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All Journal Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease Jurnal Medik Veteriner Jurnal Abdi Insani Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat LOSARI: Jurnal Pengabdian Kepada Masyarakat journal of Basic Medical Veterinary Indonesian Journal of Innovation Studies Jurnal Vokasi Indonesia Jurnal Agro Veteriner
Sabdoningrum, Emy Koestanti
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Journal : Jurnal Agro Veteriner

 1 
Milk quality test using lactose, specific gravity and fat test approaches at the Kertajaya Village Cooperative Unit, Kediri Astuti, Elita Sriwidya; Effendi, Mustofa Helmi; Rahmahani, Jola; Suwarno, Suwarno; Sabdoningrum, Emy Koestanti; Tacharina, Martia Rani
Jurnal Agro Veteriner Vol. 8 No. 2 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i2.74045

Abstract

The quality of fresh milk based on SNI 2011 regarding fresh milk requirements includes physical, chemical and the number of microorganisms. One of the physical properties of milk can be seen from the density test. The chemical properties of milk can be seen from the lactose and fat rate. The lactose test is by means of titration of the filtrate resulting from filtering milk which is free from any content other than lactose. Density test using a lactodensimeter. Fat test using the Gerber method. From the results of research on the quality of cow's milk which was carried out using the lactose test approach, density and fat obtain average results of 3.98% of lactose rate, 1.0259 g/ml of density and 2.7% of the at rate in VUC Kertajaya is good and close to the criteria according to SNI 2011. This can affect the selling price of milk from farmers in VUC Kertajaya.
Detection of Salmonella sp. in broiler chickens in closed houses using the polymerase chain reaction method Larasati, Anissa Dhea; Sabdoningrum, Emy Koestanti; Nidom, Chairul Anwar; Hidanah, Sri; Rachmawati, Kadek; Puspitasari, Yulianna
Jurnal Agro Veteriner Vol. 9 No. 1 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v9i1.78646

Abstract

This study aimed to detect the presence of Salmonella sp. in broiler chickens raised in closed house systems using the Polymerase Chain Reaction (PCR) method. Liver and intestinal samples were collected from chickens showing clinical signs such as diarrhea, anorexia, and lethargy, along with pathological lesions observed during necropsy. Bacteriological identification involved isolation using selective media, Gram staining and biochemical tests. Two samples (3A and 3B) showed colony morphology and biochemical characteristics consistent with Salmonella sp. Confirmation using PCR targeting the invA gene (primers 139 and 141, expected amplicon size 284 bp) yielded negative results in both samples, while the positive control successfully amplified the target. The inconsistency between bacteriological and molecular results may be attributed to several factors: absence or mutation of the invA gene in the tested isolates, primer mismatch with local Salmonella strains, or inadequate DNA quality and concentration. These findings suggest that reliance on a single molecular marker may be insufficient for accurate detection and underscore the importance of optimizing PCR conditions. The study highlights the need for locally adapted primers and complementary diagnostic approaches to improve the reliability of Salmonella sp. detection in poultry, particularly in intensive production systems like closed house environments.
Co-Authors Al arif, Mohammad Anam Al-Arief, Moh. Anam Al-Arif , M. Anam Al-Arif, Anam Astuti, Elita Sriwidya Azizah, Nanda Kholifatul Benjamin Christoffel Tehupuring, Benjamin Christoffel Budi Utomo Chusniati, Sri Dadik Rahardjo, Dadik de Vries, Garry Cores Dewi, Arini Rahmi Eka Pramyrtha Hestianah, Eka Pramyrtha Erma Safitri Fatin Fadhilah Hasib Hadiah Fitriyah Hasib, Fatin Hendarti, Gracia Angelina Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri Hidayatik, Nanik Ira Sari Yudaniayanti Irawan, Adinda Riezki Ismudiono Ismudiono, Ismudiono Kadek Rachmawati Khairullah, Aswin Rafif Larasati, Anissa Dhea Lokapirnasari, Widya Paramitha Lovela, Aprinda Ratna Miarsono, Sigit Mirni Lamid Mufasirin Mustofa Helmi Effendi Nayan, Nazri Nidom, Chairul Anwar Novianti, Arindita Niatazya Nurliyani Nurliyani Rahmahani, Jola Rahmawati, Kadek Rochmah Kurnijasanti Rosyada, Zulfi Nur Amrina Safitri, Erma - Sarmanu, Sarmanu Soeharsono Soeharsono Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Supranianondo, Koesnoto Supranianondo, Kusnoto Suwarno Suwarno Swari, Wahyu Retno Tacharina, Martia Rani Wahjuni, Retno Sri Wahyuni, Retno Sri Warsito, Sunaryo Hadi Widodo, Oky Setyo Widya Paramita Lokapirnasari Wurlina, W Yudanianyi, Ira Sari Yudaniayanti, Ira Yulianna Puspitasari ZULFAH