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All Journal Meditory : The Journal of Medical Laboratory Jurnal Ilmu Kedokteran dan Kesehatan Jurnal Analis Medika Biosains (JAMBS) JPP Jurnal Kesehatan Poltekkes Palembang Jurnal Pengabdian Masyarakat Sasambo Quality : Jurnal Kesehatan Gemakes: Jurnal Pengabdian Kepada Masyarakat Jurnal Kefarmasian Indonesia Journal of Indonesia Laboratory Students (JILTS)
RAI WIADNYA, IDA BAGUS
POLTEKKES KEMENKES MATARAM
Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Pengaruh Kualitas Tidur Terhadap Kadar Hemoglobin Pada Ibu Hamil Berdasarkan Trimester Kehamilan Di Wilayah Narmada Malia Septiana; Siti Zaetun; Dr. Ershandi Renshaleksmana; Ida Bagus Rai Wiadnya
Journal of Indonesia Laboratory Students (JILTS) Vol. 2 No. 2 (2023): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v2i2.35

Abstract

Latar Belakang: Ketika proses kehamilan keluhan yang muncul berdampak kepada penurunan kualitas tidur dan berimbas kepada penurunan kesehatan akibat dari proses perbaikan sel yang tidak sempurna salah satunya biosintesis sel darah merah yang menyebabkan penurunan kadar hemoglobin dalam darah. Pengukuran kualitas tidur menggunakan instrument PSQI (Pittsburgh Sleep Qualilty Index) dan alat pengukuran kadar hemoglobin menggunakan metode POCT.Tujuan: Mengetahui pengaruh kualitas tidur terhadap kadar hemoglobin pada ibu hamil berdasarkan trimester kehamilan.Metode penelitian: Observasional analitik dengan pendekatan cross sectional dan data yang di peroleh di olah menggunakan uji statistic non parameetrik yaitu mann-whitney test.Hasil penelitian: kualitas tidur bu hamil trimester I, II, dan III 14 (46,7%) buruk dan 16 (53,3%) diantaranya baik. Rerata kadar hemoglobin trimester I 13,13 gr/dl, trimester II 10,14 gr/dl, trimester III 12,01 gr/dl. Hasil uji mann-withnwy test di dapatkan nilai probabilitas p= 0,017 kurang dari 0,05.Kesimpulan:Terdapat pengaruh yang signifikan antara kualitas tidur terhadap kadar hemoglobin pada ibu hamil berdasarkan trimester kehamilan
The Difference In The Results Of Urine Protein Levels In The Semi-Quantitative Method Of Esbach In Urine Accommodates 12 Hours With 24 Hours In Patients With Urinary Tract Infections Septiawan, Dandi; Pauzi, Iswari; Tantontos, Erlin Yustin; Wiadnya, Ida Bagus Rai
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 2 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i2.378

Abstract

Urinary tract infection is a common infection in the community caused by the growth of microorganisms in the human urinary tract, urinary tract infection is caused by bacteria by identifying the presence of protein in the urine Proteinuria (urine protein) is a protein found in urine that under normal circumstances does not get a high concentration in urine. Protein esbach gold method  standard urine storage using urine storage 24 hours while in urine sampling can use urine storage 12 hours but quantitatively and scientifically there is no known difference in urine protein levels using the semi-quantitative esbach method. Determine the difference in protein content results measured by the  semi-quantitative esbach method  between urine samples collected for 12 hours and those collected for 24 hours in patients with urinary tract infections. This type of research is Observational Analytic with a cross sectional approach. The sample was in the form of urine in patients with urinary tract infections, the number of samples used a total sampling of 24 samples, the data taken were semi-quantitative urine protein levels  of the esbach method. The collected data was then processed using SPSS with Mann Whitney test analysis. The average urine protein content of the 12-hour semi-quantitative method  of esbach was 0.18 g / l, the average urine protein content of the 24-hour semi-quantitative method  of esbach was 0.39 g / l, the difference in protein levels  of the esbach method  in urine collected 12 hours and 24 hours was 0.21 g / l, mathematically there was a difference while statistically there was no difference with the value of (p) = 0.748. 12-hour and 24-hour urine levels showed no difference in protein levels of semi-quantitative esbach method  in patients with urinary tract infections.
Efektivitas Cuka Tuak Aren (Arenga pinnata Merr.) sebagai Reagen Alternatif Pemeriksaan Protein Urin HUSNANINGSIH, ASPARIN; Lalu Srigede; Yudha Anggit Jiwantoro; Ida Bagus Rai Wiadnya
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 2 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i2.138

