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Cocoa liquor increases SOD activity in wistar rats experiencing oxidative stress Kartika Wahyu Dwi Putra; Bambang Wirjatmadi; Bambang Purwanto; Merryana Adriani
Health Notions Vol 2, No 1 (2018): January
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.472 KB) | DOI: 10.33846/hn.v2i1.98

Abstract

Chocolate is a healthy food. The active ingredient in chocolate (Theobroma cacao L.), especially antioxidants, can counteract the free radicals present in the body. This study aimed to determine the effect of cocoa liquor on increased activity of Superoxyde Dismutase (SOD). The study design was randomized post test only control group with 20 samples of male white rats wistar (Rattus norvegicus). The sample was divided into 4 groups: 2 control groups and 2 treatment groups. The treatments provided were instantaneous physical activity and cocoa liquor preparation with a single dose. This study used one way anova and continued with post hoc tukey HSD test for statistic. The results showed that cocoa liquor had an effect on SOD activity. SOD activity increased in treatment group with cocoa liquor either 2 hours or 24 hours before the momentary physical activity. Keywords: Cocoa liquor, Superoxide dismutase, Oxidative stress
The Effect of Dayak Onion Bulb-Stem (Eleutherine Palmifolia (L.,) Merr.) Extract on Blood Glucose Levels of Mouse Suffered Diabetes Mellitus Niluh Arwati; Bambang Wirjatmadi; Merryana Adriani; Soenarnatalina Meilanani; Dwi Winarni; Sri Hartiningsih
Health Notions Vol 2, No 3 (2018): March
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.309 KB) | DOI: 10.33846/hn.v2i3.153

Abstract

Dayak onions (Eleutherine Palmifolia (L.) Merr.) bulb stem contains phtyochemical contents, which act as antidiabetic compounds, such as eleutherol, eleuthocide A, and eleutherinoside B, as well as antioxidant compounds, which include triterpenoid, poliphenol, and flavonoid. Dayak onions was able to be used as the antidiabetic, since it had the ability to lower the blood glucose level and prevent from the free radicals, thus supressing the oxidative stress condition. This research had purpose to analyze the effect of Dayak onions bulbstem as antioxidant and anti diabetic drugs. The research used experimental method with the population in this research was 25 male white mice strain wistar. The concentration of Dayak onions bulb-stem extracts were 300mg.kgBW-1, 400mg.kgBW-1, and 500mg.kgBW-1. Data analysis used Tukey HSD Test with 95% of significance degree and was continued using manova test (average group ratio test). The result showed that. the extract of Dayak onions bulb-stem had the antidiabetical and antioxidant activity, which could lower the blood glucose levels and malondialdehid on the male white mice strain Wistar with the optimum effective doze of 500 mg.kgBW-1.Keywords: Dayak onions bulb-stem, Blood glucose level, Malondialdehid (MDA)
Effects of Purple Sweet Potatoes on Oxidative Stress Biomarkers in Rats Subjected to Exhaustive Exercise Anindya Mar’atus Sholikhah; Bambang Wirjatmadi; Merryana Adriani
Health Notions Vol 2, No 2 (2018): February
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.618 KB) | DOI: 10.33846/hn.v2i2.117

Abstract

Oxidative stress is a condition caused by the imbalance between the production of free radicals or ROS and the antioxidants; the level of free radicals is higher than the antioxidants. Exhaustive exercise is one of the causes of this oxidative stress. However, it can be reduced by antioxidants found in purple sweet potatoes (PSP) (Ipomoea batatas L). This study aims to evaluate the effect of two weeks supplementation of PSP extract on rats inducedoxidative stress. Twenty rats were distibuted into four groups consisted five rats each. Rats in the treatments groups were administered PSP extract for two weeks then were forced to swim until exhausted. Bloods from all samples were drawn to see the changes in the levels of malondialdehyde ans superoxide dismutase after two weeks of treatment. The data was then analyzed using descriptive and inferential statistic. The results showed that mean level of MDA and SOD differ significantly (p<0.05) between groups after the administration of PSP extract as well as after exhaustive exercise. The extracts and exhaustive exercise concurrently showed a significant effect on oxidative stress biomarkers (p<0.05). It concluded that PSP extract can protect cells against free radical produced by exhaustive exercise. Keywords: Purple sweet potatoes, Physical activity, Oxidative stress, MDA, SOD
The Role of Nutritional Status, Dietary Pattern and Free Radical in the Insulin Resistance Development Meirna Dewita Sari; Bambang Wirjatmadi; Merryana Adriani
Health Notions Vol 1, No 4 (2017): October-December
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.009 KB) | DOI: 10.33846/hn.v1i4.79

