Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Operations Research: International Conference Series

Identification of Pathogenic Bacteria on Carp Commodities (Cyprinus carpio) at Quality Control and Fishery Product Safety Agency (BKIPM) of Bengkulu Wibowo, Risky Hadi
Operations Research: International Conference Series Vol. 1 No. 2 (2020): Operations Research International Conference Series (ORICS), June 2020
Publisher : Indonesian Operations Research Association (IORA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47194/orics.v1i2.72

Abstract

Carp (Cyprinus carpio) is a type of freshwater fish that is widely cultivated. The increase in the amount of production and trade in freshwater fishery commodities both for consumption in Bengkulu will potentially increase the risk of entry and spread of pests and diseases in fish, which at the same time will be a threat that can endanger and damage the sustainability of fishery biological resources. Bacteria that infect fish can inhibit the expected production targets, which is an outbreak of pathogenic fish disease caused by bacteria. This study aims to identify pathogenic bacteria that infect Carp (C. carpio). Carp were obtained from fish traders at Panorama Market, Bengkulu City. Carp samples were selected based on clinical symptoms that were no longer healthy. Isolation of bacteria from Carp’s organs using Triptic Soy Agar (TSA) media. The isolates were screened by morphological characters and biochemical test. The results of this study showed that total of 2 bacteria were isolated. Based on biochemical tests carried out such as the Simmons Citrate test, Triple Sugar Iron Agar, Oxidative-Fermentative, Motility Indol Ornithine, Lysine Iron Agar, MR-VP, urea, catalase, oxidase, gelatin, confectionery test, and Rimmler-Shotts test, pathogenic isolates Sp 1. in the sample have a close relationship with Plesiomonas shigelloides while the pathogenic isolates Sp 2. and Sp 3. have a close relationship with Aeromonas hydrophila.
Antibacterial Activities of Pseudomonas orientalis APD 16 Isolate Sponge-Associated Aplysina sp. from Enggano Island Against Escherichia coli and Staphylococcus aureus Wibowo, Risky Hadi
Operations Research: International Conference Series Vol. 1 No. 2 (2020): Operations Research International Conference Series (ORICS), June 2020
Publisher : Indonesian Operations Research Association (IORA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47194/orics.v1i2.74

Abstract

Sponge is one of the invertebrates from the Porifera phylum. Sponge bodies have structural complexity with different cell layers. The sponge has many pores (ostium) on the surface of their body as a filter feeder. Sponge is recognized as organisms that have the potential because they can produce metabolites. Secondary metabolites produced by sponges are the result of the association of sponges with bacteria. The sponge used in this study is the Aplysina sp. sponge collected from Enggano island, Bengkulu Province. Aplysina sp. sponge is known to contain metabolites with antibacterial, antifungal and cytotoxic activity on cancer cells. This study aims to identify of potential isolate associated with Aplysina sp. sponge collected from Enggano island. Isolation of bacteria from Aplysina sp. sponge using Sea Water Complete (SWC) media. The isolate was screened by antagonistic test, morphological characters, Gram-staining, biochemical test and molecular identification. Based on the antagonistic test, APD 16 isolate could inhibit Escherichia coli and Staphylococcus aureus in Vitro. APD 16 isolate was identified molecularly using of 16S rRNAgenes analysis and it genetically close with Pseudomonas orientalis.