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Hubungan Ekspresi Transforming Growth Factor-β1, Fibroblast Activated Protein, pola Tumor-Associated Collagen Signature dan derajat Tumor-Infiltrating Lymphocytes terhadap Grading International Society of Urological Pathology pada Clear Cell Renal Cell Ca Kharismawaty, Dina; Delyuzar, Delyuzar; Betty, Betty; S Lukito, Joko; Chrestella, Jessy
Majalah Patologi Indonesia Vol. 32 No. 1, Januari 2023
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v32i1.568

BackgroundClear cell renal cell carcinoma (ccRCC) is a very aggressive cancer and is often diagnosed at an advanced stage. The stromal component around the tumor is thought to have contributed greatly to these findings.MethodsAnalytical study with cross sectional design on 23 histological specimens diagnosed by ccRCC based on data from medical records/pathology archives in 2012-2021. Each specimen was stained with hematoxylin and eosin, TGF-β and FAP immunohistochemistry and Masson's trichrome. Fisher's exact test (p<0.05) was used to assess the association between the expressions of all variables with the ISUP grading.ResultsMost cases were high grade ccRCC (87%) samples, of which 73.9% samples expressed strong TGF-β, 82.7% samples expressed positive intratumoral FAP, 78.3% samples expressed positive stromal FAP, 47.8% showed a TACS-3-pattern, 56.5% of samples with positive stromal TILs and 47.8% of samples with negative intratumoral TILs. There was no significant association between all of these variables and the ISUP grading of the ccRCC (p<0.05).ConclusionExpression of TGF-β1, intratumoral and stromal FAP, TACS pattern and the degree of intratumoral and stromal TILs were not significantly associated with ISUP grading in ccRCC.

Hubungan Ekspresi Imunohistokimia CD31 dengan Subtipe Histopatologi, Lymphovascular Invasion dan Angiogenesis pada Papillary Thyroid Carcinoma sinambela, Roy Herbon; Delyuzar; T Kemala Intan
Majalah Patologi Indonesia Vol. 33 No. 2, Mei 2024
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v33i3.634

ABSTRACT Background Thyroid carcinoma is a malignancy of the thyroid gland originating from thyroid follicular cells or parafollicles. The incidence increases slowly and is rare, occurring in approximately 1% of all-body malignancies. Papillary thyroid carcinoma ( PTC ) is influenced by radiation factors, geographic variations, age, and gender. Lymphovascular invasion (LVI) is an independent predictor of lymph node metastases. Angiogenesis is the process of forming new blood vessels and estimating angiogenesis is carried out using a microscopic approach to tumor tissue microvessel density ( MVD ) through CD31 immunohistochemistry . Increased MVD is associated with poor prognosis as an endothelial marker used in calculating MVD using CD31 . Method Cross-sectional analytic study with 32 samples diagnosed with PTC from the Anatomic Pathology Unit of H.Adam Malik General Hospital, Medan. MVD is the number of small blood vessels in terms of size and diameter ( arterioles and venules) with a round lumen stained positively with CD31 which are categorized as low (1-9 blood vessels/10 LPB), moderate (10-19 blood vessels/10 LPB) and high (>20 blood vessels/10 LPB). The relationship of CD31 immunohistochemical expression with histopathological subtypes, LVI and angiogenesis was analyzed by Eta and Somers'd tests . Results There was a significant relationship between CD31 and histopathological subtype (p=0.05 ) , lymphovascular invasion and angiogenesis with a p=0.01 ( p value<0.05 ). Conclusion MVD is significantly associated with histopathological and lymphovascular subtypes . Keywords: CD31 , PTC , LVI , MVD

Hubungan Ekspresi Imunohistokimia Matrix Metalloproteinase-9 dengan Tumor Budding Peritumoral dan Subtipe molekuler pada Invasive Breast Carcinoma of No Special Type Indriani Saragih, Septina; Delyuzar, Delyuzar; Intan, T. Kemala
Majalah Patologi Indonesia Vol. 34 No. 1, Januari 2025
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v34i1.642

