Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

3.799

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Akuakultur Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Soils BERKALA SAINSTEK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Penelitian Pascapanen Pertanian Indonesian Journal of Biotechnology Jurnal Sumberdaya Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology BIOEDUSCIENCE Jurnal Ilmu Nutrisi dan Teknologi Pakan ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Menara Perkebunan Makara Journal of Science Jurnal Natural Annales Bogorienses Berita Biologi

Anja Meryandini

Biology Department, Faculty Of Mathematics And Natural Sciences, IPB University And Biotechnology Center, Bogor 16680, Indonesia

Author-ID : 229368

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Veterinary

Published : 90 Documents Claim Missing Document

Claim Missing Document

Articles

Title

1 2

Characterization of Xylanase Streptomyces spp. SKK1-8 ANJA MERYANDINI; TRIO HENDARWIN; DEDEN SAPRUDIN; YULIN LESTARI
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Streptomyces spp. SKK1-8 producing xylanase was isolated from soil sample from Sukabumi West Java. The xylanase have an optimum condition at pH 6 and 50 0C. Addition of 5 mM Cu2+ decreased the xylanase activity up to about 77%, whereas not by other cations. The xylanase was stable at 3 0C for 48 hours, and the enzyme half lifetime was 1 hour 45 minute at 50 0C. This xylanase showed the highest activity on oatspelt xylan, and their molecular masses were estimated approximately 16.80, 15.21, and 13.86 kDa. HPLC analysis showed that xylosa and arabinosa were the main hydrolytic product of birchwood xylan. Key words: xilanase, Streptomyces spp., characterization, zymogram and SDS-PAGE, stability

Characterization of Xylanase from Streptomyces spp. Strain C1-3 ANJA MERYANDINI
HAYATI Journal of Biosciences Vol. 14 No. 3 (2007): September 2007
Publisher : Bogor Agricultural University, Indonesia

Xylan is the major constituent of hemi cellulose. Several enzymes are needed to hydrolyse xylan completely, including xylanase. Currently, there is an increasing use of this enzyme. This study was carried out to characterize the xylanase from Streptomyces spp. strain C1-3. Results showed that the xylanase displayed its highest activity at pH 3 and 90 0C and was stable up to 10 hours at this conditions. Its activity increased after the addition of Cu2+, Fe2+, and Co2+ under concentration of 1 and 5 mM, respectively. The activity however, decreased after the addition of Mg2+, Ca2+ at 1 mM and Zn2+ at 5 mM. After a test with five kinds of xylan (i.e. from Birchwood, Beechwood, Arabinoxylan, Oat spelt and CMC), the xylanase of Streptomyces spp. C1-3 showed its preferences to Birchwood- and Arabino-xylan. The results showed that the xylanase of Streptomyces spp. C1-3 was characterized as a thermostable acid xylanase. Key words: xylanase, Streptomyces, stability, CMCase

Yeast Isolation for Bioethanol Production EKA RURIANI; TITI CANDRA SUNARTI; ANJA MERYANDINI
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

We have isolated 12 yeast isolates from five different rotten fruits by using a yeast glucose chloramphenicol agar (YGCA) medium supplemented with tetracycline. From pre-screening assay, four isolates exhibited higher substrate (glucose-xylose) consumption efficiency in the reaction tube fermentation compared to Saccharomyces cerevisiae dan Saccharomyces ellipsoids as the reference strains. Based on the fermentation process in gooseneck flasks, we observed that two isolates (K and SB) showed high fermentation efficiency both in sole glucose and mixed glucose-xylose substrate. Moreover, isolates K and SB produced relatively identical level of ethanol concentration compared to the reference strains. Isolates H and MP could only produce high levels of ethanol in glucose fermentation, while only half of that amount of ethanol was detected in glucose-xylose fermentation. Isolate K and SB were identified as Pichia kudriavzeevii (100%) based on large sub unit (LSU) ribosomal DNA D1/D2 region.

Characterization of Xylanase activity produced by Paenibacillus sp. XJ18 from TNBD Jambi, Indonesia . KURRATAA’YUN; . YOPI; ANJA MERYANDINI
HAYATI Journal of Biosciences Vol. 22 No. 1 (2015): January 2015
Publisher : Bogor Agricultural University, Indonesia

