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Journal : Biospecies

KARAKTERISTIK DAN POLA SEBARAN PHYTOTELMATA SEBAGAI TEMPAT PERINDUKAN ALAMI NYAMUK DI KEBUN RAYA LIWA LAMPUNG BARAT Sitinjak, Mentoria Alviani; Rosa, Emantis; Agustrina, Rochmah; Sutyarso
Biospecies Vol. 13 No. 2 (2020): Juli 2020
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v13i2.9540

Abstract

Liwa Botanical Garden (KRL) is an ex-situ plant conservation area which has a large collection of plants. Therefore, there will be many types of plants in KRL that have phytotelmata. Phytotelmata is a plant in which the organs can hold water and is used by various organisms including mosquitoes as natural breeding grounds. The aim of this study was to determine the phytotelmata characteristics and phytotelmata distribution patterns in the Liwa Botanical Garden, West Lampung. This research was carried out in November 2019 - January 2020 at the Liwa Botanical Garden and the University of Lampung Botanical Gardens. The study was conducted descriptively with a survey method approach. The data obtained is displayed in tables and figures. The results showed that there were 7 types of phytotelmata in the Liwa Botanical Garden namely phytothelmata in the plants of Nepenthes mirabilis, Cyathea coopei, Bambusa sp. Areca catechu, Musa sp. Colocasia gigantea, and Crynum asiaticum with 2 phytotelmata distribution patterns: in groups with IP values ranging from 0.813-1,194 and uniform distribution patterns with IP values of -2.08.Keywords: Phytotelmata, Natural Breeding Ground, Kebun Raya Liwa.
DETEKSI KRISTAL PROTEIN PADA ISOLAT BACILLUS SP. DENGAN PEWARNAAN COOMASSIE BRILLIANT BLUE Siallagan, Maria Denada; Ekowati, Christina Nugroho; Sumardi, Sumardi; Rosa, Emantis
Biospecies Vol. 13 No. 2 (2020): Juli 2020
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v13i2.9562

Abstract

Bacillus is one of the genera of bacteria that has the potential to be developed in the field of biotechnology. Bacillus is able to produce protein crystals during the stationary phase in its life cycle. This protein crystal is a protoxin that is active will be pathogenic in insects. In this study, protein crystal staining was carried out using coomassie brilliant blue dye on the isolate of Bacillus sp. with isolate code B1, B2, and B3. Before being stained, the three isolates were bred on Nutrient Broth (NB) medium at pH 5, pH 7, and pH 9, then incubated for 72 hours. Based on research results, the three isolates were detected to produce protein crystals. Crystal protein of Bacillus sp. on media with a pH of 5 and pH 7 located at the end of the spore, while the protein crystal of Bacillus sp. isolate on media with a pH of 9 located on the outside of the spores.