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Desain Habitat Buatan Kura-kura Batok (Cuora amboinensis) di Area Konservasi Eksitu Universitas Bengkulu Saputra, Fajar Eko; Karyadi, Bhakti; Parlindungan, Deni; Sutarno, Sutarno; Ruyani, Aceng; Indriyanti, Nurma Yunita
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11097

Abstract

Cuora amboinensis has experienced a population decline of 50–80 percent in CITES Appendix II since 2000 and is currently endangered (EN) or threatened with extinction, so it is important to preserve it. Bengkulu University's ex-situ conservation involves the preservation of Sumatran turtles obtained from collectors in the Bengkulu area. This research aims to design an artificial habitat in an effort to conserve Cuora amboinensis This research method uses an ecological-based design. The research subject was Cuora amboinensis Data collection methods include field observations, literature studies, discussions with turtle conservation experts, and monitoring environmental conditions. The research instrument uses environmental sensors to measure abiotic data. Data analysis was carried out descriptively with systematic research and analysis with field findings supported by previous researchers' findings and equipped with documentation images and a table presentation of environmental sensor data. The research results explain the stages in designing an artificial habitat for Cuora amboinensis, namely site selection and design, fencing and security, adaptation and acclimation, dividing areas based on function and providing supporting biota, nest building, and monitoring environmental conditions. The conclusion of this research Overall, this habitat design provides an optimal environment for Cuora amboinensis so that it can be used as a reference for conservation.
Giving a combination of snails (Achatina fulica) and coconut dregs on the growth and protein content of maggots (Hermetia illucens) Purnama, Intan; Ruyani, Aceng; Yani, Ariefa Primair; Johan, Henny; Parlindungan, Deni; Nursa’adah, Euis
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica, Vol. 12: No. 2 (August, 2025)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v12i2.22660

Abstract

This study aims to determine the effect of providing a combination of snails and coconut dregs as feed on the growth and protein content of Black soldier Fly (BSF) maggots. The method used is an experiment with a Completely Randomized Design (CRD) consisting of five treatments and three replications. The treatments consist of: P1 (100% snails), P2 (75% snails: 25% coconut dregs), P3 (50%: 50%), P4 (25%: 75%), and P5 (100% coconut dregs). Maggot maintenance was carried out for 14 days with feeding carried out every 2 days in the morning at 10:00. The research data observed included Weight, Thickness, Length which were analyzed statistically using the ANOVA test and the test was continued with the Least Significant Difference (LSD) test, and the Kruskal wallis test if the data was not normal, temperature and humidity, Feed consumption, Specific growth rate (SGR), Waste reduction index (WRI), and Protein content and water content were analyzed using the automatic kjeldahl test. The results of the study showed that the provision of snail and coconut dregs feed had a very significant effect on the growth of maggots (P <0.05) both in weight and thickness, but had no significant effect (P>0.05) on the growth of maggot length. Treatment P1 (100% snail) gave the best results with an average weight of 0.09 g, a length of 13.4 mm, and the highest protein content of 27.49%. Treatment P5 (100% coconut dregs) showed the lowest results in all main parameters. It was concluded that snail feed was more optimal in supporting the growth and protein content of BSF maggots. The high animal protein content and soft texture are the main reasons, which show the potential of snails as a highly nutritious alternative feed for BSF maggot cultivation. Keywords: BSF Maggots; Coconut dregs; Growth; Snails
Development of Electronic Student Worksheets (E-LKPD) Pendap Bengkulu Based on Discovery Learning as A Learning Media for Additive Material Nutriastuti, Putri; Uliyandari, Mellyta; Parlindungan, Deni; Mayub, Afrizal; Defianti, Aprina
Jurnal Paedagogy Vol. 11 No. 2 (2024): April
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jp.v11i2.11176

Abstract

This research aims to develop the electronic student worksheets (E-LKPD) Pendap Bengkulu based on Discovery Learning as a Learning Media for Additive Material in Science lesson content for class VIII SMP. This study used the 4D development model (Define, Design, Develop, and Disseminate), which was limited to the development stage with limited trials conducted at SMPN 03 Bengkulu City, with a sample of 33 students from class VIII.1. To collect data, several questionnaires were distributed to media experts, material experts, participants, and students. Data analysis was carried out using descriptive statistical analysis techniques using percentage analysis. The results of the research showed that the feasibility test of E-LKPD involving 3 material experts, 2 media experts, and 2 participants resulted in an 92.3% percentage of validation test results, and it can be said that E-LKPD Pendap Bengkulu, which was developed as a learning medium for additive material for junior high school students, was very feasible to use. Analysis of student readability of E-LKPD Pendap Bengkulu as a learning medium for additive material for junior high school students resulted in a percentage of 89.8% readability response, indicating that E-LKPD is very well applied to junior high school students in grade VIII.
Studi Ekologi Makroinvertebrata di Area Kolam Konservasi Ex-situ Kura-Kura Siebenrockiella crassicollis di Universitas Bengkulu Hasbi; Karyadi, Bhakti; Parlindungan, Deni; Uliyandari, Mellyta; Defianti, Aprina
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.9730

