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Uji Aktivitas Soyghurt Ekstrak Daun Pucuk Merah (Syzygium myrtifolium [Roxb] Walp.) dan Soyghurt Ekstrak Bunga Telang (Clitoria ternatea L.) Terhadap Bakteri Escherichia coli Susanti, Susanti; Afriliani, Sindy; Mardianingrum, Richa
Jurnal Farmasi & Sains Indonesia Vol 6 No 2 (2023)
Publisher : LPPM Sekolah Tinggi Ilmu Farmasi Nusaputera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52216/jfsi.vol6no2p102-111

Abstract

Diarrhea is a disease characterized by changes in the form of feces and an increase in the frequency of bowel movements that are more frequent than usual. One of the causes of diarrhea is infection by Escherichia coli which is a normal microflora in the human large intestine that helps the process of food spoilage, but can be pathogenic if the number increases. Red shoot leaves (Syzygium myrtifolium [Roxb] Walp.) and butterfly pea (Clitoria ternatea L.) contain anthocyanins, which are compounds derived from flavonoids which have antibacterial properties. The purpose of this study was to determine the presence of antibacterial activity in soyghurt preparations with a combination of red shoots and soyghurt leaf extract with a combination of butterfly pea flowers, and to determine the most effective concentration for inhibiting the growth of Escherichia coli bacteria using the well-diffusion method. The results showed that soyghurt red shoots leaf extract had antibacterial activity against Escherichia coli bacteria with an inhibition zone diameter of 4.60 mm at a concentration of 10% and 7.95 mm at a concentration of 20%. Whereas in soyghurt butterfly pea flower has antibacterial activity against Escherichia coli bacteria with an inhibition zone diameter of 35.35 mm at a concentration of 5%, 38.68 mm at a concentration of 10% and 40.18 mm at a concentration of 20%.
PEMBERDAYAAN KELOMPOK WANITA TANI DALAM MENGOLAH SAYURAN ‘BON-YUR’ SEBAGAI MAKANAN SEHAT TAMBAHAN Sonya Nurizki Vikandari; Richa Mardianingrum; Adinda Putri Amanda; Mochamad Herdi Nurzaman
JMM (Jurnal Masyarakat Mandiri) Vol 8, No 6 (2024): Desember
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v8i6.27023

Abstract

Abstrak: Kelompok Wanita Tani (KWT) Mawar Bodas merupakan kelompok pemberdayaan wanita bidang pertanian yang memiliki permasalahan minimnya pengetahuan terkait pengolahan sayuran hasil panen yang tidak laku dijual. Tujuan pengabdian masyarakat ini yaitu meningkatkan pengetahuan dan keterampilan KWT dalam mengolah sayuran menjadi makanan sehat tambahan Bon-Yur (abon sayur) sebagai inovasi baru dalam bentuk dan rasa yang berbeda. Metode yang digunakan dalam pengabdian ini yaitu Forum Group Discussion, penyuluhan, pelatihan, monitoring dan evaluasi (pre-test dan post-test). Mitra dalam pengabdian ini yaitu KWT Mawar Bodas yang beranggotakan 25 orang. Hasil kegiatan pengabdian ini terdapat peningkatan pengetahuan mengenai materi pengolahan sayuran dan pentingnya sayuran bagi tubuh sebesar 92,6%, sebesar 100% peserta antusias untuk mengolah sayuran menjadi bon-yur serta sebesar 80% peserta tertarik untuk memproduksi bon-yur. Hal ini menunjukkan bahwa KWT memiliki potensi kreativitas dalam memajukan kesejahteraan ekonomi serta pentingnya pengabdian lanjutan sebagai tindak lanjut pembuatan bon-yur.Abstract: The Mawar Bodas Women Farmers Group (KWT) is a women’s empowerment group in the agricultural sector that has a problem of lack knowledge related to processing harvested vegetables that are not sold. The aim of this community service is to increase the knowledge and skills of KWT in processing vegetables into additional healthy food Bon-Yur (vegetable floss) as a new innovation in different forms and flavors. The methods used in this service are forum group discussion, counseling, training, monitoring, and evaluation (pre-test and post-test). The partner in this service is KWT Mawar Bodas Tasikmalaya, which has 25 members. The result of this service activity shows an increase in knowledge about vegetable processing and importance of vegetables for the body by 92.6%, with 100% of participants were enthusiastic about processing vegetables into bon-yur and 80% of participants were interested in producing bon-yur. This shows that KWT has the potential for creativity in advancing economic welfare and the importance of further service as a follow-up to making bon-yur.
In Silico Study of Corn Silk Luteolin (Zea mays L.) Derivatives as Potential Antihypertensive Agents Mardianingrum, Richa; Firiani, Yuli; Endah, Srie Rezeki Nur; Ruswanto, Ruswanto
Jurnal Kimia Sains dan Aplikasi Vol 28, No 3 (2025): Volume 28 Issue 3 Year 2025
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.28.3.155-167

