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All Journal HAYATI Journal of Biosciences VALENSI Jurnal Fisika Unand Indonesian Journal of Pharmaceutical Science and Technology Journal of Civic Education Journal of Health Sciences Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Jurnal Informasi dan Teknologi utile: Jurnal Kependidikan JSMARTech : Journal of Smart Bioprospecting and Technology Jurnal Kesehatan Tambusai Jurnal Pengabdian Masyarakat Indonesia Jurnal SAINTIKOM (Jurnal Sains Manajemen Informatika dan Komputer) Jurnal Neuroanestesi Indonesia Journal of Applied Management Research Eduvest - Journal of Universal Studies Journal of Research and Publication Innovation Ranah Research : Journal of Multidisciplinary Research and Development Jurnal Penelitian Ilmu komputer Journal of Pubnursing Sciences Jurnal Ilmiah Research Student Jurnal Manajemen Informatika, Sistem Informasi dan Teknologi Komputer (JUMISTIK) Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Wicara Desa Jurnal Sinergi Manajemen Jurnal Bioteknologi & Biosains Indonesia
Maulana, Irvan
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Efficacy Test of Prototype Kit for Detection Bacillus cereus and Listeria monocytogenes in Processed Meat using Real-time PCR Method Nurjayadi, Muktiningsih; Fahriza, Tiara; Putri, Adinda Myra Amalia; Rahmawati, Atikah Nur; Berkahingrum, Ayu; Anggraeni, Rosita Gio; Putri, Gladys Indira; Declan, Jefferson Lynford; Akbar, Dandy; Maulana, Irvan; Azzahra, Maharanianska; Shangkara, Muhammad Arkent; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Rahayu, Sri; Saamia, Vira; Wiranatha, I Made; Abumoelak, Bassam; El Enshasy, Hesham
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 11, No. 1, May 2025
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.44280

Abstract

According to the World Health Organization (WHO), harmful agents such as Bacillus cereus and Listeria monocytogenes are responsible for 600 million cases of disease and 420,000 deaths annually. This research aims to test the effectiveness of the real-time PCR method for developing a prototype kit to detect pathogenic bacteria in processed meat. As a comparison, the and conventional PCR methods were used to obtain the accuracy, specificity, sensitivity, and effectiveness of the real-time PCR method. All the samples were cultured in solid media agar, performed amplification using specific primers cyt-K 2 and hly using PCR and real-time PCR. Meatballs, nuggets, and sausages, five samples each, were found to be positive positively contaminated with all targeted bacteria. However, they did not provide specific results using solid media culture and the PCR method. In addition, the real-time PCR method using prototype kit formulas accomplished that all contaminated samples had a Ct value smaller than the negative control, NTC (No Template Control), and had a similar melting curve to the positive control. This establishes that the real-time PCR method clarifies that all samples were contaminated with target bacteria. A formula was developed in the prototype kit with real-time PCR methods that can be used and applied on a commercial scale efficiently and precisely.
Co-Authors Abomoelak, Bassam Abumoelak, Bassam ahdaa, Adviansah Akbar, Dandy Al Rafni Anggraeni, Rosita Gio Anita Anita Anton Yudhana Arry Yanuar Asnawati Asnawati Azzahra, Maharanianska Berkahingrum, Ayu Bustamam, Alhadi Dalia Sukmawati Danang Nugroho, Danang Dendy K. Pramudito Eddyono, Fauziah El Enshasy, Hesham El-Enshasy, Hesham Ali Fahriza, Tiara Firdaus, Muhammad Fawaris Firman Maulana, Firman Harisman Harisman, Harisman Imam Riadi Jefferson Lynford Declan Juliansyah, Dandy Akbar Jumriati, Jumriati Kartika, Irma Ratna Kirwelakubun, Andreas Kmg, Arik Juliana Putra Krisdawati, Ismaya Kurniadewi, Fera Maghfira, Laila maisya, Bilqis Mohamad, Norfidah Muhammad Kunta Biddinika Muktiningsih Nurjayadi Mustapa, Muhammad A. Nagib, Muhammad Nay, Dian Nofitasari Umbu Noor Nailie Azzat Nurida, Nurida Nurlina, Ida Pradini, Purnama Sakhrial Prasetya, Deni Fami Putri, Adinda Myra Amalia Putri, Gladys Indira Putri, Gusti Angieta Rahmawati, Atikah Nur Ramdani, Aditiya Ramdani, Aditya rifa, Nisrina Rusni Rusni, Rusni Rusydi Umar S, Rosmarina Saamia, Vira Saamia4, Vira Sabilla, Alzena Dona sahara, Salsa Sambonu, Yurika Febiyani Sandra, Ni Putu Nita Ayu Saputro, Dwi Anna Oktaviani saputro, heru Septianingrum, Laely Wahyunita Setiawan, I Made Benny Shangkara, Muhammad Arkent Sikumbang, Kenanga M. Sri Haryuni SRI RAHAYU Suantari, Luh Ari Sulhianita, Sulhianita Suluh, Reyvaldo Febrian Rizki Sunardi Sunardi Sutriyo Sutriyo, Sutriyo Syamsudin, Fatih Syarif Aminul Khoiri Thomas, Nur A. Thoriq, Aqmal Titis Nurina Tumanggor, Rahmayanti Waluyo, Retno winarti, eko Wiranatha, I Made Wulandari, Oca Meilika Zakaria, Mohamad Rahmatullah Zein, Wahid Alfaridsi Achmad Zulfi Abdullah