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All Journal Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Jurnal Pengolahan Hasil Perikanan Indonesia Media Veteriner Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Mikologi Indonesia JURNAL KAJIAN VETERINER Jurnal Kedokteran Hewan
Ketut Adnyane Mudite
Departemen Anatomi, Fisiologi Dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Kampus IPB Darmaga, Jalan Agatis, Bogor 16680 Jawa Barat
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Veterinary

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Penyuntikan Human Wharton’s Jelly Mesenchymal Stem Cells terhadap Perbaikan Fungsi Testis pada Tikus Tua Fisiologis (HUMAN WHARTON’S JELLY MESENCHYMAL STEM CELLS INJECTION AMELIORATE TESTICULAR FUNCTION ON PHYSIOLOGICAL AGING MALE RATS) Alif Iman Fitrianto; Adkhilni Utami; Wining Astini; Adisti Dwijayanti; Frans Dhyanagiri Suyatna; Kelvin Yaprianto; Indra Bachtiar; I Ketut Mudite Adnyane; Arief Boediono
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The most common therapy on men who suffered fertility decline due to aging was called “T Therapy”, but that’s therapy has long-term risks of sexual dysfunction, metabolic syndrome, prostate, and cardiovascular system. Stem cells are an alternative therapy can be used for ameliorate testicular fuction because of their ability to differentiate into various cell types. The aim of this study was to evaluate the injection of hWJ-MSC in physiologic aging male rats on testicular function. This study was used 3 young male rats (8-12 weeks) and 6 physiological aging male rats (22-24 months) which divided into 3 groups, (i) the young rats, (ii) physiological aging male rats, and (iii) physiological aging male rats that injected with hWJ-MSCs. The young rat group did not give any treatment, physiological aging male rats received NaCl (0.9%) 0.4 mL, and the treatment group received 1x106 cells/kg BW of hWJ-MSCs. The observations were performed on the macroscopical and histological analysis. The result indicates that the younger group had the lowest body weight (154.6 g) and the percentage of the testis weight on the body weight was highest (2.2%) compared to the other groups (P>0.05). The physiological aging rats group had the smallest tubule (9726.9 ìm2) with a largest interstitial area (1117.1 ìm2) compared the other groups (P>0.05). After injection of hWJ-MSC, the tubule area became wider followed by narrowing of the interstitial area (P>0.05). The difference in the body weights is due to the different age of the rat. Improvement of tubule area and interstitial area due to the ability of hWJ-MSCs to improve spermatogenic cells within the tubule. Injection of hWJ-MSCs has been shown to increase fertility in aging rats.
Kebuntingan Hasil Transfer Blastosis Mencit yang Dibekukan dengan Metode Vitrifikasi Kriolup I Wayan Batan; I Ketut Suatha; Ita Djuwita; Nining Handhayani; Wahono Esti Prasetyaningtyas; Ketut Adnyane Mudite; Bibiana W Lay; Supar -; Arief Boediono
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to assess the viability of vitrified embryo using cryoloop as a carrier ofembryo. The blastocyst stage embryos were collected from superovulated mice. Embryos were frozenusing vitrification method and vitrified embryos were loaded on copper filament cryoloop before dipped inliquid nitrogen. The viability of vitrified embryos was assess in vitro by medium cultered and in vivo bytransfered them to recipient mice. The result shows the viability of vitrified embryos was 85,7% after 24hours cultured and the embryos were born from two pregnant recipient mice out of nine (22%) or fouroffspring out of 63 trasfered embryos (6%). In conclusion, vitrified blatocyst stage embryos using cryoloopas a carrier could keep the viability of the embryos and they could be transfered to the recipient mice andwere born normally.
SEL PENGHASIL LISOZIM TERDETEKSI PADA KELENJAR LUDAH SAPI DENGAN TEKNIK IMUNOHISTOKIMIA LYZOZYME PRODUCING CELLS ARE DETECTED IN THE BOVINE SALIVARY GLAND BY IMMUNOHISTOCHEMICAL TECHNIQUE I Ketut Mudite Adnyane; Savitri Novelina; Tutik Wresdiyati; Adi Winarto; Srihadi Agungpriyono
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Lisozim bersifat bakteriolotik dengan cara merusak ikatan glikosida pada dinding bakteri sehingga pelacakan sel penghasil lisozim pada kelenjar ludah sapi perlu dilakukan. Dalam penelitian ini, sel penghasil lisozim diperiksa menggunakan teknik pewarnaan histologi dan imunohistokimia. Hasil penelitian menunjukkan bahwa kelenjar parotis sapi bersifat serous murni. Kelenjar submandibularis bagian asinarnya terdiri atas sel-sel serous dan sel-sel mukous. Sel-sel asinar mukous terlihat lebih dominan jika dibandingkan dengan sel-sel asinar serous. Sel-sel asinar kelenjar parotis sapi bereaksi positif dengan intensitas sedang (++) terhadap pewarnaan imunohistokimia. Sel-sel epitel duktus kelenjar bereaksi sedang (++) sampai dengan kuat (+++). Pola sebaran lisozim yang terdeteksi pada penelitian ini menunjukkan kemiripan dengan hewan mamalia pada umumnya. Sel-sel asinar serous pada kelenjar submandibularis memberikan reaksi positif dengan intensitas rendah (+) sampai dengan sedang (++) dan sel-sel epitel duktus kelenjar dengan intensitas kuat (+++). Lisozim ditemukan pada bagian sel-sel asinar serous dan sel-sel epitel duktus kelenjar parotis dan submandibularis.
Studi Mikroanatomi Pankreas Kodok Lembu Menggunakan Metode Pewarnaan Baku dan Immunohistokimia (MICROSCOPICAL STUDY OF PANCREAS OF BULLFROG USING CONVENTIONAL AND IMMUNOSTAINING METHODS) I Ketut Mudite Adnyane; Supratikno .; Adi Winarto; Srihadi Agungpriyono
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Morphology, distribution and relative frequency of endocrine cells in the pancreas Bullfrog (Ranacatesbeiana) were studied using conventional and immunohistochemical methods. Samples pancreas takenfrom ten adult Bullfrogs (five males and five females). In general, pancreas of the Bullfrog consists ofexocrine portion, endocrine portion (Langerhans islets) and ducts. The Langerhans islets were distributedamong the exocrine portion of pancreas. Endocrine cells in the pancreas of Bullfrog were polimorph, round,oval or triangular in shapes with bipolar cytoplasmic granules. Glucagon cells were distributed mainly inthe peripheral, insulin cells in the center while the somatostatin cells in the area between glucagon andinsulin cells of Langerhans islet. The number of the glucagon cells were higher compare to the number ofinsulin and somatostatin cells.
Perpanjangan Usia Tikus Tua Setelah Xenotransplantasi Human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) Elpita Tarigan; Adisti Dwijayanti; Frans Dhyanagiri Suyatna; Indra Bachtiar; Sandy Qlintang; I Ketut Mudite Adnyane; Arief Boediono
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.261 KB) | DOI: 10.19087/jveteriner.2020.21.4.539

