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Penggunaan Probiotik Alami Berbahan Rempah pada Tambak Tradisional Blandongan, Kota Pasuruan Kartikaningsih, Hartati; Koentjoro, Maharani Pertiwi; Sudaryanti, Sri; Anggraeni, Ike Listya; Gunawan, Fahdynia Karnira; Ashari, Fadhil Muhamad
Jurnal Gramaswara: Jurnal Pengabdian kepada Masyarakat Vol. 5 No. 2 (2025): Jurnal Gramaswara: Jurnal Pengabdian kepada Masyarakat
Publisher : Faculty of Cultural Studies, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.gramaswara.2025.005.02.11

Abstract

Traditional farmers in Pasuruan City face challenges in increasing pond productivity due to declining land quality and reliance on natural feed. Milkfish growth is often hampered by lack of feed and increased disease due to environmental changes. Although manufactured feed and probiotics are available, they are too expensive for traditional farmers. Pasuruan City has great potential for the development of milkfish farming, and the Banyu Urip Fish Farmers Group (POKDAKAN) is an experienced community in this field. The use of probiotics made from spices, such as ginger, turmeric, and curcuma, is proposed as an eco-friendly and economical solution as these ingredients are abundant and effective in improving pond water quality and fish disease resistance. The results of probiotic application showed significant improvement, with 85% of farmers reporting up to 30% increase in production, and 90% recording a decrease in fish disease cases. Pond water quality also improved, particularly in ammonia and dissolved oxygen levels. The use of this natural probiotic not only positively impacts the pond environment, but also supports the sustainability of milkfish farming productivity in Pasuruan City.
Efikasi Oxytetracycline Terhadap Kesehatan Ikan Lele yang Diinfeksi Bakteri Edwardsiella tarda A'yunin, Qurrota; Kartikaningsih, Hartati; Andayani, Sri; Arifin, Nasrullah Bai
JFMR (Journal of Fisheries and Marine Research) Vol. 3 No. 1 (2019): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2019.003.01.15

Abstract

Edwardsielliosis is a disease that commonly infects freshwater fish including catfish (Clarias sp). Edwardsielliosis is caused by Edwardsiella tarda infection and causes mass mortality in catfish farming. Overcoming diseases in freshwater fish can use drugs or antibiotics that are classified as safe and their use is in accordance with government regulations. This study aims to determine the appropriate dosage of Oxytetracycline in catfish infected by E. tarda bacteria through histological analysis. The study design used Completely Randomized Design with 3 treatments, 1 control and 3 replications in each treatment. Catfish used were ± 25 cm in size and the dose of oxytetracycline used were 15, 30, and 45 ppm. The results showed tissue damage edema, vacuolization, necrosis, and haemorhage in fish infected with Edwardsiella tarda bacteria with the lowest damage at 45 ppm of oxytetracycline treatment. The results indicate that oxytetracycline has an effect on muscle histology and recovery of fish health.
Antibakteri Ekstrak Etanol Serbuk Kering Sargassum cristaefolium Terhadap Bakteri Escherischia coli dan Salmonella thyposa Kartikaningsih, Hartati
JFMR (Journal of Fisheries and Marine Research) Vol. 4 No. 1 (2020): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2020.004.01.8

Abstract

Sargassum cristaefolium dried powder can be used as food and fed additives as well as  a beverage powder, contained some minerals and terpenoid active ingredients. This study aimed to determine the anti-bacterial activity of Sargassum cristaefolium dried powder on the inhibition of Escherischia coli and Salmonella thyposa bacteria in vitro. The research showed that the dried powder of Sargassum cristaefolium had better inhibition on Salmonella thyposa (2.3 mm) compared to Escherischia coli (1.2 mm) although it was classified as weak bactericidal. Sargassum cristaefolium dried powder showed positive testing for alkaloids, flavonoids, terpenoids and tannins and does not contain saponins, had low toxicity (LC50 322 ppm). The LCMS test showed a peak at 2.16 retention time with a molecular weight of 339.39 m/z. The structure of the Sargassum cristaefolium antibacterial active ingredient should be clarified using C-NMR and H-NMR.
Evaluation of the Role of Community Health Center Policies in the Handling and Prevention of Tuberculosis in Malang City Afifa, Faisa; Sholihah, Qomariyatus; Kartikaningsih, Hartati
Wacana Journal of Social and Humanity Studies Vol. 28 No. 3 (2025): WACANA, Jurnal Sosial dan Humaniora
Publisher : Sekolah Pascasarjana Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.wacana.2025.028.03.01