Abstract

Hypertension is a condition where the systolic blood pressure is > 140 mmHg and the diastolic pressure is > 90 mmHg. Hypertension is caused by the formation of Angiotensin II from Angiotensin I by Angiotensin Converting Enzyme (ACE). Lisinopril is one of the drugs commonly used to treat hypertension; however, long-term use may be associated with carcinogenic effects. This study aims to find candidates for new medicinal ingredients from luteolin derivative compounds contained in corn silk (Zea mays L.) as antihypertensives that have the activity of inhibiting ACE enzymes. Molecular docking and molecular dynamics simulations were employed in this study. The results showed that the TL59 compound exhibited lower predicted toxicity than lisinopril. Based on molecular dynamics analysis, TL59 demonstrated an RMSD value of 1 Å and a ΔGTOTAL of –44.65 kcal/mol, whereas lisinopril showed an RMSD value of 1.3 Å and a ΔGTOTAL of –29.25 kcal/mol. These findings suggest TL59 has a higher binding affinity and greater stability toward the 1O86 receptor than lisinopril. Therefore, TL59 is predicted to be a promising candidate for a new antihypertensive drug that inhibits the conversion of Angiotensin I to Angiotensin II. In conclusion, TL59 demonstrates strong binding affinity and pharmacokinetic properties, indicating its potential as a promising antihypertensive candidate. However, this study is limited to in silico analysis and requires further in vitro and in vivo validation to confirm its efficacy and safety.
Effect of Soyghurt Fortification with Syzygium myrtifolium Walp. Leaves Extract on Antioxidant Activity Susanti, Susanti; Rizkuloh, Lina Rahmawati; Mardianingrum, Richa
Journal of Food and Pharmaceutical Sciences Vol 13, No 2 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.21223

Abstract

Soyghurt contains antioxidant compounds such as isoflavones which is derived from soybeans as main ingredient for making soyghurt. To increase antioxidant activity, it is necessary to add other antioxidant compounds such as anthocyanins which is found in Syzygium myrtifolium Walp. which also acts as a red pigment. The aim of this study was to determine the effect of adding Syzygium myrtifolium leaf extract to the antioxidant activity of soyghurt. It began with the making of soy milk then fermented with Lactobacillus bulgaricus into soyghurt. Plants were extracted by maceration in ethanol and made a dry extract. The addition of dried extract to soyghurt was carried out in 3 concentrations, 1%, 3% and 5%. The results of antioxidant activity assay using the free radical DPPH scavenging method showed that there was a decrease in the IC50 in soyghurt fortified with extract compared to soyghurt without extract. The higher concentration of extract, the smaller IC50 value. Soyghurt with 5% extract had the smallest IC50 (694.14 g/mL) while the IC50 of soyghurt without extract was 2823.17 g/mL. The conclusion is that fortification of Syzygium myrtifolium leaf extract has an effect on the antioxidant activity of soyghurt.
Effect of Soyghurt Fortification with Syzygium myrtifolium Walp. Leaves Extract on Antioxidant Activity Susanti, Susanti; Rizkuloh, Lina Rahmawati; Mardianingrum, Richa
Journal of Food and Pharmaceutical Sciences Vol 13, No 2 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.21223

Abstract

Soyghurt contains antioxidant compounds such as isoflavones which is derived from soybeans as main ingredient for making soyghurt. To increase antioxidant activity, it is necessary to add other antioxidant compounds such as anthocyanins which is found in Syzygium myrtifolium Walp. which also acts as a red pigment. The aim of this study was to determine the effect of adding Syzygium myrtifolium leaf extract to the antioxidant activity of soyghurt. It began with the making of soy milk then fermented with Lactobacillus bulgaricus into soyghurt. Plants were extracted by maceration in ethanol and made a dry extract. The addition of dried extract to soyghurt was carried out in 3 concentrations, 1%, 3% and 5%. The results of antioxidant activity assay using the free radical DPPH scavenging method showed that there was a decrease in the IC50 in soyghurt fortified with extract compared to soyghurt without extract. The higher concentration of extract, the smaller IC50 value. Soyghurt with 5% extract had the smallest IC50 (694.14 g/mL) while the IC50 of soyghurt without extract was 2823.17 g/mL. The conclusion is that fortification of Syzygium myrtifolium leaf extract has an effect on the antioxidant activity of soyghurt.
In Silico Study of Green Tea (Camellia sinensis) Compound as Potential Anxiolytic Drug Material Targeting Estrogen Alpha Receptor Harfiah Nur Aini; Susanti; Richa Mardianingrum
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 12 No. 1 (2025): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v12i12025.93-103

