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Aktivitas Antibakteri Ekstrak Akar Binjai (Mangifera caesia Jack.) terhadap Shigella dysenteriae dan Salmonella typhi In Vitro Galuh Eka Suryani; Agung Biworo; Lia Yulia Budiarti
Homeostasis Vol 2, No 1 (2019)
Publisher : Homeostasis

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Abstract: Binjai is plant that’s widely spread in South Kalimantan. Binjai’s root contains saponins and tannins as antibacterial. The purpose of study to analyze the differences in inhibitory activity of binjai’s root ethanol extract against Shigella dysenteriae and Salmonella typhi in vitro. This study used true experimental studies that’s posttest with control group design, consisting of 10 treatments of binjai’s root ethanol extract (50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%) and ciprofloxacin (positive control). Data analysis was performed by one way ANOVA, post hoc Duncan, and t independent test (α = 0.05). The results of study showed that there were significant differences inhibitory power and inhibitory power increased with increasing concentration also at the same concentration, the inhibitory activity of binjai’s root ethanol extract was greater against to Salmonella typhi (23.12 mm) than Shigella dysenteriae (20.81 mm). Ethanol extract of binjai’s root has optimum inhibition at concentration 90% towards the growth both of bacteria. The conclusion is there are differences inhibitory activity of binjai’s root ethanol extract on the growth of Shigella dysenteriae and Salmonella typhi in vitro. Keywords: antibacterial activity, binjai’s root extract, Shigella dysenteriae, Salmonella typhi, in vitro. Abstrak Binjai merupakan tanaman yang banyak tersebar di Kalimantan Selatan. Akar binjai mengandung saponin dan tanin sebagai antibakteri. Tujuan penelitian ini adalah menganalisis perbedaan aktivitas daya hambat ekstrak etanol akar binjai terhadap Shigella dysenteriae dan Salmonella typhi secara in vitro. Penelitian ini menggunakan studi true experimental dengan rancangan posttest with control group design, terdiri dari 10 perlakuan ekstrak etanol akar binjai (50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%) dan siprofloksasin (kontrol positif). Analisis data dilakukan dengan uji one way ANOVA, post hoc Duncan, dan t independent  (α=0,05). Hasil penelitian menunjukkan terdapat perbedaan daya hambat yang bermakna dari masing-masing perlakuan dan daya hambat meningkat seiring dengan peningkatan konsentrasi serta didapatkan pada konsentrasi yang sama, aktivitas daya hambat ekstrak etanol akar binjai lebih besar terhadap Salmonella typhi (23,12 mm) dibandingkan Shigella dysenteriae (20,81 mm). Ekstrak etanol akar binjai memiliki daya hambat optimum pada konsentrasi 90% terhadap pertumbuhan kedua bakteri uji. Simpulannya adalah terdapat perbedaan aktivitas daya hambat ekstrak etanol akar binjai terhadap pertumbuhan Shigella dysenteriae dan Salmonella typhi secara in vitro. Kata-kata kunci: aktivitas antibakteri, ekstrak akar binjai, Shigella dysenteriae, Salmonella typhi, in vitro.
Perbandingan Potensi Antibakteri Infus Akar dari Tanaman Akar Kuning (Fibraurea tinctoria Lour.) terhadap Pseudomonas aeruginosa dan Escherichia coli Sonya Esti Kholifa; Lia Yulia Budiarti; Mohammad Bakhriansyah
Homeostasis Vol 4, No 1 (2021)
Publisher : Homeostasis

