
Analysis Effect of Nano Chitosan Coating on The Quality of Shallot Bulbs (Allium ascalonicum L. var. Bauji) Puspita, Della Widya; Prihastanti, Erma; Suedy, Sri Widodo Agung; Subagio, Agus
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.14900

Abstract

Shallots are bulb-shaped commodities that are difficult to maintain in new conditions because they are easily damaged. An effective effort to prevent deterioration of food quality during storage is by coating it using nano chitosan. The effectiveness of nano chitosan is influenced by particle size which related to the ratio of chitosan and STPP (sodium tripolyphosphate). This study aimed to determine the effect of nano chitosan coating with the addition of different ratios of chitosan and STPP and the appropriate ratio of chitosan and STPP on nano chitosan as a coating to maintain the quality of shallot bulbs (Allium ascalonicum L. var. Bauji). This study used a completely randomized design (CRD) method with four treatments and five replications. The treatments of this research were P0= Control, PI= Nano chitosan ratio of chitosan: STPP 1:3, P2= Nano chitosan ratio of chitosan:STPP 1:4, and P3= Nano chitosan ratio of chitosan:STPP 1:5. The variables of this study were the percentage of damage, the percentage of diameter shrinkage, weight loss, color, hardness, and moisture content. Data were analyzed using the ANOVA test and continued with the DMRT test. The application of nano chitosan coating on shallot bulbs could reduce damage, shrinkage of tuber diameter, weight loss, color, hardness, and decrease in water content better than the control treatment. The best results were shown by treatment P3 (1:5) with a percentage of damage of 8%, diameter shrinkage of 20.20%, weight loss of 18.40%, total color change of 54.45, hardness of 226.23 N, and a decrease in water content of 4.65% at the final water content of 79.09%.
Ethnoecology of The Slamet Mountain Slope Community (SMSC) in Paguyangan District, Brebes Regency, Central Java Fikriyya, Nabela; Helmanto, Hendra; Zukarnaen, Rizmoon Nurul; Nisyawati, Nisyawati; Silalahi, Marina
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.14909

Abstract

The local communities have local knowledge in utilizing and managing landscape units. The threat of modernization has led to the degradation of local knowledge, including the Javanese community on Mt. Slamet. This study aimed to analyze the plant diversity characteristics of landscape units and determine the important value of landscape units and plant species used by the community. This research was conducted in the Ragatunjung, Cipetung, and Pandansari village, Paguyangan District, Brebes Regency, Central Java. Semi-structured interviews collected Ethnoecological data with eight key informants and 83 respondents selected by purposive sampling and snowball sampling. Data on the level of landscape utilization were analyzed using the Local User's Value Index (LUVI). The community recognizes nine types of landscape units, such as perawisan (yard), wanah (production forest), Majegan (gardens), Sabin (rice fields), Kubang buyut (protected forest plan), tea plantations, Telaga Ranjeng Nature Reserve, Tanah Bengkok and tuk (water sources). Wanah was the most important landscape unit in the community in Ragatunjung (31.27), Cipetung (53.55), and Pandansari (28.17). Oryza sativa had the highest importance value in Sabin (22) and wanah (12) of Ragatunjung. In contrast, Syzygium aromaticum had the highest in Majegan (6.68). Brassica oleracea has the highest importance value in the two landscape units of Cipetung, namely, Majegan (4.20) and settlements (3.5), while in the wanah is Zea mays (11.38). Solanum tuberosum had the highest value in each landscape unit in Pandansari, both in wanah (10.33), Majegan (6.80), and Protection Forest Plan (PFP) (4.13). Categorizing landscapes and their utilization by maintaining certain landscapes, such as the Telaga Ranjeng Nature Reserve and Kubang buyut, directly impact the sustainability and integrity of ecosystems and natural resources in and around the area.
Protease and Amylase Activities of Javaen barb (Systomus rubripinnis Val.) Susilo, Untung; Rachmawati, Farida Nur; Wibowo, Eko Setio
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.15535

Abstract

Studies on morphology, growth, and reproduction have been carried out on wild Javaen barb, but there was no information on its digestive capacity; therefore, the research was conducted to determine protease and amylase activities in the digestive tract. This study used a total of 50 barbs with body weights between 13.56 -128.93g / fish. The measurement of enzyme activity was carried out using the spectrophotometer method. The results showed that differences in fish size resulted in differences in protease activity, but not for amylase. Fish with a small size have a higher protease activity than fish with a larger size. The protease activity did not differ between pH 6.9 to 10.0 but was higher than pH 12.5. Protease activity also did not vary between the anterior and posterior intestine and between 30-50°C. Amylase activity also found no difference between the anterior and posterior intestine, but there was a difference in activity between temperatures of 30-50°C. In conclusion, protease activity occurs in a neutral to alkaline environment, and there were differences in protease activity between different body sizes but not between intestinal segments. Amylase activity occurs throughout the intestine and decreases at temperatures of 50°C.

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