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THE EFFECT OF PASTEURIZATION STAGE ON THE PRODUCTION OF STRAW MUSHROOM (VOLVARIELLA VOLVACEA, BULL. EX. FR./SING.) Jessica Elfani Bermuli; Wahyu Irawati; Reisky Megawati Tammu
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 8 No. 2 (2022): February 2022
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i2.6167

Abstract

God created Indonesia with a tropical climate that is very appropriate to support the development of straw mushroom cultivation (Volvariella volvaceae). One of the stages of mushroom cultivation sequentially is pasteurization. This study aims to determine the effect of pasteurization stages, the accuracy of the pasteurization temperature, and the factors that influence the growth of straw mushrooms. The method in this study was carried out using livestock manure, with a composition of 400 gr goat manure and 500 gr rabbit manure. The pasteurization stage is maintained at a temperature of 60-700C with a humidity of 80-90%. The watering interval at the observation stage was 4 days with a frequency of 40% in experiment 1 and every day or a frequency of 100% in experiment 2. The results showed that the pasteurization stage was very influential on the mushroom growing media. Inaccuracy in pasteurization resulted in many weeds that thrived, thus inhibiting the growth of straw mushrooms. The optimal temperature in the pasteurization stage is 60-900C. The factors that influence the growth of mushroom are the selection of materials as fertilizers for mushroom cultivation, temperature, humidity include interval and frequency of watering, pasteurization process, weeding the contaminants, and time of observation.
THE POTENTIAL OF MICRO-CHINESE MEDICINE OSMOTHERAPY USING NANOPARTICLES AS A TREATMENT FOR CHRONIC KIDNEY DISEASE Alvionita Thana; Fernando Corneles Moniharapon; Kristin Armis Pasaribu; Sindy Friska; Wahyu Irawati
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 9 No. 1 (2022): August 2022
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i1.6358

Abstract

The kidneys are the most important to excretory organs because they have function to remove waste products from the body has metabolism. In the excretory system, the damage that could occur is the kidney has decreased until finally unable to perform its function properly which is called Chronic Kidney Disease (CKD). Chronic Kidney Disease caused by age, gender, and a history of diseases such as diabetes. One of the nanoparticle therapies that could be given to patients with chronic kidney disease is Micro-Chinese Medicine Osmotherapy. The purpose of this paper is to determine: 1) structure and how they work of the kidney and its relation to the urinary system, 2) causes and effects of chronic kidney disease on the urinary system, 3) mechanism of the urinary system in conditions of chronic kidney disease, and 4) mechanism and results of Micro-Chinese Medicine Osmotherapy. The research method used is a literature review from various sources which helps in reviewing the four focus of the study. Kidney is divided into three major parts, namely cortex, medulla, and renal pelvis. The excretory system has three stages, including filtration, reabsorption, and augmentation. Chronic kidney disease can be caused by age, where older age has a risk of developing CKD. Kidney disease can be divided into several stages, including normal, at risk of damage, kidneys are damaged, kidneys are not functioning properly and kidneys are no longer functioning or chronic kidney disease (CKD). The mechanism of action of Micro-Chinese Medicine Osmotherapy is divided based on its function, named as anti-inflammatory, anti-coagulation, and degradation. The results of treatment for kidney disease is Micro Medicine Osmotherapy could make all renal arteries of patients with chronic kidney disease widen and increase perfusion.
Karakterisasi Bacillus cereus strain IrN yang memiliki sifat multiresistensi terhadap tembaga dan pewarna Wahyu Irawati; Egilia Lucky; Yovanka P.P Tumakaka; Charles Immanuel; Eunike Bunga Marvella
Jurnal Biologi Udayana Vol 26 No 2 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i02.p10