Abstract

Currently, mesenchymal stem cells (MSCs) for implementing regenerative medicine in aging become interest in medical research science, especially in degerative disease and other aging problems. This research was aimed to determine the effectiveness of hUC-MSCs on inhibiting the aging process through the lifetime of the rat and the effect of intravenous administration of hUC-MSCs in phisiologycally aging female rat on the blood analysis. This study was used 40 aged female rats with 29-30 months of age divided into four groups with 10 rats each. The control rat group was given physiological NaCl (0.9%) 0.5 mL, and the treated rat group was given hUC-MSCs 1x107 cells/kg body weight in 0.5 mL NaCl 0.9%, wasinjected intravenously in caudo lateralis tail vein with stratified frequency; one time injection (SC1), three times injections (SC3) and five times injections (SC5). Perifer blood was collected from retro-ortbital sinus vein 30 days before and after injection of hUC-MSCs for hematology and blood chemistry analysis. Based on the results were obtained, it indicated that hUC-MSCs increased the survival of aging rat werein treatment group, life span of rats was extended up to 40 months compared to the average life of control rat aged up to 34±2 months. The injection of hUC-MSCs 1x107 cells/kg of body weight with one, three andfive times injection were affected to blood profiles and blood chemistry with correlation were low. The conclusions are hUC-MSCs extend the lifespan of aging rat and were affect the blood in general but in normal range of aging rat, affect in ALT and creatinin as tissue repair and tolerated by aging rat.
Pemanfaatan α-Tokoferol untuk Meningkatkan Profil Superoksida Dismutase (SOD) Ginjal Tikus di Bawah Kondisi Stres Tutik Wresdiyati; Made Astawan; Diini Fithriani; I Ketut Mudite Adnyane; Mustika Hidayati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v13i3.2568