Abstract

Background: The prevalence of anxiety disorders has significantly increased each year but has not been matched by the availability of adequate treatments. Estrogen receptor alpha (ERα) is known to induce anxiety through the activation of a complex system in the body, and drugs that inhibit ERα activity are predicted to have anxiolytic potential. Objective: This study aims to evaluate 50 compounds derived from green tea leaves to discover potential anxiolytic candidates that act by inhibiting ERα. Methods: The research methods used include toxicity screening, pharmacokinetic screening, drug scan, molecular docking, and molecular dynamics. Results: Based on the screening results, quercetin was identified as non-carcinogenic, non-hepatotoxic, easily absorbed, evenly distributed, non-interfering with CYP2D6 enzyme metabolism, and potentially effective as an oral drug. In molecular docking results, quercetin showed a ΔG value of -7.54 kcal/mol and Ki of 2.97 µM, which are better than the reference drug with a ΔG value of -7.20 kcal/mol and Ki of 5.24 µM. Quercetin also shown more stable interactions with the Erα binding site, indicated by amino acids Glu353 and Arg394 in RMSD and RMSF analysis during molecular dynamics simulation. Conclusion: From the study result it can be concluded that quercetin has potential as a good candidate for anxiolytic drug material by inhibiting ERα activity.
RENDEMEN DAN SKRINING FITOKIMIA EKSTRAK KULIT MANGGIS MENGGUNAKAN METODE EKSTRAKSI DAN PELARUT BERBEDA SEBAGAI ALTERNATIF SUPLEMEN PAKAN UNGGAS Walid, Rifan Afrian Nur; Kusmayadi, Andri; Frasiska, Nurul; Mardianingrum, Richa
Agrinimal Jurnal Ilmu Ternak dan Tanaman Vol 13 No 1 (2025): Agrinimal Jurnal Ilmu Ternak dan Tanaman
Publisher : Jurusan Peternakan Fakultas Pertanian Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajitt.2025.13.1.50-56

Abstract

Kulit buah manggis (Garcinia mangostana L.) telah diketahui mengandung banyak senyawa yang memiliki aktivitas antioksidan dan antibakteri. Kulit buah manggis dalam proses ekstraksi dilakukan dengan metode ekstraksi dan pelarut yang beragam. Penelitian ini bertujuan untuk mengetahui pengaruh metode ekstraksi menggunakan pelarut berbeda terhadap rendemen dan skrining fitokimia ekstrak kulit buah manggis yang ditunjukan sebagai suplemen pakan ternak unggas. Metode penelitian ini yaitu eksperimental dengan Rancangan Acak Lengkap (RAL) pola faktorial 3x2. Faktor pertama adalah pelarut (faktor A) A1 = etanol 96%, A2 = etil asetat, A3 = n-heksan. Faktor kedua adalah metode (faktor B) B1 = metode maserasi dan B2 = metode sokletasi yang menghasilkan 6 perlakuan. Parameter yang diamati adalah persentase rendemen dan skrining fitokimia ekstrak kulit manggis. Hasil dari penelitian ini yaitu persentase rendemen menunjukkan hasil yang berbeda sangat nyata (P<0,01). Adapun pada pengujian skrining fitokimia menunjukkan adanya kandungan tannin, polifenol, dan triterpenoid. Kesimpulan dari penelitian ini yaitu metode maserasi dengan pelarut etanol 96% menunjukkan persentase rendemen yang terbaik. Hasil dari penelitian ini dapat bermanfaat sebagai antioksidan alami bagi ternak khususnya unggas. ABSTRACT Mangosteen peel (Garcinia mangostana L.) has been known to contain many compounds that have antioxidant and antibacterial activity. Mangosteen peel in the extraction process is carried out with various extraction methods and solvents. This study aims to determine the effect of extraction methods using different solvents on the yield and phytochemical screening of mangosteen peel extract which is indicated as a poultry feed supplement. This research method is experimental with a Completely Randomized Design (CRD) factorial pattern 3x2. The first factor is the solvent (factor A) A1 = 96% ethanol, A2 = ethyl acetate, A3 = n-hexane. The second factor is the method (factor B) B1 = maceration method and B2 = soxhletation method which produces 6 treatments. The parameters observed are the percentage of yield and phytochemical screening of mangosteen peel extract. The results of this study, namely the percentage of yield showed very significant results (P<0.01). The phytochemical screening test showed the presence of tannin, polyphenols, and triterpenoids. The conclusion of this study is that the maceration method with 96% ethanol solvent shows the best yield percentage. The results of this study can be useful as a natural antioxidant for livestock, especially poultry.
Formulation and in-vitro antioxidant evaluation of α-mangostin, piperin, curcumin in oral patch and their combination Suharyani, Ine Suharyani; Mardianingrum, Richa; Falya, Yuniarti; Pratama, Rizki; Mas'ud, Ibnu; Daruwati, Isti
Pharmaciana Vol. 15 No. 2 (2025): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v15i2.29906

