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Journal : Tropical Animal Science Journal

The Effect of Nanoencapsulated Phaleria macrocarpa Fruits Extract in Drinking Water on Jejunal Histomorphology of Broiler Chickens N. Ningsih; B. Ariyadi; N. D. Dono; Supadmo Supadmo; Zuprizal Zuprizal
Tropical Animal Science Journal Vol. 42 No. 2 (2019): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.43 KB) | DOI: 10.5398/tasj.2019.42.2.106

Abstract

The purpose of this study was to investigate the characteristics of nanoencapsulated Phaleria macrocarpa fruits extract (NEPM) in drinking water and its effect on microbial population and histomorphology in the jejunal wall of broiler chickens. A total number of 200 male broiler chicks were distributed into 5 treatments with 4 replicates (10 birds in each replicate). The experimental treatments were control diet (T0; negative control), diet with tetracycline (T1; positive control), diet with 2.5% of Phaleria macrocarpa fruits extract (T2), diet with 2.5% NEPM (T3), and diet with 5.0% NEPM (T4). The diet was yellow corn and soybean meal that contains 20.44% CP, 2917.47 kcal/kg ME, 0.84% Ca, and 0.51% Pav. Variables evaluated were characteristics of NEPM, growth performance, intestinal microbial population (lactic acid bacteria (LAB) and Salmonella sp.), and intestinal histomorphology (villus height, crypt depth, and villus height to crypt depth ratio (VH : CD)). Data were analyzed using ANOVA in a completely randomized design. Orthogonal contrast test were used to separate mean of data when p-value differ significantly. Results showed that the size of NEPM was 778 nm with spherical shape and positive charges with the zeta potentials of +26.5 mV. Supplementing 5% of NEPM did not affect broiler growth performance, Salmonella sp. or crypt depth, but increased (p<0.05) LAB, villi height, and VH:CD. It can be concluded that 5.0% of NEPM in the drinking water had positive effect on the jejunal histomorphology and increased population of LAB while Salmonella sp. was not detected on all treatments.
Performance, Microbial Populations, and Jejunal Morphology of Broilers Supplemented with Nano-Encapsulated Graviola Leaf Extract U. Maesaroh; N. D. Dono; Zuprizal
Tropical Animal Science Journal Vol. 45 No. 1 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.1.64

Abstract

This research was conducted to investigate the effects of adding nano-encapsulated graviola (Annona muricata Linn.) leaf extract (NGLE) to drinking water on microbial populations, jejunal morphology, and growth performance of broilers. A total of 300 seven-day-old Lohmann male broilers were allocated into 6 treatments with 5 replications and 10 chicks in each replicate pen. All birds were given the same basal diet but given drinking water treated with: drinking water only as a negative control (T1), drinking water + 25 mg/L Tetracycline (T2), drinking water + 15 mL/L GLE (T3), drinking water + 30 mL/L GLE (T4), drinking water + 15 mL/L NGLE (T5), or drinking water + 30 mL/L NGLE (T6). Variables observed in the current study included: body weight gain (BWG), final body weight (FBW), feed conversion ratio (FCR), feed intake (FI), carcass percentage, jejunal lactic acid bacteria (LAB), jejunal coliform bacteria, villus height and width, crypt depth, and crypt depth ratio. All variable data were statistically analyzed using a completely randomized design with one-way arrangement. Results showed that the addition of NGLE in drinking water improved (p<0.05) jejunal morphology, jejunal LAB, and growth performance of broiler chickens. The height of jejunal villus and population of jejunal LAB increased (p<0.01) when NGLE up to a dose of 15 mL/L was added into the drinking water. Supplementing 15 mL/L NGLE reduced (p<0.01) feed conversion ratio and improved (p<0.01) final body weight and carcass production compared with the other treatments. It is concluded that supplementation of 15 mL/L NGLE might be useful as an alternative for antibiotics growth promoters in poultry.
Intestinal Health in Broiler Chickens Treated with Nanoencapsulation of Terminalia catappa Leaf Extract as an Antibacterial Agent N. A. Hidayati; Zuprizal; Kustantinah; R. E. Indarto; E. Suryanto; N. D. Dono
Tropical Animal Science Journal Vol. 45 No. 4 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.4.443

