Claim Missing Document
Check
Articles

Karakterisasi Bacillus cereus strain IrN yang memiliki sifat multiresistensi terhadap tembaga dan pewarna Wahyu Irawati; Egilia Lucky; Yovanka P.P Tumakaka; Charles Immanuel; Eunike Bunga Marvella
Jurnal Biologi Udayana Vol 26 No 2 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i02.p10

Abstract

Peningkatan aktivitas industri tekstil semakin menambahkan jumlah limbah di lingkungan. Tembaga merupakan salah satu komponen pewarna sintetis sehingga limbah industri tekstil berpeluang mengandung pewarna dan tembaga. Bioremediasi menggunakan bakteri indigenus yang resisten terhadap pewarna dan tembaga diharapkan dapat meningkatkan efesiensi pengolahan limbah tekstil. Isolat bakteri IrN merupakan bakteri indigenus yang resisten terhadap tembaga dan diisolasi dari pengolahan limbah. Penelitian ini bertujuan untuk melakukan karakterisasi molekular serta menguji resistensi dan kemampuan dekolorisasi bakteri terhadap berbagai pewarna. Karakterisasi molekular dilakukan dengan analisis gen 16S rDNA. Medium yang digunakan adalah Luria Bertani dengan penambahan pewarna dan atau CuSO4 sebagai perlakuan. Pewarna yang digunakan ada 12 macam, yaitu: Methylene Blue, Malachite Green, Congo Red, Mordant Orange, Reactive Black, Direct Yellow, Basic Fuchsin, Reactive Orange, Disperse Orange, Remasol, Wantex Red, dan Wantex Yellow. Kemampuan dekolorisasi dan pertumbuhan bakteri diukur dengan menggunakan spektrofotometer. Hasil analisis 16S rDNA menunjukkan bahwa bakteri IrN teridentifikasi sebagai Bacillus cereus dengan kemiripan sebesar 99,91%. Isolat bakteri IrN memiliki multiresistensi terhadap 3 mM CuSO4 serta semua pewarna yang diuji pada konsentrasi 200 ppm, tetapi pada 500 ppm IrN hanya resisten terhadap methylene blue. Bakteri IrN dapat mendekolorisasi 200 ppm dan 500 ppm Methylene Blue masing-masing sebesar 42,78% dan 38,1%. Penambahan 3 mM CuSO4 pada medium 500 ppm Methylene BlueBlue tetap membuat bakteri mampu mendekolorisasi Methylene Blue, walaupun kemampuan dekolorisasinya menurun menjadi 34,17%. Isolat bakteri IrN juga dapat mendekolorisasi Malachite Green, Congo Red, Basic Fuchsine dengan kemampuan maksimal masing-masing sebesar 98,16%, 70,29%, dan 79,07%.
Peran Guru Kristen dalam Membentuk Pemahaman Siswa tentang Penciptaan Alam Semesta yang Bersumber pada Alkitab Fernando Christofer; Wahyu Irawati
Didache: Journal of Christian Education Vol 3, No 2 (2022): Desember 2022
Publisher : Sekolah Tinggi Teologi Simpson Ungaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46445/djce.v3i2.603

Abstract

The fall of man into sin has made man fail to understand God's truth about creation of the universe. Humans seek to uncover the truth of creation with secularism, which focuses on their thought and rejects God as creator. Worldview of creation that opposes God as Creator has become philosophical issues in Christian education that must be criticized with Biblical truth. Christian teachers have responsibility to shape students' worldview based on the Bible. This paper has purpose to examine Christian teachers’ role in shaping students' worldview of creation of the universe based on Biblical truth. The study was conducted with a study literature method that discusses three focuses named students’ worldview of creation of the universe, the role of Christian teachers in education, and the role of Christian teachers in shaping students' worldview. The worldview is formed from doctrine of Biblical creation. The role of the Christian teacher in shaping students' worldview is the responsibility of guiding and directing students learning orderliness of the universe which is by God’s work in Biblical truth. The next research suggested to carry out further analysis of students' perceptions and responses the understanding of creation as a form of evaluation of Christian teacher teaching practices.
PENGARUH PENERAPAN METODE INKUIRI UNTUK PENGUASAAN KONSEP PADA PEMBELAJARAN PAK JARAK JAUH Fernando Christofer; Wahyu Irawati
BONAFIDE: Jurnal Teologi dan Pendidikan Kristen Vol 3 No 2 (2022)
Publisher : SEKOLAH TINGGI TEOLOGI INJILI SETIA SIAU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46558/bonafide.v3i2.124

