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ISOLASI DAN KARAKTERISASI ISOLAT BAKTERI RESISTEN TEMBAGA DARI SUNGAI CISADANE Wahyu Irawati; Candra Yulius Tahya
BERITA BIOLOGI Vol 19, No 3B (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3B.3937

Abstract

The water quality of the Cisadane River, located in the industrial factory area, it is contaminated with copper, it is necessary to treat sewage. Biological wastewater treatment using bacteria is a more economical and effective method of removing copper from the environment than chemical methods. This study aims to isolate, identify, determine the level of resistance, and analyze the ability of bacteria to accumulate copper. Isolation was carried out by the spread method in Luria Bertani Agar medium containing CuSO4. Bacterial isolates were identified based on analysis of the 16S rDNA gene. The resistance level is determined based on the Minimum Inhibitory Concentration (MIC) value. The accumulation ability of copper was determined using an atomic absorption spectrophotometer. Six bacteria resistant to copper were isolated and identified as Pantoea agglomerans strain IrCis1, Klebsiella grimontii strain IrCis3, Klebsiella penumoniae strain IrCis4, Shigella flexneri strain IrCis5, Enterobacter cloacae strain IrCis6, and Enterobacter cloacae strain IrCis9 with MIC of 7 mM - 9 mM. Pantoea agglomerans strains IrCis1, Klebsiella grimontii strains IrCis3, Klebsiella pneumoniae strains IrCis4 can accumulate copper of 1,19 mg, 1,39 mg, and 0,92 mg, respectively, per gram of dry cell weight.  
Praktikum sederhana di rumah tentang pengaruh penggunaan Hand Sanitizer terhadap keberadaan koloni bakteri di tangan Wahyu Irawati
Jurnal Pendidikan Biologi undiksha Vol. 8 No. 3 (2021)
Publisher : Undiksha

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bakteri adalah mikroorganisme yang mampu berkembang biak dengan cepat dan berada di setiap tempat. Tangan merupakan salah satu bagian yang banyak didiami bakteri. Pada masa pandemi, penggunaan hand sanitizer menjadi sangat dibutuhkan karena bersifat praktis. Masyarakat menggunakan hand sanitizer untuk menghentikan laju pertumbuhan bakteri di tangan. Hand sanitizer merupakan cairan dengan kandungan etanol dan bahan aktif lainnya yang mampu mensterilkan tangan dan merusak membran sel bakteri. Penelitian ini dilakukan dengan praktikum sederhana menggunakan alat dan bahan yang ada dirumah. Praktikum sederhana dilakukan dengan pembuatan medium sintesis sebagai media pertumbuhan bakteri dengan memanfaatkan alat dan bahan yang ada di sekitar rumah serta pengamatan koloni bakteri di telapak tangan setelah penggunaan hand sanitizer. Kegiatan yang dilakukan adalah membandingkan koloni bakteri di (1) medium sintesis tanpa perlakuan, (2) medium sintesis yang ditempeli telapak tangan tanpa penggunaan hand sanitizer dan (3) medium sintesis yang ditempeli telapak tangan sesudah menggunakan hand sanitizer. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan hand sanitizer terhadap keberadaan koloni bakteri di tangan. Hasil praktikum menunjukkan bahwa pertumbuhan bakteri di setiap medium dengan perlakuan berbeda menghasilkan jumlah koloni yang berbeda-beda. Bakteri lebih banyak tumbuh pada medium dengan perlakuan tanpa hand sanitizer sedangkan penampakan koloni bakteri menurun setelah tangan menggunakan hand sanitizer. Berdasarkan hasil praktikum dapat disimpulkan bahwa penggunaan hand sanitizer efektif untuk mengurangi keberadaan koloni bakteri di tangan. Saran untuk praktikum sederhana selanjutnya yaitu mencoba beberapa jenis hand sanitizer untuk menentukan jenis hand sanitizer terbaik yang dapat meminimalisir jumlah bakteri di tangan.
Peran Guru Sebagai Teladan Dalam Upaya Pembentukan Karakter Siswa Berdasarkan Kajian Filsafat Aksiologi Kristen Helen Melenia Sianipar; Wahyu Irawati
Didache: Journal of Christian Education Vol 3, No 1 (2022): Juni 2022
Publisher : Sekolah Tinggi Teologi Simpson Ungaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46445/djce.v3i1.483

