Ketut Adnyane Mudite
Departemen Anatomi, Fisiologi Dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Kampus IPB Darmaga, Jalan Agatis, Bogor 16680 Jawa Barat

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Characterization of Collagen from Swim Bladder Waste of Yellow-pike (Muraenesox talabon) by Acid and Hydrothermal Extraction I Wayan Darya Kartika; Wini Trilaksani; I Ketut Mudite Adnyane
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19 No 3 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (387.531 KB) | DOI: 10.17844/jphpi.v19i3.15074

Abstract

AbstractIndonesian capture fisheries production reached ± 6.4 million tons in 2014. This number was predictedresulted a huge fishery waste, one of them was swim bladder that potentially contain collagen, so it needs tobe developed. This aim of this study was to determine the characteristics of the swim bladder (proportionand chemical content) and collagen characteristics (yield and physicochemical properties) extracted fromthe swim bladder cunang (Muraenesox talabon). Swim bladder waste had proportion 0.57 to 0.67% of wholeyellow-piked conger body weight and 24.74% protein/100 g wet weight (equivalent to 93.39% of protein/100gram dry). The waste had a potency of collagen, which is characterized by the high proportion of theamino acids glycine, proline, hydroxyproline and alain. Acid extraction and hydrothermal process resultedcollagen extracts, identified from a spectrum of amide I, amide II and amide III functional groups. Collagenextracted with acid process contained a higher glycine than hydrothermal one, but instead hydrothermalprocess produced extract with a higher proline, hydroxyproline and alanine composition.
Comparative Microanatomy of The Local Goat and Sheep Pancreas Islets With a Special Reference to The Distribution and Relative Frequency of Glucagon Producing Cells I Ketut Mudite Adnyane; Savitri Novelina; Dwi Kesuma Sari; Tutik Wresdiyati; Srihadi Agungpriyono
Media Veteriner Vol. 8 No. 1 (2001): Media Veteriner
Publisher : Media Veteriner

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Abstract

Penelitian ini memanfaatkan teknik pewarnaan standardan khusus, impregnasi perak Grimelius, untukmenggambarkan morfologi komparasi pankreas kambing dandomba lokal dengan tinjauan khusus pada distribusi danfrekuensi sel-sel penghasil hormon glukagon pada bagianendokrin pankreas. Pankreas domba mempunyai lobulasiyang lebih jelas daripada pankreas kambing ditandai dengansepta interlobaris yang jelas, tetapi batas antara bagianendokrin (pulau Langerhans) dan bagian eksokrin tidak jelaspada domba. Sebaliknya pankreas kambing mempunyaibagian endokrin yang jelas batasnya dengan bagian eksokrin.Pulau Langerhans tersebar diantara eksokrin pankreas,dengan frekuensi terbanyak didapatkan pada pankreas bagiankanan (head), diikuti bagian kiri (tail) dan tengah (body).Pankreas kambing mempunyai bagian endokrin yang lebihbanyak dibanding dengan pankreas domba. Sel-sel penghasilhormon glukagon pada pankreas berbentuk polimorfik, bulat,oval, segitiga atau seperti tetes air dengan butir-butirsitoplasma yang terletak bipolar. Sel-sel ini berdistribusipada bagian perifer dari pulau Langerhans. Jumlah sel-selglukagon berbanding lurus dengan jumlah pulau Langerhanspada pankreas. Perbedaan yang diamati, mencakupperbedaan morfologis, sebaran serta jumlah pulauLangerhans dan sel-sel glukagon, sangat mungkindisebabkan oleh perbedaan dalam jenis dan pola makankedua hewan tersebut.
AH-19 The Heavy Metal Deposit Visualization on Organ Tissues after Slaughtering on Beef Production Adi Winarto; I Ketut Mudite Adnyane; Yohana Paula Prihatmi Pamungkasari
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

The Heavy Metal Deposit Visualization on Organ Tissues after Slaughtering on Beef Production
AH-25 Sperm Morphology of the Javan Muntjak, Muntiacus muntjak muntjak Sri Wahyuni; Gholib Gholib; Wahono Esthi Prasetyaningtyas; I Ketut Mudite Adnyane; Srihadi Agungpriyono; Hamny Hamny; Muhammad Jalaluddin; Mustafa Sabri; Muslim Akmal; Muhammad Agil; Tuty Laswardi Yusuf
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