Abstract

Background: Palm sap (Arenga pinnata Merr.) has an acid content that comes from fermentation in the form of alcohol, then turns into vinegar acid due to an increase in acidity by Acetobecter Acetic bacteria. This vinegar acid has the property of being a weak acid. The vinegar acid content in palm oil can be used as a reagent in urine protein examinations. The principle of urine protein examination is that urine protein is denatured by heating and acid addition. Research Objective: Determine the effectiveness of palm wine vinegar (Arenga pinnata Merr.) as an alternative reagent for urine protein examination. Research Method: This study is quasi-experimental and the sample in this study is protein positive urine with incidental sampling technique. The number of treatments was 3 treatments and replicated 9 times. Research Results: The results of urine protein examination using alternative reagents of palm wine vinegar (Arenga pinnata Merr.) with concentrations of 4%, 6% and 8% have similar results with examinations using 6% acetic acid solution as a control. Conclusion: Palm wine vinegar (Arenga pinnata Merr.) with concentrations of 4%, 6% and 8% is effectively used as an alternative reagent for urine protein examination. Keywords: Palm Vinegar, Urine protein, Acetic acid 6%.
Hubungan Kadar Ureum dan Kreatinin Dengan Kadar Hemoglobin (Hb) pada Penderita Diabetes Melitus Tipe 2 Widiasih, Ni Kadek; Wiadnya, Ida Bagus Rai; Manu, Thomas Tandi; Kristinawati, Erna
JPP JURNAL KESEHATAN POLTEKKES PALEMBANG Vol 20 No 1 (2025): JPP (Jurnal Kesehatan Poltekkes Palembang)
Publisher : Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jpp.v20i1.2866

Abstract

Background: High blood sugar levels in people with diabetes mellitus will cause blockages and reduce the blood flow rate, thus decreasing the blood supply to the kidneys. This is indicated by high levels of urea and creatinine. Kidney damage leads to decreased production of the hormone erythropoietin, resulting in low oxygen levels and reduced red blood cell production, which can lead to anaemia.. Methods: This research method uses an analytical observation approach with a cross-sectional design. The sampling technique is non-random purposive sampling, Results: The results of the statistical test using the Spearman test showed a P value of 0.00 > 0.05, meaning that Ho is accepted and Ha is rejected. Conclusion: There is no relationship between urea and creatinine levels and haemoglobin (Hb) levels in people with type 2 diabetes mellitus.
Gambaran Kadar Protein Urine dan Hemoglobin (Hb) pada Penderita Hipertensi di RSUD Patut Patuh Patju Melya Alimatul Sa’adati; Ida Bagus Rai Wiadnya; I Wayan Getas; Pancawati Ariami
Journal of Indonesia Laboratory Students (JILTS) Vol. 4 No. 1 (2025): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v4i1.136

