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All Journal Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Jurnal Pengolahan Hasil Perikanan Indonesia Media Veteriner Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Mikologi Indonesia JURNAL KAJIAN VETERINER Jurnal Kedokteran Hewan
Ketut Adnyane Mudite
Departemen Anatomi, Fisiologi Dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Kampus IPB Darmaga, Jalan Agatis, Bogor 16680 Jawa Barat
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Veterinary

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STUDI HISTOKIMIA LEKTIN PADA SEL-SEL SPERMATOGENIK TESTIS MUNCAK (Muntiacus muntjak muntjak) (Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak)) Sri Wahyuni; Srihadi Agungpriyono; I. Ketut Mudite Adnyane; Hamny Hamny; Muhammad Jalaluddin; Gholib Gholib; Muslim Akmal; Mulyadi Adam; Dwinna Aliza; Tongku Nizwan Siregar
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3396

Abstract

The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 µg/ml of concentration for PNA lectins and 15µg/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates β-galactose, β-glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis. Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak
The Quality of Indo-Pacific Bottlenose Dolphin (Tursiops aduncus) Sperm Following Liquid-storage in Low Temperature Fahrudin, Mokhamad; Gusdinar, Rizal; Arifiantini, Raden Iis; Prasetyaningtyas, Wahono Esthi; Adnyane, I Ketut Mudite; Elmanaviean, Muhammad; Nugraha, Arifin Budiman; Karja, Ni Wayan Kurniani
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 4 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.4.456-466

Abstract

Indo-Pacific bottlenose dolphin (Tursiops aduncus) is a marine mammal that lives in relatively small populations. The geographic ranges of this species are susceptible to the effects of human activities, thereby necessitating conservation efforts to prevent extinction. Therefore, this study aimed to evaluate the daily quality of dolphin sperm after several days of refrigeration. The sperm of two male dolphins were stored at 4oC for 4 days, and the quality was observed daily to determine the motility, viability, membrane integrity, and sperm abnormalities. Sperm samples were divided into four groups, consisting of two centrifuged followed by the removal of seminal plasma, and two groups without centrifugation, containing 100x106 and 200x106 sperm/ml each. After liquid storage, the motility of sperm was 63-75% with no significant reduction in the first 3 days. Sperm viability following storage was 65-75% and the percentage with abnormal morphology ranged from 2-6%. Furthermore, there was no significant increase in abnormal morphology of sperm on any day of storage for 3 days. Sperm membrane integrity was 36-49%, with no significant reduction in the membrane integrity in the first 2 days. There was no significant difference in sperm quality, although centrifugation and removal of seminal plasm had a slight effect. The results of this study showed that Indo-Pacific bottlenose dolphin sperm could be stored for a short period as liquid storage while maintaining a quality that allows for future use.
Co-Authors . Zuchri Adi Winarto Adisti Dwijayanti Adkhilni Utami Alastair A Macdonald Aldina Safitri Alif Iman Fitrianto Ana Rufaidah Arief Boediono Bibiana W Lay Diini Fithriani Dwi Kesuma Sari Dwinna Aliza Eka Prasetiawan Eka Prasetiawan Elmanaviean, Muhammad Elpita Tarigan Fadli A. Gani Fayca Rudhatin Swartidyana Fithriani, Diini Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Gholib Gholib Gusdinar, Rizal Hafizuddin Hafizuddin Hamny Sofyan Henny Nuraini Hidayati, Mustika I Ketut Suatha I Wayan Batan Indra Bachtiar Irwandi Jaswir Ita Djuwita Joko Hermanianto Kartika, I Wayan Darya Kelvin Yaprianto Kusdiantoro Mohamad Lidya Elizabeth M. Manik Lisa Dwi Fannessia M Agus Setiadi MADE ASTAWAN Maha, Inggrid Trinidad Masturi Muhajir Md Zuki Abu Bakar Mokhamad Fahrudin MUHAMMAD AGIL Muhammad Jalaluddin Muhammad Syukur Hamdan Mulyadi Adam Muslim Akmal Mustika Hidayati Nancy Dewi Yuliana Ni Wayan Kurniani Karja Nining Handhayani Noordin Mohamed Mustapha Novi Susanty Nugraha, Arifin Budiman Prabandari, Silvia Arin Prasetyawati, Renny Candra R. Iis Arifiantini Rahmaniyah, Wiwit Ridhani Renny Candra Prasetyawati Rudy Priyanto Sandy Qlintang Savitri Novelina Sevy Tiara Ilham Silvia Arin Prabandari Sofiawati Sofiawati Sofiawati, Sofiawati Sri Wahyuni Srihadi Agungpriyono Supar - Supratikno . Tongku Nizwan Siregar Tutik Wresdiyati Tuty Laswardi Yusuf Tuty Laswardi Yusuf Wahono Esthi Prasetyaningtyas Wahono Esti Prasetyaningtyas Wahyuni, Sri Wini - Trilaksani Wining Astini Wiwit Ridhani Rahmaniyah Yohana Paula Prihatmi Pamungkasari