Abstract

Peningkatan aktivitas industri tekstil semakin menambahkan jumlah limbah di lingkungan. Tembaga merupakan salah satu komponen pewarna sintetis sehingga limbah industri tekstil berpeluang mengandung pewarna dan tembaga. Bioremediasi menggunakan bakteri indigenus yang resisten terhadap pewarna dan tembaga diharapkan dapat meningkatkan efesiensi pengolahan limbah tekstil. Isolat bakteri IrN merupakan bakteri indigenus yang resisten terhadap tembaga dan diisolasi dari pengolahan limbah. Penelitian ini bertujuan untuk melakukan karakterisasi molekular serta menguji resistensi dan kemampuan dekolorisasi bakteri terhadap berbagai pewarna. Karakterisasi molekular dilakukan dengan analisis gen 16S rDNA. Medium yang digunakan adalah Luria Bertani dengan penambahan pewarna dan atau CuSO4 sebagai perlakuan. Pewarna yang digunakan ada 12 macam, yaitu: Methylene Blue, Malachite Green, Congo Red, Mordant Orange, Reactive Black, Direct Yellow, Basic Fuchsin, Reactive Orange, Disperse Orange, Remasol, Wantex Red, dan Wantex Yellow. Kemampuan dekolorisasi dan pertumbuhan bakteri diukur dengan menggunakan spektrofotometer. Hasil analisis 16S rDNA menunjukkan bahwa bakteri IrN teridentifikasi sebagai Bacillus cereus dengan kemiripan sebesar 99,91%. Isolat bakteri IrN memiliki multiresistensi terhadap 3 mM CuSO4 serta semua pewarna yang diuji pada konsentrasi 200 ppm, tetapi pada 500 ppm IrN hanya resisten terhadap methylene blue. Bakteri IrN dapat mendekolorisasi 200 ppm dan 500 ppm Methylene Blue masing-masing sebesar 42,78% dan 38,1%. Penambahan 3 mM CuSO4 pada medium 500 ppm Methylene BlueBlue tetap membuat bakteri mampu mendekolorisasi Methylene Blue, walaupun kemampuan dekolorisasinya menurun menjadi 34,17%. Isolat bakteri IrN juga dapat mendekolorisasi Malachite Green, Congo Red, Basic Fuchsine dengan kemampuan maksimal masing-masing sebesar 98,16%, 70,29%, dan 79,07%.
Peran Guru Kristen dalam Membentuk Pemahaman Siswa tentang Penciptaan Alam Semesta yang Bersumber pada Alkitab Fernando Christofer; Wahyu Irawati
Didache: Journal of Christian Education Vol 3, No 2 (2022): Desember 2022
Publisher : Sekolah Tinggi Teologi Simpson Ungaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46445/djce.v3i2.603

Abstract

The fall of man into sin has made man fail to understand God's truth about creation of the universe. Humans seek to uncover the truth of creation with secularism, which focuses on their thought and rejects God as creator. Worldview of creation that opposes God as Creator has become philosophical issues in Christian education that must be criticized with Biblical truth. Christian teachers have responsibility to shape students' worldview based on the Bible. This paper has purpose to examine Christian teachers’ role in shaping students' worldview of creation of the universe based on Biblical truth. The study was conducted with a study literature method that discusses three focuses named students’ worldview of creation of the universe, the role of Christian teachers in education, and the role of Christian teachers in shaping students' worldview. The worldview is formed from doctrine of Biblical creation. The role of the Christian teacher in shaping students' worldview is the responsibility of guiding and directing students learning orderliness of the universe which is by God’s work in Biblical truth. The next research suggested to carry out further analysis of students' perceptions and responses the understanding of creation as a form of evaluation of Christian teacher teaching practices.
PENGARUH PENERAPAN METODE INKUIRI UNTUK PENGUASAAN KONSEP PADA PEMBELAJARAN PAK JARAK JAUH Fernando Christofer; Wahyu Irawati
BONAFIDE: Jurnal Teologi dan Pendidikan Kristen Vol 3 No 2 (2022)
Publisher : SEKOLAH TINGGI TEOLOGI INJILI SETIA SIAU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46558/bonafide.v3i2.124

