Ketut Adnyane Mudite
Departemen Anatomi, Fisiologi Dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Kampus IPB Darmaga, Jalan Agatis, Bogor 16680 Jawa Barat

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Characterization of Collagen from Swim Bladder Waste of Yellow-pike (Muraenesox talabon) by Acid and Hydrothermal Extraction I Wayan Darya Kartika; Wini Trilaksani; I Ketut Mudite Adnyane
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19 No 3 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (387.531 KB) | DOI: 10.17844/jphpi.v19i3.15074

Abstract

AbstractIndonesian capture fisheries production reached ± 6.4 million tons in 2014. This number was predictedresulted a huge fishery waste, one of them was swim bladder that potentially contain collagen, so it needs tobe developed. This aim of this study was to determine the characteristics of the swim bladder (proportionand chemical content) and collagen characteristics (yield and physicochemical properties) extracted fromthe swim bladder cunang (Muraenesox talabon). Swim bladder waste had proportion 0.57 to 0.67% of wholeyellow-piked conger body weight and 24.74% protein/100 g wet weight (equivalent to 93.39% of protein/100gram dry). The waste had a potency of collagen, which is characterized by the high proportion of theamino acids glycine, proline, hydroxyproline and alain. Acid extraction and hydrothermal process resultedcollagen extracts, identified from a spectrum of amide I, amide II and amide III functional groups. Collagenextracted with acid process contained a higher glycine than hydrothermal one, but instead hydrothermalprocess produced extract with a higher proline, hydroxyproline and alanine composition.
Comparative Microanatomy of The Local Goat and Sheep Pancreas Islets With a Special Reference to The Distribution and Relative Frequency of Glucagon Producing Cells I Ketut Mudite Adnyane; Savitri Novelina; Dwi Kesuma Sari; Tutik Wresdiyati; Srihadi Agungpriyono
Media Veteriner Vol. 8 No. 1 (2001): Media Veteriner
Publisher : Media Veteriner

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Abstract

Penelitian ini memanfaatkan teknik pewarnaan standardan khusus, impregnasi perak Grimelius, untukmenggambarkan morfologi komparasi pankreas kambing dandomba lokal dengan tinjauan khusus pada distribusi danfrekuensi sel-sel penghasil hormon glukagon pada bagianendokrin pankreas. Pankreas domba mempunyai lobulasiyang lebih jelas daripada pankreas kambing ditandai dengansepta interlobaris yang jelas, tetapi batas antara bagianendokrin (pulau Langerhans) dan bagian eksokrin tidak jelaspada domba. Sebaliknya pankreas kambing mempunyaibagian endokrin yang jelas batasnya dengan bagian eksokrin.Pulau Langerhans tersebar diantara eksokrin pankreas,dengan frekuensi terbanyak didapatkan pada pankreas bagiankanan (head), diikuti bagian kiri (tail) dan tengah (body).Pankreas kambing mempunyai bagian endokrin yang lebihbanyak dibanding dengan pankreas domba. Sel-sel penghasilhormon glukagon pada pankreas berbentuk polimorfik, bulat,oval, segitiga atau seperti tetes air dengan butir-butirsitoplasma yang terletak bipolar. Sel-sel ini berdistribusipada bagian perifer dari pulau Langerhans. Jumlah sel-selglukagon berbanding lurus dengan jumlah pulau Langerhanspada pankreas. Perbedaan yang diamati, mencakupperbedaan morfologis, sebaran serta jumlah pulauLangerhans dan sel-sel glukagon, sangat mungkindisebabkan oleh perbedaan dalam jenis dan pola makankedua hewan tersebut.
AH-19 The Heavy Metal Deposit Visualization on Organ Tissues after Slaughtering on Beef Production Adi Winarto; I Ketut Mudite Adnyane; Yohana Paula Prihatmi Pamungkasari
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

The Heavy Metal Deposit Visualization on Organ Tissues after Slaughtering on Beef Production
AH-25 Sperm Morphology of the Javan Muntjak, Muntiacus muntjak muntjak Sri Wahyuni; Gholib Gholib; Wahono Esthi Prasetyaningtyas; I Ketut Mudite Adnyane; Srihadi Agungpriyono; Hamny Hamny; Muhammad Jalaluddin; Mustafa Sabri; Muslim Akmal; Muhammad Agil; Tuty Laswardi Yusuf
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