Lignocellulose waste in nature is increasing due to the increasing activity of agroforestry. Up to 40% of lignocellulose biomass are consisted of xylan. Xylan complete breakdown requires the action of xylanase. Xylanase has been used to breakdown xylan into commercial product such as low calories sugar, prebiotic, and biofuel. Due to its wide application, several variation of xylanase characterization are needed. Our previous studies have collected Paenibacillus sp. XJ18 from TNBD forest, Jambi, Indonesia, to gain a unique enzyme characteristic. In this study the characteristic of crude xylanase from Paenibacillus sp. XJ18 was investigated. The highest activity of xylanase production was at 36 h. The xylanase showed activity in a broad range of pH (4.5-9.0). The highest activity showed at pH 5.0, 90 oC. Crude enzyme extract was unstable and had halftime at its pH and optimum temperature about 67 min. The xylanase activity was increased about 4.59 times after being concentrated by 70% acetone (2.4578 U/mL). Based on TLC result, xylanase from Paenibacillus sp. XJ18 was predicted to produce xylobiose exclusively from extracted corncob xylan.

Enzymatic Hydrolysis of Copra Meal by Mannanase from Streptomyces sp. BF3.1 for The Production of Mannooligosaccharides . ARIANDI; . YOPI; ANJA MERYANDINI
HAYATI Journal of Biosciences Vol. 22 No. 2 (2015): April 2015
Publisher : Bogor Agricultural University, Indonesia

Copra meal contained high polysaccharide mannan. Mannanase Streptomyces sp. BF3.1 efficiently hydrolyzed copra meal to mannooligosaccharides. This research determined the optimum conditions of enzyme mannanase Streptomyces sp. BF3.1 to hydrolyze copra meal. The results of the hydrolysis products were analyzed concentrations of reducing sugars, total sugars and the degree of polymerization. In order to determine the type of product, mannooligosaccharides were analyzed by thin layer chromatography and high performance liquid chromatography. The mannanase had an optimum condition at 70 °C and pH 6. Optimum conditions of hydrolysis was 10% copra meal concentration with incubation time of 5 h at 30 °C which able to produce a variety of mannooligosaccharides products. Under such conditions, the yield of reducing sugar was 3.83 mg/mL with polymerization degree of 4. Analysis of mannooligosaccharides by thin layer chromatography and high performance liquid chromatography revealed mannobiose, mannotriose, mannotetrose, mannopentose, and mannoheksose.

Effect of Probiotic Bacillus megaterium PTB 1.4 on the Population of Intestinal Microflora, Digestive Enzyme Activity and the Growth of Catfish (Clarias sp.) Wahyu Afrilasari; . Widanarni; Anja Meryandini
HAYATI Journal of Biosciences Vol. 23 No. 4 (2016): October 2016
Publisher : Bogor Agricultural University, Indonesia

This study aimed to analyze the effect of Bacillus megaterium PTB 1.4 on the population of intestinal microflora, digestive enzyme activity, and the growth of catfish. Gnotobiotic and normal fish were used. Treatment using gnotobiotic was divided into gnoto (with feed and 100 μg/mL rifampicin) and gnotoplus (with feed, 100 μg/mL rifampicin, and 1% probiotic); whereas treatment using normal fish was divided into normalplus (with feed and 1% probiotic) and normal (only feed). The amount of bacteria on gastrointestinal tract was measured 30 days after treatments using the total plate count method. The results indicated no significant difference in bacterial growth between gnotobiotic and normal fish. The total amount of probiotic bacteria with normalplus treatment was significantly different with gnotoplus. The activity of protease and amylase enzymes, and specific growth rate in normalplus treatment were significantly higher (p < 0.05) than other treatments. Bacillus megaterium PTB 1.4 increased the activity of digestive enzymes and the growth of catfish.

Biofungicide Producing Bacteria: an In Vitro Inhibitor of Ganoderma boninense Ade Irma; Anja Meryandini; Bedah Rupaedah
HAYATI Journal of Biosciences Vol. 25 No. 4 (2018): October 2018
Publisher : Bogor Agricultural University, Indonesia

Oil palm is widely known as one of vegetable oil sources and the main comodity in Indonesian agriculture because of the benefits in non-food and food industries. Ganoderma boninense attack results in considerable losses to agriculture. Chemical control creates a harmful effect on health and the environment. Biocontrol is required to take over the function of chemical control. This study aimed to select bacteria that produce bioactive compounds as biofungicide against G. boninense pathogenic fungi and identify bacteria producing biofungicide using molecular method. The stages of bacterial isolate selection were performed through the selected hemolysis and isolate tests in the antagonistic test. Bacteria were extracted using ethyl acetate and their extract activity were tested. Analysis of bioactive compounds was conducted using thin layer chromatography (TLC) and the identification was based on 16S rRNA gene. The result of bacterial pathogenic test was obtained from two selected bacterial isolates namely 11B LB and 11B MD. Both bacterial isolates showed antagonistic effects by forming an inhibitory zone against G. boninense growth with percentage of inhibitor of 81 and 75%. Activity test of bacterial extract showed that crude extract of bacterial isolate 11B MD had the highest inhibitor activity that is 88.34%. TLC analysis proved that the active extract of bacteria containing metabolite compounds had Rf value of 0.1, 0.28, and 0.38. Isolate bacteria 11B MD was identified as Pseudomonas aeruginosa.