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Concepts comprehension is one of the important results for students. Low concept comprehension will make students get difficulty in problem-solving. The inquiry method is a means to improve 'students' conceptual understanding. The purpose of writing this practicum paper is to determine the efforts of improving 11th-grade 'students' conceptual understanding through the inquiry method in the distance learning context. The research using a descriptive qualitative method. It discusses five focuses of study, namely 'students' concept understanding in the learning process, the impact of inquiry methods in learning, the role of inquiry methods in improving students' conceptual understanding in distance learning, data analysis of 'students' with low understanding, and data analysis of increasing students' conceptual understanding through inquiry method. The research concluded that the inquiry method has succeeded-' in improving 11th-grade students’' conceptual understanding of distance learning. This success is supported by adequate technology advancements for teachers and students. The application of inquiry methods in improving students' conceptual understanding must be applied by Christian education purposes. Further research is suggested to analyze the effectiveness and success of inquiry method application by innovating strategies, media, or learning evaluation to improve the learning outcomes of students' conceptual understanding in the distance learning system.
12 TEXTILE DYES DECOLORIZATION BY COPPER-RESISTANT-BACTERIA KLEBSIELLA GRIMONTII, SHIGELLA FLEXNERI, ENTEROBACTER CLOACAE ISOLATED FROM CISADANE RIVER TANGERANG Wahyu Irawati; Vania Austine Callista Timotius; Ruben Patrick Adhiwijaya; Bellen Mouretta; Steven Anggawinata; Eunike Bunga Marvella
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 9 No. 2 (2023): February
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i2.7731

Abstract

Dyes and copper are dangerous contaminants because they are toxic. Bioremediation using indigenous bacteria is the best solution to overcome water pollution. Copper resistant bacteria usually have resistance to dyes thereby helping the bioremediation of dye and copper wastes. This study aims to examine the ability of indigenous bacteria isolated from the Cisadane River, namely Klebsiella grimontii IrCis3, Shigella flexneri IrCis5, Enterobacter cloacae IrCis6, and Enterobacter cloacae IrCis9 in terms of resistance and ability to decolorize 12 textile dyes namely methylene blue, malachite green, congo red, mordant orange, reactive black, direct yellow, basic fuchsin, reactive orange, dispersed orange, remasol red, wantex yellow and wantex red. The results showed that Shigella flexneri IrCis5, Enterobacter cloacae IrCis6, and Enterobacter cloacae IrCis9 were resistant to all dye concentrations of 200 and 500 ppm except Klebsiella grimontii IrCis3 did not grow on malachite green and basic fuchsin at concentrations of 200 ppm and methylene blue, malachite green and basic fuchsin concentration of 500 ppm. Only Shigella flexneri IrCis5 has the ability to decolorize 200 ppm basic fuchsin up to 87.23% after 3 days of incubation. The addition of 3 mM CuSO4 reduced the ability to decolorize Shigella flexneri IrCis5 to 0.57%.
The Potency of Vitamin C in Tomato Plant for the Result of Genetically Modified Lanceolate Gene Through Agrobacterium Tumefaciens Using CRISPR-CAS 9 Vannesa El Shaday Ruth Advenita; Christeven Mevotema; Iren Asima Situmorang; Lusiana Haris; Wahyu Irawati
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.4682

