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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Hortikultura Indonesia (JHI) Jurnal Ilmu Pertanian Agrikultura Jurnal Ilmu Dasar Jurnal Natur Indonesia Agrovigor: Jurnal Agroekoteknologi Pelita Perkebunan AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia UNEJ e-Proceeding JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agriprima : Journal of Applied Agricultural Sciences Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Pengabdian Magister Pendidikan IPA jurnal Ilmu Pertanian (Agricultural Science) INTEGRITAS : Jurnal Pengabdian International Journal on Food, Agriculture and Natural Resources (IJFANRES) Journal of Community Development JIWAKERTA: Jurnal Ilmiah Wawasan Kuliah Kerja Nyata AJAD : Jurnal Pengabdian kepada Masyarakat Literasi: Jurnal Pengabdian Masyarakat dan Inovasi JURNAL BIOLOGI PAPUA International Applied Science PAPUMA: Journal of Comunnity Services Jurnal Natur Indonesia
Sholeh Avivi
Faculty Of Agriculture University Of Jember
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : UNEJ e-Proceeding

 1 
The Efficiency of GFP Gene Transformation on Peanut Embryo somatic Using Agrobacterium and Particle Bombardment Avivi, Sholeh; Dietzgen, Ralf G.; Higgins, Colleen M.; ., Sudarsono
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

The aims of this research were to (1) compare the effectiveness of established Agro bacterium mediated transformation and bombardment mediated transformation on peanut (2) evaluate the integration of green fluorescent protein (GFP) and hygromycin resistance (hygr) gene on the peanut embryosomatics. To achieve those objectives we shoot 1200 explants using bombardment and transform 1000 explants using Agrobacterium mediated transformation. Both of Agrobacterium mediated transformation and particle bombardment could give positive embryos transformed.  Particle bombardment gave 4.5% transformation efficiency while Agrobacterium mediated transformation gave only 1.8%. From this research, we concluded that particle bombardment more efficient and gave more transgenic explants than Agrobacterium mediated transformation. Keywords: agrobacterium, bombardment, GFP.
MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERS OF CASSAVA (MANIHOT ESCULENTA CRANTZ) WHICH WET TOLERANT Rahmawati Rahmawati; Tri Agus Siswoyo; Didik Puji Restanto; Sri Hartatik; Sigit Soeparjono; Sholeh Avivi
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Weather conditions often unstable changed and increase the floods of agricultural land. Land which was originally drought will experience wetness and need variety of a plant that wetness tolerant. This study were aimed to get cassava that wet tolerant and to study the morphological, physiological characters of cassava grown on wetness land. The experiment was conducted based on factorial random plot design that consisting two factors with five replications. The first factor were four varieties namely: V1 = Daun Ganja; V2 = Sawi Ketan; V3 = Kasesat Beracun; V4 = Gajah. The second factor was wet treatment consisting of 2 the condition that were K0 = as control, media with 100% field capacity; K1 = as wet treatment by watering of media maintain on -10 cm from media surface. The difference between treatments tested with Duncan Multiple Range Test (DMRT) with the probability 5%. Wet treatment started when plant aged was 2 months after planting, during eight weeks. The observations on variables based on morphological and physiological characters. Result showed that every varieties give the different response on wet treatment. Best response on wet tolerant variety shown by variety code of V2 (Sawi Ketan) were indicated by plant height, total leaf number produced, stem diameter, fresh weight of root, dry weight of root, fresh weight of shoot, dry weight of shoot, photosynthesis index, stomata conductivity and root volume.
The Efficiency of GFP Gene Transformation on Peanut Embryo somatic Using Agrobacterium and Particle Bombardment Sholeh Avivi; Ralf G. Dietzgen; Colleen M. Higgins; Sudarsono .
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aims of this research were to (1) compare the effectiveness of established Agro bacterium mediated transformation and bombardment mediated transformation on peanut (2) evaluate the integration of green fluorescent protein (GFP) and hygromycin resistance (hygr) gene on the peanut embryosomatics. To achieve those objectives we shoot 1200 explants using bombardment and transform 1000 explants using Agrobacterium mediated transformation. Both of Agrobacterium mediated transformation and particle bombardment could give positive embryos transformed.  Particle bombardment gave 4.5% transformation efficiency while Agrobacterium mediated transformation gave only 1.8%. From this research, we concluded that particle bombardment more efficient and gave more transgenic explants than Agrobacterium mediated transformation. Keywords: agrobacterium, bombardment, GFP.
Co-Authors - Asmuni Abdul Jalil Abdul jalil Adam Ramadhan Adhitya Wardhono Adi Prawoto Akbar, Muhammad Mizan Ilham Alvan Maulana Azzini Anang Syamsunihar Andri Wahyudi Anggira, Desi Aprila Iga Mufidah Arum, Ayu Puspita Arya Wiranegara Aurelya, Lalyta Azmi Saleh Azzarah Bakhroini Habriantono Bambang Kusmanadhi Bambang Sugiharto Budi Kristanto Cacuk Purnomo Cahyani, Ardhya Pramisti Regita Choirunnisa, Eviyanti Colleen M. Higgins Colleen M. Higgins, Colleen M. dan Muhammad Chozin Dewi Kusuma Wardani Djoko Soejono Dwi Erwin Kusbianto Dyah Ayu Savitri Elsye L. Sisworo Faesol, Nurul Galang Rizki Ramadhan Gatot Subroto HAJRIAL ASWIDINNOOR Haliza, Nurhayadatul Halwiyah, Lailatul Hartita, Margaretha Putri Hoesain, Mohammad Ikrarwati Ikrarwati Imelda, Laela Vita Intansari, Herlindha Angghelinna Devfi Irfa' Yudayantho Kacung Hariyono Ketut Anom Wijaya Kim, Kyung Min KOMARUDIN IDRIS Laksono Trisnantoro Lenny Widjayanthi Maulidina, Niswah Saffanah Moh. Syahrul Munir Mohammad Nur Khozin Mohammad Ubaidillah Muhammad Ghufron Rosyady Rosyady Muhammad Hazmi Nasir, M Abd Nisa Budi Arifiana Novelia, Eka Putri Parawita Dewanti Pio Tifar Ananda Prabowo, Rachmad Udhi Pradika, Yoga Reno Ayom Prayoga, Mohammad Candra Priyanto Andi Prasetyo Puji Rahayu Puspito, Agung Nugroho Qori’ah, Ciplis Gema Raden Soedradjad Rahmawati Rahmawati Ralf G. Dietzgen Ralf G. Dietzgen, Ralf G. Ratih Apri Utami Reny Fauziah Oetami Restanto, Didik Restanto, Didik Pudji Rusdiana, Riza Yuli Satriyas Ilyas Setiyono Setiyono Setiyono Setiyono Sigit Soeparjono Sitompul, Novrida Yanti Slameto Slameto Slameto Slameto Sobah, Naqiyyah Nada Soetilah Hardjo Soedarmo Sri Hartatik Subroto, Gatot Sudarsono Sudarsono . Sudarsono Sudarsono Sugeng Winarso Sugeng, Santoso Suliswanto, Eko Nur Suud, Hasbi Mubarak Tri Agus Siswoyo TRI HANDOYO Tri Widyastuti Ubaidillah, Mohammad Viki Bayu Wibisono Wahju Q. Mugnisjah Wahyu Indra Duwi Fanata Wulanjari, Distiana Yulianti, Annisa Zahrosa, Dimas Bastara Zakariyya, Fakhrusy Zulfa Maulida