Abstract

Latar Belakang : Hipertensi dapat memicu penyakit lain yang mematikan dan dapat mengakibatkan komplikasi seperti serangan jantung, gagal jantung, stroke, dan gagal ginjal. Tekanan darah yang tinggi memaksa ginjal untuk bekerja lebih keras yang mengakibatkan rusaknya sel ginjal yang ditandai dengan adanya proteinuria. Hemoglobin yang abnormal, baik terlalu rendah maupun terlalu tinggi dapat mempengaruhi tekanan darah melalui mekanisme yang berbeda. Tujuan Penelitian : Mengetahui gambaran protein urine dan hemoglobin (Hb) pada penderita hipertensi di RSUD Patut Patuh Patju. Metode Penelitian: Menggunakan rancangan observasional analitik dengan pendekatan cross-sectional. Teknik pengambilan sampel non-random purposive sampling, dengan analisi data secara deskriftif. Hasil Penelitian : Tekanan darah rata-rata 154.7 mmHg, nilai tertinggi 190 mmHg nilai terendah 140 mmHg, protein urine 7 sampel positif 1 (+) 23.3% dan 2 sampel positif 2 (++) 6.7% sedangkan protein urine negatif (-) 21 sampel 70%, kadar rata-rata hemoglobin 14,2 g/dL, tertinggi 17.7 g/dL, terendah 10.8 g/dL. Kesimpulan: Pada penderita hipertensi protein urine positif 1 (+) 23.3%dan positif 2 (++) 6.7%, kadar hemoglobin tidak terjadinya peningkatan terhadap hipertensi.
Gambaran Hasil Aglutinasi Pemeriksaan Widal Slide Metode Semi Kuantitatif pada Pasien Demam Tifoid di Puskesmas Kediri Khairunnisa; Yunan Jiwintarum; Ida Bagus Rai Wiadnya; Erlin Yustin Tatontos
Journal of Indonesia Laboratory Students (JILTS) Vol. 4 No. 1 (2025): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v4i1.184

Abstract

Latar Belakang : Pemeriksaan Widal slide telah menjadi salah satu metode diagnostik yang umum digunakan untuk mendeteksi infeksi demam tifoid. Pada pemeriksaan Widal slide metode kualitatif, volume sampel yang digunakan tidak terukur sehingga penentuan hasil titer ditentukan dengan melihat banyak atau sedikitnya aglutinasi yang terjadi. Hal ini dapat menyebabkan hasil positif palsu terutama karena variasi interpretasi dan faktor-faktor lain yang dapat mempengaruhi hasil. Dalam penentuan hasil titer Widal slide perlu melakukan pengukuran volume sampel dan melakukan validasi hasil dengan pengenceran serum atau plasma menggunakan metode semi kuantitatif, sehingga pembacaan hasil titer dapat dilakukan dengan melihat aglutinasi terakhir yang terbentuk. Tujuan Penelitian : Mengetahui gambaran hasil aglutinasi pemeriksaan Widal slide metode semi kuantitatif pada pasien demam tifoid di Puskesmas Kediri. Metode Penelitian : Penelitian ini merupakan penelitian deskriptif analitik dengan populasi sampel penelitian adalah pasien demam tifoid di Puskesmas Kediri. Sebanyak 20 sampel penelitian diambil dengan teknik accidental sampling. Pengumpulan data diperoleh dengan pemeriksaan Widal slide pada pasien demam tifoid dan didapatkan gambaran hasil aglutinasi pada pemeriksaan Widal slide metode kualitatif dan metode semi kuantitatif. Hasil Penelitian : Didapatkan gambaran hasil aglutinasi pemeriksaan Widal slide metode semi kuantitatif dengan titer 1/80 yang menunjukkan aglutinasi terakhir pada sampel 20 µl, titer 1/160 menunjukkan aglutinasi terakhir pada sampel 10 µl, titer 1/320 menunjukkan aglutinasi terakhir pada sampel 5 µl, dan titer 1/640 menunjukkan aglutinasi terakhir pada sampel 2,5 µl. Kesimpulan : Gambaran hasil aglutinasi Widal slide metode semi kuantitatif dapat memberikan hasil titer lebih tepat daripada menggunakan metode kualitatif.
Perbedaan Kadar Hemoglobin Sebelum dan Setelah Mengonsumsi Minuman Tradisional Pakombo Pada Masyarakat Penderita Anemia di Wilayah Kecamatan Belo Kabupaten Bima Eka Atyi Purwasih; Ida Bagus Rai Wiadnya; Thomas Tandi Manu; I Wayan Getas
Journal of Indonesia Laboratory Students (JILTS) Vol. 4 No. 1 (2025): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v4i1.250

