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TOTAL BACTERIA AND IDENTIFICATION OF PROTEOLYTIC RUMINAL AND RETICULUM BACTERIA FROM LOCAL KACANG GOAT Safika, Safika; Darmawi, Darmawi; Ramadhani, Fitria Sari; Nurhaspika, Nurhaspika; Moliwati, Moliwati
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.9288

Abstract

This study aimed to calculate the total bacteria and to identify the proteolytic bacteria in rumen and reticulum of local Kacang goat. The samples used were the rumen and reticulum fluids of five goats at abattoirs in Banda Aceh. Isolation of proteolytic bacteria was carried out using skim milk agar with pour plate method and incubated at 39 C for 48 hours. The bacterial colonies morphology was observed and the total bacterial count was recorded. DNA of the widest proteolytic index colony was isolated, amplified, and sequenced. The results showed that the dominant colonies morphology was white with position inside the agar. The average of total proteolytic bacteria in goat rumen fluid and reticulum fluid were 6.416x106 CFU/mL and 2,382x107 CFU/mL, respectively. Isolates with the widest proteolytic index was Ru3 (2.5 mm) in the rumen which homology and phylogenetic tree analysis of 16S rRNA showed 93% sequence similarity with Bacillus subtilis, while in reticulum was Re1 (2.0 mm) which has 92% sequence similarity to Tatumella. It is concluded that the number of proteolytic bacteria in reticulum is greater than the number of proteolytic bacteria in the rumen of local kacang goat. Homology analysis in this study proved that the Ru3 and Re1 isolate were probably either a new species or unconfirmed species.
Uji Kekerasan Terhadap Alumunium dan Baja Karbon Rendah Hasil Rendaman Media Konsentrasi Asam Klorida 3% MS, Firdaus; Bayin, Darmawi; Yuliani, Yuliani; Saud, Eddy; Prasetyo, Sugeng; Mukamal, M. Farda Insan
TURBO [Tulisan Riset Berbasis Online] Vol 13, No 1 (2024): TURBO : Jurnal Program Studi Teknik Mesin
Publisher : Universitas Muhammadiyah Metro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24127/trb.v13i1.3285

Abstract

Penelitian ini bertujuan untuk meneliti pengaruh perendaman media Asam klorida konsentrasi 3% terhadap kekerasan Alumunium dan Baja karbon. Bahan yang digunakan dalam penelitian ini adalah baja karbon rendah AISI 1018, aluminium 6061, asam klorida (HCl) 3 %, air. Uji kekerasan menggunakan Mesin Rockwell (HRC type C). Hasil uji kekerasan menunjukan baja karbon rendah lebih keras dibandingkan alumunium. Sebab kedua spesimen memiliki perbedaan struktur mikro dan kepadatannya. Dan juga hasil diatas menunjukan bahwa benda uji yang telah direndam dilarutan HCl tingkat kekerasannya berkurang sebab telah terjadi proses korosi dan pelunakan struktur mikronya.
Diagnosis and medical treatment of otitis externa in a ragdoll cat: A case report Usrina, Nora; Habibie, Maulana; Darmawi, Darmawi; Amiruddin, Amiruddin; Zulkifli, Baidillah; Yusriani, Yenni
The International Journal of Tropical Veterinary and Biomedical Research Vol 8, No 2 (2023): Vol. 8 (2) November 2023
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v8i2.35836

Abstract

Otitis is a disease that is often found due to the cat's ears being unclean and causing earwax to build up along with fluid. Otitis is caused by inflammation of the epithelium of the ear canal and also surrounding structures such as the external auditory meatus and pinna. A 3-month-old Ragdoll cat, male, with white and black hair on the ears, was examined with complaints of head tilting and frequent head shaking, pus in the ears, and inflammation. Clinical examination revealed erythema. The ear wax swab examination results showed an Otodectes cynotis mite infection. The results of the bacterial culture examination showed the presence of Klebsiella sp bacterial infection. The animal was diagnosed with otitis externa. Treatment for otitis externa is given by Dexamethasone, Erlamycetin (Chloramphenicol 1%), Ivermectin, and lidocaine. The accumulated earwax is also cleaned using a cotton bud and saline. Five days after therapy the cat showed recovery.
The Influence of Gadgets on the Morals of Teenagers in Sarolangun Jambi Province Darmawi, Darmawi; Kamaludin, Saman; Angraini, Nani; Suparno, Suparno; Ofiani, Oktawini
Al Qalam: Jurnal Ilmiah Keagamaan dan Kemasyarakatan Vol. 19, No. 6 : Al Qalam (November 2025)
Publisher : Sekolah Tinggi Ilmu Al-Qur'an (STIQ) Amuntai Kalimantan Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35931/aq.v19i6.5115