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Abstract: Fibraurea tinctoria Lour. plant is commonly used by Dayak tribes in Central Borneo as a herbal medicine. Previous studies showed that this plant contains alkaloids, flavonoids, saponins, terpenoids, tanins as antibacterial compounds. This study was aimed to analyze the comparison of antibacterial potency of Fibraurea tinctoria Lour. against Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E.coli). This research method was a true-experimental study with a post-test only with the control group design. Fibraurea tinctoria Lour. concentration were 15%, 35%, 50%, 75%, 100%. Ciprofloxacin 5 μg as a positive control and distilled water as a negative control. Parameters that measured in this study were the inhibitory zone diameter (milimeter) of bacterial growth. The data was analyzed using the One-Way ANOVA test and LSD Post-hoc test at the 95% confidence level. The results of this research showed that all concentration had antibacterial effect against P.aeruginosa and E.coli. In P.aeruginosa, the smallest inhibitory zone was 8.9 mm (35%) and E.coli 7.99 mm (15%); while the largest inhibitory zone (100%) was 18.62 mm in P. aeruginosa and 19.31 mm in E.coli. The conclusion is the antibacterial activity of Fibraurea tinctoria Lour. against E.coli was better than against P.aeruginosa. Keywords: antibacterial activity, yellow root infusion, Pseudomonas aeruginosa,  Escherichia coli. Abstrak:  Tanaman akar kuning (Fibraurea tinctoria Lour.) merupakan tanaman yang dimanfaatkan masyarakat Dayak di Kalimantan Tengah sebagai obat herbal alami. Penelitian terdahulu menunjukkan tanaman ini memiliki senyawa metabolit sekunder yang berperan sebagai antibakteri seperti flavonoid, alkaloid, saponin, terpenoid, dan juga tanin. Penelitian ini bertujuan untuk membandingkan potensi antibakteri infus akar kuning terhadap bakteri Pseudomonas aeruginosa (P.aeruginosa) dan bakteri Escherichia coli (E.coli). Metode yang digunakan true-experimental dengan post-test  with control group design. Perlakuan yang digunakan ialah sediaan infus konsentrasi (15%, 35%, 50%, 75%, 100%), siprofloksasin 5 μg, dan akuades masing-masing sebagai kontrol positif dan negatif. Parameter yang diukur pada penelitian ini ialah diameter zona hambat (milimeter) pertumbuhan bakteri. Data akan dianalisis menggunakan uji statistik One-Way ANOVA dan uji lanjutan Post-hoc LSD pada tingkat kepercayaan 95%. Hasil penelitian menunjukkan semua perlakuan memberikan efek antibakteri terhadap bakteri uji. Pada P.aeruginosa zona hambat terkecil 8,9 mm (35%) dan E.coli 7,99 mm (15%). Zona hambat terbesar (100%) yaitu 18,62 mm pada P.aeruginosa dan 19,31 mm pada E.coli. Kesimpulan penelitian ini adalah efek antibakteri infus akar kuning terhadap E.coli lebih baik dibandingkan terhadap P.aeruginosa. Kata-kata kunci: antibakteri, infus akar kuning, Pseudomonas aeruginosa, Escherichia coli.
Gambaran Jenis Bakteri pada Tangan Siswa Sekolah Dasar di Sekitar Bantaran Sungai Lulut Banjarmasin Pauline Surya Kurniati; Farida Heriyani; Lia Yulia Budiarti
Homeostasis Vol 2, No 1 (2019)
Publisher : Homeostasis

