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Jumlah Bakteri Escherichia coli pada Ikan Lele (Clariasis gariepinus) Asap di Pasar Tradisional Kecamatan Simpang Kiri Kota Subulussalam (The Amount Of Escherichia coli Bacteria In Smoked Catfish (Clariasis gariepinus) In Traditional Markets Simpang Kiri District Subulussalam Municipality ) Ega Wilia Fitri; Rastina Rastina; Fakhrurrazi Fakhrurrazi; Mahdi Abrar; Eliawardani Eliawardani; Teuku Zahrial Helmi
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 3 (2022): MEI-JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i3.17467

ABSTRAK Ikan lele adalah ikan air sungai yang mengandung nutrisi yang tinggi. Kandungan gizi pada ikan lele yaitu lemak (4,8 %), mineral (1,2 %), protein (17,7 %) dan air (76 %). Ikan lele mudah membusuk sehingga perlu dilakukan pengolahan yang baik untuk menjaga kualitas ikan. Salah satu cara pengolahan untuk mencegah ikan membusuk yaitu dengan diasapkan. Penelitian ini bertujuan untuk membuktikan cemaran Escherichia coli pada ikan lele asap yang ada di Kecamatan Simpang Kiri Kota Subulussalam memenuhi Standar Nasional Indonesia (SNI) atau tidak. Sampel penelitian ini adalah ikan lele asap dari lima penjual. Dua ekor ikan lele asap dari setiap penjual sehingga total ikan berjumlah 10 ekor ikan lele asap. Perhitungan jumlah Escherichia coli menggunakan metode TPC (Total Plate Count). Sampel ikan dari setiap penjual ditimbang sebanyak 5g/ sampel kemudian dilakukan pengenceran menggunakan larutan Buffer Pepton Water (BPW) konsentrasi 0,1% dan diperiksa menggunakan uji TPC menggunakan media Eosin Methylen Blue Agar (EMBA). Data hasil pengamatan dianalisis secara deskriptif. Hasil penelitian menunjukkan seluruh sampel ikan dari lima pedagang didapatkan hasil positif pada media. Semua sampel ikan dari lima pedagang ikan lele asap di Pasar Tradisional Kecamatan Simpang Kiri Kota Subulussalam menunjukkan hasil positif tercemar bakteri Escherichia coli dan belum memenuhi SNI sehingga perlu untuk ditinjau kembali pada proses pengolahan serta cara penjualan. Kata kunci : Clariasis gariepinus, Escherichia coli, Kota Subulussalam, analisis deskriptif. ABSTRACT Catfish is a river water fish that contains high nutrients. The nutritional content of catfish is fat (4.8%), minerals (1.2%), protein (17.7%) and water (76%). Catfish is easy to rot so it needs good processing to maintain the quality of the fish. One way to prevent fish from rotting is by smoking it. This study aims to prove that Escherichia coli contamination in smoked catfish in Simpang Kiri District, Subulussalam City meets the Indonesian National Standard (INS) or not. The sample of this research is catfish as soon as possible from the five merchants. Two catfish as fast as possible from each merchants so that the total fish can catch 10 catfish as fast as possible. The calculation of the number of Escherichia coli uses the TPC (Total Plate Count) method. Fish samples from each merchant were weighed as much as 5g / sample then dilution was carried out using a 0.1% concentration of Buffer Pepton Water (BPW) solution and the cost using the TPC test using Eosin Methylene Blue Agar (EMBA) media. The data from the observations were descriptive. The results showed that all fish samples from five merchants had positive results on the media. All fish samples from smoked catfish merchants in the Traditional Market of Simpang Kiri Sub-district, Subulussalam City showed positive results contaminated with the Escherichia coli bacteria and were not Indonesian Nasional Standard so it is necessary to review the processing and sales methods. Keywords: Clariasis gariepinus, Escherichia coli, Subulussalam City, descriptive analysis.

