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The Potential of Bacillus sp.’s Isolate of Coffee Beans as Plant Pest Control Candidates Ekowati, Christina Nugroho; Siallagan, Maria Denada; Sumardi, Sumardi; Rosa, Emantis
Akta Agrosia Vol 25 No 2 (2022)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Long-term use of chemical pesticides in high doses might result in chemical residues. Therefore, eco-friendly plant pest control should be getting more attention. To do so, one might consider the utilization of microorganisms that have the enzymatic ability to destroy the pest's body structure. This study aimed to explore the isolates of Bacillus sp. from coffee beans as a plant pest control candidate. Bacillus could be considered due to its ability to produce protein crystals and extracellular enzymes. Three isolates are obtained from isolation from coffee beans: Bacillus sp. 1; Bacillus sp. 2; and Bacillus sp. 3. These isolates have the enzymatic character of protease, chitinase, and lipase. The results of the bioassay test on larvae of Plutella xylostella (Lepidoptera order) showed that these isolates were able to cause larvae death within 48 hours.  
Pembuatan Kompos Daun Kering Dari Sampah Lingkungan Dusun Pal 6 Desa Karang Sari, Kecamatan Jati Agung Rosa, Emantis; Yulianti; Marcelia, Selvi; Septiani, Linda
AMMA : Jurnal Pengabdian Masyarakat Vol. 4 No. 3 : April (2025): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

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Abstract

Compost is a material produced from the decomposition process of organic matter, such as wet or dry, husk, garbage and so on. The benefits of compost, apart from reducing organic waste by turning it into useful materials, can improve soil quality and increase plant productivity.. The purpose of this community service activity is to provide knowledge, understanding and skills in making compost fertilizer from dry leaves found in the environment to residents in Dusun Pal 6, Desa Karang Sari, Kecamatan Jati Agung. The results of the assessment show that there is an increase in knowledge and understanding of the materials provided with an initial percentage of 52.0% increased by 77.05% with an average increase of 28.3%, the highest increase range is 41% and the lowest is 16%
Effectiveness of Ovitrap Against Aedes aegypti Mosquito In Kemiling Raya Sub-District Bandar Lampung City And The Vulnerability Of Its Larvals To Temephos Indriyani, Iin; Rosa, Emantis; Dania Pratami, Gina; Nukmal, Nismah
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 1 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i1.205

Abstract

Demam Berdarah Dengue (DBD) adalah salah satu masalah kesehatan masyarakat, yang disebabkan oleh Virus Dengue. Di Indonesia kasus DBD masih tinggi, khususnya di Lampung, salah satu Kota dengan angka kasus DBD yang masih tinggi adalah Bandar Lampung. Dari berbagai hasil penelitian diketahui ovitrap memiliki fungsi monitoring serta pengendalian Aedes sp.  Upaya pengendalian vektor nyamuk Ae. aegypti banyak dilakukan baik secara alami ataupun dengan cara kimia, cara kimia yang paling umum digunakan adalah penggunaan temephos. Penggunaan insektisida kimia seperti temephos secara terus menerus dapat menyebabkan resistensi. Tujuan penelitian untuk mengetahui efektivitas ovitrap di dalam dan luar rumah, mengetahui ovitrap index, serta status kerentanan larva nyamuk Ae. aegypti terhadap temephos di Kelurahan Kemiling Raya Kota Bandar Lampung. Penelitian dilakukan bulan Februari-April 2021. Ovitrap diletakkan di 142 rumah di dalam dan luar rumah, hasil telur dihitung, dipelihara dan diuji kerentananya. Bahan yang digunakan larva instar III hasil pemeliharaan hingga F1. Penelitian dilakukan dengan 4 kali ulangan, menggunakan 25 larva pada tiap gelas dengan larutan temephos 0,02 mg/L dalam 250 mL air dibiarkan kontak uji selama 1 jam dan masa pemulihan selama 24 jam. Hasil yang di dapat 83,10% rumah terdapat telur pada ovitrap dan 16,90% tidak ada telur, jumlah telur pada ovitrap di luar rumah (57%) lebih banyak dari dalam rumah (43%) serta ovitrap index yang didapatkan adalah 72,5%, persentase kematian 99% dan dikategorikan rentan.     Dengue Hemorrhagic Fever is one of the public health problems, which is caused by the Dengue Virus. In Indonesia, dengue cases are still high, especially in Lampung, one of the cities with a high number of dengue cases is Bandar Lampung. From various research results, it is known thatovitrap has the function of monitoring and controlling Aedes sp. Efforts to control the mosquito vector of Ae. aegypti is mostly done either naturally or chemically, the most commonly used chemical method is the use of temephos. The continuous use of chemical insecticides such as temephos can cause resistance. The purpose of the study was to determine the effectiveness of ovitrapinside and outside the home, to determine the ovitrap index, and the susceptibility status of Ae. aegypti against temephos in Kemiling Raya Village, Bandar Lampung City. The study was conducted in February-April 2021. Ovitrapswere placed in 142 houses inside and outside the house, egg yields were counted, maintained, and tested for susceptibility. Materials used for larvae instar III reared up to F1. The study was carried out with 4 replications, using 25 larvae in each glass with a solution of temephos 0.02 mg/L in 250 mL of water and allowed to contact the test for 1 hour and the recovery period for 24 hours. The results obtained were 83.10% of the house contained eggs in the ovitrap and 16.90% had no eggs, the number of eggs in the ovitrap outside the home (57%) was more than inside the house (43%) and the ovitrap index obtained was 72, 5%, the percentage of deaths is 99% and is categorized as vulnerable.    
Ovicidal Potential of Ethanol Extract of Cherry Leaves (Muntingia calabura L.) against Aedes aegypti Mosquito: Potensi ovisida Ekstrak Etanol Daun Kersen (Muntingia calabura L.) terhadap Nyamuk Aedes aegypti Lubis, Lulu Elsarah; Rosa, Emantis; Pratami, Gina Dania; Ernawiati, Eti
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 1 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v12i1.408