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Abstract

Abstract: The presence of bacteria on the hands could cause the primary illness towards the body for example the infection of digestive system which proceeds to diarrhea, upper respiratory tract infection and skin diseases. Bacteria on the hands could differ from the location conducted. Therefore, this research is designed in order to obtain the hand’s bacteria of elementary students of the riverbank  Lulut Banjarmasin. The method that employed in this research was observational description alongside the cross sectional. The samples employed on this research were observed through 30 elementary students for each different groups, whom lived within less than 10 m from the riverbank and more than 10 m outside the riverbank. The result of this experiement through the swab hands towards the group of elementary students who lived near the riverbank showed that there were 70% of  Staphylococcus aureus and 30% of Escherichia coli. Whereas, the other group of elementary students who lived outside the area got 66.7% of Staphylococcus aureus and 33.3% of Escherichia coli. Through the analysis, it is indicated that Staphylococcus aureus was the most bacteria established on elementary hands students near the riverbank of Lulut River Banjarmasin. Keywords: Bacteria on hands, the riverbank, outside the riverbank, Lulut River Banjarmasin, elementary student Abstrak: Keberadaan bakteri pada tangan dapat menjadi perantara infeksi saluran cerna seperti diare, infeksi saluran pernapasan akut (ISPA) dan penyakit pada kulit. Jenis bakteri pada tangan dapat berbeda sesuai lokasi daerah. Penelitian ini bertujuan untuk mengetahui gambaran jenis bakteri pada tangan siswa Sekolah Dasar Negeri (SDN) di bantaran Sungai Lulut Banjarmasin. Metode penelitian ini adalah observasional  deskriftif dengan pendekatan cross sectional. Sampel yang digunakan dalam penelitian ini adalah masing-masing sejumlah 30 siswa siswa yang bertempat tinggal di bantaran dengan jarak ≤10 m dari sungai dan diluar bantaran dengan jarak ˃10 m dari sungai. Hasil penelitian pada swab tangan kelompok siswa sekolah dasar (SD) yang bertempat tinggal di bantaran sungai didapatkan Staphylococcus aureus sebanyak  70% dan Escherichia coli sebanyak 30%, sedangkan pada kelompok siswa SD yang bertempat tinggal di luar bantaran sungai didapatkan Staphylococcus aureus sebanyak  66,7% dan Escherichia coli sebanyak 33,3%. Dapat disimpulkan bahwa Staphylococcus aureus merupakan bakteri terbanyak pada tangan siswa SD disekitar bantaran Sungai Lulut Banjarmasin. Kata-kata kunci: Bakteri tangan, bantaran sungai, luar bantaran sungai, Sungai Lulut Banjarmasin, siswa sekolah dasar
Aktivitas Infus Eichornia crassipes Solms. (Eceng Gondok) terhadap Jumlah Koloni Staphylococcus aureus dan Escherichia coli Muhammad Bayu Fernanda; Siti Kaidah; Lia Yulia Budiarti
Homeostasis Vol 4, No 2 (2021)
Publisher : Homeostasis

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Abstract: The water hyacinth plant (Eichornia crassipes Solms.) is abundant aquatic plant that contain antibacterial compounds such as flavonoids and saponin. Antibacterial compounds can act as antiseptic. This study was aimed to analize infusion activities of water hyacinth in reducing the number of bacteriall colonies of Staphylococcus aureus and Escherichia coli. The study was a true experimental with pre and posttest with control group design,used  the water hyacinth infusion ekstract (20%, 40%, 60%, 80%, dan 100%) and alcohol 70%. Data was analyzed with one-way ANOVA and post hoc Duncan (α0.05). The result showed there was a decrease in  number of S.aureus and E.coli colonies after water hyacinth infusion; corresponds to increased concentration. The effect of water hyacinth infusion on S.aureus is larger than E.coli. The conclusion that water hyacibth infusion has activity to reduce the number of colonies of S.aureus and E.coli; effectiveness against S.aureus is greater than againt E.coli. Keywords: Eichornia crassipes Solms., number of bacteria colonies, Staphylococcus aureus, Escherichia coli, reculture isolates, hand swab.  Abstrak: Tanaman Eichornia crassipes Solms (eceng gondok) merupakan tanaman gulma air yang mengandung senyawa antibakteri flavonoid dan saponin, serta berpotensi dimanfaatkan sebagai antiseptik alternatif. Penelitian ini bertujuan menganalisis aktivitas infus eceng gondok dalam menurunkan jumlah koloni Staphylococcus aureus dan Escherichia coli, isolat bakteri uji hasil rekultur swab tangan. Rancangan penelitian ini menggunakan true experimental, pre and posttest with control group design, dengan perlakuan ekstrak infus eceng gondok 20%, 40%, 60%, 80%, dan 100%, serta alkohol 70 %. Analisis data menggunakan uji one-way ANOVA dan post-hoc Duncan (α.0.05). Hasil penelitian didapatkan penurunan jumlah koloni S.aureus dan E.coli sesudah pemberian infus eceng gondok; peningkatan konsentrasi berefek terhadap penurunan jumlah koloni bakteri uji. Efek infus eceng gondok terhadap S.aureus lebih besar dibandingkan terhadap E.coli. Simpulan, infus eceng gondok memiliki aktivitas menurunkan jumlah koloni S.aureus dan E.coli; efektivitas terhadap S.aurues lebih besar dibandingkan terhadap E.coli. Kata-kata kunci: Eichornia crassipes Solms, jumlah koloni bakteri, Staphylococcus aureus, Escherichia coli, isolat rekultur, swab tangan.
The Antibacterial Activity of Infusion of Averrhoa bilimbi L Fruits and Cananga odorata Flowers against Frequently Pathogenic Bacteria Lia Yulia Budiarti; Erida Wydiamala; Najiya Ulfa
Journal of Agromedicine and Medical Sciences Vol 8 No 3 (2022)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v8i3.29312