Production and Characteristics of Yeast Dextranase from Soil Vestika Iskawati Wahidul Hijah; Titi Candra Sunarti; Anja Meryandini
HAYATI Journal of Biosciences Vol. 26 No. 1 (2019): January 2019
Publisher : Bogor Agricultural University, Indonesia

The existence of dextran in sugar cane juice is a major problem in the sugar industry, causing substantial losses. Treatment of dextran through enzymatic hydrolysis using dextranase is highly recommended as the most suitable method at this time because this is more effective and more economical. This study investigated the production and characterization of dextranase from local isolate yeast to degrade dextran on sugar cane juice. The selected yeast was identified on the basis of molecular identification. Dextranase was produced from the culture with the best carbon and nitrogen sources then was characterized. Application of enzyme was also evaluated. As a selected isolate, F4 had the closest relationship with Pichia kudriavzevii. The highest production of dextranase was induced by the supplementation of glucose and combination of yeast extract and peptone. The enzyme had optimum working condition at pH 7, temperature at 30°C and it is more stable at 4°C of storage temperature. The cation Na+ played key role as co-factor while K+ and Ca2+ were detected as inhibitor of the enzyme. Dextranase from F4 isolate can hydrolyze dextran both in pure and in mixed dextran substrate, but with a lower hydrolysis rate.

Community of Soil Actinobacteria in PTPN VI Oil Palm Plantation Jambi (Sumatra, Indonesia) Based on Amplicon Sequencing of 16S rRNA Gene Mazidah Noer Inayah; Yulin Lestari; Anja Meryandini
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.389-398

In Sumatra, Indonesia, increased oil palm production encourages land expansion for oil palm plantations. And soil Actinobacteria have a potential role in agriculture and plantations ecosystems. The use of fertilizer and herbicide affects soil microbial diversity, including Actinobacteria. This research analyzed and investigated the community composition and diversity of Actinobacteria in soils of oil palm plantations in Jambi Sumatra. Amplicon-based analysis of the 16S rRNA gene (V3-V4 hypervariable region) was used to amplify actinobacterial full-length 16S sequences. The V3-V4 actinobacterial specific 16S rRNA gene sequencing was done using Next-Generation Sequencing. This study confirmed that actinobacterial specific 16S rRNA gene primer could amplify the actinobacterial 16S rRNA gene. Frankiales dominated the community composition of soilborne Actinobacteria. The diversity and community composition of soilborne Actinobacteria were not significantly affected by the interaction between fertilization and weed treatments. Furthermore, the use of NPK fertilizer significantly affected the abundance of Kineosporiales, whose abundance increased with the increasing concentration of NPK fertilizer. The interaction between fertilization and weeding treatments in the oil palm plantations has no impact on soil Actinobacteria's community composition and diversity.

Ethanol Productivity of Ethanol-Tolerant Mutant Strain Pichia kudriavzevii R-T3 in Monoculture and Co-culture Fermentation with Saccharomyces cerevisiae Nurfadhilla Rahmadhani; Rika Indri Astuti; Anja Meryandini
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.435-444

We previously developed ethanol-tolerant P. kudriavzevii R-T3 (P.ku R-T3) mutant from its parental strain P.ku R-WT by evolutionary adaptation method. Hence, we further analyze the ethanol productivity of the particular isolates in a monoculture and co-culture with industrial yeast Saccharomyces cerevisiae BY4741 at various inoculum ratio. Based on the spot assay, R3 mutant yeast showed better cell viability under 10% ethanol stress than the wild type, potentially due to the high expression level of PKINO1 gene involved in the synthesis of inositol. In the monoculture fermentation, S. cerevisiae could use glucose, while P.ku could use mixed glucose and xylose as carbon sources for ethanol fermentation. P.ku R-T3 performed the most potential ethanol kinetics parameters, including the highest ethanol production (10.10 g/L), ethanol productivity (0.21 g/L/h), and fermentation efficiency (84.36%). Upscaling the inoculum of P.ku R-T3 by ten times resulted in 10% higher ethanol production. However, the highest substrate utilization rate did not indicate an increase in ethanol production. Indeed, P.ku R-T3 showed a low mixed substrate use but produced higher ethanol production than S. cerevisiae, as much as 21-31%, depending on the initial inoculum. Interestingly, the co-culture of P.ku R-T3 and BY4741 did not substantially produce higher ethanol production than the monoculture technique. About 30% reduction of ethanol production was found by co-culturing BY4741 with P.ku R-T3 than P.ku R-T3 alone. Taken together, the monoculture fermentation of P.ku R-T3 remains the promising fermentation technique than that of the co-culture with industrial yeast S. cerevisiae.