Abstract

Dengue fever is one of the viral diseases transmitted through arthropods and is still a significant health problem worldwide. Dengue virus is transmitted by Aedes aegypti mosquitoes from one human to another through blood sucking. These mosquitoes usually live and lay eggs in habitats close to stagnant water. The usual efforts to control Ae. aegypti are using natural and synthetic chemicals. Compounds to control Ae. aegypti eggs are called ovicides. One of the natural ingredients that is thought to contain natural chemicals that have the potential to be used as an ovicide is cherry leaves (Muntingia calabura L.). This study aims to determine the potential of the ethanol extract of cherry leaves (M. calabura L.) as an ovicide against Ae. aegypti. Five treatments and four replications were used. The results of this study indicate that the ethanol extract of cherry leaves (M. calabura L.) can potentially be an insecticide for Ae. aegypti at a concentration of 1% and an exposure time of 72 hours.
Mortalitas Kutu Kebul (Bemisia tabaci (Gennadius, 1889)) pada Tanaman Cabai (Capsicum annuum L.) yang Terpengaruh oleh Ekstrak Metanol Daun Singkong (Manihot esculenta Crantz.) Pury, O Ruming; Rosa, Emantis; Mumtazah, Dzul Fithria; Yulianty, Yulianty
Berkala Ilmiah Biologi Vol 16 No 1 (2025)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v16i1.20739

Abstract

Chili is a highly popular commodity plant among the public. However, domestic chili productivity often declines due to pest infestations. Whiteflies are one of the pests that can affect chili production yields. Cassava leaves serve as an alternative for producing natural insecticides to combat whitefly infestations. The objective of this study is to determine the phytochemical compounds present, evaluate the effectiveness of cassava leaf methanol extract, and identify the optimal concentration of cassava leaf methanol extract and observation time for the highest whitefly mortality rate on chili plants. This research is a factorial experimental study with two factors using a Completely Randomized Design (CRD). The first factor is the concentration of cassava leaf methanol extract (10%, 20%, 30%, and 40%), along with a negative control (distilled water) and a positive control (40% methomyl). The second factor is the observation time, which consists of 0.5 hours, 1 hour, 2 hours, and 24 hours after treatment. The obtained data were analyzed using SPSS, followed by a Tukey test. Phytochemical tests revealed that the methanol extract of cassava leaves contains flavonoids, saponins, tannins, and cyanide acid (HCN). Probit analysis results showed an LC50 value at a concentration of 32.61%. The Tukey test results indicated that cassava leaf methanol extract had the most significant effect on whitefly mortality at a 40% concentration with a treatment duration of 2 hours.
Hunungan Pengetahuan dan Tingkar Pendidikan dengan Perilaku Pencegahan Demam Berdarah di Desa Muara Gading Mas Ningrum, Adenias Lutfia; Rosa, Emantis; Carolia, Novita; Karyus, Aila
Jurnal Dunia Kesmas Vol 13, No 2 (2024): Volume 13 Nomor 2
Publisher : Persatuan Dosen Kesehatan Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jdk.v13i2.15854