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Infectious diseases due to opportunistic bacteria and pathogens are still a health problem. Transmission can be prevented by using an alcohol-based antiseptic liquid or naturally. Averrhoa bilimbi L. fruit plants and Cananga odorata flowers contain bioactive compounds that can inhibit bacterial growth. This study aimed to analyze the infusion activity of Averrhoa bilimbi fruit and Cananga odorata flower in single and combined dosage forms against several frequently pathogenic bacteria in vitro. This posttest-only experimental study with a control group design used the paper disc diffusion method. Observation parameters were the diameter of the inhibition zone on the test bacteria after infusion treatment of 50%, 75%, and 100% A.bilimbi fruit and 100% C.odora flowers, as well as 70% alcohol. The results showed that the diameter of the inhibition zone in the combination treatment of A.bilimbi and C.odorata infusion was significantly different from that of the single preparation (p<0.05). The combination of 100% A.bilimbi and 100% C.odorata (ratio 1:1) produced the greatest effect and was equivalent to 70% alcohol. The average inhibition of the combination infusion of Staphylococcus aureus (17.36mm) and Staphylococcus epidermidis (17.57mm) was greater than that of Escherichia coli (14.48 mm) and Salmonella enterica serovar Typhi (14.12mm). In conclusion, the infusion of Averrhoa bilimbi fruit and the Cananga odorata flower had antibacterial activity, and the combined preparation produced a better inhibitory effect than the single preparation. Keywords: antibacterial activity, Averrhoa bilimbi L., Cananga odorata, infusion, single preparation, combination preparation
Antibacterial Activity of Mixed Infusion of Averrhoa bilimbi L. Fruit and Cananga odorata Flowers Against Several Numbers of Bacterial Colonies in Vitro Test Lia Yulia Budiarti; Siti Kaidah; Nur Adnia; Vania Puspitasari Sangadi
Berkala Kedokteran Vol 19, No 1 (2023)
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jbk.v19i1.15705

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Abstract: Antiseptic alcohol 70% is known to be effective in inhibiting the growth of bacterial colonization on the hands, but its use in the long term can cause skin disorders. An alternative antiseptic can be made from a mixture of star fruit (Averrhoa bilimbi L.) and flower Cananga (Cananga odorata) which contains several antibacterial compounds. The purpose of this study was to analyze the antibacterial activity of the infusion mixture of Averrhoa bilimbi and Cananga odorata flowers in inhibiting the growth of Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Salmonella typhi, and Shigella sonnei bacteria. This in vitro research design used a posttest only with control group design with the observation parameter being the number of test bacteria colonies that grew on the observation medium. The treatments tested were a mixture of 50%, 75%, and 100% Averrhoa bilimbi infusion and 100% Cananga odorata, and 70% alcohol control. The results showed that the infusion mixture of Averrhoa bilimbi and Cananga odorata had different inhibitory effects on Gram-positive and Gram-negative bacteria; the number of colonies of Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli was not significantly different from the mixed treatment concentration of 100% (1:1) with 70% alcohol treatment. In conclusion, the infusion mixture of Averrhoa bilimbi fruit and Cananga odorata flower had antibacterial activity against Gram-positive and Gram-negative bacteria, and the best inhibitory effect was obtained in the 100% infusion mixture treatment.
Antibacterial and Wound Healing Activity of Papuyu Fish (Anabas Testudineus) Mucus Erwin Rosadi; Lia Yulia Budiarti; Nor Admi Zayanti; Isnaini Isnaini
Journal of Wetlands Environmental Management Vol 10, No 1 (2022): January-June, 2022
Publisher : Center for Journal Management and Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.94 KB) | DOI: 10.20527/jwem.v10i1.275