Sperm Morphology of the Javan Muntjak,  Muntiacus muntjak muntjak
Ah-33 The Effects of Ethanol Mahogany (Sweitenia mahagoni Jacq.) Seeds Extract on Antioxidant Superoxide Dismutase in the Liver Tissues of Diabetic Experimental Rats Eka Prasetiawan; I Ketut Mudite Adnyane; Tutik Wresdiyati
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

The Effects of Ethanol Mahogany (Sweitenia mahagoni Jacq.) Seeds Extract on Antioxidant Superoxide Dismutase in the Liver Tissues of Diabetic Experimental Rats
AH-38 Lectin Histochemistry of the Parotid and Mandibular Glands of Barking Deer, Muntiacus muntjak I Ketut Mudite Adnyane; Md Zuki Abu Bakar; Noordin Mohamed Mustapha; Srihadi Agungpriyono
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

Lectin Histochemistry of the Parotid and Mandibular Glands of  Barking Deer, Muntiacus muntjak
AH-41 Morphology of Mandibularis and Lingualis Glands of Laughting Chicken (Ayam Gaga) with Special Refferences of Distribution and Content of Carbohydrate Muhammad Syukur Hamdan; Novi Susanty; I Ketut Mudite Adnyane; Dwi Kesuma Sari
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Abstract

Morphology of Mandibularis and Lingualis Glands of Laughting Chicken (Ayam Gaga) with Special Refferences of Distribution and Content of Carbohydrate
Pelacakan Kerusakan Akrosom Spermatozoa Domba Selama Proses Pembekuan dengan Teknik Histokimia Lektin (DETECTION OF ACROSOMAL DAMAGE OF RAM SPERMATOZOA DURING FREEZING PROCESS USING LECTIN HISTHOCHEMICAL TECHNIQUE) Lisa Dwi Fannessia; Ni Wayan Kurniani Karja; I Ketut Mudite Adnyane; Mohamad Agus Setiadi
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Freezing process in ram spermatozoa caused damage of the plasma membrane and acrosome thatlead to the decrease of spermatozoa fertility. Research was conducted to evaluate acrosomal damageduring freezing process using lectin histochemical technique. Semen was collected twice a week usingartificial vagina from 1-2 years old Garut ram. Immediately after collection, characteristic of semenquality was evaluated then diluted with Niwa and Sasaki Freezing (NSF) medium. Semen was loadedinto 0.25 mL mini straws and equilibrated at 4oC for two hours. Straws were then frozen and stored inliquid nitrogen. Evaluation of sperm characteristic (motility, viability and plasma membrane integrity)and acrosomal integrity were done during the freezing process. Detection of acrosomal integrity was observedusing Fluorescens isothiocyanate (FITC) and Avidin-Biotin-Complex (ABC) staining methods. Data ofcharacteristic spermatozoa and acrosomal integrity were analyzed using ANOVA. Result of the experimentsshowed that the percentage of motility, viability and plasma membrane integrity of spermatozoa before freezing (83 ± 2.7%; 88.8 ± 2.6%; 88.2 ± 3.7%) were significantly decreased (P<0.05) after equilibration (71± 4.2%; 84.2 ± 5.0%; 76.2 ± 1.3%) and after thawing (40 ± 3.5%; 61.08 ± 3.3%; 51.2 ± 10.4%). The percentageof intact acrosomal spermatozoa using FITC and ABC methods during freezing process were 93.63 ±2.73%; 88.04 ± 3.2% and 81.73 ± 4.77% VS 94.54 ± 0.26%; 88.17 ± 0.38% and 79.38 ± 2.06%, respectively.In conclusion, the characteristic of spermatozoa were significantly decrease (P<0.05) during freezing process.Furthermore, the integrity of acrosome spermatozoa can be well analyzed during freezing process by usinglectin histochemical staining methods.
Karakteristik Fisik dan Mikrostruktur Otot Semitendinosus pada Sapi Lokal dan Sapi Impor (PHYSICAL AND MICROSTRUCTURE CHARACTERISTICS OF SEMITENDINOSUS MUSCLE IN LOCAL CATTLE AND IMPORTED COWS) Aldina Safitri; Rudy Priyanto; I Ketut Mudite Adnyane; Henny Nuraini
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.603 KB) | DOI: 10.19087/jveteriner.2018.19.4.488