Abstract

Background: Anemia is a condition characterized by a decrease in hemoglobin levels, which can lead to fatigue, weakness, and reduced work productivity. Pakombo is a traditional drink from Bima, believed to help increase hemoglobin levels due to its natural ingredients rich in iron and other essential nutrients. Objective: To determine the difference in hemoglobin levels before and after consuming Pakombo in individuals with anemia in Belo Subdistrict, Bima Regency. Method: This study employed an observational analytic design. The sample consisted of 31 individuals with anemia, selected using a non-random sampling technique. Hemoglobin level measurements were analyzed using the paired t-test. Results: The average hemoglobin level before consuming the traditional beverage Pakombo was 8.74 g/dL, and after consumption, it increased to 10.20 g/dL. The statistical analysis using the paired t-test showed a p-value of 0,000 < (α) 0,05. Conclusion: There is a significant difference in hemoglobin levels before and after consuming the traditional Pakombo drink among individuals with anemia in Belo Subdistrict, Bima Regency.
Cross Test of ICT-TB and Genexpert Examination in Suspected Cases of M. tuberculosis Infection at Bima Regency Regional Hospital Wiadnya, Ida Bagus Rai; Sani, Syahrul; Srigede, Lalu
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 1 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i1.426

Abstract

GeneXpert is able to detect specimens that are positive for M. tuberculosis in a shorter time and with high sensitivity and specificity. ICT-TB is used to detect the presence of antibodies produced by M. tuberculosis infection in the body. This response requires time to form. However, actually ICT-TB is an easier method compared to Genexpert. So this research was carried out to compare the results of the two methods. Research Objective: To determine the results of cross-testing of ICT-TB and Genexpert examinations on cases of suspected M. tubeculosis infection at the Bima Regency Hospital. Research Method: This is an analytical observational study with a cross-sectional design. The samples used in this research were 52 samples and the data were analyzed descriptively. Research Results: The distribution of suspected tuberculosis based on gender is 54% male and 46% female. Based on age group, Toddlers 13.5%, Children 11.5%, Teenagers 9.6%, Adults 44.2% and Elderly 21.2%. ICT-TB examination results are negative. Examination with Genexpert on the same patient was negative. Conclusion: From the results of research using ICT-TB and GeneXpert in patients with suspected M. tuberculosis infection, negative results were obtained.
The Effect of Sambang Darah Leaf Filtrate (Excoecaria Cochinchinensis L) to Reduce The Bleeding Time in Skin Wounds of White Rats (Rattus Norvegicus) Hijriani, Baiq Isti; Zaetun, Siti; Wiadnya, Ida Bagus Rai
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.298

Abstract

Hemostasis is the process of stopping bleeding from a damaged blood vessel. When bleeding occurs, the body will naturally respond with a hemostatic mechanism to stop the bleeding. Indonesian people have long used plants in medicine, one of which is sambang darah (Excoecaria cochinchinensis L) plant. Sambang darah has a chemical compound that is thought to stop bleeding. The purpose of this study was to determine the potential of sambang darah filtrate to reduce the bleeding time in skin wounds of white rats (Rattus norvegicus). This study is a pre-experimental study with a static group comparison approach. The number of experimental units in this study were 10 white rats which were divided into treatment group and control group. Data analysis used the Independent Sample T Test. The results showed that the average of bleeding time in the skin wounds of white rats without drops of sambang darah leaf filtrate was 252 seconds. While the average of bleeding time in the skin wounds of white rats with drops of sambang darah leaf filtrate is 163 seconds. The conclusion of this study is sambang darah (Excoecaria cochinchinensis L) leaf filtrate have a potential to reduce the bleeding time in the skin wounds of white rats (Rattus norvegicus).  
Analisis Konsentrasi Letal Dan Waktu Letal Isolat Jamur Aspergillus Niger Terhadap Nyamuk Aedes Aegypti Kadek Sinta Cahyani, Ni Diah; Wiadnya, Ida Bagus Rai; Khusuma, Ari; Getas, I Wayan
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 2 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i2.264