Abstract

Character is a basic thing that must be owned by students as the basis for ways of thinking and behaving and reflecting their identity. However, the facts show that many students experience a character crisis. This is shown through bullying and undisciplined and irresponsible attitudes that show that it is very important to build student character. Based on the philosophy of Christian axiology, ethics has an important role in the process of building students' characters who are more like Christ. Students as representatives of God are expected to have good character according to the values of truth and reflect the character of Christ. However, they fall into sin has caused humans to tend to take actions that are contrary to the values of truth. This also happens to students who are shown through acts of bullying, stealing, cheating, and other negative things. The purpose of this paper is to examine the role of the teacher as a role model to build student character based on a review of Christian axiological philosophy by using the literature review method. The results obtained indicate that the role of Christian teachers as role models is very important in efforts to build student character. Christian teachers who have been born again and are led by the Holy Spirit will be enabled to understand axiological values based on Bible truth so that they can provide concrete examples to shape students' character in the form of exemplary attitudes and actions. It is recommended for further research to examine and analyze more deeply the role of the teacher as a role model in the building of student character.
Pelatihan Bioteknologi dan Aplikasinya di Sekolah Menengah Atas (SMA) Erenos Reisky Tammu; Wahyu Irawati; Jessica Elfani Bermuli; Keisha Hillary Pangalila; Lusiana Haris
Lumbung Inovasi: Jurnal Pengabdian kepada Masyarakat Vol. 7 No. 2 (2022): June
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/linov.v7i2.654

Abstract

Sekolah Menegah Atas (SMA) Erenos memiliki visi yaitu membangun manusia seutuhnya dengan fokus pada Kristus sehingga menghasilkan lulusan yang beriman, berilmu dan memiliki karakter Kristiani serta mendatangkan damai sejahtera bagi diri sendiri dan sesama manusia. Salah satu misi dari SMA Erenos yaitu menumbuh kembangkan budaya ingin tahu, gemar belajar, menganalisis, kerja keras, melakukan percobaan dan kreatif. Permasalahan yang muncul adalah SMA Erenos ingin Pengabdian kepada Masyarakat di SMA Erenos bertujuan agar para siswa mampu menjelaskan konsep dasar bioteknologi dengan benar dikaitkan dengan peran manusia sebagai mahkota ciptaan Allah serta memahami contoh penerapan bioteknologi dalam bidang pangan/pertanian dan lingkungan dengan tepat. Kegiatan ini dilakukan dengan metode ceramah secara online dengan menggunakan aplikasi zoom. Hasil kegiatan PkM menunjukkan bahwa 86.34% siswa dapat memahami dengan mudah konsep dasar Bioteknologi, 81.95% mengatakan bahwa contoh atau aplikasi dari penerapan Bioteknologi juga disampaikan dengan baik oleh narasumber. Dari segi keterkaitan ilmu, 71.95% mengatakan bahwa penyampaian keterkaitan Bioteknologi, Genetika, dan Mikrobiologi tersampaikan dengan jelas. Kemudian 84,88% siswa mengatakan bahwa mereka mendapatkan pengetahuan baru terkait teknologi dari materi yang dipaparkan, dan 80% siswa semakin memahami mengenai peran manusia sebagai mahkota ciptaan dalam menjalankan mandat budaya, secara khusus pada bidang Bioteknologi. Training About Biotechnology and Its Applications in the Erenos Middle High School Erenos High School (SMA) has a vision that is to build a complete human being with a focus on Christ so as to produce graduates who are faithful, knowledgeable, and have Christian character and bring peace to themselves and fellow human beings. One of the missions of SMA Erenos is to cultivate a culture of curiosity, love of learning, analysis, hard work, experimentation, and creativity. The problem that arises is that Erenos High School wants to foster a culture of curiosity and love for learning for high school students. Erenos High School wants to realize learning that can open up students’ insight in depth to overcome the lack of connection between the topics discussed in class and their connection with the latest scientific developments, especially regarding biotechnology. Community Service at Erenos High School aims to enable students to be able to properly explain the basic concepts of biotechnology related to the role of humans as the crown of God’s creation and to understand examples of the application of biotechnology in the field of food/agriculture and the environment correctly. This activity is carried out by means of lectures online using the zoom app followed by a question and answer session. The activity was held on Monday, December 6, 2021, at 08:00-09.00 WIB with a total of 47 participants. The results of the PkM activity showed that 86.34% of students could easily understand the basic concepts of Biotechnology, and 81.95% said that examples or applications of the application of Biotechnology were also delivered well by the speakers. In terms of the relevance of science, 71.95% said that the delivery of the linkage of Biotechnology, Genetics, and Microbiology was conveyed clearly. Then 84.88% of students said that they got new knowledge related to technology from the material presented, and 80% of students increasingly understood the role of humans as the crown of creation in carrying out cultural mandates, specifically in the field of Biotechnology.      
Penanganan Epilepsi Grand Mal Sebagai Akibat dari Ketidaknormalan Kerja Neuron di Otak Menggunakan Metode Diet Ketogenik Nommensen Pangihutan Ompusunggu; Sabar Manampin Giawa; Wahyu Irawati
BIOEDUSCIENCE Vol 6 No 1 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (618.578 KB) | DOI: 10.22236/j.bes/617688