Abstract Background Breast cancer ranks first most often in the world and is a malignant cancer that often occurs in women. Matrix metalloproteinase-9 (MMP-9) has been the focus of attention in several previous studies in the field of breast cancer. MMP-9 plays a role in the degradation of the extracellular matrix. High expression of MMP-9 is associated with tumor aggressiveness, metastatic potential and poor prognosis. The presence of tumor budding and molecular subtypes also indicates tumor aggressiveness, one of which is the potential for metastasis through extracellular matrix degradation. The aim of this study was to analyze the association of MMP-9 mmunohistochemistry expression with peritumoral tumor budding and molecular subtypes in IBC-NST. Methods This study is an analytic study with a cross sectional approach on 41 samples of paraffin block cases of IBC-NST which had been diagnosed histopathologically and then performed with MMP-9 mmunohistochemistry staining. The correlation between the immunohistochemical expression of MMP-9 with peritumoral tumor buds and molecular subtypes in IBC-NST was tested statistically using Somers'd test and Eta test. Results There was a significant relationship between the immunohistochemistry expression of MMP-9 with peritumoral tumor budding and molecular subtypes. Conclusion Reporting the results of MMP-9 expression on IBC-NST can be one of the prognostic criteria that can be applied in routine examinations. However, the relationship with prognosis cannot be concluded from this study because it is not associated with survival rate

Correlation between GATA3 Immunohitsochemistry and TP53 on some Ovarium Carcinoma Histopathology Subtype Alamanda, Intan Nefia; Betty, Betty; Delyuzar, Delyuzar; Chrestella, Jessy; Soekimin, Soekimin
Majalah Patologi Indonesia Vol. 34 No. 2, Mei 2025
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v34i3.665

Background Ovarian carcinoma is a cancer with high mortality in women, although comprehensive treatment with surgery and chemotherapy is at an advanced stage, survival rates are still low. GATA3 and p53 are predictors of some malignancies, but results vary in ovarian carcinoma. Objective To examine correlation between immunohistochemical expression of GATA3 and p53 in patients with ovarian carcinoma with various histopathological subtypes. Materials and Methods Cross sectional design is the method of this study conducted on slides of 28 ovarian carcinoma patients in several subtypes of hitopathology. Each slide was stained with hematoxylin and eosin (H&E) to assesses hitopathological subtypes and stained with GATA3 and p53 antibodies. Expression GATA3 was assessed using H-score and p53 quick score. A logistic regression assay (p<0.005) was used to assessed the association of GATA3 and TP53 immunohistochemical expression in several histopathological subtypes of ovarian carcinoma. Statistical analysis between GATA3 and p53 was performed using the eta correlation test is used because the data is nominal-ordinal. Results Among 28 specimens in patients with ovarian carcinoma, Cases was most prevalent in the age group >50-60 years (age range 58 years), history of nullipara parity, and most in the group of stage III ovarian malignancy. Positive immunohistochemical p53 expression is more prevalent in serous carcinoma. Positive GATA3 immunohistochemical expression is more prevalent in serous carcinoma. Conclusion There is no significant relationship. Immunohistochemical expression of GATA3 and TP53 in some histopathological subtypes of ovarian carcinoma. However, immunohistochemical expression of GATA3 high p53 positive tends to be found in high-grade serous carcinoma. Keywodrs: GATA3, p53, karsinoma ovarium, Serous carcinoma, Mucinous carcinoma, Endometrioid carcinoma, Clear cell carcinoma.

Effect of Extraction Method and Solvent Type on Total Phenolics Content, Total Flavonoid and Antioxidant Activity of Pegagan Extract (Centella asiatica (Linn.) Urban) Riza, Ahyar; Syafruddin Ilyas; Endang Sjamsudin; Delyuzar Delyuzar
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 1 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i1.902