Abstract

Demam berdarah dengue merupan penyakit infeksi disebabkan oleh virus dengue dengan vektor perantara nyamuk Aedes aegypti. Saat ini jumlah kasus DBD mencapai 71.633, salah satu kasus tertinggi yaitu terjadi di provinsi lampung dengan IR 64,4/100.000 penduduk, CFR 0,3 %. Seiring meningkatnya DBD pemerintah memberikan kebijaka terhadap pencegahan DBD dengan cara melakukan PSN 3M Plus. Tujuan dari penelitian ini yaitu untuk mengetahui hubungan pengetahuan dan tingkat pendidikan masyarakat dengan perilaku pencegahan penyakit DBD di desa muara gading mas kecamatan labuhan maringgai kabupaten lampung timur. Metode yang digunakan observasional pendekatan cross sectional, dibutuhkan 94 sampel, pemilihan sampe berdasarkan kriteria insklusi dan eksklusi lalu menggunakan teknik sample random sampling, data diambil menggunakan kuesioner pengetahuan dan perilaku pencegahan, kemudian dioleh dan dianalisis menggunakan analisis univariat dan bivariat yaitu uji chii-square. Hasil perilaku pencegahan dengan hubungan pengetahuan didapatkan p-value 0,001 dan dengan hubungan tingkat pendidikan masyarakat didapatkan p-value 0,001. Hasil tersebut menyatakan bahwa terdapat hubungan yang signifikan antara pengetahuan dan tingkat pendidikan masyarakat dengan perilaku pencegahan DBD di desa muara gading mas kecamatan labuhan maringgai kabupaten lampung timur. Kata kunci: pengetahuan, tingkat pendidikan, perilaku pencegahan DBD.
Isolasi dan Identifikasi Jamur Entomopatogen Sebagai Kandidat Bioinsektisida Lalat Rumah (Muscadomestica) Septiana, Nofita; Rosa, Emantis; Ekowati, Christina Nugroho
Biosfer: Jurnal Tadris Biologi Vol 10 No 1 (2019): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v10i1.4083

Abstract

Lalat rumah (M. domestica) merupakan vektor mekanik berbagai penyakit oleh mikroba patogen antara lain Salmonella penyebab demam tifoid, Shigella penyebab disentri, dan E. coli penyebab diare. Pengendalian M. domestica umumnya menggunakan insektisida sintetis, namun menimbulkan resistensi dan berdampak buruk bagi lingkungan. Oleh karena itu, perlu dilakukan pengendalian alternatif berupa pengendalian biologi menggunakan isolat jamur entomopatogen sebagai bioinsektisida. Penelitian ini diawali dengan isolasi jamur entomopatogen menggunakan metode moist chamber dengan larva M. domestica sebagai serangga pancingan. Jamur yang tumbuh pada larva dikultur dan dimurnikan pada media PDA lalu diidentifikasi. Identifikasi dilakukan melalui pengamatan makroskopis meliputi warna dan diameter koloni dan pengamatan mikroskopis meliputi struktur konidia, konidiofor, hifa, vesikula, fialid, dan sel kaki. Hasil isolasi dan identifikasi diperoleh lima jenis isolat yaitu Aspergillus sp. 1, Aspergillus sp. 2, Geotrichum sp., Penicillium sp., dan Aspergillus sp. 3.
Identifikasi Jenis Serangga Pada Tanaman Jeruk BW (Citrus reticulata Blanco) Menggunakan Metode Yellow Plastik Trap Dan Pitfall Trap Wiliam Binanta, Made; Rosa, Emantis; Dania Pratami, Gina
BULLET : Jurnal Multidisiplin Ilmu Vol. 4 No. 4 (2025): BULLET : Jurnal Multidisiplin Ilmu (INPRESS)
Publisher : CV. Multi Kreasi Media

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Abstract

Various obstacles can occur in citrus cultivation, including insect attacks as pests, predators, decomposers, pollinators, and parasitoids. This research was carried out from November to December 2024 at the Lampung Agricultural Training Center, using two traps, yellow plastic traps and trap traps, which aimed to determine the type, composition, and structure of insects. The results of insect identification and composition with yellow plastic traps found 10 species, 5 orders and 13 insect families. The results of identification and composition of trap traps found 5 species, 4 orders and 5 insect families. Analysis results for insect structure: The relative abundance in both yellow plastic traps and trap traps had a value of one (even abundance); Diversity index at 2.560 was categorized as moderate; Dominance Index 0.094 (categorized as low). In trap traps, the diversity index was 1.598 (moderate); Dominance Index 0.243 (low).
Jumlah dan Kemelimpahan Telur Aedes sp. di Ovitrap dan Kerentanan Aedes aegypti Terhadap Abate Widyastuti, Elyza; Rosa, Emantis; Pratami, Gina Dania; Kanedi, M
Bioma : Jurnal Biologi dan Pembelajaran Biologi Vol. 8 No. 1 (2023): BIOMA: JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v8i1.374