Abstract

Natural antioxidants have been developed in various formulations as alternatives to reduce the side effects associated with synthetic antioxidants. α-Mangostin exhibits strong antioxidant properties, although its behavior in combination with other herbal compounds remains unclear. The aim of this study was to formulate a combination of α-M, piperine, and curcumin into an oral patch and to evaluate the antioxidant properties of α-M when combined with piperine and curcumin, both known natural antioxidants. The oral patches were prepared by incorporating the active compounds (α-mangostin, piperine, and curcumin) into a matrix of hydroxypropyl methylcellulose (HPMC), gelatin, and chitosan. Glycerin was added as a plasticizer. Organoleptic assessment, along with evaluations of pH, weight, folding endurance, and moisture absorption capacity, confirmed the successful formulation of α-mangostin (MP), piperine (PP), curcumin (CP), and combination (MPCP) patches as oral delivery systems. All patches maintained a pH above 5.5. The thicknesses of MP, PP, CP, and MPCP were measured at 0.14 ± 0.02 mm, 0.17 ± 0.01 mm, 0.15 ± 0.02 mm, 0.11 ± 0.01 mm, and 0.16 ± 0.02 mm, respectively. The combination of these natural compounds enhanced folding endurance compared to the individual curcumin and piperine patches, while slightly reducing antioxidant activity. The co-formulation of α-mangostin, piperine, and curcumin into a single oral patch demonstrated a modest decline in antioxidant capacity. This study aimed to assess the impact of combining multiple antioxidant-rich natural compounds within a single delivery system.
Detection of Escherichia coli Contamination Using Most Probable Number (MPN) Methods of Jamu Pahitan in Singaparna District, Tasikmalaya Rahmawati, Syifa Sri; Mardianingrum, Richa; Susanti, Susanti
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.757-761

Abstract

Indonesians consume a lot of traditional jamu, including jamu pahitan. However, the hygiene aspect in the production and serving process is often neglected, so it has the potential to be contaminated with Escherichia coli. Using the MPN method, this study analyzed the presence of E. coli in jamu pahitan sold in Singaparna District. A total of 10 samples were tested, taken from jamu vendors who used plastic bottle packaging. The MPN test results showed that all samples contained E. coli with MPN values ranging from 6.0-1100 g/mL. None of the samples met food safety standards based on SNI 7388:2009, because the maximum limit of E. coli in drinks is <3 MPN/mL. The results of Gram staining of bacteria showed that the bacteria found were bacilli, red in color, including Gram-negative bacteria, indicating the presence of E. coli bacteria.
Papain-like Protease Peptides as Construction Material for the SARS-CoV-2 Vaccine Design Candidate: In-silico Study Mardianingrum, Richa; Pertiwi, Nur Ihsani; Rizkuloh, Lina Rahmawati; Ikram, Nur Kusaira Khairul; Ruswanto, Ruswanto
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 11, No. 1, May 2025
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.42748