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One of the local fish resources in South Kalimantan is Anabas testudineus fish. It is known that A. testudineus fish mucus has antibacterial activity, but this study only looked at the inhibitory of fish mucus. Until now, there has been no test for the Minimum Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) of A. testudineus fish mucus. One of the bacteria that infect wounds is S. aureus. MIC and MBC obtained are used as a reference in determining the concentration of A. testedineus fish mucus gel for wound healing activity test. This study aimed to determine the MIC and MBC of A. testudineus fish mucus in S. aureus and analyze the wound healing activity of A. testudineus fish mucus. MIC and MBC using turbidity test. In this research, 3 fish mucus gel formulas are made. Wound healing activity test on Wistar rats that were given wound initiation with a diameter of 1.5 cm, and were observed after 7 days of treatment. Observations were made covering the area of the wound using image J and histology of rat skin. The results showed that MIC and MBC of A. testudineus fish mucus were 21.875%. Fish mucus gel formula was made into concentrations of 10%, 20% and 40%. The results of the wound healing test showed that giving A. testudineus fish mucus caused a decrease in the area of the wound but based on histology of the skin, the inflammation still occurred.
NILAI KOEFISIEN FENOL DAN JUMLAH KOLONI BAKTERI Escherichia coli PADA PERLAKUAN EKSTRAK KOMBINASI KULIT BUAH Citrus aurantifolia Swingle DAN Citrus hystrix DC Ghina Salsabila; Lia Yulia Budiarti; Husnul Khatimah
Homeostasis Vol 6, No 1 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/ht.v6i1.8812

Abstract

Citrus aurantifolia Swingle (C.aurantifolia) dan Citrus hystrix DC (C.hystrix) mengandung berbagai senyawa antibakteri yang berpotensi sebagai  antiseptik alternatif. Efektivitas antiseptik yang baik mempunyai nilai koefisien fenol (KF) ≥1. Penelitian ini bertujuan menganalisis aktivitas antibakteri ekstrak kombinasi kulit buah C.aurantifolia dan C.hystrix (Ca+Ch) terhadap Escherichia coli  in vitro, berdasarkan parameter nilai koefisien fenol dan jumlah koloni bakteri. Rancangan penelitian posttest-only with control group design ini menggunakan metode uji dilusi untuk uji perlakuan kombinasi ekstrak Ca+Ch dan kontrol dalam menghambat pertumbuhan E.coli.  Hasil uji koefisien fenol, diperoleh rerata nilai KF=1,0667 pada kombinasi ekstrak kulit buah Ca+Ch dan KF=1,0337 pada alkohol 70%; nilai KF ini setara dengan fenol5%. Hasil uji One Way-Anova,  perlakuan ekstrak kombinasi kulit buah (Ca+Ch) pada variasi kombinasi 25% 50%, 75%, 100% (ratio 1:1) terhadap jumlah koloni E.coli, didapatkan rerata jumlah koloni E.coli berbeda bermakna pada perlakuan Ca100%+Ch100% dengan perlakuan lainnya (p> 0,05). Berdasarkan analisi pos-hoc Duncan, jumlah koloni E.coli dari perlakuan Ca75%+Ch100%=35,0 cfu/ml tidak berbeda bermakna dengan antiseptik alcohol 70% (34,0 cfu/ml); ekstrak kombinasi Ca100%+Ch100% menghasilkan efektivitas terbaik terhadap jumlah koloni E.coli (32,0 cfu/ml).  Simpulan penelitian, ekstrak kombinasi kulit buah C.aurantifolia dan C.hystrix memiliki aktivitas antibakteri terhadap E.coli dan miliki efktivitas sebagai antiseptik.
UJI DAYA ANTIBAKTERI EKSTRAK ETANOL DAUN Xylocarpus granatum TERHADAP Staphylococcus aureus ATCC 25923 DAN Streptococcus pyogenes ATCC 12344 Raymona Dewi Ginarti; Agung Biworo; Lia Yulia Budiarti
Homeostasis Vol 6, No 2 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/ht.v6i2.10003

