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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Sainstek Journal of Marine Research JURNAL ILMU PERTANIAN Bioedukasi: Jurnal Pendidikan Biologi Biology, Medicine, & Natural Product Chemistry Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Ilmiah Peuradeun Jurnal Biodjati Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Formatif: Jurnal Ilmiah Pendidikan MIPA Assimilation: Indonesian Journal of Biology Education Jambura Edu Biosfer Journal Jambura Journal of Health Sciences and Research Jambura Journal of Chemistry BIOSFER : Jurnal Biologi dan Pendidikan Biologi Jurnal Review Pendidikan dan Pengajaran (JRPP) RADIAL: JuRnal PerADaban SaIns RekAyasan dan TeknoLogi JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Spizaetus: Jurnal Biologi dan Pendidikan Biologi Jurnal Pengabdian kepada Masyarakat Nusantara Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Jurnal Kewarganegaraan Nusantara: Jurnal Pendidikan Indonesia Journal of Research in Instructional BIOMA : Jurnal Biologi dan Pembelajarannya BIOVALENTIA: Biological Research Journal Makara Journal of Science JURNAL BIOLOGI PAPUA Journal of Tropical Mycorrhiza Jurnal Rekayasa elektrika Jurnal Biologi Al-Hayat: Natural Sciences, Health & Environment Journal Biodik: Jurnal Ilmiah Pendidikan Biologi HUMANIORASAINS Tumbuhan : Publikasi Ilmu Sosiologi Pertanian dan Ilmu Kehutanan Science: Indonesian Journal of Science Jurnal Cendekia Ilmiah Jurnal Pendidikan Kimia, Fisika dan Biologi RADIAL: Jurnal Peradaban Sains, Rekayasa dan Teknologi Inventa: Journal of Science, Technology, and Innovation Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Novri Youla Kandowangko
Universitas Negeri Gorontalo
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Induksi Kalus dari Antera Pisang Goroho (Musa acuminata L.) dengan Menggunakan 2,4-D dan BAP Risna A. Onu; Jusna Ahmad; Febriyanti, Febriyanti; Novri Youla Kandowangko; Devi Bunga Pagalla; Indriati Husain
Journal of Science, Technology, and Innovation Vol 1 No 2 (2025): December: Inventa: Journal of Science, Technology, and Innovation
Publisher : CV SCRIPTA INTELEKTUAL MANDIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.65310/8t6ph238

Abstract

Callus induction is a critical stage in anther culture for supporting the propagation and genetic improvement of local banana cultivars. This study evaluated the effect of different combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) on callus induction from goroho banana (Musa acuminata L.) anthers. Observations were conducted for 30 days after induction, focusing on callus emergence time and morphological characteristics, including color and texture. The results demonstrated distinct responses among treatments. The combination of 1.5 ml 2,4-D and 1.5 ml BAP produced the fastest callus initiation with an average of 13.17 days after induction and generated friable callus with a pale yellow coloration, indicating high cellular activity. Lower growth regulator concentrations delayed callus formation, while higher concentrations promoted color changes associated with early tissue differentiation. No callus formation was observed in the absence of exogenous growth regulators. These findings highlight the importance of balanced auxin and cytokinin concentrations in regulating anther-derived callus induction and provide a physiological basis for further development of goroho banana anther culture systems.
Empowerment of Mekar Jaya Farmer Group through Eco Farming and Digital Marketing for Optimization of Coconut and Corn Diversification Products: Pemberdayaan Kelompok Tani Mekar Jaya melalui Ecofarming dan Digital Marketing untuk Optimalisasi Produk Diversifikasi Kelapa dan Jagung Febriyanti Febriyanti; Novri Youla Kandowangko; Arif Dwinanto; Iswanto Bakari; Miftahul F. Adudu; Adila Nindhaulhasanah
JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Vol. 9 No. 1 (2025): Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Publisher : DPD Jatim Perkumpulan Dosen Indonesia Semesta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The Mekar Jaya Farmers Group has great potential in utilizing coconut and corn for diversification of value-added products. However, limited knowledge of environmentally friendly agricultural techniques (Ecofarming) and access to digital marketing are the main obstacles in optimizing coconut and corn diversification products. This community partnership program (PKM) aims to empower the Mekar Jaya Farmers Group through the application of ecofarming methods and the introduction of digital marketing strategies to increase productivity and the selling value of diversified products. Activities include education and training in making organic fertilizers, managing organic waste, diversification of coconut and corn waste products such as cocopeat, cocofiber, and corn-based snacks, as well as assistance in using digital platforms for marketing. The results of the activities showed an increase in the understanding and skills of the Mekar Jaya Farmers Group regarding ecofarming practices by 85%, diversification of coconut waste products (80%), diversification of corn (75%) and the reach of product marketing through digital media increased by 70%. This program not only provides new understanding to farmers about the importance of sustainable agricultural practices, but also opens up wider opportunities in marketing their products through digital technology. The implementation of ecofarming, which maintains environmental sustainability, and the use of digital marketing , coconut and corn-based diversified products now have higher competitiveness in the market and are able to support the optimization of agricultural products as well as environmental sustainability.
Induksi Kalus pada Tangkai Daun dan Batang Muda Tepurang (Momordica Cochinchinensis) dengan Kombinasi Bap dan Iaa Secara In Vitro Fadlia Dunggio; Devi Bunga Pagalla; Jusna Ahmad; Novri Youla Kandowangko; Febriyanti Febriyanti
Science: Indonesian Journal of Science Vol. 1 No. 6 (2025)
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/science.v1i6.246