Abstract

The development of information technology has had a significant impact on the lives of adolescents, including in Sarolangun Regency, Jambi Province. The massive use of gadgets affects various aspects of adolescent life, one of which is morality because morality is a person's behavior or attitude that arises from within, which is based on moral and ethical values. This article aims to analyze the influence of gadget use on adolescent morality in Sarolangun using a qualitative descriptive approach. Data were collected through literature studies from various journals, mass media, and academic books. The results of the analysis show that gadget use has both positive and negative influences on adolescent morality in Sarolangun, Jambi Province. Positive influences include access to educational information and the development of digital skills as well as facilitating access to lessons, while negative influences include decreased social interaction, increased aggressive behavior, and decreased compliance with social norms regarding parents and prevailing social norms in society. This requires the active role of parents, educators, and the community in guiding the wise use of gadgets to shape adolescent morality that is good and beneficial for themselves and society.
Culturally Responsive Humanistic Islamic Education among the Anak Dalam Indigenous Community Darmawi, Darmawi; Warsah, Idi; Fakhruddin, Fakhruddin
Journal La Edusci Vol. 6 No. 5 (2025): Journal La Edusci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallaedusci.v6i5.2627

Abstract

This study explores the implementation of Islamic Religious Education within the Suku Anak Dalam communities of Bukit Duabelas by examining how a humanistic approach enables religious learning to unfold in ways that honor cultural identity and everyday lived experience. Using a qualitative case study design, the research draws on in depth interviews, participatory observations, and document analysis to understand how Islamic teachings are introduced, interpreted, and gradually internalized in communities shaped by semi nomadic movement, strong ancestral traditions, and an oral knowledge system. The findings reveal that religious learning emerges most meaningfully when educators adopt flexible and dialogical strategies such as storytelling, modeling, and culturally grounded conversations that align with the emotional pace and social rhythms of the community. These approaches allow Islamic values to intersect naturally with local wisdom related to solidarity, ecological ethics, and kinship obligations. The study also identifies persistent challenges, including low literacy levels, the enduring authority of adat, and limited access to formal educational resources, which require educators to negotiate teaching with sensitivity and patience. Despite these constraints, observable social transformations indicate growing familiarity with Islamic practice, particularly among children and women. The study concludes that a humanistic and culturally responsive orientation is essential for fostering inclusive and sustainable religious education in indigenous settings and contributes to broader discussions on the development of contextually grounded Islamic pedagogy in multicultural societies
Bioinformatics Analysis of Potential Targets of Betulinic Acid Against Virulence Factors of Chlamydia trachomatis Nurani, Erla; Roza, Putri Diana; Darmawi, Darmawi; Irham, Lalu Muhammad; Adikusuma, Wirawan; Anggraini, Dewi
Jurnal Ilmiah Kesehatan (JIKA) Vol. 7 No. 3 (2025): Volume 7 Nomor 3 Desember 2025
Publisher : Sarana Ilmu Indonesia (Salnesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36590/jika.v7i3.1465