Abstract

Semitendinosus muscle is widely used as raw material for traditional culinary in Indonesia. Studies on local beef cattle potency have been widely conducted, however there is limited information on physical and microstructures characteristics of semitendinosus muscle. This study aimed to examine physical and microstructures characteristics of semitendinosus muscle from different breeds of local and import beef cattle. This study used semitendinosus muscle from angus cross cattle, bali cattle, brahman cross cattle, PO cattle, and simmental X PO cattle, with age ranging from 18-30 months (I1-I2). The results showed that semitendinosus muscle of local breed cattle could meet the criteria of physical characteristics of consumer demand in Indonesia. Based on perimysium thickness and fascicle area of the cross section of semitendinosus muscle, angus cross cattle and bali cattle had softer muscle texture compared to the other breeds.
Profil Gonad Kodok Lembu Betina yang Diberi Human Chorionic Gonadotropin dan Ekstrak Hipofisis Kodok Lokal I Ketut Mudite Adnyane; Sevy Tiara Ilham; Muhammad Agil
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bulllfrog (Rana catesbeiana) are naturally reach sexual maturity at the age of 3 years aftermetamorphosis. This research aims to study the gonad growth of female bullfrog. given human chorionicgonadotropin (hCG) and hipofise extract of local frog in order to accelerate the maturity. There were 40female bullfrog used in this study and divided into four treatment groups and one control. The treatmentswere injections of 150 IU hCG, 200 IU hCG, 8 hipofise, 16 extract of hipofise and controls. The injectionswere conducted once a month for four months. Every month after the first injection, two of bullfrogs fromeach group were measured their body and gonad weight, in addition, the stage of ovary was also observedeither in macroscopically and microscopically. The results showed that hCG treatment group gave betterresults compared with the treatment of hipofise extract of local frog. The injection of 200 IU hCG gave thebest result of gonad maturity index (IKG) 4.9%, compared with other treatment groups. Giving a dose of200 IU hCG and 16 hipofise exctract of local frog can be used to accelerate the maturity of female bullfrog
Co-Authors . Zuchri Adi Winarto Adisti Dwijayanti Adkhilni Utami Alastair A Macdonald Aldina Safitri Alif Iman Fitrianto Ana Rufaidah Arief Boediono Bibiana W Lay Diini Fithriani Dwi Kesuma Sari Dwinna Aliza Eka Prasetiawan Eka Prasetiawan Elmanaviean, Muhammad Elpita Tarigan Fadli A. Gani Fayca Rudhatin Swartidyana Fithriani, Diini Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Gholib Gholib Gusdinar, Rizal Hafizuddin Hafizuddin Hamny Sofyan Henny Nuraini Hidayati, Mustika I Ketut Suatha I Wayan Batan Indra Bachtiar Irwandi Jaswir Ita Djuwita Joko Hermanianto Kartika, I Wayan Darya Kelvin Yaprianto Kusdiantoro Mohamad Lidya Elizabeth M. Manik Lisa Dwi Fannessia M Agus Setiadi MADE ASTAWAN Maha, Inggrid Trinidad Masturi Muhajir Md Zuki Abu Bakar Mokhamad Fahrudin MUHAMMAD AGIL Muhammad Jalaluddin Muhammad Syukur Hamdan Mulyadi Adam Muslim Akmal Mustika Hidayati Nancy Dewi Yuliana Ni Wayan Kurniani Karja Nining Handhayani Noordin Mohamed Mustapha Novi Susanty Nugraha, Arifin Budiman Prabandari, Silvia Arin Prasetyawati, Renny Candra R. Iis Arifiantini Rahmaniyah, Wiwit Ridhani Renny Candra Prasetyawati Rudy Priyanto Sandy Qlintang Savitri Novelina Sevy Tiara Ilham Silvia Arin Prabandari Sofiawati Sofiawati Sofiawati, Sofiawati Sri Wahyuni Srihadi Agungpriyono Supar - Supratikno . Tongku Nizwan Siregar Tutik Wresdiyati Tuty Laswardi Yusuf Tuty Laswardi Yusuf Wahono Esthi Prasetyaningtyas Wahono Esti Prasetyaningtyas Wahyuni, Sri Wini - Trilaksani Wining Astini Wiwit Ridhani Rahmaniyah Yohana Paula Prihatmi Pamungkasari