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Helen, Helen
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Journal : Seminar Nasional Lahan Suboptimal

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Efektivitas Beberapa Metode Sterilisasi terhadap Tingkat Kontaminasi Eksplan Tulang Daun Duku pada Kultur In-Vitro Inaya, Rihani; Irmawati, Irmawati; Susilawati, Susilawati; Helen, Helen; Herlin, Weri; Negara, Zaidan P.
Seminar Nasional Lahan Suboptimal Vol 12, No 1 (2024): Vol 12, No 1 (2024): Prosiding Seminar Nasional Lahan Suboptimal ke-12 “Revital
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Inaya, R., Irmawati, I., Susilawati, S., Helen, H., Herlin, W., & Negara, Z. P. (2024). The effect of different sterilization techniques on the contamination levels of duku leaf midrib explant in In-Vitro Culture. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-12 Tahun 2024, Palembang 21 Oktober 2024. (pp. 667–678). Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).The Duku plant (Lansium domesticum) is one of the popular tropical fruit in indonesia. Using the idea of plant cells' totipotency, tissue culture is an alternate method for a quick and massive propagation of high-quality duku plant seedlings. This research was aimed to evaluate different sterilization materials and procedures on duku leaf midrib explants through tissue culture. The research was conducted from June to September 2024 at Tissue Culture Laboratory, Department  of  Agronomy, Universitas Sriwijaya (2°59’23.4”S 104°43’53.4”E). A combination of  sterilizing substances, including 70% alcohol, fungicide, bactericide, liquid detergent, and NaOCl, were utilized in this sterilization procedures. The percentage of live explants, browning explants, and explants infected with bacteria or fungi then were calculated using parametric descriptive analysis. The results showed that the P3 treatment's sterilant combination liquid detergent for 3 minutes, 0.2% of fungicide and bactericide for 10 minutes, 1% NaOCl for 15 minutes, and 70% alcohol for 5 minute produced the highest percentage of live explants (46%) with a relatively low percentage of contaminated explants (30%) and the lowest percentage of browning explants (23%). Thus P3 treatment was concluded as the best treatment to reduce the contamination level and browning of duku leaf midrib explant.
Co-Authors Achir Yani S. Hamid Afriyadi, Afriyadi Agoes Dariyo Alexios, Constantine Alfyoni, Ghisanie Azahra Amesz, Mutiara Fransisca Anandita, Rafela Angel Angel Apriani, Elin Ardela, Yara Athya, Safana Zakira Chen, Chin Fong Chendana, Lewis Edbert Christianto, Anastasia Bernessa Christiarini, Renny Dahlia, Debie - Daulay, Muhammad Anggie Januarsyah Erryson, Davin Evi Martha Fahlevi, Renza Firman Pangaribuan Gabriela, Angelin Gaol, Mutiara Sani Lumban Gunadi, Alvin Nicolas HASIM PURBA Herman, Sylvia Hutasoit, Ramalia Inaya, Rihani Irmawati Irmawati Jess, Sharon Khairunnisa, Julia Kudri, Wan Muhamad Kurniati, Echi Kurniawan, Roy Laudyto, Anugerah Prasetyo Lunda, Axel Nathanael Manalu, Yohana Gusvita Marliani, Sari Michelle, Laurencia Muhammad Ridwan Mulatsih, Endang Sri Negara, Zaidan P. Ng, Octarianto Lika Patriot, Evelina Astra Putri, Aneesa Joenice Putri, Regita Elsa Raiis, Daffa Dzaki Ramadani, Naila Ratna Aryani Riwanda, Josephine Kayla Rosnidar Sembiring Rusliwati, Ermina Saraswati, Kiky Dwi Hapsari Sardianto Markos Siahaan, Sardianto Markos Sartika, Cut Hayyu Satria, Muhammad Ryo Dwi Scristia, Scristia Sebastian, Bobby Shereen, Shereen Silalahi, Aulia Sonda Sinambela, Fitriana Aidnilla Susilawati, Susilawati Suyono Suyono Syarif, Iif Ahmad Tambunan, Lois Tindaon, Elisabeth Indira Dameria Toar JM Lalisang Umi Kalsum Vincent Vincent, Vincent Viny Christanti M Vivikoh, Vivikoh Wandestarido, Wandestarido Weri Herlin Wicaturatmashudi, Sukma Wijaya, Selvi Elyana Winsen, Winsen Wulan Purnama Sari Yanti, Bely Novi Yasser, Achmad Yuliawati, July Yunitasari, Masrina