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Xylocarpus granatum (X. granatum) merupakan jenis dari tanaman mangrove yang banyak ditemukan di kawasan hutan mangrove Pulau Burung Kalimantan Selatan. Daun X. granatum diketahui mengandung metabolit sekunder berperan sebagai antibakteri. Tujuan penelitian ini adalah menguji daya antibakteri ekstrak etanol daun (ED) X. granatum terhadap Staphylococcus aureus (S. aureus) ATCC 25923 dan Streptococcus pyogenes (S. pyogenes) ATCC 12344. Rancangan penelitian eksperimental ini menggunakan posttest-only with control group design dengan uji daya hambat menggunakan metode difusi kertas cakram. Kelompok perlakuan uji yaitu ED X. granatum 25%, 50%, 75%, dan 100% serta kontrol kloramfenikol dan DMSO 1%. Diameter zona hambat dari perlakuan ED terhadap S. aureus (9,20 mm–16,36 mm) dan terhadap S. pyogenes (8,13 mm–15,69 mm) zona hambat termasuk kategori sedang sampai kuat. Rerata zona hambat ED pada S. aureus lebih besar daripada S. pyogenes yang berbeda bermakna menurut uji one-way ANOVA, uji post-hoc Duncan dan uji T independent. Aktivitas antibakteri pada ekstrak masih dibawah kontrol positif. Kesimpulannya, ekstrak etanol daun X. granatum memiliki daya antibakteri terhadap S. aureus dan S. pyogenes, dengan daya hambat sedang sampai kuat.
KADAR HAMBAT MINIMAL DAN KADAR BUNUH MINIMAL EKSTRAK KULIT BATANG Xylocarpus granatum TERHADAP Staphylococcus aureus DAN Escherichia coli Shahiba Inayati Maghfira; Agung Biworo; Lia Yulia Budiarti; Erida Wydiamala; Joharman Joharman
Homeostasis Vol 6, No 2 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/ht.v6i2.10006

