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Journal : International Journal of Health and Pharmaceutical (IJHP)

Antioxidant Activity Test Of Methanol Extract Of Gaharu (Aquilaria Malaccensis Lam.) Bark With Dpph (1,1 Diphenyl-2-Picrylhydrazyl) Method Ridwanto Ridwanto; Asep Trizaldi; Zulmai Rani; Anny Sartika Daulay; Haris Munandar Nasution; Dikki Miswanda
International Journal of Health and Pharmaceutical (IJHP) Vol. 3 No. 2 (2023): May 2023
Publisher : CV. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51601/ijhp.v3i2.123

Abstract

Gaharu (Aquilaria malaccensis Lam.) is one of the natural ingredients that can be used as an antioxidant. Antioxidants are compounds that can inhibit free radical reactions in the body. Free radicals have an impact on the pathogenesis of several diseases in humans due to oxidative stress in cells. The purpose of this study was to determine the class of secondary metabolites of agarwood bark and antioxidant activity and to compare the value of IC50 (Inhibitory concentrations) of the methanol extract of agarwood bark with the IC50 of vitamin C in the DPPH method using UV-Vis spectrophotometry. The methanol extract of agarwood bark was macerated using methanol, then determined the content of secondary metabolites of Simplicia. The determination of antioxidant activity was carried out on the methanol extract of agarwood bark with the addition of DPPH at various concentrations. The sample concentrations were 20, 40, 60, 80, and 100 µg/mL.. As a comparison, vitamin C was used with concentrations of 4, 8, 12, 16 and 20 µg/mL.. Then they calculated percent attenuation (% inhibition) and the value of IC50. The results of the screening on the methanol extract of agarwood bark contained chemical compounds such as flavonoids, saponins, and tannins. The determination of antioxidant activity was carried out using a UV-Vis spectrophotometer with the DDPH method. The results were obtained from the methanol extract of the bark of agarwood, which has antioxidant activity in the strong category with an IC50 value of 94.59 µg/mL.and vitamin C in the very strong category with an IC50 value of 22,11 µg/mL.
Co-Authors AA Sudharmawan, AA Agus Junaidi Alexander Sebayang Ani Sutiani Anny Sartika Daulay Asep Trizaldi Aulia Fitri, Aulia Aulia Sari Awanis, Safirah Bambang Hermanto Bambang Hermanto Cut Dian Mala Luthfia D. Elysa Putri Mambang Devi Lestari Dina Suciati Saragih Dinda Sari Utami Efendi, Tania Tiara Faradilla, Putri Fatma Dwi Jati Fithri Pulungan, Ainil Fitri, Raissa Gabena Indrayani Dalimunthe Haris Munandar Nasution Imas Masriah Jasmidi Khairahmi, Khairahmi Khofifah , Nur Kiki Rawitri Lubis, Minda Sari Lubis, Seri Lestari Moondra Zubir Moondra Zubir Muflikhah Muflikhah Muflikhah, Muflikhah Nasution, M Pandapotan Nasution, Paujia Nasution, Shamila Sufi Aulia Natasya, Siti Adinda Ningtias, Anggitha Nomi Noviani Novrizaldi Wardana Nur Mutia Rosiati Nurmala, Syandoval Triska Ananda Nuryono Nuryono Pulungan, Ahmad Shafwan Puteri, Cut Intan Annisa Rafika, Intan Maya Rafita Yuniarti Rahayu , Yayuk Putri Rahmadi Ali Ramadhan, Dwi Sapri Ramadhan, Zulkifli Reza Irma Ricky Andi Syahputra Ridwanto Ridwanto Ridwanto Rini Selly Rini Selly, Rini Riski Mulyani, Riski Rosiati, Nur Mutia Safira , Liyuza Saputra, Muhammad Fadhlan Sari, Syilvi Rinda Sari, Tiara Puspita Savika, Anggraini Selly, r Sembiring, Rinawati Siregar, Mutia Nabila Siti Rahmah Siti Rahmah Sitompul, Maysaro SP, Ananda Azzahra Sri Adelila Sari Sri Wahyuni Sulwiyatul K Sani Supiyani, Supiyani Surya, Innaya Tantry Syafirna, Nada Syilvi Rinda Sari Tuzzahra, Safira Yayuk Putri Rahayu Yuniarti, Rafita Zubir, Moondra Zulmai Rani