Abstract

Tepurang (Momordica cochinchinensis) memiliki potensi besar dalam bidang farmasi dan pangan, namun keterbatasan bibit dan rendahnya tingkat perkecambahan biji menghambat perbanyakannya. Kultur jaringan menjadi alternatif dalam pelestarian dan perbanyakan tanaman ini, terutama melalui induksi kalus. Penelitian ini bertujuan untuk mengevaluasi pengaruh kombinasi Benzylaminopurine (BAP) dan Indole Acetic Acid (IAA) terhadap induksi kalus dari batang muda M. cochinchinensis secara in vitro. Eksplan berupa batang muda ditanam pada media Murashige & Skoog (MS) dengan kombinasi BAP dan IAA dalam berbagai konsentrasi. Hasil penelitian menunjukkan bahwa perlakuan dengan kombinasi ZPT mampu menginduksi pembentukan kalus dengan warna putih dan tekstur friable setelah 2 minggu subkultur. Temuan ini diharapkan dapat berkontribusi dalam pengembangan metode perbanyakan M. cochinchinensis secara in vitro untuk mendukung konservasi dan pemanfaatannya dalam bidang bioteknologi.
Actinomycetes from Plant Rhizosphere in Gorontalo Karst Area as Plant Growth Promoting Rhizobacteria Citra Leonita Matalauni; Yuliana Retnowati; Abubakar Sidik Katili; Novri Youla Kandowangko; Ani M. Hasan
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1047-1053

Abstract

This study aimed to describe Actinomycetes from the rhizosphere of plants in the Gorontalo karst area as Plant Growth Promoting Rhizobacteria (PGPR). The research method is based on a quantitative descriptive method. Soil samples from the rhizosphere were collected using a purposive sampling technique from 8 plant species at two locations in the Gorontalo karst region, namely the Tanjung Kramat Hills. The characteristics of PGPR in this study focused on phosphate solubilization ability, Indole Acetic Acid (IAA) production, antagonism against the fungus Fusarium oxysporum, and tolerance to fungicides. Potential PGPR isolates were analyzed for phylogenetic relationships based on 16S rRNA gene sequences. The results showed that there were 6 actinomycetes isolates (RzHKC-01, RzKO-02, RzO-03, RzAK-04, RzPK-05, and RzOC-06) from 8 plant species in the Gorontalo karst region. One actinomycetes isolate, RzPK-05, showed potential as a PGPR with the ability to solubilize phosphate and produce IAA at 0.165 mg/L and 0.133 mg/L, respectively. Isolate RzPK-05 exhibited antagonistic properties against Fusarium oxysporum with an inhibition rate of 82.24% and was tolerant to fungicides such as Captive, Benlate, and Thiramo up to a concentration of 100 mg/L. Molecular analysis based on the 16S rRNA gene and phylogenetic tree reconstruction indicated that the RzPK-05 isolate is closely related to the genus Streptomyces with 100% similarity.
Pengaruh Ekstrak Tauge terhadap Perkecambahan Benih Padi (Oryza Sativa L.) yang Lewat Masa Simpan Mega Rosalita Ekaputri Koni; Jusna Ahmad; Devi Bunga Pagalla; Novri Youla Kandowangko; Magfirahtul Jannah
Jurnal Pendidikan Kimia, Fisika dan Biologi Vol. 2 No. 1 (2026): Januari : Jurnal Pendidikan Kimia, Fisika dan Biologi
Publisher : Asosiasi Riset Ilmu Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/jupenkifb.v2i1.926