Abstract

Vitamin C is the important part in the formation of protein in the body, one of which is obtained from tomatoes (Solanum lycopersicum L.). Due to the needed of vitamin C, a way is needed to get vitamin C from tomatoes which are of higher value. Tomato genetically modified using the lanceolate gene through Agrobacterium tumefaciens using CRISPR-CAS9 is a promising solution for the food world. The literature study method is carried out by reviewing the theory and the results of previous research qualitatively. The results is that genetically engineered tomatoes have the advantage of high vitamin content by using plant improvement and modification. The bacterium Agrobacterium tumefaciens functions as a vector and insertion site for recombinant genes and CRISPR-CAS9 technology is used to modify the target genome because it has a CAS9 protein containing two homologous domains resulting in a new trait. Tomatoes with lanceolate leaves will have fruit with high vitamin content because the results of photosynthesis are focused on fruit development. Suggestions for conducting in-depth research to ensure safety in the food quality and health. The goals of the research were to 1) determine the potency of tomato rich in vitamin C using genetic engineering of Agrobacterium tumefaciens, 2) structure and function of the Lanceolate gene in plants, 3) application of CRISPR-CAS 9 in genetic engineering, and 4) advantages and disadvantages of genetically modified tomato. God gave humans the ability to manage His creation. So that humans can glorifying God through research.
Terapi Sel Punca sebagai Inovasi dalam Pengobatan Penyakit Autoimun untuk Mempertahankan Sistem Imunitas Tubuh Manusia Elysabeth Simatupang; Pahotkon Purba; Sherina Juliana; Yohana Ayustin; Wahyu Irawati
Jurnal Biologi Papua Vol 14, No 1 (2022)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.275 KB) | DOI: 10.31957/jbp.1513

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Autoimmune is a disease caused by imbalance of immunology and losing tolerance then causes autoimmunity to attack body tissues in organisms. This disease occurs when the self-antigens attached by adaptive immune response. This disease can be treated by stem cell therapy. Stem cell therapy is an assured alternative to autoimmune treatment. Stem cells that can differentiate or can change themselves into various cells according to the environment and function to replace the damaged body cells. This study aims to find out the utility of stem cell therapy as an innovation in the treatment of autoimmune diseases. Literasture review is the method used in this research and focus to discuss: 1) the body's immune system; 2) immune system disorders; 3) autoimmune disease mechanisms; and 4) Stem cell therapy as an alternative to autoimmune treatment. Through this paper, readers will know that there is one technology to use treating autoimmune diseases, stem cell therapy. The mechanism of utilisation of stem cell therapy varies with each type of autoimmune disease, but this therapy can also cause some side effects. Stem cell therapy repairs damaged cells and replaces them with new, more functional ones. Key words: autoimmune disease; stem cell therapy; human body; medication.
Multiresistensi dan Akumulasi Acinetobacter sp. IrC2 terhadap Logam Berat Wahyu Irawati; Aaron Hasthosaputro; Lucia Kusumawati
Jurnal Biologi Papua Vol 12, No 2 (2020)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (434.045 KB) | DOI: 10.31957/jbp.1207