Abstract

Tanaman Xylocarpus granatum (X. granatum) memiliki potensi sebagai antimikroba.  Bagian kulit batangnya dilaporkan banyak mengandung senyawa antibakteri dan aktivitasnya dipengaruhi asal habitatnya. Penelitian ini menginformasikan daya antibakteri X. granatum asal Hutan Mangrove Pulau Burung, Kalimantan Selatan. Penelitian eksperimental secara in vitro ini menggunakan metode dilusi, mengujikan ekstrak etanol kulit batang X. granatum (EKBXG) 3,125%, 6,25%, 12,5%, 25%, 50%, dan 100% terhadap Staphylococcus aureus ATCC 25923 (S. aureus) dan Escherichia coli ATCC 25922 (E. coli). Parameter penelitian diamati secara visual dan kultur hasil perlakuan EKBXG pada kedua bakteri uji adalah kadar hambat minimum (KHM) dan kadar bunuh minimum (KBM. Perlakuan dan pengamatan dilakukan pada 3 kali ulangan. Hasilnya didapatkan nilai KHM dari EKBXG terhadap S. aureus adalah 6,25% dan KBM 12,5% nilai KHM dari EKBXG terhadap E. coli adalah 12,5% dan KBM 25%. Simpulan, berdasarkan nilai KHM dan KBM, daya antibakteri ekstrak kulit batang Xylocarpus granatum terhadap Staphylococcus aureus adalah 6,25% dan 12,5% serta terhadap Escherichia coli adalah 12,5% dan 25%.
Co-Authors A.A. Ketut Agung Cahyawan W Abdurahman Wahid Agung Biworo Agung Biworo Agung Biworo Ahdadia, Huda Aisyah Aisyah Akbar Rihansyah Akbar Rihansyah, Akbar Alfi Yasmina Alfi Yasmina Alfi Yasmina Alfia Fitriani Alfia Fitriani, Alfia Aliy Arivin Anward Alshazil, Renata Seikh Amalia, Maulidia Khairada Amalya, Khalida Zikra Amiratun Naillah Anward, Aliy Arivin Ari Yunanto Arietama, George Armanda, Ferdio Aulia Nalar, Gusti Ayu Apriliani Ayu Dewi Pertiwi Ayu Septiana Azhari, Nazla Puteri Bhisma Ridho Romadhon Borneo Yuda Pratama Chyntia Devi, Made Putri Dastin Andre Davi’ Qowiyul Ali Dayana, Puteri Dayani, Nor Ella Debby Saputera, Debby Derlin, Ellanda Permata Desy Elisa Kismiliansari Desy Elisa Kismiliansari, Desy Elisa Devin, Firdi Dewi Nurdiana Dewi, Arisanty Dhian Ririn Lestari Dhian Ririn Lestari, Dhian Ririn Diah Puspita Rifasanti Diah Puspita Rifasanti Dini Permata Sari Dita Permatasari Dita Permatasari Dwi Nur Rachmah Dwi Nur Rachmah Edi Hartoyo Edi Hartoyo Edyson Edyson Edyson Edyson Edyson Edyson, Edyson Eka Yudha Rahman Eka Yudha Rahman Erida Widyamala Erida Wydiamala Erida Wydiamala Erwin Rosadi Erwin Rosadi, Erwin Fachriyad, Muhammad Fahdyannoor, Fahdyannoor Fahdyanoor Fahmi, Yafi Farida Heriyani Farida Heriyani Farida Heriyani Farida Raudah Farida Raudah, Farida Ferdio Armanda Galuh Eka Suryani Ghina Salsabila Gusti Aulia Nalar Hafiz Rakhmatullah Hafizhah, Ghina Hardiyanti Ruslan Hayatun Nufus Herawati Herawati Hikmah Ika Darmayanta Husna Dharma Putera Husna Dharma Putera, Husna Dharma Husnul Khatimah Husnul Khatimah Ichrom Nahzi, Muhammad Yanuar Ihsanti, Shofia Hilwa Ihya Ridlo Nizomy Ihya Ridlo Nizomy, Ihya Ridlo Ikhwanda Angga L. Ikhwanda Angga L., Ikhwanda Angga Indah Ramadhan Intan Kusuma Dewi Irma Zufira Isnaini Isnaini Isnaini Joharman Joharman Khatimah, Husnul Khatimah3, Husnul Lena Rosida Lie Vanny Leono Lutfia Papita Derizky Rahmayanti M. Rizki Valian Akbar M. Rizki Valian Akbar, M. Rizki Valian M. Yogi Riyantama Isjoni Maharani Laillyza Apriasari Maulida, Maulida Moehammad Rezaldi Panesa Mohammad Bakhriansyah Muhammad Baihaqi Siddik Muhammad Bayu Fernanda Muhammad, Fadil Muthmainah, Noor Nadhila Nadhila Nadya Azzahra, Nadya Nadya Salsabila Nafiah Syella, Nafiah Nafilah Syella Nafilah Syella, Nafilah Najiya Ulfa Nasution, Naulita Sari Nazla Puteri Azhari Nida Nurkhalishah Nisa, Rohmatun Noor Fathimah Zohra Noormuthmainah, Noormuthmainah Nor Admi Zayanti Nor Ella Ella Dayani Norma Sari Normaida Novianti Novita Pratiwi Nur Adnia Nur Azmina Aisyah Nur Qamariah Nur Salsabila Apriliani Risma Putri Nurlaili Rafina Nurwafa, Nurwafa Nurzahida, Gusti Nadya Pauline Surya Kurniati Prenggono, Muhamad Darwin Puspa Astri Sella Putra, Andifa Anugerah Putri Putri, Putri Qiptiah, Putri Mariatul Rahmah, Resvi Amalia Rahmiati Rahmiati Raymona Dewi Ginarti Ridhoni, Muhammad Zaki Rohmatun Nisa S., Nur Almira R. Safaana, Aurora Savitri, Dwiana Shahiba Inayati Maghfira Siddik, Muhammad Baihaqi Silvan Juwita Siti Kaidah Siti Kaidah Solly Aryza Sonya Esti Kholifa Sri Hayati Nufaliana Sri Hayati Nufaliana, Sri Hayati Sri Widyarsi Strata Pertiwi Strata Pertiwi, Strata Sukma Noor Akbar Talitha Maghfira Ramadhinta Talitha Maghfira Ramadhinta, Talitha Maghfira Thea Shagita Ulfa, Najiya Vania Puspitasari Sangadi Wahyuni A Wahyuni A, Wahyuni Widiantoro Saputro Widya Nursantari Wydiamala, Erida Wydiamala, Erida Yudi Yahya Yulia, Noor Zafira Aisyah Putri Zohra, Noor Fathimah Zufira, Irma