Abstract

The decline in seed quality due to storage beyond the shelf life is a major problem in rice cultivation. One effort that can be made to improve seed viability is through invigoration techniques using natural ingredients, such as bean sprout extract (Phaseolus radiatus), which contains growth hormones. This study aims to analyze the effect of bean sprout extract on the viability of Ciherang rice seeds that have exceeded their shelf life and to determine the best treatment. The study was conducted from August to November 2024 at the Biology Laboratory of the UPTD Seed Center, Supervision and Certification of Agricultural Seeds of Gorontalo Province. The study used a two-factor Randomized Block Design (RBD) with bean sprout age (3, 5, and 7 days after sowing) and bean sprout extract concentration (20 g/L, 40 g/L, and 60 g/L) as factors, with four replications. The parameters observed included germination rate, maximum growth potential, and sprout length. The data were analyzed using ANOVA and DMRT post-hoc test at a 5% level. The results showed that bean sprout extract had a significant effect on all observed parameters. The treatment of 5-day-old bean sprouts with a concentration of 20 g/L gave the best results with a germination rate of 95.5%, maximum growth potential of 98.5%, and the highest sprout length. Sprout extract has the potential to be used as a natural alternative to improve the quality of rice seeds that have passed their storage period.
Analisis Vegetasi di Kawasan Karst Provinsi Gorontalo Ali, Fathur Rahman M.; Katili, Abubakar Sidik; Husain, Ilyas H.; Utina, Ramli; Kandowangko, Novri Y.; Hamidun, Marini Susanti
J-CEKI : Jurnal Cendekia Ilmiah Vol. 4 No. 2: Februari 2025
Publisher : CV. ULIL ALBAB CORP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56799/jceki.v4i2.8292

Abstract

This study aims to determine the composition of vegetation types in the karst area of Gorontalo province and determine the vegetation structure in the karst area of Gorontalo province. The method used in this study uses the square transect method, the transect line is made perpendicular along 100 m, with the plot size being 20 x 20 m for tree strata, 10 x 10 m for pile strata, and 5 x 5 m for seedling strata, The results of vegetation structure analysis based on the highest INP value for tree-level strata at station I are Leucaena leucocephala species 2.20%, at Ceiba petandra station II 1.24%, and at Muntingia calabura station III 1.72%. At the stake level, the Leucaena leucocephala species had the highest INP value of the three stations, which was 1.81%. For the nursery rate, the highest INP is owned by the Leucaena leucocephala species at 2.10%. Based on the results of the analysis using ordinance charts, stations II and III have almost the same formation pattern so that the graph tends to overlap, while for station I it is very different from stations II and III, for station I it has its own formation pattern compared to stations II and III, and station I is also indicated to have its own characteristics based on habitat conditions and environmental factors.
Antagonistic Ability and Fungicide Tolerance of Rhizosphere Actinomycetes from the Hungayono Karst Ecosystem, Gorontalo Nazifah R Adam; Yuliana Retnowati; Abubakar Sidik Katili; Novri Youla Kandowangko; Wirnangsi D. Uno
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.895-905

Abstract

Actinomycetes show promise as biological control agents, reducing fungicide use in agriculture due to their bioactive compounds and ecological resilience. This study focused on the initial screening and ecological bioprospecting of actinomycetes from the Hungayono karst ecosystem in Gorontalo by determining their antagonistic activity against the pathogenic fungi Fusarium oxysporum and Neocosmospora solani, as well as their tolerance to the fungicides thiram and benomyl. Potential isolates were identified using molecular methods. Samples were collected from three locations. Rhizosphere soil samples were obtained from plant species: Alocasia macrorrhizos, Ficus microcarpa, and Acrostichum aureum L. Antagonistic activity against root-pathogenic fungi and tolerance to fungicides were used as initial screening criteria to assess their potential as biological control agents. This study successfully isolated 9 actinomycete isolates. The results showed that only 33% of the isolates exhibited inhibitory activity, with isolate RzAH-07 showing a moderate inhibition zone (5.22 mm) against Fusarium oxysporum and weak inhibition (4.18 mm) against Neocosmospora solani, while also demonstrating tolerance to the fungicide benomyl up to 1000 ppm. RzAH-07 was selected as the top candidate due to its two key advantages. Phylogenetic analysis based on 16S rRNA gene sequences indicates that RzAH-07 is closely related to the genus Streptomyces, with a similarity percentage ranging from 98.35% to 99.14% based on GenBank data.
Phosphate-Solubilizing and Indole Acetic Acid-Producing Actinomycetes from the Plant Rhizosphere in the Hungayono Karst, Gorontalo Yusriyah Yusriyah; Yuliana Retnowati; Abubakar Sidik Katili; Novri Youla Kandowangko; Wirnangsi D. Uno
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.907-917