Abstract

This study aimed to observe the characteristics of nanoencapsulation of Terminalia catappa leaf extract (NETLE) in drinking water as an antibacterial agent and its effects on growth traits, intestinal microflora population, and intestinal micromorphology in broiler chickens. In this study, as many as 192 male broiler chickens were kept in a semi-closed house and distributed into six treatments with four replications, each replication consisting of eight birds, with the experimental treatments consisting of water without any additive (T0; negative control), water added with 50 ppm antibiotics tetracycline (T1; positive control), water added with 15 mL/L T. catappa leaf extract (T2), water added with 30 mL/L T. catappa leaf extract (T3), water added with 15 mL/L NETLE (T4), water added with 30 mL/L NETLE (T5). The diet consisted of yellow corn and soybean meal with 22.09% crude protein, 3155.05 kcal/kg metabolizable energy, 1.10% calcium, and 0.67% available phosphorus. The study showed that the size of NETLE was 77.2 nm with a Polydispersity Index of 0.417 and a zeta potential value of +44.8 mV. It proved that NETLE had antibacterial activity against Escherichia coli, Salmonella typhimurium, and Lactobacillus acidophilus. The administration of NETLE in drinking water did not affect growth performance, villus width, and crypt depth, yet reduced Salmonella sp. population (p<0.01) and increased lactic acid bacteria population (p<0.01), villus height (p<0.01), and the ratio of villus height to crypt depth (p<0.05). The findings showed the beneficial function of NETLE additions in drinking water to improve histomorphology and reduce pathogens in the intestinal of broiler chickens.
Growth Performance and Intestinal Health of Broiler Chickens Supplemented with Coriander Oil Nanoemulsion in Drinking Water K. Sholiha; N. D. Dono; B. Ariyadi; Zuprizal
Tropical Animal Science Journal Vol. 46 No. 1 (2023): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2023.46.1.55

Abstract

This research was conducted to investigate the effects of coriander (Coriandrum sativum) essential oil nano emulsion (NCSEO) in drinking water on growth performance and intestinal health (intestinal microbiota and histomorphology) of broiler chickens. A total of 180 one-day-old male broiler chickens were randomly allocated into five treatments with four replicates and 9 chicks per replicate pen. Birds were provided NCSEO via drinking water. The treatments applied were as follows: without any addition (T0), drinking water added with 50 ppm antibiotic tetracycline (T1), drinking water added NCSEO at 25 μL/L (T2), 50 μL/L (T3), and at 100 μL/L (T4). Variables observed were characteristics of NCSEO, growth performance, intestinal microbiota (LAB and Salmonella spp.), and histomorphology of intestine (villus height, villus width, crypt depth, villus height to crypt depth ratio). All collected data were analyzed using ANOVA in a completely randomized design. Results showed that the particle size of NCSEO was 13.1 nm with PDI 0,257, zeta potential -5.65 mV, stable, and spherical shape. The addition of NCSEO improved (p<0.05) final body weight, weight gain, and FCR, while no significant differences in feed intake and water intake, increased the LAB population (p<0.05) whereas Salmonella spp. was not found, and stimulated (p<0.05) villus height and width. The addition of NCSEO at 50 μL/L resulting the highest promoting effect on growth performance, intestinal microbiota, and intestinal histomorphology of broiler chickens. It can be concluded that 50 μL/L of NCSEO as an alternative to antibiotics can be supplemented in drinking water to improve the growth performance and intestinal health of broiler chickens.
Health Status of Broiler Chickens Fed Diets Containing Palm Kernel Cake with Enzyme Mixture Supplementation Zubaidah, S.; Ariyadi, B.; Hanim, C.; Baskara, A. P.; Zuprizal
Tropical Animal Science Journal Vol. 47 No. 4 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.4.465

