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Morphological Diversity of Endophytic Bacteria Peppermint (Mentha Piperita L.) Ardiansyah, Rangga; Sapitri, Sartika Wati Dwi; Dzakwan, Muhammad Naufal; Thalib, Nisa Muhammad; Thamara, Monika; Maharani, Lia Putri; Wibowo, Risky Hadi; Sipriyadi, Sipriyadi; Darwis, Welly
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.6683

Abstract

Background: The peppermint plant (Mentha piperita L.) is a type of medicinal plant that is widely found in temperate regions, especially Europe, North America and Africa. This plant contains essential oils, menthol, and menthone, menthafuran, sesquiterpenes, triterpenes, flavonoids, carotenoids, tannins and various minerals are also widely contained in peppermint leaf oil (M. piperita L.). The purpose of this study was to isolate and determine the morphological diversity of endophytic bacteria from peppermint plants (M. piperita L.). Methodology: The method used in this study is to take isolation samples, pure culture, characterization and gram staining to determine whether it includes gram-negative or positive bacteria. The results of the study of 12 cultures of endophytic bacterial isolates from plants (M. piperita L.) were taken 10 isolates from each different culture media. Findings: The results of morphological observations of bacterial colonies show diverse characteristics in terms of surface, appearan, ceelevation, edges, and colour. Gram Staining Test of Endophytic Bacterial Isolates from the roots, leaves, and stems of mint plants against endophytic bacteria showed that there were gram-positive and gram-negative bacterial isolates. Then the results of bacterial cell shape morphology have a diversity of Cocobacil, Coccus, and Bacil forms so it can be concluded that endophytic bacterial isolates from peppermint plants (M. piperita L.) have varied morphological diversity. Contribution: Data on the morphological diversity of endophytic bacteria from M. piperita L. provides important basic data for further research on the potential of M. piperita L. endophytic bacteria
PENGAWETAN IKAN FILLET TUNA (Thunnus sp.) DENGAN KITOSAN MENGGUNAKAN METODE EDIBLE COATING Triani, Lensi Agus; Notriawan, Doni; Rahman, Refpo; Wibowo, Risky Hadi
Laboratory Journal : Jurnal Laboratorium Sains Terapan Vol. 2 No. 2 (2025): Desember
Publisher : Unib Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/jlst.2.2.33-45

Abstract

Ikan fillet tuna merupakan pengolahan inovasi ikan yang memiliki daya simpan yang singkat jika disimpan dalam suhu ruang. Penelitian ini bertujuan untuk memperpanjang daya simpan dan mempertahankan mutu fisik ikan fillet tuna dalam suhu ruangan dengan menggunakan lapisan kitosan. Penelitian ini menggunakan metode edible coating dengan merendam ikan fillet tuna pada larutan kitosan dengan berbagai variasi yakni tanpa perlakukan sebagai kontrol negatif, berbagai konsentrasi kitosan dari 1%, 1,5%, 2%, 2,5%, 3%, serta penggunaan formalin dengan konsentrasi 1% dan 10% sebagai kontrol positif selama 5 menit. Hasil menunjukkan bahwa ikan fillet tuna dengan konsentrasi 3% dapat memperpanjang daya simpan selama 4 hari sedangkan tanpa perlakuan sebagai kontrol negatif bertahan selama 2 hari, sampel dengan konsentrasi 1%, 1,5%, 2%, dan 2,5% memperpanjang daya simpan selama 3 hari. Maka dapat disimpulkan penggunaan larutan kitosan dapat digunakan sebagai pengawet ikan fillet tuna serta mempertahankan parameter fisik (tekstur, warna, aroma) dan pH ikan fillet tuna lebih baik setelah perlakukan dibandingkan sebelum perlakuan dengan larutan kitosan.
PENYULUHAN DAN PEMBUATAN PAPER SOAP (SABUN KERTAS) BAGI MASYARAKAT DI KELURAHAN PEMATANG GUBERNUR KOTA BENGKULU UNTUK MENINGKATKAN KESADARAN MENCUCI TANGAN SEBAGAI LANGKAH AWAL PENCEGAHAN WABAH COVID-19 Wibowo, Risky Hadi; Sipriyadi, Sipriyadi; Pertiwi, Reza; Hidayah, Thoriqul; Medani, Della Indah; Wulandari, Gustina Dwi; Rosianti, Nadya
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 4, No 1 (2021): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v4i1.317-324

Abstract

Hand is the main transmission pathogen entry into the body which is the part of the body that is most often in direct contact with other limbs and surrounding objects. The importance of maintaining hand hygiene by always washing hands with soap is the first step in preventing transmission of COVID-19. However, during a pandemic like this, it will be difficult to find soap available everywhere, and tends to be hard to carry all the time. Therefore, this service aims to invite the people of Pematang Gubernur, Bengkulu City, to use paper soap because it is easier to use its use and flexible to carry anywhere besides being an effort to prevent the COVID-19 outbreak, it can also add to new information and skills for residents in making attractive soaps. The feedback response given by the community service implementation team to the community service participants resulted in a positive response to this activity, who initially did not know about making and use of paper soap and became aware of this. 
Identifikasi dan Patogenisitas Agen Bakteri Penyebab Penyakit Busuk Batang pada Buah Naga (Hylocereus sp.) Fahmi, Riziq Ilham Nur; Cahlia, Uci; Wibowo, Risky Hadi; Sipriyadi, Sipriyadi
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 1 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i1.705