Abstract

Actinomycetes are soil microorganisms that play a crucial role in nutrient cycles and interactions, particularly in nutrient-limited environments such as karst ecosystems. This study aimed to evaluate the preliminary functional potential of actinomycetes isolated from the plant rhizosphere in the Hungayono karst ecosystem, Gorontalo, as phosphate solubilizers and producers of indole acetic acid (IAA). The study employed a quantitative descriptive approach with soil sampling at a depth of 10–15 cm using purposive sampling. Isolation was performed using the spread plate method on Starch Casein Agar medium, followed by qualitative and quantitative testing of phosphate solubilization and IAA production capabilities. Identification of selected isolates was conducted through 16S rRNA gene-based phylogenetic analysis. The results showed that 11 actinomycete isolates were obtained from 4 host plants, of which two isolates exhibited low-index qualitative phosphate solubilization activity. However, quantitative results indicated that some isolates that did not form clear zones still produced measurable amounts of soluble phosphate, suggesting differences in method sensitivity. The highest phosphate-solubilizing activity was exhibited by isolate RzPO-09. In addition, four isolates were capable of producing IAA at varying levels, with the highest production observed in isolate RzPH-07 at 0.896 ppm. Phylogenetic analysis showed that isolate RzPO-09 (PZ458677) is closely related to the genus Pseudonocardia, while RzPH-07 (PZ458434) is closely related to the genus Streptomyces. Overall, the isolates obtained show initial functional potential; however, further validation is required through optimization of culture conditions, genetic characterization, plant growth tests, and testing both in greenhouses and in the field to ensure their practical feasibility.
Co-Authors Abubakar Sidik Katili Abubakar Sidik Katili Adila Nindhaulhasanah Adju, Fitria Y.Hs. Ahaya, Cindi Ahmad Hidayat Ahmad, Aisyah Akbar, Muh Nur Alfandi Kibu Ali, Fathur Rahman M. Andi Pratiwi Andi Pratiwi Ani Hasan, Ani Ani M. Hasan Aryati Abdul Aryati Abdul Bakari, Iswanto Bambang Mamangkay Bulowe, Yuniarty R Chairunnisa Lamangantjo Citra Leonita Matalauni Cono, Titi Hawanda Metania Dali, Ira K. Damopolii, Insar Devi Bunga Pagalla Devi Bunga Pagalla Dewi Wahyuni K. Baderan Djafar, Fitri Yani Djuna Lamondo Dwinanto, Arif Elawati, Elawati Elya Nusantari Fadlia Dunggio Fathur Rahman M. Ali Fatmariani Abdul Wahab Febriyanti febriyanti febriyanti Febriyanti Febriyanti Febriyanti Febriyanti Frida Maryati Yusuf GIAT SURYATMANA Gintulangi, Sabriana Oktaviana Gogou, Elsi Hamzah B Uno Husain, Holiyana Husain, Ilyas H. Ilyas Husain Indri Meliyani Bouta Indriati Husain Isima , Lindawaty Iswanto Bakari Jan Hendriek Nunaki Jusna Ahmad Kadita P. Latif Keley, Umar Keley, Umar Labuga, Falerins ladoma, wafiq aziza m. Lamatenggo, Nina Lindawaty Isima Magfirahtul Jannah Margaretha Solang Marini Susanti Hamidun Masra Latjompoh Mega Rosalita Ekaputri Koni Miftahul F. Adudu Miftahul Fikriyah Adudu Muchtar Ahmad Muh. Nur Akbar Mukhlisulfatih Latief Mustamin Ibrahim Mustaqimah, Nur Mutmainah Mutmainah Nazifah R Adam NENNY NURLAENY Nurdin Nuryanti Rumalolas Pagala, Devi Bunga Pakaya, Melisa Patta Sija Potutu, Bayu S. Putri, Tarissa Eka Rahman, Iyam Rahwilin Van Gobel Ramli Utina Rampi Yusuf Rianjani, Devi Tutut Rianjani, Devi Tutut Rinna Amelia Polihito Risna A. Onu Rizal Suleman Rizaldi Mokodompit ROBERT DJONGGI MARULI SIMANUNGKALIT Rosely, Meilin S. Y. Sahat Patar Lumban Gaol Saifullah Mamonto Suwarni Karim Syafar, Sitti Zaenab Tadidik, Ulfaida H. Ui, Maryam Walangadi, Farhana Rahmatia Wilinda A. Datau Wirnangsi Din Uno Wirnangsih D. Uno Yelsi Patiung Yuliana Nurdian Toini Yuliana Retnowati Yumna Binti Abud Alamri Yusrin Anton Puluhulawa Yusriyah Yusriyah Zuliyanto Zakaria Zusriadi, Brenda Febrina