Abstract

Tuberculosis (TB) remains a significant public health issue in Indonesia, including in Malang City. Despite various strategies, the disease poses significant challenges due to social, economic, and environmental factors. This study aims to evaluate the role of Community Health Center (Puskesmas) policies in TB management and prevention efforts in Malang City while identifying factors that influence program success. A descriptive quantitative method was employed, utilizing Structural Equation Modeling (SEM), SWOT analysis, and the Analytic Hierarchy Process (AHP). Primary data were collected through questionnaires and interviews with TB patients, health workers, and the community, while secondary data were obtained from local health office reports. The findings revealed that implementing Puskesmas policies has generally been effective, particularly regarding mandatory reporting, active case finding, and free TB treatment. However, several barriers persist, including inconsistent treatment adherence, stigma surrounding TB, inadequate living conditions, and limited healthcare access in certain areas. SEM analysis identified key factors affecting TB control success: patient knowledge, environmental conditions, treatment experience, and the role of health workers. SWOT analysis highlighted strengths such as strong community engagement and policy frameworks, alongside weaknesses like insufficient environmental interventions and healthcare resource limitations. Optimization strategies proposed include enhancing public education on TB prevention, improving the home environment to support recovery, strengthening health worker capacity, integrating digital technologies, and fostering multi-sector collaboration. The study emphasizes the need to address biomedical and socio-environmental determinants for effective TB control. These findings provide strategic recommendations for enhancing Puskesmas policy implementation toward Indonesia’s 2030 TB elimination goals. Keywords: tuberculosis, community health center policy, environmental factors, public knowledge, strategy optimization
Pengembangan Protokol Deteksi Staphylococcus aureus Berbasis Molekuler Koentjoro, Maharani Pertiwi; Alviani, Melinda Nuril; Jatmiko, Yoga Dwi; Habibah, Laila Nur; Al Fatih, Ahmad Nuril Fuad; Kartikaningsih, Hartati
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9494

Abstract

Staphylococcus aureus becomes a normal flora in humans, especially on the skin and in the nose. However, if it becomes excessive or if there are pathogenic variants, it can cause various health problems. The purpose of the study is to develop a molecular-based detection method for Staphylococcus aureus using the norA primer gene. The norA gene in Staphylococcus aureus is known to play a role in pathogenesis with its antibiotic resistance ability. This type of research is analytical observational with a cross-sectional method. The methods in the study include the isolation and identification of Staphylococcus aureus from healthcare facility equipment. Isolation and identification include bacterial isolation using Blood Agar Plate (BAP) media; isolate purification, Gram staining; biochemical tests using Mannitol Salt Agar (MSA) media, glucose tests, Voges Proskauer (VP) tests, catalase tests, and coagulase tests. Furthermore, S. aureus isolates were tested using a molecular-based method, namely Polymerase Chain Reaction (PCR). This method includes DNA isolation stages, qualitative testing with agarose gel electrophoresis, semi-quantitative testing with image J software, amplification with Real-Time Polymerase Chain Reaction (RT-PCR) using norA gene primers. The Mann-Whitney test results gave a value of p = 0.334 (p> 0.05) indicating the suitability between the culture method and the PCR method with the developed protocol in detecting Staphylococcus aureus. The developed method includes the use of base sequences in the norA gene primer, optimization of annealing and extension temperatures, and the concentration of DNA templates used.
Improving Nutritional Profile and Consumer Acceptance of Siomay Stuffing Through Integration of Mackerel Meat (Rastrelliger SP.) and Yellow Potato Flour (Solanum Tuberosum L.) Sukoso, Sukoso; Kartikaningsih, Hartati; Ma’rifat, Tian Nur; Alif, Muhammad Rizki Mas
Jurnal Indonesia Sosial Sains Vol. 5 No. 08 (2024): Jurnal Indonesia Sosial Sains
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jiss.v5i08.1225

