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Mutaqin, Zaenal
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Journal : Protech Biosystems Journal

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Optimization of Anther Cultur for Pepper (Piper nigrum L.) as an Initial Stage in the Biotechnology Process of Superior Seedling Propagation Yunita, Tika Rahma; Muliani, Muliani; Mutaqin, Zaenal
Protech Biosystems Journal Vol 5, No 1 (2025): Protech Biosystem Journal
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/protech.v5i1.32198

Abstract

One of breeding activities aimed at increasing the genetic diversity of pepper plants is through anther culture. This study aims to evaluate combination of MS media supplemented with hormones for callus formation from pepper anther explants. The method used was a factorial Completely Randomized Design (CRD), with the first factor being three media compositions: full-strength MS, ½ MS, and ¼ MS; and the second factor being five combinations of hormone, namely: A. 0 ppm NAA : 1 ppm BAP; B. 1 ppm NAA : 0 ppm BAP; C. 1 ppm NAA : 1 ppm BAP; D. 2 ppm NAA : 1 ppm BAP; and E. 1 ppm NAA : 2 ppm BAP. The results of this research are a significant contribution to the development of tissue culture-based bioprocess system for producing superior pepper seedling through anther culture technology. The application of ¼ MS medium supplemented with 2 ppm NAA and 1 ppm BAP induced callus formation at a rate of 10%.
Co-Authors Abdurahman Abdurrahman Achmad, Dwi Isyana Ade Hidayat, Ade Amalia, Kaniati Amrozi Khamidi Ardianti, Nova Asep Nurjamin Danie Indra Yama Delyani, Rista Didin Sahidin Eso Solihin Fakhrudin, Jaini FF, Vera Hananda Priyandaru HARYADI, ARDI MULYANA Hidayat, Danil Afif Ida Ayu Suci Indrawan, Yuni ISNAENI, NUR Kushardiyanti, Dessy Linda Linda Maulana, Haris MUHAMAD SHOLEH Muhamad Tabrani, Muhamad MUHAMMAD ALI MUHAMMAD RIZAL Muliani Muliani Muliani Muliani, Muliani Muryati Muryati Naturindo, Naupal Newis, Yumma Nurakhim, Bambang Okto Ivansyah Purwandani, Ledy Rahayu, Dian Yuniar Syanti Rifqi Aulia Erlangga Ririn Febriyanti Rukman Rukman Sahlani, Sahlani Sandrawati, Apong Selvi Pratiwi Sinaga, Yusmarida Suhardi Suhardi Susana Susana Tamansyah, Gustya Ubaidah, Siti Widyastuti, Ragil Putri Winnugroho Wiratman, Manfaluthy Hakim, Tiara Aninditha, Aru W. Sudoyo, Joedo Prihartono Y. Erning Indrastuti YATIM RIYANTO YAYAH NURHIDAYAH, YAYAH Yunita, Tika Rahma Yusro Zikrillah, Abdu