Abstract

COVID-19 remains a major global health threat. In addition to implementing health protocols and consuming supplements, proactive prevention strategies are essential to limit the spread of the virus. One of the most promising approaches is the use of vaccines, particularly peptide-based vaccines, which are under active development. This study aimed to design a peptide vaccine derived from the SARS-CoV-2 papain-like protease (PLpro) and evaluate its interaction with key components of the human immune system, namely Toll-like receptor 3 (TLR3), major histocompatibility complex class I (MHC-I), and class II (MHC-II). The research employed an immunoinformatics approach utilizing NetCTL, IEDB Tepitool, PEP-FOLD3, trRosetta, HDOCK, GalaxyRefine2, and other molecular modeling tools. The designed vaccine construct was visualized in 3D using trRosetta and validated through ERRAT2, achieving a 100% quality score, indicating excellent structural integrity. The docking simulations demonstrated stable interactions between the vaccine and the immune receptors, suggesting strong immunogenic potential. In conclusion, the in silico-designed peptide vaccine based on SARS-CoV-2 PLpro shows promise in triggering immune responses through stable binding with TLR3, MHC-I, and MHC-II, highlighting its potential as a candidate for further experimental validation in COVID-19 vaccine development.
Co-Authors Aas Nuraisah Aas Nuraisah Aas Nuraisah Adinda Putri Amanda Afriliani, Sindy Aguslina Kirtishanti Agustien, Gina Septiani Ai Teni Siti Robi`ah Amelia, Widya Puspitasari Andri Kusmayadi Arif Budiman Arry Yanuar AYU RAHMAWATI Bachtiar, Kamiel Roesman Delis Susilawati Diah Lia Aulifa Dudi Nurmalik Elis Nurul Ikhlas Elsi Eryanti Fajar Setiawan Falya, Yuniarti Feri Sandria Firiani, Yuli Gatut Ari Wardani Gina Septiani Agustien Gina Septiani Agustien Gina Septiani Agustien Ginna Sri Nuryani Gramita, Widya Siva Hadiwijaya, Nathannael Adrya Harfiah Nur Aini Hartono, Sugianto Ayudha Ikram, Nur Kusaira Khairul Imam Mustaqim Garna Indah Cantika Indri Rahmawati Irma Andriani Isti Daruwati Jhoni, I Made Kamiel Roesman Bachtiar Korry Novitriani, Korry Kuncoro, Aditiya Kurniawan, Rian Liawardi, Petra Pahlawanda Chrisanto Lilis Tuslinah Lilis Tuslinah Lilis Tuslinah, Lilis Lina Rahmawati Rizkuloh Makiyatuzahro, Dede Avissa Mardhiah Mardhiah Mardhiah Mardhiah Mas'ud, Ibnu Meylany Sity Rossy Lestary Mia Nurfazriah Mida Hamidah Mochamad Herdi Nurzaman Monikasari, Hesti Muchtaridi Muchtaridi Nahariyah, St. Rohmani Napitupulu, Gloria Neta Ekayanti Suganda Nisa Uswatun Khasanah Nitya Nurul Fadilah Nofianti, Tita Nofriyaldi, Ali Nur Aji Nur Aji Nur Ihsani Pertiwi Nur Laeli Dwi Hidayati Nur Laili Dwi Hidayati Nur Rahayuningsih Nur Rahayuningsih, Nur Nurlaila, Putri Saniah Nurmalik, Dudi Nurmalik, Dudi Nursuhud Nursuhud Nursuhud Nurul Frasiska Oktariani, Anisa Pertiwi, Nur Ihsani Pratama, Rizki Pratita, Anindita Tri Kusuma Pratomo, Muhammad Fadhil Rahmawati, Syifa Sri Renadi, Sedin Rizgy Anggia Ruswanto, Ruswanto Ryan Apriandi Sa'banah, Nonah Sarwatiningsih, Yunia Sarwatiningsih, Yunia Septian, Ade Dwi Setyawati, Luthfi Utami Sindy Afriliani Siswandono, Siswandono Srie Rezeki Nur Endah Srie Rezeki Nur Endah Suhardiana, Eddy Suharyani, Ine Suharyani SUSANTI Susanti Susanti Susanti Susanti Susanti Susanti Syifa Alifia Lukman Tammy Riyanda Julianti Tati Herlina Tati Herlina Tifa Nofianti Tita Nofianti Tita Nofianti Tita Nofianti, Tita Tresna Lestari, Tresna Tuslinah, Lilis Unang Supratman Vikandari, Sonya Nurizki Walid, Rifan Afrian Nur Winda Trisna Wulandari Winda Trisna Wulandari Wirantono, Sebastian Nathanoel Yuliawati, Sri Yunia Sarwatiningsih