Abstract

Chlamydia trachomatis is an obligate intracellular pathogen responsible for trachoma and sexually transmitted infections, with rising antibiotic resistance posing a major therapeutic challenge. Natural compounds such as betulinic acid, a pentacyclic triterpenoid with broad-spectrum antimicrobial and anti-inflammatory properties, offer potential as alternative therapeutic agents. This study aimed to analyze the potential targets of betulinic acid against C. trachomatis virulence factors using bioinformatics approaches. Protein–compound interaction prediction was performed using STITCH v5.0, while virulence classification was analyzed through VICMpred and VirulentPred. BepiPred v2.0 was employed to identify B-cell epitopes, and PSORTb v3.0 was used to predict subcellular localization. The results identified five proteins targeted by betulinic acid, including DNA topoisomerase IV subunit B (parE), DNA topoisomerase IV subunit A (parC), DNA gyrase subunits (gyrA and gyrB), and sulfite reductase (cysJ). Among these, three were classified as virulence factors: parE (0,2959), parC (0,1754), and cysJ (0,4018). Subcellular localization analysis showed that parE and parC are cytoplasmic proteins essential for DNA replication, while cysJ is associated with the cytoplasmic membrane and metabolic processes. Betulinic acid showed strong potential as an antimicrobial compound targeting key virulence proteins of C. trachomatis. These findings provide foundational insight for further experimental studies to develop betulinic acid–based therapeutic strategies against chlamydial infections.
Cellulase activity of bacteria isolated from water of mangrove ecosystem in Aceh Province Dewiyanti, Irma; Darmawi, Darmawi; Muchlisin, Zainal Abidin; Helmi, Teuku Zahrial; Arisa, Iko Imelda; Defira, Cut Nanda; Fitriyani, Fitriyani; Yura, Sawva
Depik Vol 10, No 3 (2021): December 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.3.22964

Abstract

Cellulolytic bacteria that produce cellulase enzymes play an essential role in degrading cellulose in their habitat. The presence of cellulolytic bacteria strongly supports the fertility and productivity in mangrove waters. The objectives of the study are to analyze the activity of cellulase enzyme qualitatively through the cellulolytic index and quantitatively through the activity and specific activity of the cellulase enzyme from bacteria isolated from the water of mangrove ecosystems in Aceh Province. The qualitative experiment of enzyme activity was carried out at the Microbiology laboratory SKIPM Aceh, and a quantitative experiment of enzyme activity was conducted at the Microbiology Laboratory, Biology Department, IPB. Isolation of cellulolytic bacteria isolated from mangrove water usedCarboxy Methyl Cellulose(1% CMC) selective media and carried out by spread plate method. The ability of bacteria to produce cellulase was tested qualitatively using the spot technique, this test was carried out using 1% Congo Red. Furthermore, the quantitative testing of cellulase enzymes activity adopted the DNS spectrophotometric method. The specific activity of the cellulase enzyme can be determined by using the Lowry method. There were 21 isolates that had a clear zone and had the ability to produce cellulase enzymes from 49 isolates that were successfully purified. The highest cellulolytic index (CI) produced using BAM421 isolate with the value of 5.50 was included in the high category, followed by BAM326 and BAM132 isolates, with values of 1.55 and 1.05 were categorized into the medium category. The other isolates were in the low cellulolytic index category. The isolate with the highest CI value was further tested using the quantitative enzyme activity test. The highest cellulase enzyme activity of BAM421 occurred at 24hr (0.0029 U/ml). The highest specific cellulase activity of BAM421 was at 24hr with the value of 0.210 U/mg. The result concluded that the qualitative test showed CI values can be categorized into low, medium, and high. Moreover, the value of the quantitative assay described that the cellulase enzyme and the specific enzyme activities of the bacteria were low in the study area.Keywords:Cellulolytic indexQuantitative testMangrove watersCellulase enzymeMicroorganismTRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian// TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//
Effect of clean and healthy living behavior (chlb) training on students’ knowledge in marqiz daarul quran wassunnah islamic boarding school, Riau: a quasi-experimental study Winarto, Winarto; Firdaus, Firdaus; Darmawi, Darmawi; Nurkasanah, Siti
Journal of Community Empowerment for Health Vol 9, No 1 (2026)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jcoemph.101890