Abstract

Siomay, a popular Indonesian snack traditionally made with high-fat chicken or beef fillings, can be made healthier by using mackerel fish instead. This modification, combined with the addition of potato starch, aims to improve the nutritional profile of siomay while maintaining its appeal to consumers, as mackerel is both tasty and nutritious.  This study aims to improve the nutritional profile and consumer acceptance of siomay filling through the integration of mackerel meat and potato starch. Data were analyzed using a Completely Randomized Factorial Design with two factors (mackerel meat and potato starch) mixed in different proportions in the siomay filling dough. Parameters tested included nutritional composition (protein, fat, carbohydrate, fiber, and mineral), physicochemical properties (texture, color, and aroma), and consumer acceptance test using a hedonic scale. The results showed that the most preferred treatment was K2D2 (14g mackerel meat and 20g potato starch), which was 5.6±1.5. The nutritional content of the siomay filling preferred by the panelists increased (water 29.07 g, protein 12.89 g, fat 3.65 g, ash 3.41 g, sugar 1.35 mg, vitamin B1 0.26 mg). However, the carbohydrate content decreased to 50.98 g, compared to the content of siomay filling without the addition of yellow potato starch (56, 74 g). This study shows that mackerel flour and potato starch can potentially be used to improve the nutritional profile and consumer acceptance of siomay filling.
A Analysis of Land Use on Water Quality in Merjosari Village, Lowokwaru Subdistrict, Malang City K, Maharani Pertiwi; Anshary, Muhammad Reza; Kartikaningsih, Hartati; Fadila, Rizki
Gema Lingkungan Kesehatan Vol. 23 No. 4 (2025): Gema Lingkungan Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/gelinkes.v23i4.343

Abstract

Malang City faces several environmental challenges, including inadequate sanitation and water management, which have not been fully addressed across all aspects of life. This is not in line with the Sustainable Development Goals, which aim to ensure the availability and sustainable management of clean water and sanitation for all people. Malang City is also experiencing increasingly massive land use changes, from green open land to housing, which can impact clean water quality. This study aims to assess the quality of clean water from the non-Local Water Supply Utility in Merjosari Village, Lowokwaru Subdistrict, Malang City, which is undergoing land-use transformation. A quantitative observational approach was employed using the National Sanitation Foundation's Water Quality Index (NSF-WQI) method, which evaluates water quality based on physical, chemical, and biological parameters. The results showed that the water quality index in Merjosari Village, Lowokwaru District, Malang City, analyzed using the NSP-WQI method, has a value of 77.53 and falls within the good criteria. However, several parameter values exceed the clean water standard. The average value of the DO parameter, at 5.85 mg/,L is less than the quality standard of 6 mg/L. The average value of the BOD parameter, at 8.12 mg/,L exceeds the quality standard of 2 mg/L. The pH parameter at several sample points exceeds the quality standard of above 8.5, and the Nitrate Parameter at several sample points exceeds the quality standard of 20 mg/L. In conclusion, the land cover change in the Merjosari area, Lowokwaru District, Malang City, does not significantly affect the quality of clean water. However, the values of several water parameters exceed the clean water standard, which has the potential to impact public health negatively.
Plankton Diversity as a Natural Food Source for Milkfish (Chanos chanos) in Traditional Ponds of Pasuruan City, East Java Ike Listya Anggraeni; Hartati Kartikaningsih; Agung Pramana Warih Marhendra
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.7738