PENENTUAN SUBTIPE VIRUS AVIAN INFLUENZA DENGAN METODE SINGLE STEP MULTIPLEX REVERSE TRANSCRIPTASE- POLYMERASE CHAIN REACTION (RT-PCR) ISOLAT ASAL PROVINSI ACEH Teuku Zahrial Helmi; Rini Widayanti; Aris Haryanto
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Tujuan dari penelitian ini adalah mengidentifikasi keberadaan gen M, H5, dan N1 virus avian influenza (AI) melalui metode single step multiplex reverse transcriptase-polymerase chain reaction (RT-PCR), sebagai acuan untuk peneguhan diagnosis secara molekuler virus AI di Provinsi Aceh. Penelitian ini menggunakan 11 isolat virus AI asal Provinsi Aceh yang diperoleh dari Laboratorium Balai Penyidikan dan Pengujian Veteriner (BPPV) Regional I Medan di Sumatera Utara dari tahun 2006-2008. Penelitian dilakukan di Laboratorium Virologi BPPV Regional I di Medan, Sumatera Utara. Amplifikasi terhadap gen matriks (M) virus AI menggunakan metode simplex RT-PCR. Hasil simplex RT-PCR terhadap gen M diperoleh 10 isolat yang menunjukkan pita deoxyribonucleic acid (DNA) pada 276 bp dan satu isolat yang tidak muncul, kemudian dilanjutkan dengan metode single step multiplex RT-PCR menggunakan pasangan primer gen penyandi protein N1, H5, dan M. Produk PCR 131 bp (N1), 189 bp (H5), dan 276 bp (M) muncul sebagai hasil elektroforesis dari semua isolat virus AI. Semua virus AI yang mewabah dari tahun 2006-2008 di Provinsi Aceh termasuk ke dalam virus influenza A subtipe H5N1.

ISOLASI DAN IDENTIFIKASI VIRUS AVIAN INFLUENZA PADA BERBAGAI SPESIES UNGGAS SECARA SEROLOGIS DAN MOLEKULER (Isolation and Identification of Avian Influenza in Different Species of Poultry by Means of Serological and Molecular Methods) Teuku Zahrial Helmi; Charles Rangga Tabbu; Wayan Tunas Artama; Aris Haryanto; Muhammad Isa
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

The purpose of this research was to identify avian influenza (AI) virus using serological and molecular methods on poultry which suspected as AI infected in Aceh province. This study used 37 samples of tracheal and cloacal swabs and organs from various species of poultry that were collected from several districts/cities in Aceh. Samples were collected and put into transport media and stored at 4° C before sending to the laboratory. Samples were inoculated in specific pathogen-free of embryonated chicken egg with the age of 9-11 days for further serological and molecular examination. From 37 samples which infected to embryonated chicken egg then followed by hemagglutinin agglutination test/hemagglutinin inhibition revealed that 7 samples were positively infected with AI virus. The amplification result of specific matrix gene primer was followed by electrophoresis on 2% agarose gel which were obtained in the form of a deoxyribonucleic acid (DNA) band at 276 bp for matrix gene and 1.725 bp for H5 gene for all isolates test. In conclusion, the virus which caused the death of various types of poultry in Aceh province is avian influenza A virus subtype H5.Key words: avian influenza virus, H5N1, serologic, matrix, heamaglutinin

The Relationship between Farmer Characteristic and Knowledge towards the Successfulness of Cattle Artificial Insemination (AI) in Aceh Besar District Julia Kardin; Dasrul Dasrul; Sugito Sugito; Nurliana Nurliana; Teuku Zahrial Helmi
The International Journal of Tropical Veterinary and Biomedical Research Vol 3, No 1 (2018): Vol. 3 (1) May 2018
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

The purpose of this research was to identify the relationship of characteristic and knowledge of farmers to the successful of artificial insemination (AI) in Aceh Besar Districts. The number of respondents is 93 people from twenty three districts in Aceh Besar, selected purposively as the sample of this research. The instruments used in this research are questionnaire and direct observation in the field, while the observed variables are: farmer characteristics, farmer knowledge and artificial insemination result. The results of this research revealed that the characteristics and knowledge of the farmers were good and showed a real relationship to the successfulness of AI in Aceh Besar Districts. The average service per conception (S / C) in Aceh Besar Districts during 2017 was 1.32 while the conception rate (CR) was 86.36%. The results were in accordance with the standard of general guidance of Upsus Siwab Year 2017.

