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Sri Rezeki
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Journal : Jurnal Ilmiah Mahasiswa Veteriner

 1 
IDENTIFIKASI DAN KARAKTERISASI GEN CALPAIN (CAPN1) PADA KAMBING KACANG (Identification and Characterization of the Calpain Gene (CAPN1) in Kacang Goat sri rezeki; T Zahrial Helmi; Herrialfian Herrialfian; M Hasan; M Jalaluddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 3, No 4 (2019): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v3i4.7829

Abstract

Penelitian ini bertujuan untuk mengetahui keberadaan dan karakteristik gen calpain untuk perbaikan mutu genetik pada kambing kacang (Capra hircus). Sampel penelitian yang digunakan adalah darah yang diambil dari 5 ekor kambing kacang yang dipelihara di UPT Hewan Coba FKH Unsyiah Banda Aceh. Tahap pertama dilakukan isolasi DNA dari darah, tahap kedua dilakukan elektroforesis, hasil ekstraksi kemudian dilanjutkan dengan optimasi suhu annealing, amplifikasi dan sekuensing. Hasil sekuensing dianalisis menggunakan software program Basic Local Alignment Search Tool (BLAST) dan Clustal W dari program Mega 6.01. Hasil optimasi suhu annealing diperoleh suhu 56°C merupakan suhu yang tepat untuk melakukan amplifikasi. Semua sampel yang diteliti amplifikasi gen calpain diperoleh hasil sepanjang 709 bp sesuai dengan primer yang digunakan. Hasil analisis program BLAST dan program Mega 6.01. menunjukkan kekerabatan yang sangat dekat, ditandai dengan berada pada satu cabang dari pohon filogenetik dan hasil urutan gen calpain (CAPN1) kambing kacang (Capra hircus) memiliki kedekatan dengan gen calpain Capra hircus breed Barbari sebesar 98%. Hasil penelitian ini dapat disimpulkan bahwa gen calpain pada kambing kacang yang teramplifikasi sepanjang 709 bp, dan mempunyai kedekatan sekuen nukleotida dengan gen calpain pada beberapa jenis kambing lainnya dan dapat digunakan untuk perbaikan mutu genetik kambing kacang.(The aim of this research was to find out the presence and characteristics of calpain genes for the improvement of genetic quality in kacang goat (Capra hircus). The sample for research using blood taken from 5 Capra hircus which kept in UPT Animal Laboratory of FKH Unsyiah, Banda Aceh. The first stage is DNA isolation from blood, the second stage is electrophoresis, the extraction then continued with optimization of annealing temperature, amplification and sequencing. The results of sequencing analyzed using software program named Basic Local Alignment Search Tool (BLAST) and Clustal W from program Mega 6.01. Annealing temperature optimization results obtained temperature of 56 ° C is the proper temperature to do amplification. All samples studied with calpain gene amplification gained results of 709 bp according to the primary used. Results of BLAST program analysis and Mega 6.01 program. shows a very close genetic relationship, characterized by being on the same branch of phylogenetic tree and the result sequence of  calpain gene (CAPN1) of the goat (Capra hircus) possessing proximity to the calpain genes Capra hircus breed of Barbari by 98%. The results of this study can be concluded that the calpain gene in Capra hircus is amplified by 709 bp, and has the adjacency of nucleotide sequence with the calpain gene in some other types of goats and can be used as a genetic improvement for Capra hircus).
Co-Authors Ade Rachmat Yudiyanto Ade Rachmatb Yudianto Afrizal Agus Rahmadsyah Agustina Alifia Restu Selvanda Amelia Erawaty Siregar Amellia Erawaty Siregar Andrian, Dedek Anisa Malfiro Antonio Lodian Apriyoga Sukamto Aras Mulyadi Aris Munandar Asianna Martini Simarmata Associate Prof. Dr. Muhammad Hafiz Abd Rashid Astaria Br Ginting Aulia Iski Bayu Jaya Tama Bella Maryati Bey Todu Br.Manjorang, Novita Andriani C Putroe Ula Rahifa Cecilia Chendy Meylia Cut Reinilda Rischa Cut Zahri Harun Damayanty S Dara Mauliza Dea Agustina dedi irawan Delpi Novianti Desma Erica Maryati Manik Dewi Saputri Dian Setyorini Dr Asliza Yusof Effendi, Leo Adhar Eka Purnamasari Erin Padilla Siregar Esty Rahayu Fachry Abda El Rahman Fajri Ekawardana Fajrina Mazaya Femilina Nazara Fitri Mayasari Panggabean Fitriana Yolanda Gusman Lesmana Gustina Harum Sasanti Hazizah Sabilah Siagian Henni Jc Saragih Herrialfian Herrialfian Ihdina Gustina Ilham Muhammad Ishafit Ishafit Isti Mulyati Istikomah, Endang Jhona Boy Erwinta Sembiring Kenny Kiki Khoiriyani Kiki Wahyuni Lasria Yolivia Aruan Liandy, Nicholas Lie, Liandro Lina Linda Wahyu Marpaung Lisdayanti Simanjuntak Lubis , Ayu Ulfah Nur M Hasan M Jalaluddin Masayu Hamdatul Azizah Luthfiyyah Megawati Saragih Mei Tri Janti Silalahi Melly Sukma Dewi Ratu Mutriara Mely Sukma Dewi Ratu Mutiara Merlin Qhardanova Merry Susanti Mesrida Simarmat Michael Muammar Rinaldi Muchlas Muchlas Muhammad Ali Akbar Muhammad Rizki Bima Putra Murniati Ar Nadia Jubta Nainggolan, Elisabeth Narti Prihartini Ngurah Ray Nizam Azmal Abdul Malek Nopriadi Nopriadi Nunung Faujiah Nur Azlina Nurhajimah Nurhajimah Nurlayli Istiqomah Nurul Elenawati Nurul Huda Nurul Mustabsyirah Rafi'i Nurwani Nurwani Ong, Virginia Parningotan Simanjuntak Petrus Loo Pipin Kurnia Plora Novita Sinaga Prasetyo, Stephanie Pristiyono puspitasari, angelin Putri, Serly Rachmi Fanani Hakim Rahmawati Yuliyani Ratri Tri Hapsari Ratu Syafira Nurasyifa Ridha Andayani Ridho Wahyuni Rika Marsulina Pandiangan Rinaldo, Rinaldo Risa Rahma Putri Rizky Darmawan Rohana Fika SKD Salma Salma Santi Chismirina Say Gumira Seri Wahyuni Harahap Silaban, Marta Armita Silvia Desmara Steven Alfando subanar subanar Sunnati , Sunnati . Sunnati Sunnati Suripah . Susda Heleni Syifa . T Zahrial Helmi Tasya Lamtiur Simanjuntak Tesalonika Simalango Tri Husni Ulis Mayrosa Ulkharimah Uray Heri Mulyanto Uza Kristiyanto Valentina Sari Theresia Siagian Vanessa Vanie Wijaya Vinna Angelia Widya Anggriani Putri Winny Wulandari Pratiwi Wirna Parida yahfizham yahfizham Zafrullah Zafrullah Zetriuslita Zulfa Rusiwan Zulfan M ALIBASYAH Zulfan M. Alibasyah Zulkarnain ' Zuraida Usman Bany Zyad Dilmi