Abstract

Stress condition has been reported decreased the level of intracellular antioxidant, especially superoxide dismutase (SOD). The condition can lead to certain cell impairment and finally can reach to the degenerative processes, such as aging and carcinogenesis. We need several alternative treatments to solve the severe problem under antioxidant defiency status caused by stress condition. The aim of the study was to observe the effect of α-tocopherol on increasing the profile of superoxide dismutase (SOD) in the kidney of rats under stress condition. The stress condition was achieved by five days fasting together with smimming for 5 min/day and only drinking distiled water ad libitum. α-tocopherol was orally administrated at a dose of 60 mg/Kg/BW/day for seven days. By measuring SOD activity and Cu,Zn-SOD imunohistochemically, showed that α-tocopherol has effect on increasing the profile of intracellular antioxidant in the rats kidney tissue under stress condition.
Pemanfaatan Oleoresin Jahe (Zingiber officinale) untuk Mengatasi Kelainan Antioksidan Intrasel Superoxide Dismutase (SOD) Hati Tikus Di Bawah Kondisi Stres Tutik Wresdiyati; Made Astawan; I Ketut Mudite Adnyane; Renny Candra Prasetyawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2848

Abstract

This study was conducted to evaluate the activity of ginger oleoresin (Zingiber officinale) on the intracelluler antioxidant-superoxide dismutase (SOD) in the liver of rats under stress condition. A total of twuenty five Wistar rats were used for this study. They were divided into five groups ; (1)K (control), (2) S (stress), (3)O + S (oleoresin followed by stress), (4) S + O (stress followed by oleoresin and (5) O + S +O (oleoresin followed by stress then oleoresin). The dose of oleoresin is 60mg/Kg/BW/day for seven days. Stress condition was done by five days fasting and smimming for five minutes/day, while drinking water was provided ad libitum to all groups. The results showed that ginger oleoresin significantly decreased malonaldehyde (MDA) and elevated SOD activity. The immunohistochemical evaluation also showed that ginger oleoresin increased the content of copper,zinc-superoxide dismutase (Cu,Zn-SOD) in the liver of rats under stress condition. These effects were showed in the tissues of rats treated by ginger oleoresin before or after stress or combination of both.
Profil Imunohistokimia Antioksidan Copper, Zinc-Superoxide Dismutase (Cu, Zn, -SOD) pada Ginjal Tikus Perinatal dan Neonatal Tutik Wresdiyati; I Ketut Mudite Adnyane; Silvia Arin Prabandari; Sofiawati Sofiawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2004): October 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.2913

Abstract

The aim of the research was to observe the profile of intracellular antioxidant, especially copper, zinc-superoxide dismutase (Cu, Zn-SOD) in the kidney of perinatal and postnatal rats immunohistochemically. A total of twuelve Wistar rats were used for this research and they were divided into four groups that are 18 days (Fe18) and 20 days old fetus (Fe20), one day (N1) and three days neonatus (N3). There are three rats in each groups. Kidney from each group were taken and processed for routin paraffin embedding methods. The tissues sections were observed with Hematoxylin-eosin (HE) and immunohistochemical technique for Cu, Zn-SOD. Cu,Zn-SOD in the kidney were observed qualitatively in medulla, glomerulus dan cytoplasm of tubuli renalis proximalis and tubuli renalis distails, as well as quantitatively in the nucleus of tubuli renalis cells. The results showed that Cu, Zn-SOD increased in the kidney of perinatal rats, 20 days old fetus (Fe20), then it decreased in the tissues of the one days neonatus (N1), then were increased again in the kidney of three days neonatus (N3) group rats.
Teknik Memanen Makrokonidia dari Dermatofita Microsporum gypseum dan Trichophyton mentagrophytes Ana Rufaidah; Eko Sugeng Pribadi; I Ketut Mudite Adnyane
Jurnal Mikologi Indonesia Vol 4, No 2 (2020): Desember 2020
Publisher : Perhimpunan Mikologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46638/jmi.v4i2.87