Abstract

Makroinvertebrata merupakan organisme tidak bertulang belakang yang memiliki ukuran kasat mata, memiliki habitat di darat, dalam  tanah, perairan meliputi air laut dan tawar. Kelompok makroinvertebrata di perairan bisa dijadikan bioindikator suatu perairan. Makroinvertebrata di perairan air tawar contohnya kelompok keong air tawar, serangga air, udang dan Clitellata seperti lintah. Penelitian dilakukan pada bulan Oktober 2023 sampai Januari 2024 menggunakan teknik purposive sampling. Pengambilan sampel menggunakan leaf litter bags, jaring seser, dan mengambil sedimen dasar perairan menggunakan ember. Pengukuran parameter lingkungan dilakukan meliputi pengukuran intensitas cahaya, suhu air, tingkat keasaman (pH), oksigen terlarut (DO), kedalaman lumpur, dan kedalaman air. Analisis data menggunakan indeks Shannon-Wiener, indeks Eveness dan indeks Simpson. Hasil identifikasi makroinvertebrata menunjukkan terdapat 16 spesies terdiri atas 13 famili dan 4 kelas. Indeks keanekaragaman makroinvertebrata di area kolam konservasi ex-situ kura-kura Siebenrockiella crassicollis Universitas Bengkulu secara umum tekanan ekologis berada pada kondisi tekanan rendah hingga sedang, dapat dilihat pada nilai H’ berkisar antara 0,981 sampai 1,577, indeks keseragaman berkisar antara 0,354 sampai 0,569 dan indeks dominansi berkisar antara 0,342 sampai 0,506. Kelimpahan, keanekaragaman, keseragaman dan dominansi makroinvertebrata dipengaruhi oleh parameter fisika kimia dan kondisi lingkungan. 
Identification of the Diversity of Medicinal Plants Used by Battra in North Bengkulu Fanisah, Kiki; Setiawan, Iwan; Parlindungan, Deni; Karyadi, Bhakti; Defianti, Aprina; Yani, Ariefa Primair
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.3876

Abstract

All kinds of plants that are found in nature and have medicinal properties are considered medicinal plants. Utilization of medicinal plants can be used directly and mixed. Battra is a person who has knowledge of traditional medicine and is capable of concocting medicinal plants. North Bengkulu is one of the districts that still use conventional medical care. Utilizing medicinal plants that are proven to be able to treat those derived from natural ingredients can be healthful because of very few side effects compared to drugs that contain chemicals. The goal of the study was to catalog the various medicinal herbs used by Battra North Bengkulu. This study used 10 respondents' interviews as its approach. Battra uses 64 species from 37 families of medicinal plants as traditional medicinal ingredients. The most common type of family is Fabaceae. The habitus group that is widely used is 45% trees. Types of diseases that are commonly treated traditionally using these plants include fever, toothache, itching, cramps, internal heat, scales, stomach pain, malaria, long-term fever, colds (cholera), ulcers, lungs, kidney stones, jaundice, high blood pressure, appendicitis, fussy children, eye disease, intestinal worms, kesapo, sprains, tumors and gout. In the processing of medicinal plants by Battra in North Bengkulu, 11 processes the highest drug processing method was by boiling as much as 37%.
Pemberian Jus Buah (Etlingera hemisphaerica) terhadap Pemulihan Eritrosit dan Leukosit Akibat Perlakuan Merkuri Klorida (HgCl2) pada Mencit (Mus musculus) Azzahrah, Fatimah; Ruyani, Aceng; Parlindungan, Deni; Listiono, Arsela Eko; Mayub, Afrizal
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 8 No. 5 (2025): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/7ef7hb33