Abstract

Insulin resistance is an early sign of diabetes type 2 that occurs in situations where target organ sensitivity decreases against insulin. Excess free radicals can be mutually insulin resistance. The purpose of this study was to determine how the effects of insulin can be a determining factor. This study used case control design conducted on 22 outpatients at Clinic University of Muhammadiyah Surabaya during June until July 2017. Data were collected using questionnaires, nutritional status, fasting blood glucose, fasting insulin, and MDA levels. Insulin resistance status using normal HOMA IR method of <2.77. Logistic Regression was used to determine the effect of free radical on the occurrence of insulin resistance. The results showed free radical against insulin resistance at =0.05 with p=0.018. That result means boosting MDA levels also increases the risk of insulin resistance. In addition, the consumption of dietary sources of fat and excessive use of food additives, low fruit intake, BMI values are also factors affecting insulin resistance with p=0.027; p=0.036; and p=0.027. Given the elevated levels of MDA-free radicals can increase the risk of insulin resistance. Applying a healthy lifestyle by consuming fiber-rich foods can prevent the formation of excessive free radicals. Keywords: Insulin resistance, Free radical, HOMA IR
The Influence of Sinbiotic Yogurt on Microscopical Frequent Using Microscopes on the Wistar Rats (Rattus norvegicus) Jayanthi Petronela Janggu; Bambang Wirjatmadi; Merryana Adriani
Health Notions Vol 1, No 3 (2017): July-September
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn.v1i3.38

Abstract

Escherichia coli are a gram-negative bacteria belonging to the normal human intestinal flora. Excessive type of E.coli pathogen bacteria could cause infectious disease for humans. In the gastrointestinal tract, especially in the small intestine, the pathogen that often causes interference is Enteropathogenic Escherichia coli. Growth of pathogenic bacteria could be inhibited by administration of sinbiotic yogurt. Synbiotic yogurt is a yogurt that is supplemented with probiotics and prebiotics. Probiotics used were Lactobacillus bulgarius, Streptococcus thermophiles, Bifidobacterium bifidium with the addition of prebiotics i.e. purple sweet potato containing oligosaccharides. The aims of this study was to saw the effect of giving sinbiotic yogurt to microscopic images of the small bowel of wistar rats. This research was a laboratory experimental research. The subjects consisted of 24 wistar rats divided in the control group (6 tails) and the treatment group (18 tails) divided into 3 groups with 6 each group. The results of group I with histologic features of normal intestinal tissue, group II partial epithelial mucosal epithelium seen erosion, group III with epithelial cell surface epithelial mucosa appear normal and group IV with epithelial surface erosion but, cell regeneration was characterized by increasing goblet cells . The administration of sinbiotic yogurt given E. coli had a good effect of improving the epithelial cells of the small intestine surface by increasing goblet cells.Keywords: Escherichia coli, Sinbiotic yogurt, Small intestinal mucosa
Influence of Cinnamon Extract (Cinnamomum burmanii) Provision on the Reduction of Blood Glucose Level of Hyperglycemic Male Wistar Rats Kharina Aulia; Bambang Wirjatmadi; Merryana Adriani
Health Notions Vol 2, No 3 (2018): March
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (805.397 KB) | DOI: 10.33846/hn.v2i3.150