Abstract

The increasing industrial activity in Indonesia, that is not equipped with appropriate waste treatment, has caused an increase of heavy metal contaminants in water bodies. Heavy metals contamination such as copper (Cu), mercury (Hg), cadmium (Cd), and lead (Pb) contamination in water bodies have endangered aquatic life and public health. For this reason, it is urgently important to lower down the concentration of heavy metal pollutants in the water bodies surrounding industrial areas. Compared to chemical remediation, bioremediation of heavy metal by using indigenous bacteria is more effective and economical, since it can be applied in situ directly and be used repeatedly. Acinetobacter sp. IrC2, used in this study, is Indonesian indigenous bacteria isolated from the industrial waste treatment facility in Rungkut, Surabaya. This study aims, firstly, to investigate the heavy metal multiresistance of Acinetobacter sp. IrC2 against mercury, cadmium, and lead. Secondly, this study intends to examine its bioaccumulation capacity for single and heavy metal alloys. The heavy metal multiresistance test was carried out by measuring the minimum heavy metal concentrations that inhibit bacterial growth (Minimum Inhibitory Concentration/MIC). The bioaccumulation capacity was measured using an atomic absorption spectrophotometer (AAS).  It is shown that Acinetobacter sp. IrC2 has high multiresistance to mercury, cadmium, and lead with MIC values of 12 mM, 8 mM, and 18 mM, respectively. Furthermore,  it is also resistant to  heavy metal mixture of 4.5 mM.  The mechanism of bacterial resistance in response to heavy metal toxicity, in general, is by accumulating heavy metals in the cells. The highest amount of accumulated heavy metals identified, from bacteria grown in the medium contains a mixture of heavy metals, were 0.023 mg, 0.084 mg, 0.684 mg, and 1.476 mg per gram of cell dry weight for copper, mercury, cadmium and lead respectively.  In conclusion, Acinetobacter sp. IrC2 is a promising heavy metal bioremediation agent due to its heavy metal multiresistance and accumulator characteristics.  Key words: Acinetobacter sp. IrC2; cadmium; copper; lead; merkuri
Peranan Intervensi Fisioterapi Metode Senam Kegel Untuk Mengatasi Kegagalan Kerja Sel Otot Polos Pada Kelainan Kandung Kemih Inkontinensia Urin Argorby Simanullang; Naomi Dwany; Ruth Y. Purba; Melany Djami; Wahyu Irawati
Jurnal Biologi Papua Vol 13, No 2 (2021)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.835 KB) | DOI: 10.31957/jbp.1501