Co-Authors . Hamim . KURRATAA’YUN . YOPI . YOPI ., Yopi ., Yopi A, Gading Wilda A, Gading Wilda Abd. Rasyid Syamsuri Abdjad Asih Nawangsih Adawiah, Adilah Ade Andriani ADE ANDRIANI Ade Irma Ahmad Thontowi Aisyah, Nadira Alfred Michael ALINA AKHDIYA Amor Tresna Karyawati, Amor Tresna Andika Susantri Anggreandari, Rizky Antonius Suwanto Apon Zaenal Mustopa Ardana Kurniaji Ariandi Arina Amalia Putri Aris Tri Wahyudi Ariyanto, Yogy Satria Armita, Dea Asrianti Basri Atit Kanti Azizah Hikma Safitri Azizah, Malikah Bambang Prasetya Basri, Asrianti Basri, Asrianti Bedah Rupaedah Berutu, Cocok Ana Maryani Berutu, Cocok Ana Maryani Besty Maranatha Birahy, Deford Cristy Candra Sunarti , Titi Cocok Ana Maryani Berutu Cynthia Rizka Riani DAROJATUL ULYA Deden Saprudin DERI YURATMOKO DEWI APRI ASTUTI Dewi, Fitria DIMAS ANDRIANTO DINI NURDIANI Dwi Ambarawati Dyah Iswantini EKA RURIANI Elly Rosyidah Engelhaupt, Martin Esti Utarti Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Fathiah, Muhammad Fadhil Fathin Hamida Ferry Mutia Fitria Dewi Glisina Dwinoor Rembulan Hairani, Atikah Hamim Hamim Hamtini - Hamtini Hanni Tsaaqifah Hari Eko Irianto Hartono, Faisal Diniamal Hasrul Satria Hasrul Satria Nur Hasrul Satria, Hasrul I Komang Gede Wiryawan Ifah Munifah Iman Rusmana Inayah, Mazidah Noer IRA ERDIANDINI, IRA It Jamilah LAKSMI AMBARSARI Lenni Fitri Lenni Fitri Lilis Nuraida Lily Nathalia Loli Natalia Lukman, Zulfiqar Maggy T Suhartono Maggy Thenawidjaja Suhartono Maggy Thenawidjaya Suhartono Mahsunah, Anis Herliyati Manguntungi, Baso Maranatha, Besty Mareistia Fassah, Dilla Maria Sugiharti Marini Wijayanti Muhammad Nur Kholis, Muhammad Nur Muhammad Subhan Hamka MUNTI YUHANA MUTIA, FERY NANIK RAHMANI Nanik Rahmani Nanik Rahmani NAOMI, APRILIA Natalia, Loli Nathalia, Lily Niken Financia Gusmawati NISA RACHMANIA MUBARIK Nunuk Widhyastuti NUNUK WIDHYASTUTI Nur Richana Nur, Hasrul Satria Nurfadhilla Rahmadhani Nurhasna, Aprilia Prihandono, Prima Agung Puspitasari, Dian Japany Rahayu Wulan RAMADHAN, ZULFA AULIA Rika Indri Astuti Rizky Anggreandari Rosyidah, Elly SAFITRI NURLAELA SHANTI RATNAKOMALA Sidiq, Muhammad Raffel Sipriyadi Sitti Rahbiah Akram SOFIYANTO, M. EDY Sri Estuningsih Sri Koerniati Sri Listiyowati SUHARSONO Sumarni Nompo Sunarti, Titi Suyono, Meisy Nawang Titi Candra Sunarti TRIO HENDARWIN Turnip, Enita Romasni Umi Fatmawati Vestika Iskawati Wahidul Hijah Wahyu Afrilasari Wahyu Widosari WIDANARNI WIDANARNI Widosari, Wahyu WULAN, RAHAYU Yantyati Widyastuti Yaya Rukayadi Yopi - Yopi Yopi YOPI YOPI Yopi, YULIANA, META YULIN LESTARI Zahrani, Siti Mutia

Title Search

Found 17 Documents Search Journal : HAYATI Journal of Biosciences

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 17 Documents
Search
Journal : HAYATI Journal of Biosciences