Abstract

Introduction: Islamic boarding schools (pesantren) face sanitation and behavioral challenges that increase the risk of preventable infectious diseases. Clean and Healthy Living Behavior (CHLB) programs have been promoted nationally; however, limited evidence evaluates the effectiveness of CHLB training on student knowledge in Marqiz Daarul Quran Wassunnah Islamic Boarding School in Kampar, Riau. Therefore, this study aims to evaluate the effect of CHLB training on students’ knowledge at Marqiz Daarul Quran Wassunnah Islamic Boarding School using a one-group pretest–posttest quasi-experimental approach.Methods: This quasi-experimental study involved 62 male students aged 6.5–50 years from Marqiz Daarul Quran Wassunnah Islamic Boarding School in Kampar, Riau. Knowledge was assessed using a validated 15-item questionnaire before and after a 120-minute structured training session. A paired t-test with 95% confidence level was used to compare mean pre- and post-test scores (α = 0.05).Results: Knowledge improved following training, as indicated by a decrease in the mean number of incorrect responses from 3.46 (pre-test) to 1.77 (post-test), with a statistically significant difference (p = 0.0429). However, increased post-test score variability suggested differing levels of understanding among participants.Conclusion: CHLB training significantly improved students' knowledge, though unequal learning gains suggest that a single training session may be insufficient for comprehensive understanding. Integrating recurring training, structured reinforcement, and Poskestren involvement may support long-term behavior change and sustain hygiene practices.
Co-Authors A, Yufrizal Ademi, Gressha Vionalle Aini, Sabrina Ambiyar, Ambiyar Amiruddin Amiruddin Anas, Marnisyah Aneka Firdaus Angraini, Nani Angreini, Meliyantika Annisa, Aiza Apriyan, Dian Arenja, Regina Trisya Arman Sayuti Arsih, Ulfi Widi Arwizet Arwizet, Arwizet Awaluddin Awaluddin Beočanin, Anđelija Budi Syahri budianto panjaitan Chatarina Umbul Wahyuni Cut Nanda Defira, Cut Nanda Dewi Anggraini Dion, Selin Dwinna Aliza Dwisnu, Erlian Erianza, Dava Erwin Erwin Etriwati E Fadhil Fuad Rachman Fakhruddin Fakhruddin Ferdian, Riyan Firdaus Firdaus Fitriyani Fitriyani Giovani, Edo Habibie, Maulana Hasan, Denny Irmawati Hasan, Maryatun Helmi, T. Zahrial Heni Pujiastuti Husni Hidayat Idi Warsah Iko imelda arisa, Iko imelda Irma Dewiyanti Irwin Bizzy Jamil, Kurnian Fitri Kamaludin, Saman Khairussyifa, Najmi Kurnia F. Jamil Kusuma, Herwin Wijaya Lalu Muhammad Irham Liansyah, Tita M. M Amin Indra Putra M. Nur Salim M. Syukri Ismail Maimun Syukri, Maimun Maryanti, Yossi Moliwati, Moliwati MS, Firdaus Mukamal, M. Farda Insan Muslim Akmal N. Nazaruddin Nathania, Auren Ningrum, Sania Devita Nora Usrina Nurani, Erla Nurhaspika, Nurhaspika Nuzul Asmilia Ofiani, Oktawini Pangaribuan, Machyuddin Tumpak Marojahan Prawana, Yogi Ramadhani, Fitria Sari Razali Daud Razali, Renardy Reza Roza, Putri Diana S, Donel Safika S, Safika Saud, Eddy Setiawan, Sepdirama Sibuan, Sibuan Siti Aisyah Siti Nurkasanah Solly Aryza Sugeng Prasetyo Sugito - Suhandri, Wiwin Sukma, Zahwa Tamara Sumanto Sumanto Suparno Suparno Syafruddin Syafruddin Tabrani, Tabrani Taufiq Taufiq Teuku Zahrial Helmi Ummu Balqis Usman, Said Wenny Novita Sari Winarto Winarto Wirawan Adikusuma Wiwit Ade Fidiawati Yenni Yusriani Yuliani Yuliani Yura, Sawva Zahra, Leina Putri ZAINAL ABIDIN MUCHLISIN Zamzami, Rumi Sahara Zikra, Izza Fattalia Zulkifli, Baidillah Zuraidawati Zuraidawati, Zuraidawati