Sperm Morphology of the Javan Muntjak,  Muntiacus muntjak muntjak
Ah-33 The Effects of Ethanol Mahogany (Sweitenia mahagoni Jacq.) Seeds Extract on Antioxidant Superoxide Dismutase in the Liver Tissues of Diabetic Experimental Rats Eka Prasetiawan; I Ketut Mudite Adnyane; Tutik Wresdiyati
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

The Effects of Ethanol Mahogany (Sweitenia mahagoni Jacq.) Seeds Extract on Antioxidant Superoxide Dismutase in the Liver Tissues of Diabetic Experimental Rats
AH-38 Lectin Histochemistry of the Parotid and Mandibular Glands of Barking Deer, Muntiacus muntjak I Ketut Mudite Adnyane; Md Zuki Abu Bakar; Noordin Mohamed Mustapha; Srihadi Agungpriyono
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

Lectin Histochemistry of the Parotid and Mandibular Glands of  Barking Deer, Muntiacus muntjak
AH-41 Morphology of Mandibularis and Lingualis Glands of Laughting Chicken (Ayam Gaga) with Special Refferences of Distribution and Content of Carbohydrate Muhammad Syukur Hamdan; Novi Susanty; I Ketut Mudite Adnyane; Dwi Kesuma Sari
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

Morphology of Mandibularis and Lingualis Glands of Laughting Chicken (Ayam Gaga) with Special Refferences of Distribution and Content of Carbohydrate
Pemanfaatan α-Tokoferol untuk Meningkatkan Profil Superoksida Dismutase (SOD) Ginjal Tikus di Bawah Kondisi Stres Tutik Wresdiyati; Made Astawan; Diini Fithriani; I Ketut Mudite Adnyane; Mustika Hidayati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v13i3.2568

Abstract

Stress condition has been reported decreased the level of intracellular antioxidant, especially superoxide dismutase (SOD). The condition can lead to certain cell impairment and finally can reach to the degenerative processes, such as aging and carcinogenesis. We need several alternative treatments to solve the severe problem under antioxidant defiency status caused by stress condition. The aim of the study was to observe the effect of α-tocopherol on increasing the profile of superoxide dismutase (SOD) in the kidney of rats under stress condition. The stress condition was achieved by five days fasting together with smimming for 5 min/day and only drinking distiled water ad libitum. α-tocopherol was orally administrated at a dose of 60 mg/Kg/BW/day for seven days. By measuring SOD activity and Cu,Zn-SOD imunohistochemically, showed that α-tocopherol has effect on increasing the profile of intracellular antioxidant in the rats kidney tissue under stress condition.
Pemanfaatan Oleoresin Jahe (Zingiber officinale) untuk Mengatasi Kelainan Antioksidan Intrasel Superoxide Dismutase (SOD) Hati Tikus Di Bawah Kondisi Stres Tutik Wresdiyati; Made Astawan; I Ketut Mudite Adnyane; Renny Candra Prasetyawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2848

Abstract

This study was conducted to evaluate the activity of ginger oleoresin (Zingiber officinale) on the intracelluler antioxidant-superoxide dismutase (SOD) in the liver of rats under stress condition. A total of twuenty five Wistar rats were used for this study. They were divided into five groups ; (1)K (control), (2) S (stress), (3)O + S (oleoresin followed by stress), (4) S + O (stress followed by oleoresin and (5) O + S +O (oleoresin followed by stress then oleoresin). The dose of oleoresin is 60mg/Kg/BW/day for seven days. Stress condition was done by five days fasting and smimming for five minutes/day, while drinking water was provided ad libitum to all groups. The results showed that ginger oleoresin significantly decreased malonaldehyde (MDA) and elevated SOD activity. The immunohistochemical evaluation also showed that ginger oleoresin increased the content of copper,zinc-superoxide dismutase (Cu,Zn-SOD) in the liver of rats under stress condition. These effects were showed in the tissues of rats treated by ginger oleoresin before or after stress or combination of both.
Profil Imunohistokimia Antioksidan Copper, Zinc-Superoxide Dismutase (Cu, Zn, -SOD) pada Ginjal Tikus Perinatal dan Neonatal Tutik Wresdiyati; I Ketut Mudite Adnyane; Silvia Arin Prabandari; Sofiawati Sofiawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2004): October 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.2913