Abstract

The number of patients with hyperglycemia in the world and in Indonesia is estimated to increase rapidly in the next 25 years. The content of cinnamaldehyde, flavonoids, polyphenols, and chromium in cinnamon thought to have an influence of hypoglycemia. This study aimed to determine the influence of cinnamon extract to a decrease of blood glucose levels in hyperglycemic rats induced by a single dose of alloxan as much as 125 mg/kg. This study was laboratory experiment by using RAL (Complete Randomize Design) with Pre-Post Test Control Group Design Research. Experimental animals used were male Wistar rats divided into 5 groups. KN was negative control group. KP was hyperglycemic positive control group. P1 was the first treatment group (a dose of 200 mg/kg). P2 was the second treatment group (a dose of 250 mg/kg). P3 was the third treatment group (a dose of 300 mg/kg). Cinnamon therapy was given orally for 14 days. The results of this research was tested statistically by using One Way ANOVA and showed that there were significant differences between the blood glucose levels in the treatment group with the positive and negative control groups. The conclusion was that the provision of cinnamon extract for 14 days could provide an influence of lowering blood glucose levels. Keywords: Blood glucose, Cinnamon, Hyperglicemia
Bee Pollen Effect on Blood Glucose Levels in Alloxan-induced Male Wistar Rats Aprilia Nurcahyaning Rahayu; Bambang Wirjatmadi; Merryana Adriani; Soenarnatalina M.; Dwi Winarni; Sri Hartiningsih
Health Notions Vol 2, No 1 (2018): January
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.122 KB) | DOI: 10.33846/hn.v2i1.85

Abstract

Bee pollen, as an active ingredient in diabetes mellitus (DM) prevention and treatment, is able to reduce oxidative reactions and to regenerate cells. This study aimed to determine bee pollen effect on blood glucose levels of rats which induced by alloxan. This study used a completely randomized design with Pre-Post Test design and Control Group design. The subjects were 25 male wistar rats which divided randomly into 5 groups: (1) negative control group (non-DM rats); (2) positive control group (DM rats without bee pollen); (3) P1 group (DM rats with 0.25 g/day bee pollen); (4) P2 group (DM rats with 0.63 g/day bee pollen); and (5) P3 group (DM rats with 1.25 g/day bee pollen). Alloxan inducing was used to cause rats under DM or hyperglycemic conditions. The data were analyzed by Manova test and Tukey HSD test. Statistical analysis showed that bee pollen effectively had an effect on decreasing fasting blood glucose (FBG) levels in treatment groups with 0.002. Differences test showed a difference between positive control group and treatment groups, but there was no difference between all treatment groups. However, the bee pollen dosage which giving the lowest average of FBG was 0.25 g on P1 group. Keywords: Bee pollen, Blood glucose level, Alloxan
Expression of VCAM in Male Wistar Rats (Rattus norvegicus) with Hypercholesterolemia Supplemented with Pumpkin Seeds (Cucurbita moschata Duch) Extract Jujuk Proboningsih; Bambang Wirjatmadi; Kuntoro Kuntoro; Merryana Adriani
Health Notions Vol 2, No 6 (2018): June
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (970.221 KB) | DOI: 10.33846/hn.v2i6.214

Abstract

Treatment of cardiovascular diseases tends to be expensive and requires long-term therapy. Investigation about the effect of pumpkin seeds on prevention of endothelial dysfunction of blood vessel has never been reported yet, accordingly, bodies of research are required to know its effectiveness. This study aimed to analyze the expression of Vascular Cell Adhesion Molecule (VCAM) in male Wistar rats (Rattus norvegicus) with hypercholesterolemia supplemented with pumpkin seeds extract (Cucurbita moschata Duch). This was an experimental laboratory study with randomized post-test control group design. The independent variable was pumpkin seeds extract, and the dependent one was expression of VCAM. Data analysis employed chi-square test to test the difference between control and treatment groups. Study results showed that there was significant difference (p=0.000) between VCAM expression in group-K2 (positive control) as compared to group-K1 (negative control) and group-K5 (treatment group-dose 3) α=0.001, whereas no significant difference was found between that of as compared to group-K3 (treatment group-dose 1) α=0.125 and group-K4 (treatment group-dose 2) α=0.05. The study showed reduction of VCAM expression in treatment groups consisted of male Wistar rats with hypercholesterolemia supplemented with pumpkin seeds (Cucurbita moschata Duch) extract. Keywords: Pumpkin seeds, VCAM expression, hypercholesterolemia
Ekstrak Kelopak Rosella Merah (Hibiscus sabdariffa Linn.) Mampu Memperbaiki Histopatologi Hepar Tikus Wistar yang Diberi Paparan Asap Rokok Arya Ulilalbab; Bambang Wirjadmadi; Merryana Adriani
Jurnal Kesehatan Andalas Vol 6, No 3 (2017)
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v6i3.760