Abstract

Dermatofitosis adalah penyakit kulit yang disebabkan oleh kapang dermatofita. Penelitian ini bertujuan menentukan teknik yang sesuai untuk memanen makrokonidia dan waktu fase eksponensial Microsporum gypseum dan Tricophyton mentagrophytes. Media 1 dan 2 digunakan untuk pembiakan isolat Microsporum gypseum dan Trichophyton mentagrophytes. Media 1 mengandung pepton, dekstrosa, dan kloramfenikol dan pada media 2 memiliki komposisi yang sama dengan media 1 dengan tambahan sikloheksamida. Teknik penggoresan pada permukaan koloni dan penggoyangan tabung dengan vortex digunakan untuk memanen makrokonidia. Makrokonidia dipanen pada hari ke 10, 12, 14, 16, 18, dan 20. Hasil yang didapatkan menunjukkan jumlah rata-rata makrokonidia kapang yang dipanen dengan penggoresan pada permukaan koloni lebih tinggi daripada penggoyangan tabung menggunakan vortex selama masa pertumbuhan hingga hari ke-20. Hasil yang didapatkan juga menunjukkan fase eksponensial telah dimulai pada hari ke-14.
Differentiation of beef, buffalo, pork, and wild boar meats using colorimetric and digital image analysis coupled with multivariate data analysis Fayca Rudhatin Swartidyana; Nancy Dewi Yuliana; I Ketut Mudite Adnyane; Joko Hermanianto; Irwandi Jaswir
Jurnal Teknologi dan Industri Pangan Vol. 33 No. 1 (2022): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2022.33.1.87

Abstract

Beef price is relatively expensive, which makes this commodity vulnerable to be counterfeited. The development of rapid, cheap and robust analytical methods for meats authentication has therefore become increasingly important. In this study, colorimetric and digital image analysis methods were used to characterize and classify four types of meat (beef, buffalo, pork, wild boar) and two muscle types from each sample (Semitendinosus and Vastus lateralis). Multivariate data analysis (PCA and OPLS-DA) was used to observe classification pattern among species using different color parameters data obtained from meat chromameter and digital image measurement. The results showed that PCA and OPLS-DA successfully classified meat from different species and different muscle type based on color, both in chromameter and in image analysis. It was shown that pork had the highest lightness level, and was the most different among the four types of meat tested. Beef was predominated by yellowish color, while buffalo meat had the highest reddish color level. Semitendinosus and Vastus lateralis muscles had different color intensity where Vastus lateralis exhibited darker color intensity. This study showed that meat color analysis using chromameter and imaging techniques can be used as cheap and quick tools to discriminate meats form different species and different muscles type.
Co-Authors . Zuchri Adi Winarto Adisti Dwijayanti Adkhilni Utami Alastair A Macdonald Aldina Safitri Alif Iman Fitrianto Ana Rufaidah Arief Boediono Bibiana W Lay Diini Fithriani Dwi Kesuma Sari Dwinna Aliza Eka Prasetiawan Eka Prasetiawan Elmanaviean, Muhammad Elpita Tarigan Fadli A. Gani Fayca Rudhatin Swartidyana Fithriani, Diini Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Gholib Gholib Gusdinar, Rizal Hafizuddin Hafizuddin Hamny Sofyan Henny Nuraini Hidayati, Mustika I Ketut Suatha I Wayan Batan Indra Bachtiar Irwandi Jaswir Ita Djuwita Joko Hermanianto Kartika, I Wayan Darya Kelvin Yaprianto Kusdiantoro Mohamad Lidya Elizabeth M. Manik Lisa Dwi Fannessia M Agus Setiadi MADE ASTAWAN Maha, Inggrid Trinidad Masturi Muhajir Md Zuki Abu Bakar Mokhamad Fahrudin MUHAMMAD AGIL Muhammad Jalaluddin Muhammad Syukur Hamdan Mulyadi Adam Muslim Akmal Mustika Hidayati Nancy Dewi Yuliana Ni Wayan Kurniani Karja Nining Handhayani Noordin Mohamed Mustapha Novi Susanty Nugraha, Arifin Budiman Prabandari, Silvia Arin Prasetyawati, Renny Candra R. Iis Arifiantini Rahmaniyah, Wiwit Ridhani Renny Candra Prasetyawati Rudy Priyanto Sandy Qlintang Savitri Novelina Sevy Tiara Ilham Silvia Arin Prabandari Sofiawati Sofiawati Sofiawati, Sofiawati Sri Wahyuni Srihadi Agungpriyono Supar - Supratikno . Tongku Nizwan Siregar Tutik Wresdiyati Tuty Laswardi Yusuf Tuty Laswardi Yusuf Wahono Esthi Prasetyaningtyas Wahono Esti Prasetyaningtyas Wahyuni, Sri Wini - Trilaksani Wining Astini Wiwit Ridhani Rahmaniyah Yohana Paula Prihatmi Pamungkasari