Abstract

Traditional ponds in Pasuruan City are dominated by milkfish (Chanos chanos). Milkfish take food from the top layer of the seabed in the form of phytoplankton and zooplankton. This study aims to determine the abundance, diversity and dominance of plankton and water quality in traditional milkfish ponds in Pasuruan City. Sampling locations were selected based on the distance of the pond from the sea. Station 1 (near the sea), station 2 (1–3 km from the sea), and station 3 (furthest from the sea). The results showed the availability of natural food for milkfish in the form of phytoplankton and zooplankton. Phytoplankton was found more than zooplankton. The most common phytoplankton division was Bacillariophyta and the least was Euglenozoa with diversity index ranging from 1.64–1.82 and dominance index ranging from 0.24–0.31. The most common zooplankton phylum is Arthropoda and the least is Molluscs with diversity index ranging from 0–1.83 and dominance index ranging from 0.21–1.00. The measurement results of pond water quality parameters such as pH (8.51–9.02), temperature (30.1–32.5°C), brightness (25.5–29.6 cm), DO (3.7–5.7 ppm) and salinity (32.5–33.7 ppt) are still in accordance with the optimal standards of milkfish farming.
Assessment on Medowo Village Kandangan District, Kediri Regency as Biogas Based Energy Independent Village Wahyu Devi Hapsari Wijayanti; Surjono; Hartati Kartikaningsih
Jurnal Penelitian Pendidikan IPA Vol 11 No 4 (2025): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i4.10172

Abstract

National energy security can be achieved by improving energy security at the village scale. Village energy independence can be achieved if at least 60% of the village's energy needs can be met by utilizing local energy potential. To determine the energy independence of a village, it is necessary to collect data on total energy usage and the potential generation of existing energy source. Medowo Village was chosen as a case study because this village actively use biogas as energy source for cooking. This study was conducted using a mixed method approach with an explanatory sequential design type. The descriptive method is used to describe the condition of energy supply in Medowo Village. The quantitative method is used to determine the condition of supply-demand and the potential for biogas generation. Primary data collection was carried out through questionnaires and direct observation at the location. Meanwhile, secondary data collection was obtained from literature studies, KUD Kertajaya, and related government agencies. It was found from the study that current biogas production only reach 40.31% of the theoretical biogas production potential. As for total energy mix, biogas contribution is 15,37%. Therefore alternatives are offered to achieve energy independence by paying attention to problems encountered during research.
A Solid Waste Pond Tiger Shrimp (Peneaus monodon) as Fertilizer for Caulerpa lentillifera Saputra, Nyoman Robby Manik; Sukoso, Sukoso; Kartikaningsih, Hartati
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1003.384 KB) | DOI: 10.21776/ub.jels.2016.007.01.04