Abstract

This study aims to determine the effect of Etlingera hemisphaerica (honje) fruit juice on the recovery of erythrocyte and leukocyte counts in mice (Mus musculus) exposed to mercuric chloride (HgCl₂). The method used was an experimental design using male mice as test animals, divided into three groups: K1 (control), K2 (honje juice 0.3 mL/20 g BW for 3 days), and K3 (HgCl₂ 5 mg/kg BW + honje juice 0.3 mL/20 g BW for 3 days). The observed parameters were the number of erythrocytes and leukocytes before and after treatment. The results showed that honje juice administration increased the erythrocyte count in group K2, while in group K3, erythrocyte recovery was affected by mercury exposure, making the honje juice less effective, although the values remained within the normal range compared to the control group. In leukocyte count, group K2 showed a stabilizing effect, whereas group K3 experienced an increase due to HgCl₂ exposure. In conclusion, Etlingera hemisphaerica fruit juice has potential as a phytotherapy agent to repair hematological damage caused by heavy metal toxicity, although its effectiveness is influenced by the metabolic condition of the mice and the high concentration of mercury. Keywords: Etlingera hemisphaerica, Erythrocytes, Leukocytes, Mice, Mercuric Chloride
Study of dogania subplana aclimatization in the turtle learning center university of bengkulu Marliansa, Meiredo; Karyadi, Bhakti; Parlindungan, Deni; Ruyani, Aceng; Nursa'adah, Euis
JPBIO (Jurnal Pendidikan Biologi) Vol 10, No 2 (2025): November 2025
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v10i2.4953

Abstract

The forest softshell turtle (Dogania subplana) is a species increasingly threatened by habitat destruction and overexploitation. This study aims to identify the acclimatization process of D. subplana in an artificial environment, specifically, an aquarium at the Turtle Learning Center (TLC). The research was conducted over 30 days from July to August 2024. Observations were made on daily behavior, body temperature, and health condition, individual biometric measurements, and environmental (abiotic) parameters. The results showed that daily behaviors such as feeding, basking, and hiding occurred regularly, indicating the species' ability to adapt to a new environment. The turtles experienced an increase in body weight, while growth in carapace length and width was very slow. The body temperature of D. subplana varied in accordance with the surrounding environmental temperature, reflecting its dependence on ambient conditions. Some individuals experienced minor health issues, such as fungal infections, but the majority remained active and healthy. Environmental factors such as temperature, humidity, pH, dissolved oxygen, total dissolved solids, and light intensity were within ranges conducive to acclimatization. These findings suggest that the aquarium at the TLC is quite effective as an ex situ conservation facility in supporting the adaptation process of D. subplana  outside its natural habitat.
Reduction lead metal in process marinating local food fish pendap using lemon juice (citrus limon) Asti, Nora Ayudi; Johan, Henny; Parlindungan, Deni; Primairyani, Ariefa; Defianti, Aprina; Nursaadah, Euis
JPBIO (Jurnal Pendidikan Biologi) Vol 10, No 2 (2025): November 2025
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v10i2.5209

Abstract

Fish have the potential to be contaminated with heavy metals such as lead, which can originate from human activities, industry, and ports. The Bengkulu people have a traditional processing method that is believed to reduce heavy metal content in fish through the immersion of fish in Pendap dishes using water and lemon. This study aims to observe the reduction of heavy metal content in fish during the immersion process using water and lemon juice in Pendap dishes. The research was experimental with positive and negative results and analyzed using the SPSS ANOVA test to see significant differences between treatment groups. The fish used as samples included Tongkol Sarda orentalis, Kembung Rastrelliger kanagurta, Tuna Thunnus albacares, and Tenggiri Scomberomorus Lacepède. The parts of the fish that were analyzed included the muscles, liver, and gills. All samples were exposed to Pb(NO3)2 and divided into 3 treatment groups: 1 positive control, 2 precipitated with water, and 3 precipitated with lemon juice (Citrus limon). The samples were tested using the reagents NaOH, KI, NH4OH, and HCl. Based on the research results, it was found that the application of lemon juice produced significant results in reducing Pb levels, as indicated by the negative test results.
Effect honje forest fruit juice administration on hemoglobin levels following mercury chloride treatment in mice Iskandar, Aiza Sabina Putri; Ruyani, Aceng; Parlindungan, Deni; Johan, Henny; Mayub, Afrizal
JPBIO (Jurnal Pendidikan Biologi) Vol 10, No 2 (2025): November 2025
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v10i2.5561

Abstract

Exposure to mercury chloride (HgCl?) is known to induce oxidative stress, which negatively affects the hematological system, including a decrease in hemoglobin levels. This study aims to analyze the effect of honje fruit juice (Etlingera hemisphaerica), known for its high antioxidant content, on hemoglobin levels in mice (Mus musculus) exposed to mercury chloride. This study used an experimental method involving several groups of mice, namely group K1 (control), group K2 (given honje fruit juice), and group K3 (injected with mercury and given honje fruit juice). Hemoglobin levels were measured using a Sahli tube. The results showed that honje fruit juice significantly increased hemoglobin levels in mice exposed to mercury chloride compared to the control group. These findings indicate that the antioxidant content in honje fruit can help reduce the toxic effects of mercury chloride on the circulatory system. Therefore, honje fruit juice has the potential to be developed as a natural therapeutic agent in efforts to mitigate the toxic effects of mercury chloride.