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All Journal Journal Of Vocational Health Studies Klinikal Sains (Jurnal Analis Kesehatan) Jurnal Riset Kesehatan Poltekkes Depkes Bandung INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Ners Jurnal teknologi Kesehatan Borneo MAHESA : Malahayati Health Student Journal Jurnal EduHealth JURNAL ILMIAH GLOBAL EDUCATION Innovative: Journal Of Social Science Research Jurnal Vokasi Kesehatan Jurnal Laboratorium Khatulistiwa Indonesian Journal of Community Empowerment Media Penelitian dan Pengembangan Kesehatan MEDICA (International Medical Scientific Journal) Jurnal Info Kesehatan ELECTRON (Journal of Science and Technology)
Ari Nuswantoro
Departement Of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak
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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

 1 
Potential of Garlic Filtrate as An Alternative Anticoagulant for Whole Blood Samples Ari Nuswantoro; Jessica Ningtyas Berlianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.2683

Abstract

Synthetic anticoagulants such as heparin, citric, and ethylenediaminetetraacetic acid (EDTA) are commonly used to prevent blood clots. In contrast, its widespread use in clinical laboratories is still constrained by price, its toxic nature, and its short shelf life. Therefore, an alternative material that is relatively cheap, non-toxic, and easy to obtain and process in a ready-to-use form is needed. Garlic contains allicin and ajoene, which are anti-platelet and anti-thrombogenic. This study’s aim is to explore the potential of garlic filtrate as an alternative anticoagulant.  Blood from 16 individuals was used and separated into four groups: non-anticoagulant, 50 μL/mL garlic filtrate, 100 μL/mL garlic filtrate, and 150 μL/mL heparin, for a total of 64 treatments. The Lee and White method showed that non-anticoagulated blood had normal clotting times (mean 8 minutes and 56 seconds), whereas heparin plasma and garlic filtrate plasma had longer clotting times (more than 20 minutes); and this is statistically different based on the ANOVA test with a significance value (p) of 0.000 < 0.05. On spectrophotometric measurements, the levels of calcium ions in heparinized plasma and serum were 8.66 mg/dL and 8.52 mg/dL, respectively, while in garlic plasma filtrate of 50 μL/mL and 100 μL/mL were 4.13 mg/dL and 3.58 mg/dL, respectively; this is also statistically different based on the ANOVA test with a significance value of 0.000 < 0.05. The differences indicate that garlic filtrate can extend clotting time and reduce calcium ions therefore it is worth reviewing as an alternative anticoagulant.
Co-Authors Adawiyah, Rabi’atul Aditia Aditia Aditia, Aditia Aditiya Ramadan Affifatuzahara Affifatuzahara Anisya Nur Shafitri Ardhani, Adha Riesta Arifah, Rizki Zanuba Astuti, Fitri Mega Aufani, Destia Aulia, Annisa Syafa Azura azura Azzahra, Larasabella Bagus Muhammad Ihsan Benedikta Anjelica Desi Wahyumarniasari Devi Nurfitri Bintang Dinasti Aprilia Dinasti Aprillia Djohan, Herlinda Edi Santoso Emilda Sari Fadilla, Alda Nur Fahma Ajeng Al-qorifah Fahrani, Chantika Lailatussa'adah Fitra Fathuhudin Grovindo Grovindo Hartati, Fitri Ulya Herlinda Djohan Imma Fatayati Indah Purwaningsih Indah Purwaningsih Indrawati, Ratih Jenny Ratnawati, Gervacia Jessica Ningtyas Berlianti Jordan, Aristo Juliana Cristyaningsih Kartini Kartini Laila Kamilla Laila Kamilla Linda Triana Linda Triana Linda Triana Liza Harum Nur Annisa Maretalinia Maretalinia Maretalinia, Maretalinia Maulidiyah Salim Merda Sri Rahayu Monalita, Ria Muhammad Ihsan, Bagus Munardi, Fhatia Natasya Natasya Intan Ramadhani Nurhayati, Etiek Nurul Uq'ma P Purwanto Panyada Cholsakhon Perdede, Lusi Hernawati Rahayu, Merda Sri Ramadan, Aditiya Ratnawaty, Gervacia Jenny Reza Setiawan Reza, Nur Rezky, Muhammad Andyka Riska Alfianita Rizki Zanuba Arifah Romadi Romadi Sadid, Randy Samwidya, Veronika Sriayu Sharen Sen Slamet Sri Tumpuk Sugito Sugito Sugito Sugito Supriyanto Supriyanto Supriyanto Supriyanto Sutriswanto Sutriswanto Suwandi, Edy Syahdilla, Ade Syopingi Syopingi Syopingi, Syopingi Tantri Rahmatika Tarisya Fitri Pratiwi Theresia Indah Budhy Sulisetyawati Tika, Dian Tumpuk, Sri Veny Veny Wahdaniah Wahyudi Wicaksono, Didik Yunus, Mohd