Cellulase activity of bacteria isolated from water of mangrove ecosystem in Aceh Province Irma Dewiyanti; Darmawi Darmawi; Zainal Abidin Muchlisin; Teuku Zahrial Helmi; Iko Imelda Arisa; Cut Nanda Defira; Fitriyani Fitriyani; Sawva Yura
Depik Vol 10, No 3 (2021): December 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Cellulolytic bacteria that produce cellulase enzymes play an essential role in degrading cellulose in their habitat. The presence of cellulolytic bacteria strongly supports the fertility and productivity in mangrove waters. The objectives of the study are to analyze the activity of cellulase enzyme qualitatively through the cellulolytic index and quantitatively through the activity and specific activity of the cellulase enzyme from bacteria isolated from the water of mangrove ecosystems in Aceh Province. The qualitative experiment of enzyme activity was carried out at the Microbiology laboratory SKIPM Aceh, and a quantitative experiment of enzyme activity was conducted at the Microbiology Laboratory, Biology Department, IPB. Isolation of cellulolytic bacteria isolated from mangrove water used Carboxy Methyl Cellulose (1% CMC) selective media and carried out by spread plate method. The ability of bacteria to produce cellulase was tested qualitatively using the spot technique, this test was carried out using 1% Congo Red. Furthermore, the quantitative testing of cellulase enzymes activity adopted the DNS spectrophotometric method. The specific activity of the cellulase enzyme can be determined by using the Lowry method. There were 21 isolates that had a clear zone and had the ability to produce cellulase enzymes from 49 isolates that were successfully purified. The highest cellulolytic index (CI) produced using BAM421 isolate with the value of 5.50 was included in the high category, followed by BAM326 and BAM132 isolates, with values of 1.55 and 1.05 were categorized into the medium category. The other isolates were in the low cellulolytic index category. The isolate with the highest CI value was further tested using the quantitative enzyme activity test. The highest cellulase enzyme activity of BAM421 occurred at 24hr (0.0029 U/ml). The highest specific cellulase activity of BAM421 was at 24hr with the value of 0.210 U/mg. The result concluded that the qualitative test showed CI values can be categorized into low, medium, and high. Moreover, the value of the quantitative assay described that the cellulase enzyme and the specific enzyme activities of the bacteria were low in the study area.Keywords:Cellulolytic indexQuantitative testMangrove watersCellulase enzymeMicroorganismTRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian // TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//

Cellulase activity of bacteria isolated from water of mangrove ecosystem in Aceh Province Irma Dewiyanti; Darmawi Darmawi; Zainal Abidin Muchlisin; Teuku Zahrial Helmi; Iko Imelda Arisa; Cut Nanda Defira; Fitriyani Fitriyani; Sawva Yura
Depik Vol 10, No 3 (2021): December 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.3.22964

Cellulolytic bacteria that produce cellulase enzymes play an essential role in degrading cellulose in their habitat. The presence of cellulolytic bacteria strongly supports the fertility and productivity in mangrove waters. The objectives of the study are to analyze the activity of cellulase enzyme qualitatively through the cellulolytic index and quantitatively through the activity and specific activity of the cellulase enzyme from bacteria isolated from the water of mangrove ecosystems in Aceh Province. The qualitative experiment of enzyme activity was carried out at the Microbiology laboratory SKIPM Aceh, and a quantitative experiment of enzyme activity was conducted at the Microbiology Laboratory, Biology Department, IPB. Isolation of cellulolytic bacteria isolated from mangrove water used Carboxy Methyl Cellulose (1% CMC) selective media and carried out by spread plate method. The ability of bacteria to produce cellulase was tested qualitatively using the spot technique, this test was carried out using 1% Congo Red. Furthermore, the quantitative testing of cellulase enzymes activity adopted the DNS spectrophotometric method. The specific activity of the cellulase enzyme can be determined by using the Lowry method. There were 21 isolates that had a clear zone and had the ability to produce cellulase enzymes from 49 isolates that were successfully purified. The highest cellulolytic index (CI) produced using BAM421 isolate with the value of 5.50 was included in the high category, followed by BAM326 and BAM132 isolates, with values of 1.55 and 1.05 were categorized into the medium category. The other isolates were in the low cellulolytic index category. The isolate with the highest CI value was further tested using the quantitative enzyme activity test. The highest cellulase enzyme activity of BAM421 occurred at 24hr (0.0029 U/ml). The highest specific cellulase activity of BAM421 was at 24hr with the value of 0.210 U/mg. The result concluded that the qualitative test showed CI values can be categorized into low, medium, and high. Moreover, the value of the quantitative assay described that the cellulase enzyme and the specific enzyme activities of the bacteria were low in the study area.Keywords:Cellulolytic indexQuantitative testMangrove watersCellulase enzymeMicroorganismTRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian // TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//