Abstract

Background: The nervous system is composed of a complex structure to support its highly organized work. Damage to the working mechanism of nervous systems can exert fatal impacts to the human body. One of the consequences of damage to the mechanism of action potentials is grand mal epilepsy. The purpose of this paper is to determine the structure, function, and mechanism of action of neurons, establish grand mal epilepsy as a result of damage to the working mechanism of neurons, and recognize the ketogenic diet as a non-pharmacological therapy for epilepsy patients. Methods: The research method used is descriptive qualitative research. Results: Nerve cells generally consist of dendrites for receiving stimuli, cell bodies for transmitting stimuli to the axon, and axons for transmitting stimuli to other nerve cells. Grand mal epilepsy is a nervous system disorder caused by the increased activity of sending electrical waves throughout the brain that can reach up to four times faster than normal. However, grand mal epilepsy patients suffer from an excessive number of seizures that may occur at any time. Conclusions: The ketogenic diet method is a high-fat and low-carbohydrate diet method that can reduce electrical activity in the brain. Ketone-containing fats have a stabilizing effect on the central nervous system, contributing to anticonvulsant and antiepileptic effects that may reduce the excessive number of seizures experienced by patients. The application of this diet should be balanced with the provision of additional intake of multivitamins, minerals, and fiber to balance the nutrients the body needs.
Anti-PD-L1 Therapy as a Solution for Non-Melanoma Skin Cancer Basal Cell Carcinoma Rahul Simon Situmeang; Gim Mi Kyong; Rosiva Betaria Purba; Wahyu Irawati
BIOEDUSCIENCE Vol 6 No 1 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (615.256 KB) | DOI: 10.22236/j.bes/618095

Abstract

Skin cancer is a disease that can cause the loss of the ability to regenerate and protect the skin normally. The types of skin cancer that are known are melanoma and non-melanoma skin cancer. Non-melanoma Basal cell carcinoma is a malignant neoplasm originating from non-keratinizing cells in the basal layer of the skin's epidermis. The treatment itself is carried out through the application of immunotherapy, namely the use of drug therapy Programmed Death Ligand 1 (PD-L1). This study aims to see the effectiveness of PD-L1 Therapy as a treatment for basal cell carcinoma. The focus of the study of the article is the integument system, skin cancer, non-melanoma basal cell carcinoma, and the effect of anti-PD-L1 use. The research method used is a literature review from various sources. Skin cancer Basal cell carcinoma attacks the basal cells of the epidermis, causing disruption. Attacks slowly, but when ignored will spread wider and more severe. Through PD-L1 therapy by binding to PD-1 receptors on immune cells, it causes the activation of T lymphocytes as anti-cancer cells in the body that suppress growth while actively controlling tumor cells. Through the use of PD-L1 therapy in treating cancer that attacks basal cells, it will suppress growth, destroy and shrink cancer cells, and increase the body's immunity against cancer cells.
Andaliman (Zanthoxylum Acanthopodium DC.), a Rare Endemic Plant from North Sumatra that Rich in Essential Oils and Potentially as Antioxidant and Antibacterial Nommensen Pangihutan Ompusunggu; Wahyu Irawati
Jurnal Biologi Tropis Vol. 21 No. 3 (2021): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i3.2961