Abstract

Concepts comprehension is one of the important results for students. Low concept comprehension will make students get difficulty in problem-solving. The inquiry method is a means to improve 'students' conceptual understanding. The purpose of writing this practicum paper is to determine the efforts of improving 11th-grade 'students' conceptual understanding through the inquiry method in the distance learning context. The research using a descriptive qualitative method. It discusses five focuses of study, namely 'students' concept understanding in the learning process, the impact of inquiry methods in learning, the role of inquiry methods in improving students' conceptual understanding in distance learning, data analysis of 'students' with low understanding, and data analysis of increasing students' conceptual understanding through inquiry method. The research concluded that the inquiry method has succeeded-' in improving 11th-grade students’' conceptual understanding of distance learning. This success is supported by adequate technology advancements for teachers and students. The application of inquiry methods in improving students' conceptual understanding must be applied by Christian education purposes. Further research is suggested to analyze the effectiveness and success of inquiry method application by innovating strategies, media, or learning evaluation to improve the learning outcomes of students' conceptual understanding in the distance learning system.
12 TEXTILE DYES DECOLORIZATION BY COPPER-RESISTANT-BACTERIA KLEBSIELLA GRIMONTII, SHIGELLA FLEXNERI, ENTEROBACTER CLOACAE ISOLATED FROM CISADANE RIVER TANGERANG Wahyu Irawati; Vania Austine Callista Timotius; Ruben Patrick Adhiwijaya; Bellen Mouretta; Steven Anggawinata; Eunike Bunga Marvella
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 9 No. 2 (2023): February
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i2.7731

Abstract

Dyes and copper are dangerous contaminants because they are toxic. Bioremediation using indigenous bacteria is the best solution to overcome water pollution. Copper resistant bacteria usually have resistance to dyes thereby helping the bioremediation of dye and copper wastes. This study aims to examine the ability of indigenous bacteria isolated from the Cisadane River, namely Klebsiella grimontii IrCis3, Shigella flexneri IrCis5, Enterobacter cloacae IrCis6, and Enterobacter cloacae IrCis9 in terms of resistance and ability to decolorize 12 textile dyes namely methylene blue, malachite green, congo red, mordant orange, reactive black, direct yellow, basic fuchsin, reactive orange, dispersed orange, remasol red, wantex yellow and wantex red. The results showed that Shigella flexneri IrCis5, Enterobacter cloacae IrCis6, and Enterobacter cloacae IrCis9 were resistant to all dye concentrations of 200 and 500 ppm except Klebsiella grimontii IrCis3 did not grow on malachite green and basic fuchsin at concentrations of 200 ppm and methylene blue, malachite green and basic fuchsin concentration of 500 ppm. Only Shigella flexneri IrCis5 has the ability to decolorize 200 ppm basic fuchsin up to 87.23% after 3 days of incubation. The addition of 3 mM CuSO4 reduced the ability to decolorize Shigella flexneri IrCis5 to 0.57%.
The Potency of Vitamin C in Tomato Plant for the Result of Genetically Modified Lanceolate Gene Through Agrobacterium Tumefaciens Using CRISPR-CAS 9 Vannesa El Shaday Ruth Advenita; Christeven Mevotema; Iren Asima Situmorang; Lusiana Haris; Wahyu Irawati
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.4682

Abstract

Vitamin C is the important part in the formation of protein in the body, one of which is obtained from tomatoes (Solanum lycopersicum L.). Due to the needed of vitamin C, a way is needed to get vitamin C from tomatoes which are of higher value. Tomato genetically modified using the lanceolate gene through Agrobacterium tumefaciens using CRISPR-CAS9 is a promising solution for the food world. The literature study method is carried out by reviewing the theory and the results of previous research qualitatively. The results is that genetically engineered tomatoes have the advantage of high vitamin content by using plant improvement and modification. The bacterium Agrobacterium tumefaciens functions as a vector and insertion site for recombinant genes and CRISPR-CAS9 technology is used to modify the target genome because it has a CAS9 protein containing two homologous domains resulting in a new trait. Tomatoes with lanceolate leaves will have fruit with high vitamin content because the results of photosynthesis are focused on fruit development. Suggestions for conducting in-depth research to ensure safety in the food quality and health. The goals of the research were to 1) determine the potency of tomato rich in vitamin C using genetic engineering of Agrobacterium tumefaciens, 2) structure and function of the Lanceolate gene in plants, 3) application of CRISPR-CAS 9 in genetic engineering, and 4) advantages and disadvantages of genetically modified tomato. God gave humans the ability to manage His creation. So that humans can glorifying God through research.
Terapi Sel Punca sebagai Inovasi dalam Pengobatan Penyakit Autoimun untuk Mempertahankan Sistem Imunitas Tubuh Manusia Elysabeth Simatupang; Pahotkon Purba; Sherina Juliana; Yohana Ayustin; Wahyu Irawati
Jurnal Biologi Papua Vol 14, No 1 (2022)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.275 KB) | DOI: 10.31957/jbp.1513