Abstract

Latar Belakang: Nyamuk Aedes aegypti merupakan penyebab utama penyakit DBD, kasus DBD di dunia pada tahun 2015 sebanyak 3,2 juta kasus. Di Indonesia pada tahun 2018 terdapat 65.602 kasus. Upaya pengendalian terhadap vektor DBD telah banyak dilakukan baik dengan menggunakan insektisida kimia dan alami salah satunya dengan jamur Aspergillus niger sebagai fungi entomopatogen. Tujuan Penelitian: Mengetahui konsentrasi letal dan waktu letal dari isolat jamur Aspergillus niger terhadap nyamuk Aedes aegypti.  Metode Penelitian: Penelitian ini merupakan penelitian true eksperimental dengan rancangan acak lengkap. Perlakuan dengan 3 konsentrasi isolat jamur Aspergillus niger yaitu 10-5, 10-6, 10-7 spora/ml dengan 6 replikasi. Penelitian ini menggunakan obat nyamuk semprot sebagai kontrol positif. Data diperoleh dianalisa secara statistik menggunakan analisis probit. Hasil Penelitian: Konsentrasi isolat jamur Aspergillus niger yang efektif terhadap nyamuk Aedes aegypti adalah konsentrasi 10-7. Hasil analisis probit uji patogenitas isolat jamur Aspergillus niger terhadap nyamuk Aedes aegypti dengan tingkat kepercayaan 95% didapatkan LC50, LC70, LC90 adalah 6,1 x 107 spora/ml, 8,5 x 107 spora/ml, 1,1 x 108 spora/ml dan diketahui LT50, LT70, LT90 berturut-turut adalah 1,919 jam, 1,510 jam, 1,184 jam. Kesimpulan: Konsentrasi isolat jamur Aspergillus niger secara signifikan memberikan pengaruh terhadap kematian nyamuk Aedes aegypti.
Co-Authors Agrijanti, Agrijanti Ananda, Dewa Ayu Putu Wiweka BaiqIsti Hijriani Dewi, Lale Budi Kusuma Dr. Ershandi Renshaleksmana Dwi Kurnia Febrianti kurnia Eka Atyi Purwasih Erlin Yustin Tatontos Erna Kristinawati Ersandhi Resnhaleksmana Fihiruddin, Fihiruddin Getas, I Wayan Helenia Ananda Feriska Felanis Hijjati Sukmana Gading HUSNANINGSIH, ASPARIN I Gusti Ayu Nyoman Danuyanti I Gusti Ayu Ratih Yuliartini Ayu I Made Suardika I Made Wahyu Waisnawa I Wayan Getas I wayan Getas I Wayan Getas I Wayan Getas Iswari Pauzi Iswari Pauzi Kadek Sinta Cahyani, Ni Diah Khairunnisa Khusuma, Ari Kristinawati, Erna Lale Budi Lalu Sri Gede Lalu Srigede Lalu Srigede, Lalu Lidia Febrianisa Malia Septiana Maruni Wiwin Diarti Melya Alimatul Sa’adati Minovriyanti, Eka Muhammad Luthfi Ni Diah Kadek Sinta Cahyani Ni Kadek Defyantari Ni Luh Gita Dewi Lestari Ni Luh Putu Pebrita Ni Luh Putu Pebrita Anjar Santi Pancawati Ariami Pauzi, Iswari Pebrita Anjar Santi, Ni Luh Putu Pebrita Anjar Santi, Ni Luh Putu Rohmi Rohmi, Rohmi Salsabila, Yaumi Tasu’a Sani, Syahrul Septiawan, Dandi Siti Zaetun Siti zaetun Siti zaetun Siti Zaetun, Siti Sri Hartuti Suaryana, I Made Chandra Thomas Tandi Manu Urip Urip Urip Urip, Urip Waisnawa, I Made Wahyu Wayan Getas Widiasih, Ni Kadek Yaumi Tasu’a Salsabila Yudha Anggit Yudha Anggit Jiwantoro Yunan Jiwintarum Zaenal Fikri Zainal Fikri Zainal Fikri Zainal Fikri, Zainal Ziana Dia Ningsih