Abstract

The aim of the research was to observe the profile of intracellular antioxidant, especially copper, zinc-superoxide dismutase (Cu, Zn-SOD) in the kidney of perinatal and postnatal rats immunohistochemically. A total of twuelve Wistar rats were used for this research and they were divided into four groups that are 18 days (Fe18) and 20 days old fetus (Fe20), one day (N1) and three days neonatus (N3). There are three rats in each groups. Kidney from each group were taken and processed for routin paraffin embedding methods. The tissues sections were observed with Hematoxylin-eosin (HE) and immunohistochemical technique for Cu, Zn-SOD. Cu,Zn-SOD in the kidney were observed qualitatively in medulla, glomerulus dan cytoplasm of tubuli renalis proximalis and tubuli renalis distails, as well as quantitatively in the nucleus of tubuli renalis cells. The results showed that Cu, Zn-SOD increased in the kidney of perinatal rats, 20 days old fetus (Fe20), then it decreased in the tissues of the one days neonatus (N1), then were increased again in the kidney of three days neonatus (N3) group rats.
Co-Authors . Zuchri A.S. Satyaningtijas Adi Winarto Adisti Dwijayanti Adkhilni Utami Alastair A Macdonald Aldina Safitri Alif Iman Fitrianto Ana Rufaidah Andriyanto Arief Boediono Bibiana W Lay Damiana Rita Ekastuti Diini Fithriani Dwi Kesuma Sari Dwinna Aliza Eka Prasetiawan Eka Prasetiawan Eka Prasetiawan Elmanaviean, Muhammad Elpita Tarigan Fadli A. Gani Fayca Rudhatin Swartidyana Fithriani, Diini Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Gani, Fadli A. Gholib Gholib Gholib Gholib, Gholib Gusdinar, Rizal Hafizuddin Hafizuddin Hamny Sofyan Harry Murti Henny Nuraini Hidayati, Mustika I Ketut Mudite I Ketut Suatha I Wayan Batan Indra Bachtiar Irwandi Jaswir Ita Djuwita Joko Hermanianto Kartika, I Wayan Darya Kelvin Yaprianto Kusdiantoro Mohamad Lidya Elizabeth M. Manik Lisa Dwi Fannessia Lubis, Andri Maruli Tua M Agus Setiadi MADE ASTAWAN Maha, Inggrid Trinidad Mahendra, Arindina Masturi Muhajir Md Zuki Abu Bakar Mokhamad Fahrudin MUHAMMAD AGIL Muhammad Jalaluddin Muhammad Jalaluddin, Muhammad Muhammad Syukur Hamdan Mulyadi Adam Mulyadi Adam, Mulyadi Muslim Akmal Mustika Hidayati Nancy Dewi Yuliana Ni Wayan Kurniani Karja Nining Handhayani Noordin Mohamed Mustapha Novi Susanty Nugraha, Arifin Budiman Nurhidayat Nurhidayat Prabandari, Silvia Arin Prasetyawati, Renny Candra R. Iis Arifiantini Rahmadhani, Elsi Rahmaniyah, Wiwit Ridhani Rakhmawati, Handina Renny Candra Prasetyawati Rudy Priyanto Sandy Qlintang Savitri Novelina Sevy Tiara Ilham Silvia Arin Prabandari Situmeang, Adrian Sofiawati Sofiawati Sofiawati, Sofiawati Sri Wahyuni Sri Wahyuni Srihadi Agungpriyono Sulistyana, Yanti Supar - Supratikno . Tarigan, Ronald Tongku Nizwan Siregar Tutik Wresdiyati Tuty Laswardi Yusuf Tuty Laswardi Yusuf Wahono Esthi Prasetyaningtyas Wahono Esti Prasetyaningtyas Wahyuni, Sri Wasmen Manalu Wini - Trilaksani Wining Astini Wiwit Ridhani Rahmaniyah Yohana Paula Prihatmi Pamungkasari yuri, Nurasmi yuri