Abstract

Autoimmune is a disease caused by imbalance of immunology and losing tolerance then causes autoimmunity to attack body tissues in organisms. This disease occurs when the self-antigens attached by adaptive immune response. This disease can be treated by stem cell therapy. Stem cell therapy is an assured alternative to autoimmune treatment. Stem cells that can differentiate or can change themselves into various cells according to the environment and function to replace the damaged body cells. This study aims to find out the utility of stem cell therapy as an innovation in the treatment of autoimmune diseases. Literasture review is the method used in this research and focus to discuss: 1) the body's immune system; 2) immune system disorders; 3) autoimmune disease mechanisms; and 4) Stem cell therapy as an alternative to autoimmune treatment. Through this paper, readers will know that there is one technology to use treating autoimmune diseases, stem cell therapy. The mechanism of utilisation of stem cell therapy varies with each type of autoimmune disease, but this therapy can also cause some side effects. Stem cell therapy repairs damaged cells and replaces them with new, more functional ones. Key words: autoimmune disease; stem cell therapy; human body; medication.
Multiresistensi dan Akumulasi Acinetobacter sp. IrC2 terhadap Logam Berat Wahyu Irawati; Aaron Hasthosaputro; Lucia Kusumawati
Jurnal Biologi Papua Vol 12, No 2 (2020)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (434.045 KB) | DOI: 10.31957/jbp.1207

Abstract

The increasing industrial activity in Indonesia, that is not equipped with appropriate waste treatment, has caused an increase of heavy metal contaminants in water bodies. Heavy metals contamination such as copper (Cu), mercury (Hg), cadmium (Cd), and lead (Pb) contamination in water bodies have endangered aquatic life and public health. For this reason, it is urgently important to lower down the concentration of heavy metal pollutants in the water bodies surrounding industrial areas. Compared to chemical remediation, bioremediation of heavy metal by using indigenous bacteria is more effective and economical, since it can be applied in situ directly and be used repeatedly. Acinetobacter sp. IrC2, used in this study, is Indonesian indigenous bacteria isolated from the industrial waste treatment facility in Rungkut, Surabaya. This study aims, firstly, to investigate the heavy metal multiresistance of Acinetobacter sp. IrC2 against mercury, cadmium, and lead. Secondly, this study intends to examine its bioaccumulation capacity for single and heavy metal alloys. The heavy metal multiresistance test was carried out by measuring the minimum heavy metal concentrations that inhibit bacterial growth (Minimum Inhibitory Concentration/MIC). The bioaccumulation capacity was measured using an atomic absorption spectrophotometer (AAS).  It is shown that Acinetobacter sp. IrC2 has high multiresistance to mercury, cadmium, and lead with MIC values of 12 mM, 8 mM, and 18 mM, respectively. Furthermore,  it is also resistant to  heavy metal mixture of 4.5 mM.  The mechanism of bacterial resistance in response to heavy metal toxicity, in general, is by accumulating heavy metals in the cells. The highest amount of accumulated heavy metals identified, from bacteria grown in the medium contains a mixture of heavy metals, were 0.023 mg, 0.084 mg, 0.684 mg, and 1.476 mg per gram of cell dry weight for copper, mercury, cadmium and lead respectively.  In conclusion, Acinetobacter sp. IrC2 is a promising heavy metal bioremediation agent due to its heavy metal multiresistance and accumulator characteristics.  Key words: Acinetobacter sp. IrC2; cadmium; copper; lead; merkuri
Peranan Intervensi Fisioterapi Metode Senam Kegel Untuk Mengatasi Kegagalan Kerja Sel Otot Polos Pada Kelainan Kandung Kemih Inkontinensia Urin Argorby Simanullang; Naomi Dwany; Ruth Y. Purba; Melany Djami; Wahyu Irawati
Jurnal Biologi Papua Vol 13, No 2 (2021)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.835 KB) | DOI: 10.31957/jbp.1501