Abstract

Palm kernel cake (PKC) utilization as poultry feed has limitations due to its high crude fiber and non-starch polysaccharides, which make it difficult for poultry to digest. This study aimed to determine the effect of enzyme supplementation on blood profile, gastrointestinal health, and intestine histomorphology of broiler chickens fed PKC. This study used 1,080 Indian River strain roosters and two kinds of enzyme mixtures. Enzyme 1 consisted of mannanase 182 g/ton, NSPase 200 g/ton, and protease 130 g/ton, and Enzyme 2 consisted of mannanase 182 g/ton, NSPase 400 g/ton, and protease 260 g/ton. This study used six treatments and six replicates, and each replicate consisted of 30 birds. Data were analyzed using a completely randomized design with a factorial pattern of 2 × 3 and 6 replications. The treatment of factor A consisted of 3 levels of enzyme addition (No enzyme, Enzyme 1, and Enzyme 2), and factor B consisted of 2 levels of PKC (10% and 20%). The data obtained was analyzed using analysis of variance (ANOVA), and the significant difference among treatments was further analyzed using Duncan’s multiple range test. The experimental broilers fed PKC supplemented with enzyme had increased (p<0.05) villus height in the duodenum and ileum and decreased (p<0.05) relative weight percentage of the ventriculus (A2B1 and A3B1). The PKC supplementation increased (p<0.05) the relative weight percentage of proventriculus, duodenum, ileum, caecum, pH of ileum, crypta depth in the duodenum and ileum, but decreased (p<0.05) villus height in the jejunum. Enzyme supplementation increased (p<0.05) the plasma albumin, pH of duodenum, and villus width, but decreased crypta depth in the duodenum. This research concluded that Indian River strain roosters fed ration with 10% PKC supplemented with mannase, NPSase, and protease at doses of 182 g/ton, 200 g/ton, and 130 g/ton had the best health status.
Co-Authors . Supadmo A. Darwoko A. Kurniawati A. M. Chagneau A. Subhan A.S. Nugraha Agus Hadi Prayitno Ali Agus Ali Mas&#039;adi Ali Mursyid W. M. Ali Mursyid Wahyu Mulyono Amrullah, Yusuf Amri Andri Kusmayadi Ari Kusuma Wati Arif Ismail Ariyadi, B. Astriana Napirah B. Ariyadi B. Ariyadi Bambang Ariyadi Bambang Ariyadi Baskara, A. P. Baskara, Aji Praba Besse Mahbuba We Tenri Gading C. Hanim Cahya Setya Utama Cahya Setya Utama Chusnul Hanim Cuk Tri Noviandi D Hardini Daniel Happy Putra Dina Oktaviana Dini Hardini Dini Hardini Dini Julia Sari Siregar Dwi Sulistiawati E. Indarto E. Suryanto E. Tugiyanti Edi Suryanto Edwin Indarto Elly Tugiyanti Eni Siti Rohaeni Eny Sholikhatin Fatima Indah Hayati Ferry Poernama Ferry Poernama H. Sasongko Hardi Julendra Hari Eko Irianto Harimurti Februari Trisiwi Harwi Kusnadi Heni Suryani Heru Ponco Wardono Hery Widijanto Hestu Widyatmoko Hesty Natali Himmatul Khasanah I. Widiyono Indratiningsih (Indratiningsih) Ismoyowati ISTNA MANGISAH Jafendi Hasoloan Purba Sidadolog Jamhari (Jamhari) K. Sholiha Khusnan Khusnan Kusmayadi, A. Kustantinah Kustantinah Kustantinah Kustantinah Kustantinah Lisnahan, Charles V. M. Hidayat M. Kamal M. Larbier M. Nur Cahyanto M. Nur-Cahyanto Muhammad Dani Muhammad Dani Muhammad Hidayat Muhammad Nur Cahyanto N. A. Hidayati N. D. Dono N. D. Dono N. D. Dono N. Ningsih Nanung Danar Dono Nasroedin (Nasroedin) Niati Ningsih Niati Ningsih Niati Ningsih Ning Iriyanti Ning Iriyanti Nur Widodo Nuryono Nuryono R Martien, R R. E. Indarto R. Edwin Indarto R. Martien Rateh Wulandari Ratih Dewanti Riswantiyah (Riswantiyah) Rr. Retno Widyani Rusman Rusman Rusman Rusman Rusman Rusman S Keman, S S. Kismiati S. Kismiati S. Sundari S. Supadmo S. Supadmo Setyono, W. Singgih Hawibowo Sinta Maharani Sinta Maharani Siska Fitria Siska Fitria Siti Zubaidah Soeharto Prawirokusuma Soenarjo Keman SOEPADMO SOEPADMO, SOEPADMO Sri Harimurti Sri Harimurti Sri Purwanti Sri Sudaryati Sunarjo Keman Sundari Sundari Supadmo (Supadmo) Supadmo . Supadmo Supadmo Supadmo Supadmo Supadmo Supadmo Syamsul Arifin Sylvia C. H. Hehanussa T Yuwanta T. Yuwanta T. Yuwanto Taufik Ahmad Zulfian Taufik Ahmad Zulfian Theresia Nur Indah Koni Tri Yuwanta Tri Yuwanta Trijoko (Trijoko) U. Atmomarsono U. Maesaroh Umar Santoso Umi Maesaroh Veithzal Rivai Zainal W.M. Ali-Mursyid Wati, A. K. Wati, Ari Kusuma Wihandoyo (Wihandoyo) Wihandoyo , Wihandoyo Wihandoyo Wihandoyo Wihandoyo, Wihandoyo Wihandoyo, Wihandoyo YUNIANTA YUNIANTA Z. Bachruddin Zaenal Bachruddin Zubaidah, S. Zulfa, I. H.