Abstract

The increasing intensification of dragon fruit (Hylocereus sp.) cultivation is followed by an increase in disease attacks that have the potential to reduce plant productivity. This study aims to isolate, identify, and test the pathogenicity of bacteria that cause dragon fruit stem rot in Pematang Donok Village, Kepahiang, Bengkulu. Bacterial isolation was carried out from disease-infected stem tissue, followed by isolation purification. Bacterial identification was carried out based on colony morphology and Gram staining, while pathogenicity and hemolysis tests were carried out on potatoes and blood agar media, respectively. The data were analyzed descriptively qualitatively through observation of colony morphological characteristics and Gram staining, as well as pathogenicity tests on potatoes and hemolysis tests on blood agar. The isolation results obtained two bacterial isolates, namely isolates BBNK1 and BBNK2 with different colony morphology characteristics. Both isolates are Gram-positive bacteria with bacillus and coccus cell shapes. Pathogenicity tests on potatoes showed that both isolates were able to cause soft rot symptoms such as tissue softening, discoloration, and mucus formation compared to the control. Hemolysis tests showed that only isolate BBNK1 showed hemolytic activity with the formation of a clear zone around the isolate. The findings of this study offer foundational data to support future research on molecular identification and the development of more environmentally friendly and sustainable disease management strategies.
Synthesis of Chitosan-based Materials with Ester-benzoate Modification and Its Application as An Antimicrobial Agent Ratnawati, Devi; Wibowo, Risky Hadi; Andini, Vicka; Maryanti, Evi; Kurniawan, Yehezkiel Steven
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.352

Abstract

Microbial infections pose serious threats to human health, emphasizing the need to develop effective antimicrobial materials. This study aims to synthesize, characterize, and evaluate the antimicrobial properties of ester vanillin-benzoate (1) and its chitosan-based composite material (2). Furthermore, the synthesized compound was tested for antibacterial and antifungal activities against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans using the Kirby-Bauer diffusion method. The synthesized compounds were effective against S. aureus and B. subtilis but had minimal action against P. aeruginosa, E. coli, and C. albicans. The formation of the Schiff-base composite could increase its antibacterial activity, indicating a synergistic effect arising from the combination of compound 1 and chitosan.
Co-Authors : Samwilson Slamet, : Samwilson ACENG RUYANI Adriansyah, Aulia Aini Nurul Andini, Vicka Anggraini, Putri Lisya Anisah, Saskia Anugrah, Ratu Intan Ardiansyah, Rangga Azhar, Afra Wafiqah Berutu, Ali Sadikin Berutu, Mei Awbina Darmi, Darmi Devi Ratnawati Dominica, Dwi Dzakwan, Muhammad Naufal Elfi Yuliza Ernis, Gustria Evi Maryanti Fadhila, Muhammad Adeng Fahmi, Riziq Ilham Nur Fatimatuzzahra Fatimatuzzahra Fitri, Sabrina Helmiyetti Hidayah, Thoriqul Ilfanisari, Vinolla Iman Rusmana Indah Medani, Della Jarulis Jarulis Jody, Regina Kurniawan, Yehezkiel Steven Lingga, Rahmad Maharani, Lia Putri Mashudi Mashudi Masrijal, Camelia Dwi Putri Medani, Della Indah Morina Adfa Nabella, Inastia Nabila, Bella Nasution, Riski Padilah Nesbah, Nesbah NISA RACHMANIA MUBARIK Notriawan, Doni Oky Hermansyah Prastya, M. Eka Puspytha, Yolanda Reagen, Muhamad Alvin Refpo Rahman Reza Pertiwi Rina Suthia Hayu Rindiyani, Rindiyani Rizwar, Rizwar Rofiani, Desi Rosianti, Nadya Rothman, Dita Delia Safniyeti SALPRIMA YUDHA S Salsabilla, Sisilia Sapitri, Sartika Wati Dwi Saputra, Apin Sari, Chaincin Buana Sari, Dhea Amelia Sasmita, Anggun SEMPURNA GINTING Setiawan, Redo Shufyani, Fahma Silvia, Elsi Simanjuntak, Putri Hezekiel Claracia Sinisuka, Alfredi Anis Fadhila Ginting Sipriyadi Suci Rahmawati, Suci Susanti, Elia Taifur, Aghus Thalib, Nisa Muhammad Thamara, Monika Thenawidjaya, Maggy Tri Sunardi, Tri Trianda, Aldy Triani, Lensi Agus Uci Cahlia Wahyuni, Reza Welly Darwis Wulandari, Gustina Dwi Yar Johan, Yar