Abstract

Andaliman (Zanthoxylum acanthopodium DC.) is one of the endemic plants originating from North Sumatra. This plant is known for its benefits as a special seasoning spice in Batak tribe community. Humans as the image of God are given the ability to conduct an investigation and development of what God has created so that science begins to develop to investigate the potential of andaliman plants in the field of health. This writing aims to determine: 1) morphological and physiological characteristics of andaliman, 2) habitat of andaliman, and 3) utilization of andaliman as antioxidant and antibacterial agent. The method of writing used in the preparation of this article is the study of literature. The results of the literature study show that the andaliman plant has a tap root, woody stems, green, odd-numbered and jagged-edged compound leaves, pale yellow flowers, pepper-shaped fruits, and shiny black or wrinkled black seeds with thick and hard seed shells. Andaliman habitat is a place with an altitude of 1200-1500 masl with an average temperature of 15-18 ̊ C and rainfall of 800-1000 mm/year and soil pH 5.5-7.6. Andaliman fruit contains 29 components of essential oils such as geranyl, cytronella, β-cytronelol, and nerol which have antioxidant activity. Andaliman fruit extract also has antibacterial activity, which inhibits the growth of microbes such as Escherichia coli, Salmonella typhimurium, Bacillus cereus, Pseudomonas fluorescens, Aspergillus flavus, Propionibacterium acnes and Staphylococcus aureus.
Potential of Ideonella sakaiensis bacteria in Degrading Plastic Waste Type Polyethylene Terephthalate Sherina Juliana; Mia Parhusip; Argoby Simanullang; Elisabeth Tita; Wahyu Irawati
Jurnal Biologi Tropis Vol. 22 No. 2 (2022): April - June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i2.3321

Abstract

Polyethylene terephathalate (PET) is a type of plastic content that is commonly is difficult to degrade so that it has an impact on global environmental problems. Plastic waste pollution needs to be overcome by using environmentally friendly methods to accelerate the PET plastic biodegradation process. Biodegradation is the use of microorganism activity to decompose plastic compounds so as to reduce the volume of waste. Ideonella sakaiensis is a bacterium that produces PETase enzymes that play a role in degrading PET. This literature review aims to determine the potential of Ideonella sakaiensis encoding the PETase gene in degrading polyethylene terephathalate plastic waste by discussing three focus studies, namely: 1) the potential of Ideonella sakaiensis, 2) the characteristics of polyethylene terephathalate plastic waste, 3) the relationship between the PETase coding gene and the degradation of polyethylene plastic waste terephathalate. Biodegradation of plastic waste Polyethylene terephathalate using Ideonella sakaiensis which has the potential to degrade PET faster with the PETase enzyme. The biodegradation mechanism carried out involves the process of transforming the PETase coding gene from I. sakaiensis to Escherichia coli bacteria influenced by pH and the effectiveness of the PETase enzyme work until the use of harvested products interacts with Polyethylene terephathalate plastic waste, which decomposes plastic waste. 
Use of the GnRH Antagonist Elagolix for Endometriosis in Relation to Hypoestrogenic Effect in Women Keisha Hillary Pangalila; Silvana Putri Tarigan; Viona Heryani Arla; Wahyu Irawati
Jurnal Biologi Tropis Vol. 22 No. 2 (2022): April - June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i2.3344