Abstract

Demam Berdarah Dengue ialah masalah kesehatan yang disebabkan oleh infeksi virus Dengue melalui perantara nyamuk Aedes aegypti. Berbagai upaya pengendalian sudah dilakukan namun belum memberikan hasil yang memuaskan. Salah satu upaya yang dapat dilakukan ialah dengan menggunakan ovitrap untuk mengetahui jumlah dan kemelimpahan telur. Penelitian ini dilaksanakan pada bulan Februari hingga April 2021, di tiga Lingkungan (LK) Kelurahan Way Halim. Tujuan penelitian ini untuk mengetahui jumlah dan kemelimpahan telur nyamuk Aedes sp. pada ovitrap dan kerentanan larva nyamuk Ae.aegypti terhadap abate. Hasil penelitian menunjukkkan bahwa jumlah telur yang terperangkap pada ovitrap baik di dalam mapun di luar rumah tertinggi adalah di LK II sebesar 558 butir Aedes sp, sedangkan Indeks kemelimpahan telur Aedes.sp di luar rumah lebih tinggi dari di dalam rumah yaitu sebanyak 1024 butir. Hasil uji kerentanan larva Ae.aegypti terhadap Abate setelah 24 jam terpapar menunjukkan bahwa 99% larva Ae. aegypti mengalami kematian, keadaaan ini dikategorikan dengan status rentan.
Pengenalan Fitotelmata Sebagai Tempat Perindukan Nyamuk Penular Penyakit Pada Masyarakat Desa Banjar Kanedi, Mohammad -; Sutyarso, Sutyarso; Rosa, Emantis; Abdullah Setiawan, Wawan
Jurdimas (Jurnal Pengabdian Kepada Masyarakat) Royal Vol. 7 No. 4 (2024): Oktober 2024
Publisher : STMIK Royal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33330/jurdimas.v7i4.3386

Abstract

In order to increase community participation in preventing the spread of mosquito-borne diseases, education for the community needs to be carried out through counseling. Tanggamus Regency is one of the areas in Lampung where DHF occurred, where from January to June 2024 there were 227 cases. To increase public knowledge in Banjar Agung Udik Village, Pugung District, Tanggamus Regency regarding phytotelmata as a breeding ground for mosquitoes, this counseling was carried out. Counseling was carried out using the pretest, lecture and discussion methods, and practicum. The results of the counseling showed that most participants knew that DBD and malaria were transmitted by mosquitoes, but not all knew the name of the vector species. Participants knew where mosquitoes perched at home and how to avoid mosquito bites. Participants also know mosquito breeding places, but are new to phytotelmata in this counseling. The practicum carried out by participants successfully identified six plant species that were proven to be phytotelmata. Thus, it can be concluded that this counseling has succeeded in providing knowledge to the community about the potential of phytotelmata as a source of mosquito-borne diseases.Keywords: Phytotelmata; Mosquito Borne Diseases; Mosquito Breeding Places; Mosquitoes Bite. Dalam rangka meningkatkan peran serta masyarakat dalam mencegah penyebaran penyakit tukar nyamuk itulah pendidikan kepada masyarakat perlu perlu dilakukan  melalui penyuluhan.  Kabupaten Tanggamus adalah salah satu daerah di Lampung yang kejadian DBD-nya dimana sejak Januari hingga Juni 2024 ada 227 kasus. Untuk meningkatkan pengetahuan masyarakat di Desa Banjar Agung Udik, Kecamatan Pugung, Kabupaten Tanggamus terhadap fitotelmata sebagai tempat perindukan nyamuk maka penyuluhan ini dilakukan. Penyuluhan dilakukan dengan metode pretes,  ceramah dan diskusi, serta praktikum. Hasil penyuluhan didapatkan bahwa  sebagian besar peserta tahu bahwa DBD dan malaria ditularkan nyamuk, tetapi tidak semua tahu nama spesies namuk vektornya. Peserta tahu tempat hinggap nyamuk di rumah dan juga  tahu  cara menghindarkan diri dari gigitan nyamuk. Peserta juga tahu tempat-tempat berkembang biak nyamuk, tetapi baru mengenal fitotelmata dalam penyuluhan ini. Praktikum yang dilakukan peserta berhasil mengidentifikasi enam spesies tumbuhan yang terbukti menjadi fitotelmata.Dengan demikian dapat disimpulkan bahwa penyuluhan ini berhasil memberkan pengetahuan kepada masyarakat tentang potensi fitotelmata sebagai sumber penyebar penyakit tular nyamuk.Kata kunci: Fitotelmata; Penyakit Tular Nyamuk; Tempat Perindukan Nymauk; Gigitan Nyamuk.