Cellulase activity of bacteria isolated from water of mangrove ecosystem in Aceh Province Irma Dewiyanti; Darmawi Darmawi; Zainal Abidin Muchlisin; Teuku Zahrial Helmi; Iko Imelda Arisa; Cut Nanda Defira; Fitriyani Fitriyani; Sawva Yura
Depik Vol 10, No 3 (2021): December 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.3.22964

Cellulolytic bacteria that produce cellulase enzymes play an essential role in degrading cellulose in their habitat. The presence of cellulolytic bacteria strongly supports the fertility and productivity in mangrove waters. The objectives of the study are to analyze the activity of cellulase enzyme qualitatively through the cellulolytic index and quantitatively through the activity and specific activity of the cellulase enzyme from bacteria isolated from the water of mangrove ecosystems in Aceh Province. The qualitative experiment of enzyme activity was carried out at the Microbiology laboratory SKIPM Aceh, and a quantitative experiment of enzyme activity was conducted at the Microbiology Laboratory, Biology Department, IPB. Isolation of cellulolytic bacteria isolated from mangrove water used Carboxy Methyl Cellulose (1% CMC) selective media and carried out by spread plate method. The ability of bacteria to produce cellulase was tested qualitatively using the spot technique, this test was carried out using 1% Congo Red. Furthermore, the quantitative testing of cellulase enzymes activity adopted the DNS spectrophotometric method. The specific activity of the cellulase enzyme can be determined by using the Lowry method. There were 21 isolates that had a clear zone and had the ability to produce cellulase enzymes from 49 isolates that were successfully purified. The highest cellulolytic index (CI) produced using BAM421 isolate with the value of 5.50 was included in the high category, followed by BAM326 and BAM132 isolates, with values of 1.55 and 1.05 were categorized into the medium category. The other isolates were in the low cellulolytic index category. The isolate with the highest CI value was further tested using the quantitative enzyme activity test. The highest cellulase enzyme activity of BAM421 occurred at 24hr (0.0029 U/ml). The highest specific cellulase activity of BAM421 was at 24hr with the value of 0.210 U/mg. The result concluded that the qualitative test showed CI values can be categorized into low, medium, and high. Moreover, the value of the quantitative assay described that the cellulase enzyme and the specific enzyme activities of the bacteria were low in the study area.Keywords:Cellulolytic indexQuantitative testMangrove watersCellulase enzymeMicroorganismTRANSLATE with x EnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian // TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//

3. Efficacy of Jatropha (Jatropha curcas L) Cream sap Leucocytes in Inflammation Phase of Wound Healing Mita Nasuha Risky; Triva Murtina Lubis; M. Nur Salim; Teuku Zahrial Helmi; Abdul Harris; Hennivanda Hennivanda; Dian Masyitha; Cut Dahlia Iskandar; Fitriani Fitriani; Abdul Hamzah; T. Fadrial Karmil
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.19092

This study aims to determine the efficacy of giving jatropha cream (Jatropha curcas L.) on the number of leukocytes in the inflammatory phase of wound healing in the mice’s skin. The method used is literature study research with qualitative techniques. The data used in this study comes from the result of studies that have been conducted and published in national or international online journals. References used are all research articles with data selection. The results of data analysis showed that the administration of jatropha cream (Jatropha curcas L.) was effective in limiting or inhibiting the accumulation of leukocytes in the area of inflammation and could stimulate the formation of new blood vessels (angiogenesis) in the inflammatory phase of wound healing of the mice’s skin. Based on these data it can be concluded that the efficacy of jatropha cream (Jatropha curcas L.) affects the number of leukocytes in the inflammatory phase of wound healing of mice's skin.

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