Abstract

The female reproductive system is a system related to reproduction, which includes hormones and reproductive organs. Endometriosis is a disease that attacks the female reproductive organs. Endometriosis is the growth of endometrial cells that are not in place so that it interferes with the female reproductive system. Endometriosis causes pelvic pain and infertility in women. Endometriosis can be treated in several ways. One is to use drugs such as the GnRH antagonist Elagolix. This study aims to find out more about endometriosis and the Elagolix treatment in dealing with it. The method used in this study is a literature review method by discussing four focus studies to achieve the objectives of the study. Endometriosis is caused by several causes such as genetics, immunology, retrograde menstruation, coelomic metaplasia and vascular and lymphatic spread. The result of Endometriosis is the occurrence of dysmenorrhea, dyspareunia to infertility for the sufferers. The treatment that can be applied to endometriosis is surgical and medical surgery. One treatment with medical therapy is using Elagolix to inhibit the growth of endometriosis tissue by suppressing the hormone estrogen. Through this research, researchers are grateful that humans are given reason and thoughts by God so that humans can further research endometriosis and continue to try and try various alternative treatments to cure sufferers. The knowledge gained by humans is also very helpful for each other, namely the women sufferers so that they can live a better life to get rid of endometriosis. The conclusion obtained is that Elagolix is ​​one of the latest alternative treatments that can treat endometriosis.
Phenol Biodegradation and Catechol 2,3-Dioxygenase Gene Sequencing of Bacillus cereus IrC2 isolated from Rungkut Indonesia Candra Yulius Tahya; Wahyu Irawati; Friska Juliana Purba
Jurnal Kimia Terapan Indonesia Vol 21, No 1 (2019)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (53.446 KB) | DOI: 10.14203/jkti.v21i1.415