Abstract

Prevalensi penduduk di Indonesia usia ≥ 15 tahun yang merokok mengalami peningkatan. Asap rokok merupakan salah satu sumber radikal bebas. Tujuan penelitian ini adalah menganalisis pengaruh ekstrak kelopak rosella merah terhadap degenerasi hepatosit tikus wistar yang diberi paparan asap rokok. Total sampel 20 tikus jantan dan dibagi menjadi empat kelompok: perlakuan ekstrak kelopak rosella merah dosis 540 mg/bw, ekstrak kelopak rosella merah dosis 270 mg/bw, kontrol positif dan kontrol negatif. Kelompok perlakuan diberi pakan standar + ekstrak rosella dipagi hari dan setelah itu dipapar 2 rokok per hari. Kontrol positif diberi pakan standar + dipapar 2 rokok per hari. Kontrol negatif hanya diberi pakan standar. Penelitian dilakukan selama 28 hari. Diakhir penelitian, diamati gambaran histopatologi hepar. Pemberian ekstrak kelopak rosella merah dosis 270 mg/kg bb dan dosis 540 mg/kg bb dapat mencegah degenerasi hepatosit. Analisis degenerasi hepatosit dengan uji Kruska  Wallis pada   p<0,05 dan dilakukan uji lanjut Mann-Whitney. Simpulan studi ini ialah semua perlakuan dapat mencegah, bahkan bisa memperbaiki degenerasi hepatosit tikus wistar yang diberi paparan asap rokok.
MEDIA PENDIDIKAN GIZI NUTRITION CARD BERPENGARUH TERHADAP PERUBAHAN PENGETAHUAN MAKANAN JAJANAN ANAK SEKOLAH DASAR Ni Putu Wahyuningsih; Siti Rahayu Nadhiroh; Merryana Adriani
Media Gizi Indonesia Vol. 10 No. 1 (2015): JURNAL MEDIA GIZI INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (189.016 KB) | DOI: 10.20473/mgi.v10i1.26-31