Background: Centella asiatica (CA) or pegagan or horse foot leaves is one of the traditional plants widely used to treat various diseases. This study aims to evaluated the content of total phenolics and total flavonoids, as well as the antioxidant activity of Centella asiatica extracts from different methods and solvents. Methods: The sample was divided into three groups, (70% ethanol maceration, methanol maceration and water infundation). Total phenolic compounds in the extract were determined by the Follin-Ciocalteu method, total flavonoid content was determined by the aluminum chloride colorimetric assay method, and antioxidant activity was assessed by the immersion DPPH free radicals method. Determination data of IC50 was assessed by regression test and differences between the three extract groups were tested by ANOVA or Kruskal Wallis test. Results: The highest total phenolics and total flavonoid were obtained in Centella asiatica extract with water solvent, 251.88 ± 0.96 mg/g GAE and 7.26 ± 0.03 mg/g QE, followed by 70% ethanol and methanol. In addition, the best antioxidant activity was also shown in the extract with water, namely 24.09 ± 0.01. The difference test results between all groups showed significant results (p<0.05) in all parameters. Conclusion: The best content of total phenolic, total flavonoid, and antioxidant activity was obtained by infundation with water solvent. In the maceration method, the best content of the three parameters measured was obtained in the group with ethanol solvent.

Effect of Extraction Method and Solvent Type on Total Phenolics Content, Total Flavonoid and Antioxidant Activity of Pegagan Extract (Centella asiatica (Linn.) Urban) Riza, Ahyar; Syafruddin Ilyas; Endang Sjamsudin; Delyuzar Delyuzar
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 1 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i1.902

Background: Centella asiatica (CA) or pegagan or horse foot leaves is one of the traditional plants widely used to treat various diseases. This study aims to evaluated the content of total phenolics and total flavonoids, as well as the antioxidant activity of Centella asiatica extracts from different methods and solvents. Methods: The sample was divided into three groups, (70% ethanol maceration, methanol maceration and water infundation). Total phenolic compounds in the extract were determined by the Follin-Ciocalteu method, total flavonoid content was determined by the aluminum chloride colorimetric assay method, and antioxidant activity was assessed by the immersion DPPH free radicals method. Determination data of IC50 was assessed by regression test and differences between the three extract groups were tested by ANOVA or Kruskal Wallis test. Results: The highest total phenolics and total flavonoid were obtained in Centella asiatica extract with water solvent, 251.88 ± 0.96 mg/g GAE and 7.26 ± 0.03 mg/g QE, followed by 70% ethanol and methanol. In addition, the best antioxidant activity was also shown in the extract with water, namely 24.09 ± 0.01. The difference test results between all groups showed significant results (p<0.05) in all parameters. Conclusion: The best content of total phenolic, total flavonoid, and antioxidant activity was obtained by infundation with water solvent. In the maceration method, the best content of the three parameters measured was obtained in the group with ethanol solvent.

Hubungan Ekspresi CD117 dan Epidermal Growth Factor Receptor (EGFR) terhadap Grading Histopatologi Adenoid Cystic Carcinoma pada Kelenjar Liur Siregar, Ren Astrid Allail; Alferraly, T. Ibnu; Delyuzar
Majalah Patologi Indonesia Vol. 29 No. 1 (2020): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v29i1.402

BackgroundTend to have adenoid cystic carcinoma (AdCC) is one of malignant epithelial tumor of the salivary gland poor prognosis, localrecurrence and metastases. Histopathological grading system according Weert, et al. observed from presence or absence solidcomponent of AdCC. CD117 is a transmembrane tyrosine kinase receptor usually strong expression in AdCC. EGFR belongs to theErbB family of receptor tyrosine kinases associated with cell proliferation, angiogenesis and metastases. Patients with advanceddisease may benefit from targeted therapy besides conventional management. Expression of CD117 and EGFR may give proposaltherapy through difference histology pattern of AdCC based on histopathological grading.MethodsThis is an analytical descriptive study with cross sectional approach. Formalin-fixed paraffin-embedded sections from 35 adenoidcystic carcinomas were obtained for histopathological grading and for immunostained of CD117 and EGFR using monoclonalantibodies. The staining intensity and percentage of cells with membranous and cytoplasmic of CD117 and EGFR expression werescored and then grouped according to the parameters of the Allred scoring system.ResultsChi-square test between CD117, EGFR expression and histopathological grading of AdCC showed no sigficant differences.Besides, McNemar test between CD117 and EGFR expression and histopathological grading AdCC showed significant differences,p-value=0.03 (p<0.05).ConclusionsCD117 and EGFR could be used to detected salivary gland AdCC