Abstract

Phenol is toxic organic compounds that harmful to humans, mammals, and disrupt the aquatic environment, especially higher-organisms in fresh-water environment. The biodegradation method using bacteria to degrade hazardous chemical and detoxify wastewater is an effective and efficient method. Bacillus cereus IrC2 isolated from sludge in an industrial wastewater treatment plant in Rungkut – East Java, Indonesia has been examined for the ability to degrade phenols in minimal salt medium. Bacillus cereus IrC2 is Gram-positive bacterium. This bacterium is motile, rod-shaped and its nucleotides sequence of 16S rRNA gene has been sequenced and can be accessed in GenBank with accession number MK511840. Bacillus cereus IrC2 is capable to use phenol up to 400 ppm as the sole carbon source to grow for 48 hours incubation. Phenol degrades 96% from initial concentration. Degradation of phenol was calculated by colorimetric method using 4-aminoantipyrine reagent and confirmed by GC MS analysis. The aerobic degradation of phenol pathways consists of three steps; in the first step, two hydroxyl groups are inserted into aromatic ring and catalyzed by mono or dioxygenase to produce dihydroxy aromatic compounds which are mostly catechols. Catechol enters the next step of aromatic ring cleavage catalyzed by catechol 1,2-dioxygenase and/or catechol 2,3- dioxygenase. The catechol 2,3-dioxygenase gene of Bacillus cereus IrC2 has been amplified by PCR and cloned into pTA2 vector. The cloned plasmid (pTA2-catE) was transformed into E. coli DH5α and selected blue-white colonies. The insert sequence was determined by Sanger deoxy sequencing method. The catechol 2,3-dioxygenase gene nucleotides sequence of Bacillus cereus IrC2 was submitted nto GenBank with accession number MK561609.
Co-Authors . PATRICIA Aaron Hasthosaputro ABYATAR HUGO BASKORO Alvionita Thana Ambarita, Polin P. Ambarita, Polin Parulian Andara Frida Sheilaliany Daely Angelica, Apriliana Anggun Anggun Aninditya Sri Nugraheni Argoby Simanullang Argorby Simanullang Ashiong P Munthe Ashiong Parheheon Munthe Bellen Mouretta Bermuli, Jessica Elfani Beta Mualiman Laoli Bora, Merliana Ina Charles Immanuel Chelvieana A. Christanti Christeven Mevotema Christian, Grace Purnamasari Christian, Salomo Christine Febriandini Tinambunan Clement Khrisman Laia Clement Khrisman Laia Damai Yanti Manalu David Dharmawan Artha David L Gultom Debora V. Widyanti Delfania Apang Madao Dellania Yvonne Harefa Desi L. Sihombing Destya Waty Silalahi Dipakalyano Dipakalyano Dipakalyano, Dipakalyano Djoko Prasetyo Dominica Feni Valentina Dr. Adolf J.N. Parhusip Dwi N. Susilowati Egilia Lucky Eklesia Taysa Orah Elisabeth Tita Ellen Nur Endah Pangesti Elysabeth Simatupang Eunike Bunga Marvella Eunike Bunga Marvella Eunike Bunga Marvella Fernando Christofer Fernando Christofer Fernando Corneles Moniharapon Fiska Ivana Pratami Putri Tokede Floreta D. Icasari Frichilya Intan Lawa Padang Gaol, Riris Lumban Georgine Gladis Paula Sulardi Geraldy John Meyners Gim Mi Kyong Golda Santiur Sianturi Grace Charity Mary Cartir Greisnaningsi Greisnaningsi HARI HARTIKO Helen Melenia Sianipar Herna Siahaan Hernawati Siahaan Hutauruk, Kristian I Made Samudra Ice Frans Kosi Imanuel Adhitya Wulanata, Imanuel Adhitya Intan Cidarbulan Matita Iren Asima Situmorang Jennie Agatherania Listira JOEDORO SOEDARSONO Josephine Elsa Diani Putranto Karnelasatri, . Keisha Hillary Pangalila Krisdarwindah Mardiana Kristin Armis Pasaribu Laia, Clement Khrisman Lastiar R. Sitompul Lastiar Roselyna Sitompul, Lastiar Roselyna Lucia Kusumawati Lusiana Haris Maharaja, Dwi P. Manalu, Damai Y. Manalu, Damai Yanti Marcellie, Jessica Marvella, Eunike Bunga Melany Djami Mia Parhusip Muhamad Ananda Munthe, Ashiong P. Munthe, Sri Wahyu Ningsih Nadya Aurelia Ratna PutrI Naomi Dwany Naomi Dwany Nida Sopiah Nida Sopiah Nida Sopiah Nommensen Pangihutan Ompusunggu Nommensen Pangihutan Ompusunggu Novera, Rusliani S. Pahotkon Purba Pangaribuan, Medeliene Christine Patricia Patricia Pebrianti Pebrianti Pingkan Imelda Wuisan Polin Parulian Ambarita Ponagadi, Merry Adisty Prasetyo, Djoko Purba, Friska Juliana Rahmawati, Sintia Y. Rahul Simon Situmeang Rani Nurlina Tifen Raudhatunnur Reinhard Pinontoan Reisky Megawati Tammu Reisky Megawati Tammu Reisky Megawati Tammu Reisky Tammu Resky Megawati Tammu Resti Nopriyanti Rido, Elisabeth M.T. Rosiva Betaria Purba Ruben Patrick Adhiwijaya Ruth Y. Purba Sabar Manampin Giawa Salomo Christian Shaday, Vannesa El Sherina Juliana Sherina Juliana Siane Indriani, Siane Siane Indriyani Silaban, Grace Niche Agnesa Silalahi, Ramses Silvana Putri Tarigan Simamora, Tanti Marsanda Sindy Friska Siringo-ringo, Sisilya Situmorang, Stefani Situmorang, Widya Srigika Natalia Boru Ginting Stephanus Jordan Jans Steven Anggawinata Sudarsono Siburian Suhartono, Andreas Valiant Sukri, Urbanus Susi Sulistia Susi Sulistia, Susi Tabitha Grace Larasati Tahya, Candra Tahya, Candra Yulius Takashi Reymond Rimpan Tammu, Reisky Tammu, Reisky Megawati Torreno, Jovan Nathanael Tri P. Priyatno Triwibowo Yuwono Vania Austine Callista Timotius Vannesa El Shaday Ruth Advenita Viona Heryani Arla Waruwu, A'azokhi Xaveria, Jessica Yadi Suryadi Yantra Wijaya Yeni Angelina Zega YENNY SORAYA Yesica C. Rhenata Yohana Ayustin Yohana Delvia Ayustin Yohanes Edi Gunanto Yovanka P.P Tumakaka Zebua, Satria Putri Aryani