Abstract

Less knowledge related to street food among primary schoolchildren is one of problem that need an attention. An effort to tackle that problem is crucial, for example by giving nutrition education through educational media. This research aims to analyze differences in knowledge after intervention with nutrition card and whiteboard. The design study used was quasi experimental with pre test – post test control group. Sample in this study was fifth grade student with 27 primary schoolchildren in each group. Intervention was conducted for 1 month consist of 8 meetings and each meeting was done for over 30 minutes. Based on mann whitney test, result shows that there was a differences in knowledge (P<0,05) after nutrition education with a nutrition card. The conclusion from this study is nutrition card could knowledge about food street among primary schoolchildren. Wider socialization of this type of education is need to be done both in schoolchildren in SDN Keputih 245 as well as other schools.Keywords: nutrition card, nutrition education, primary school children
Co-Authors Abdillah, Bagus Ahmad Nur Abu Bakar Achmad Ali Basri Ade Saputra Nasution Aditia Rida Purnamasari Agus Sulistyono Ajeng Putri Rahmandita Amanda Nurqisthy Anindya Mar’atus Sholikhah Anisatun Azizah Annis Catur Adi Annisa Rachma Firdausi Darmawan Aprilia Nurcahyaning Rahayu Arinda Lironika Suryana Arwati, Niluh Arwati, Niluh Arya Ulilalbab Astuti, Nanik Dwi Aulia, Kharina B Wirjatmadi Bambang Purwanto Bambang Purwanto Bambang Purwanto Bambang Wiratmadi Bambang Wirjadmadi Bambang Wirjatmadi BAMBANG WIRJATMADI Bambang Wirjatmadi Bambang Wirjatmadi Bambang Wirjatmadi Bambang Wirjatmadi Bambang Wirjatmadi Bambang Wirjatmadi Brigita Rainy Oktiva Candra Panji Asmoro, Candra Panji Cici Damayanti D.A. Liona Dewi Denna Rahinda Yulia Fanni Desy Prasetya Desy Prasetya, Desy Dewi Retno Suminar Dini Ririn Andrias Dini Setiarsih Dwi Putra, Kartika Wahyu Dwi Winarni Elida Ulfiana Fajar Afrindo Fara Disa Durry Farapti Farapti Fatifa Asmarani Fatifa Asmarani, Fatifa Fika Mayrlina Anggrayni Haryana, Nila Reswari Hermadani, Candraningtyas Ika Nur Pratiwi Ikhram Hardi S Indarwati , Retno Intan Galih Cornia Jayanthi Petronela Janggu Jayanthi Petronela Janggu Jujuk Proboningsih, Jujuk Junaida Astina Kartika Wahyu Dwi Putra Kharina Aulia Kuntoro Kuntoro Kuntoro Kuntoro Kusnanto Kusnanto Kusnanto Kusnanto Laila Nurayati Lailatul Muniroh Lailatun Ni&#039;mah Laily Hidayati Lestari, Annisa Arifiana Lury Novita Yustianingrum M. Dodi F Priaminda M., Soenarnatalina Mahmud Aditya Rifqi Maria Widijanti Sugeng Meirna Dewita Sari Muhammad Dimas Bimantara Mustika Putri Kustiyoasih Nanik Dwi Astuti Nathania Helsa F. Losong Ni Putu Wahyuningsih Nina Agustina Novera Herdiani, Novera Nur Nashriana Jufri Nursalam Nursalam Nursalam, Nursalam Oktovina Rizky Oktovina Rizky, Oktovina Pei-Lun Hsieh Priaminda, M Dodi F Pudji Astutik Pudji Astutik, Pudji Puspita Ayu Ramadhani Rachmah, Qonita Rachmat Hargono Raharja, Kristian Triatmaja Rahayu, Aprilia Nurcahyaning Rahmi Syarifatun Abidah Rahmi Syarifatun Abidah, Rahmi Syarifatun Ratna Sari Rumakey Reineldis Trisnawati Rifka Laily Mafaza Rina Wasesa Aprilia Lazulfa Rista Fauziningtyas, Rista Rivan Virlando Suryadinata Rivan Virlando Suryadinata Rivan Virlando Suryadinata Rizka Amalia Habiba Rosita Andriani Santi Kusumastuti Setho Hadisuyatmana Setiarsih, Dini Sheila Monica Kelly Amalia Sheryll Deby Regyna Sholikhah, Anindya Maratus` Sholikhah, Desty Muzarofatus Shrimarti Rukmini Devy Silviana Putri Siti Nur Qomariah Siti Rahayu Nadhiroh Soenarnatalina M. Soenarnatalina Meilanani Soenarnatalina Meilanani, Soenarnatalina Sri Hartiningsih Sri Hartiningsih Sri Hartiningsih Stefani Cornelia Sardjono Susanti, Yuyun Erlina Susanti, Yuyun Erlina Susila Rusdiana Dewi Tia Eka Novianti Tri Martiana Tri Ratna Ariestini Triharini, Mira Triska Susila Nindya Wijatmadi, Bambang Windhu Purnomo Wiratmadi, Bambang Yayuk Estuningsih Yeni Susanti