Abstract

Farming the shrimp, fish and another commodity could produce large quantities of waste. Aquaculture waste can be formed as feces, residual feed and dead organism which are accumulated in a cultivation area. Generally, the waste is discharged directly into the water without filtration. Thus, one of the problems that are often faced by farmer is the low quality of pure water due to the high content of nutrients. Moreover, suspended solid pollutant will be formed that lead to eutrophication, oxygen depletion, and precipitation. The aim of this research is to analyze the solid waste pond tiger shrimp as fertilizer for the growth of Caulerpa lentiilifera. Here, the Random Design complete (RAL) and three times in Deuteronomy are used in the experiment. The parameters of one control and three treatment doses are used 0, 2, 4, and 6 g.L-1 respectively. The results showed that solid waste shrimp ponds can be used as fertilizer to meet the needs of Caulerpa lentillifera for growth and the results show the highest value is found in the dose of 6 g.L-1 with NO3- (4.58 ppm), NH4+ (3.34 ppm), PO43-  (2.03 ppm) and the value of the rate of growth and the PH are obtained (3.64 g.day-1) and (6,4-8), respectively. Keywords: Caulerpa lentillifera, growth rate, Nitrification, solid waste, Tiger Shrimp.
Co-Authors A'yunin, Qurrota A'yunin, Qurrota Abdul Aziz Jaziri Abdul Mulki Purnama Afifa, Faisa Agung Pramana Warih Marhendra Agung Pramana Warih Marhendra, Agung Pramana Warih Agus Susilo Agus Susilo Ahmad, Mirza Gulam Ahmad, Nurul Hawa Al Fatih, Ahmad Nuril Fuad Alif, Muhammad Rizki Mas Alifatul Fatchiyah Alviani, Melinda Nuril Amilis Setyandhinavia Andi Kurniawan Anggraeni, Ike Listya Anies Chamidah Anik M. Hariati Anshary, Muhammad Reza Apsari, Selfi'un Linda Arifin, Nasrullah Bai Arifin, Sunanto Arning Wilujeng Ekawati Asep Awaludin Prihanto Ashari, Fadhil Muhamad Atikawati, Dini Audina, Nanda Bagyo Yanuwiadi Bambang Semedi Budi Wahono Citra Satrya Utama Dewi Darius Darius Defri Yona Desy Arisandi Dewa Gede Raka Wiadnya Dian Wahyu Wardani Dini Atikawati Djamaludin, Heder Eddy Suprayitno Fauziyah, Jihan Nur Febrian Salim Prasetyo Feni Iranawati Feni Iranawati, Feni Firdaus, Shelina Innasa Fitri Candra Wardana Fuad, Mochamad Arif Zainul Galih Nugroho Gilang Rusrita Aida Gunawan, Fahdynia Karnira Habibah, Laila Nur Happy Nursyam Hardoko Hardoko Heder Djamaludin Ike Listya Anggraeni Indah Ashlachal Ummah Jeny Ernawati Tambunan K, Maharani Pertiwi Kasitowati, Rarasrumdyah Ken Audia Pradarameswari Khotibul Umam Al Awwaly Khusaini, Mohammad Kristama, Yoga Sita Kristin Rosalina Kristina Marsela Kuncoroyekti, Andre Ledhyane Ika Harlyan Lestariadi, Riski Agung Lina Asmara Wati Lina Asmarawat Luchman Hakim M. Choirul Anam Madyasta Anggana Rarassari Maharani Pertiwi Koentjoro Maharani, Sarastria Mimit - Primyastanto Mirna Zena Tuarita Mirza Gulam Ahmad Mochamad Arif Zainul Fuad Mufidah Afiyanti Mufti, Eka Deviana Muhamad Firdaus Muhamad Khafid Rifai Muhammad Rizki Mas Alif Mulabbi Andrew Nasrullah Bai Arifin Nike Poerbyanti Nurhanif, Ardhian Eko Nurul Badriyah Nurul Catur Febriana Oktavia, Yoan Pertiwi, Maharani Putra, Erwansyah Ilham Pradana Qomariyatus Sholihah Rachman Hartono Ramli, Nurul Shazini Rhytia Ayu Christianty Putri Rifai, Muhamad Khafid Rika Kurniaty Rizdya Oktofani Rizki Fadila, Rizki Roosa, Lucy Cynthia Salimatul Muntafi’ah Saputra, Nyoman Robby Manik Sari, Safitri Permata Sartika, Fitarina Setyo Tri Wahyudi Soemarno Soemarno Sri Andayani Sri Andayani Sri Sudaryanti, Sri Subagyo Subagyo Sukoso Sukoso Supirman, S S Supriyadi Supriyadi Surjono Susanto, Dony Tri Syarifah Hikmah Julinda Sari Tambunan, Jenny Ernawati Tambunan, Jeny Ernawati Tian Nur Ma’rifat Toat, Akhmad Prasetyo Ibnu Wahyu Devi Hapsari Wijayanti Wardah, Nihaya Nailatul Wiadnya D.G R Wresti L. Anggayasti Yahya Yahya Yanti Suryanti Yanuar, Adi Tiya YOGA DWI JATMIKO Yunita Eka Puspitasari Zaelanie, K Kartini Zaelanie, K Kartinie