Hubungan Ekspresi Sex Determining Region Y Box 2 (SOX2) dengan Grading Histopatologi, Invasi Vaskular Intramural dan Ekstramural pada Adenokarsinoma Kolorektal Rizki, Diana; Delyuzar, Delyuzar; Alferraly, T. Ibnu; Betty, Betty; Soekimin, Soekimin; S. Lukito, Joko
Majalah Patologi Indonesia Vol. 32 No. 2, Mei 2023
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v32i2.617

Background Incidence and mortality of colorectal carcinoma (CRC) worldwide varies widely (10 times). Ninety percent colorectal malignancies are adenocarcinomas. Expression SOX2 is associated with high tumor grade, invasion, metastasis as well as a prognostic predictor of colorectal adenocarcinoma. Objective: Analyze the association of SOX2 immunohistochemistry expression with histopathological grading, intramural and extramural vascular invasion in colorectal adenocarcinoma. Methods Analytical study using a cross-sectional approach on 35 samples from the Anatomic Pathology Unit, H. Adam Malik Hospital, Medan. Immunohistochemical staining of SOX2 mouse monoclonal antibody (Bioassay Technology Laboratory) was performed and expression assessed as low (0-3) and high (4-6). Differences in expression were analyzed by Chi-square and Mann-Whitney correlation tests. Results The most patients with colorectal adenocarcinoma were at age of 51-60 years (34.3%), male sex was more common (60%), the most tumor location was in the rectum (40%), the most depth of invasion was T3 (74.3%) , low grade was more common (60%), more intramural vascular invasion was found (40%), and the highest expression of SOX2 was found to high expression which mostly in the nucleus and cytoplasm (84.6%) There was no significant association between SOX2 expression and histopathological grading (p= 0.884) and there was no significant association between SOX2 expression and vascular invasion, both intramural and extramural (p= 0.390). Conclusion There was no significant association between SOX2 expression and histopathological grading and vascular invasion, both intramural and extramural.

HUBUNGAN DERAJAT TILS INTRATUMORAL DAN STROMAL TERHADAP SUBTIPE TUMOR MUCINOUS OVARIUM DI RSUP H. ADAM MALIK MEDAN: ASSOCIATION BETWEEN INTRATUMORAL AND STROMAL TILS DEGREES AND MUCINOUS OVARIAN TUMOR SUBTYPES AT H. ADAM MALIK GENERAL HOSPITAL MEDAN Kadri, Sayed Muhammad; Delyuzar; Intan, T. Kemala; Soekimin; Lukito, Joko S; Chrestella, Jessy
Jurnal Kedokteran STM (Sains dan Teknologi Medik) Vol. 9 No. 1 (2026): Januari 2026
Publisher : Fakultas Kedokteran Universitas Islam Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/stm.v9i1.987