Abstract

Urinary incotinensia is a disease that causes sufferers to experience urine without realizing it or is often referred to as bed-wetting. Urinary incotinence causes sufferers to experience difficulty or sleep disturbances, depression, isolation, lack of confidence, irritability, limiting social activities, and spending a lot of money on medication so that they really need a treatment solution. Urinary incontinence results from weakening of the smooth muscle in the bladder organs. One of the treatments that can be done to overcome urinary incontinence is using physiotherapy, namely by doing Kegel exercises. The purpose of this paper is to determine the role of physiotherapy interventions using Kegel exercises to overcome the failure of smooth muscle cells in urinary incontinence bladder disorders. This literature review will discuss four focus studies, namely: 1) the structure and function of the renal organ system, 2) the structure and working mechanism of smooth muscle cells, 3) cellular studies of urinary incontinence, and 4) treatment of urinary incontinence using physiotherapy interventions. Physiotherapy with Kegel exercises has the benefit of strengthening the work of the bladder sphincter and pelvic floor muscles. The mechanism is by increasing smooth muscle cell metabolism. The metabolic rate of the muscles will increase the strength of the smooth muscle and the nervous system to contract, so that the smooth muscle will get stronger. However, Kegel exercises do not completely cure urinary incontinence and only relieve the symptoms it causes.Key words: Physiotherapy; Urinary incontinence; Smooth muscles; Cells; Kegel exercises.
Co-Authors . PATRICIA Aaron Hasthosaputro ABYATAR HUGO BASKORO Alvionita Thana Ambarita, Polin P. Ambarita, Polin Parulian Andara Frida Sheilaliany Daely Angelica, Apriliana Anggun Anggun Aninditya Sri Nugraheni Argoby Simanullang Argorby Simanullang Ashiong P Munthe Ashiong Parheheon Munthe Bellen Mouretta Bermuli, Jessica Elfani Beta Mualiman Laoli Bora, Merliana Ina Charles Immanuel Chelvieana A. Christanti Christeven Mevotema Christian, Grace Purnamasari Christian, Salomo Christine Febriandini Tinambunan Clement Khrisman Laia Clement Khrisman Laia Damai Yanti Manalu David Dharmawan Artha David L Gultom Debora V. Widyanti Delfania Apang Madao Dellania Yvonne Harefa Desi L. Sihombing Destya Waty Silalahi Dipakalyano Dipakalyano Dipakalyano, Dipakalyano Djoko Prasetyo Dominica Feni Valentina Dr. Adolf J.N. Parhusip Dwi N. Susilowati Egilia Lucky Eklesia Taysa Orah Elisabeth Tita Ellen Nur Endah Pangesti Elysabeth Simatupang Eunike Bunga Marvella Eunike Bunga Marvella Eunike Bunga Marvella Fernando Christofer Fernando Christofer Fernando Corneles Moniharapon Fiska Ivana Pratami Putri Tokede Floreta D. Icasari Frichilya Intan Lawa Padang Gaol, Riris Lumban Georgine Gladis Paula Sulardi Geraldy John Meyners Gim Mi Kyong Golda Santiur Sianturi Grace Charity Mary Cartir Greisnaningsi Greisnaningsi HARI HARTIKO Helen Melenia Sianipar Herna Siahaan Hernawati Siahaan Hutauruk, Kristian I Made Samudra Ice Frans Kosi Imanuel Adhitya Wulanata, Imanuel Adhitya Intan Cidarbulan Matita Iren Asima Situmorang Jennie Agatherania Listira JOEDORO SOEDARSONO Josephine Elsa Diani Putranto Karnelasatri, . Keisha Hillary Pangalila Krisdarwindah Mardiana Kristin Armis Pasaribu Laia, Clement Khrisman Lastiar R. Sitompul Lastiar Roselyna Sitompul, Lastiar Roselyna Lucia Kusumawati Lusiana Haris Maharaja, Dwi P. Manalu, Damai Y. Manalu, Damai Yanti Marcellie, Jessica Marvella, Eunike Bunga Melany Djami Mia Parhusip Muhamad Ananda Munthe, Ashiong P. Munthe, Sri Wahyu Ningsih Nadya Aurelia Ratna PutrI Naomi Dwany Naomi Dwany Nida Sopiah Nida Sopiah Nida Sopiah Nommensen Pangihutan Ompusunggu Nommensen Pangihutan Ompusunggu Novera, Rusliani S. Pahotkon Purba Pangaribuan, Medeliene Christine Patricia Patricia Pebrianti Pebrianti Pingkan Imelda Wuisan Polin Parulian Ambarita Ponagadi, Merry Adisty Prasetyo, Djoko Purba, Friska Juliana Rahmawati, Sintia Y. Rahul Simon Situmeang Rani Nurlina Tifen Raudhatunnur Reinhard Pinontoan Reisky Megawati Tammu Reisky Megawati Tammu Reisky Megawati Tammu Reisky Tammu Resky Megawati Tammu Resti Nopriyanti Rido, Elisabeth M.T. Rosiva Betaria Purba Ruben Patrick Adhiwijaya Ruth Y. Purba Sabar Manampin Giawa Salomo Christian Shaday, Vannesa El Sherina Juliana Sherina Juliana Siane Indriani, Siane Siane Indriyani Silaban, Grace Niche Agnesa Silalahi, Ramses Silvana Putri Tarigan Simamora, Tanti Marsanda Sindy Friska Siringo-ringo, Sisilya Situmorang, Stefani Situmorang, Widya Srigika Natalia Boru Ginting Stephanus Jordan Jans Steven Anggawinata Sudarsono Siburian Suhartono, Andreas Valiant Sukri, Urbanus Susi Sulistia Susi Sulistia, Susi Tabitha Grace Larasati Tahya, Candra Tahya, Candra Yulius Takashi Reymond Rimpan Tammu, Reisky Tammu, Reisky Megawati Torreno, Jovan Nathanael Tri P. Priyatno Triwibowo Yuwono Vania Austine Callista Timotius Vannesa El Shaday Ruth Advenita Viona Heryani Arla Waruwu, A'azokhi Xaveria, Jessica Yadi Suryadi Yantra Wijaya Yeni Angelina Zega YENNY SORAYA Yesica C. Rhenata Yohana Ayustin Yohana Delvia Ayustin Yohanes Edi Gunanto Yovanka P.P Tumakaka Zebua, Satria Putri Aryani