Mucinous ovarian tumors represent a distinct subtype of epithelial ovarian neoplasms with varying biological behaviors and histopathological features. Tumor-infiltrating lymphocytes (TILs), both intratumoral and stromal, have emerged as prognostic biomarkers in various malignancies, including ovarian cancer. Objective of this study is to analyze the association between intratumoral and stromal TILs degrees with mucinous ovarian tumor subtypes at H. Adam Malik General Hospital, Medan. A cross-sectional analytic study was conducted on 42 formalin-fixed paraffin-embedded tissue samples diagnosed as mucinous ovarian tumors. TILs were assessed on H&E-stained slides using criteria from the International TILs Working Group. Statistical analysis employed Chi-square tests. Results of this study show that high intratumoral TILs were predominantly found in benign mucinous tumors, while low intratumoral TILs were common in malignant cases (p = 0.002). Similarly, high stromal TILs were more frequent in benign subtypes, and low stromal TILs were significantly associated with malignant subtypes (p = 0.000). Conclusion of this study is that there is a significant association between intratumoral and stromal TILs degrees and the histological subtypes of mucinous ovarian tumors. Higher TILs levels are more frequently found in benign tumors, suggesting a potential role as a prognostic indicator. AbstrakTumor ovarium mucinous merupakan salah satu subtipe neoplasma epitel ovarium dengan perilaku biologis dan karakter histopatologi yang bervariasi. Tumor-infiltrating lymphocytes (TILs), baik intratumoral maupun stromal, telah diidentifikasi sebagai biomarker prognostik pada berbagai jenis keganasan, termasuk kanker ovarium. Tujuan penelitian ini adalah untuk mengetahui hubungan antara derajat TILs intratumoral dan stromal dengan subtipe histopatologi tumor mucinous ovarium di RSUP H. Adam Malik Medan. Penelitian analitik potong lintang dilakukan terhadap 42 sampel jaringan tumor mucinous ovarium yang telah difiksasi formalin dan diblok parafin. Penilaian TILs dilakukan pada preparat hematoksilin-eosin (HE) berdasarkan pedoman dari International TILs Working Group. Analisis statistik menggunakan uji Chi-square. Hasil penelitian memperlihatkan bahwa derajat TILs intratumoral tinggi paling banyak ditemukan pada tumor mucinous jinak, sedangkan TILs rendah dominan pada tumor mucinous ganas (p = 0,002). Hal serupa ditemukan pada TILs stromal, di mana TILs tinggi lebih sering pada tumor jinak, dan TILs rendah lebih sering pada subtipe ganas (p = 0,000). Kesimpulan penelitian ini adalah terdapat hubungan bermakna antara derajat TILs intratumoral dan stromal dengan subtipe histopatologi tumor mucinous ovarium. Derajat TILs yang lebih tinggi berhubungan dengan subtipe yang lebih jinak, sehingga berpotensi sebagai indikator prognosis.

The Relationship of Immunohistochemical Expression of BRAFV600E with Clinical Features and Histopathological Subtypes in Papillary Thyroid Carcinoma (PTC) Masriana, Masriana; Betty, Betty; Delyuzar, Delyuzar; Alferraly, Ibnu; Dahlan, Nadjib
Indonesian Journal of Cancer Vol 20, No 1 (2026): March
Publisher : http://dharmais.co.id/

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33371/ijoc.v20i1.1434

Background: Papillary thyroid carcinoma (PTC) is an indolent thyroid malignancy with clinicopathological features that play a role in prognosis. BRAFV600E mutations in PTC are associated with poor prognosis, and BRAFV600E immunohistochemical staining exhibits high specificity and sensitivity. This study aims to determine the association between BRAFV600E immunohistochemical expression and clinical features and histopathological subtypes in PTC.Method: This analytical observational study utilizes a cross-sectional approach, conducted from March to December 2024 at the Anatomical Pathology Laboratory, Faculty of Medicine, Universitas Sumatera Utara, and affiliated hospitals. A total of 35 PTC samples were selected and continued with BRAFV600E immunohistochemical staining. Clinicopathological data, including age, gender, tumor size, lymph nodes, metastasis, lymphovascular invasion, disease stage, and PTC subtype, were collected. Statistical analysis was performed using Somers'd (ordinal-ordinal) and Eta (nominal-ordinal) correlation test in SPSS.Results: PTC was most prevalent among females and the age group under 45 years. Predominant clinical features included tumor sizes exceeding 4 cm (T3), an absence of lymph node involvement (N0), and no detectable metastasis (M0). The classic subtype emerged as the most frequent histopathological variant, typically presenting without lymphovascular invasion (LVI) and diagnosed at Stage I. While most parameters showed no significant statistical association with BRAFV600E expression (p 0.05), a significant relationship was identified specifically regarding the cancer stage (p 0.05).Conclusion: There are no significant associations observed between BRAFV600E immunohistochemical expression and the majority of clinicopathological factors. However, a significant relationship with disease stage was identified. These findings suggest that BRAFV600E expression may have potential value as an indicator of disease stage in PTC.

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