Abstract

Urinary incotinensia is a disease that causes sufferers to experience urine without realizing it or is often referred to as bed-wetting. Urinary incotinence causes sufferers to experience difficulty or sleep disturbances, depression, isolation, lack of confidence, irritability, limiting social activities, and spending a lot of money on medication so that they really need a treatment solution. Urinary incontinence results from weakening of the smooth muscle in the bladder organs. One of the treatments that can be done to overcome urinary incontinence is using physiotherapy, namely by doing Kegel exercises. The purpose of this paper is to determine the role of physiotherapy interventions using Kegel exercises to overcome the failure of smooth muscle cells in urinary incontinence bladder disorders. This literature review will discuss four focus studies, namely: 1) the structure and function of the renal organ system, 2) the structure and working mechanism of smooth muscle cells, 3) cellular studies of urinary incontinence, and 4) treatment of urinary incontinence using physiotherapy interventions. Physiotherapy with Kegel exercises has the benefit of strengthening the work of the bladder sphincter and pelvic floor muscles. The mechanism is by increasing smooth muscle cell metabolism. The metabolic rate of the muscles will increase the strength of the smooth muscle and the nervous system to contract, so that the smooth muscle will get stronger. However, Kegel exercises do not completely cure urinary incontinence and only relieve the symptoms it causes.Key words: Physiotherapy; Urinary incontinence; Smooth muscles; Cells; Kegel exercises.
Pelatihan Perencanaan Keuangan Keluarga Indonesia (PK2I) di GKAI Gading Serpong, Tangerang Imanuel Adhitya Wulanata Chrismastianto; Wahyu Irawati; Ashiong P. Munthe; Lastiar R. Sitompul; Kristin Armis Pasaribu; Damai Yanti Manalu
MITRA: Jurnal Pemberdayaan Masyarakat Vol. 7 No. 1 (2023): Mitra: Jurnal Pemberdayaan Masyarakat
Publisher : Institute for Research and Community Services

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/mitra.v7i1.3579

Abstract

The situation analysis in community service of Gereja Kristen Alkitab Indonesia (GKAI) Gading Serpong shows that their daily living costs allocation tends to be disproportionate because they spend more than they earn, causing them difficulties in planning the income they get every month. Furthermore, the disproportion results in economic problems and conflicts in the family. Therefore, PkM activities, through the implementation of PK2I, that can provide real solutions to address family economic problems are needed. It is hoped that the program can support the realization of a happy and prosperous family now and in the future. PK2I was conducted in form of face-to-face training with 20 participants, implementing health protocols to prevent Covid-19 in GKAI Gading Serpong, Tangerang. The implementation of PkM activities in the first session was about the explanation of financial planning concept and its importance for today's families. The session was then followed by PK2I simulations using simulation sheets. In this session, participants were accompanied by direct assistants. The recapitulation results of post-training evaluation form show that PkM activities in PK2I training have met the objectives and were able to provide significant benefits for all participants.
The Uniqueness of Endemic Animals Canary Crab (Birgus latro) as The Largest Crab Species in The Phylum Arthropoda Krisdarwindah Mardiana; Andara Frida Sheilaliany Daely; Wahyu Irawati
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.5029

Abstract

Birgus latro or walnut crab is the largest land arthropod animal in the world, this crab has a unique and complex anatomy and body morphology that supports its survival. The research objective was to describe the anatomy, morphology, habitat, behavior, role, nutrition, reproductive system, immune system, gas exchange system, blood circulation, digestive system, excretory system, and Birgus latro osmoregulation. The research method used is literature review. This type of research is a form of qualitative descriptive research with review techniques from research journals on Birgus latro in Indonesia. This research was conducted by analyzing the results of research that had been published nationally and internationally relating to the life of Birgus latro. Walnut crabs live in areas that are damp and dark and avoid the sun to reduce the occurrence of evaporation on their bodies. Hard body parts such as shells, claws, and skin contain lots of calcium and chitin for self- defense and climbing coconut trees. The circulatory system of the walnut crab is open, this crab is also an osmoregulatory animal. The advice that the author can give for the preservation of walnut crabs is to provide outreach to the community regarding the importance of maintaining and preserving the existence of walnut crabs which are almost extinct. Establishing a walnut shell conservation center must also be carried out by the government to maintain the coconut crab population, as well as cultivate walnut crabs with the right procedure.
Co-Authors . PATRICIA Aaron Hasthosaputro ABYATAR HUGO BASKORO Alvionita Thana Ambarita, Polin P. Ambarita, Polin Parulian Andara Frida Sheilaliany Daely Angelica, Apriliana Anggun Anggun Aninditya Sri Nugraheni Argoby Simanullang Argorby Simanullang Artha, David Dharmawan Ashiong P Munthe Ashiong Parheheon Munthe Ayustin, Yohana Delvia Bellen Mouretta Bermuli, Jessica Elfani Bora, Merliana Ina Charles Immanuel Chelvieana A. Christanti Christeven Mevotema Christian, Grace Purnamasari Christian, Salomo Clement Khrisman Laia Clement Khrisman Laia Daely, Andara Frida Sheilaliany Damai Yanti Manalu David L Gultom Debora V. Widyanti Delfania Apang Madao Desi L. Sihombing Destya Waty Silalahi, Destya Waty Dipakalyano Dipakalyano Dipakalyano, Dipakalyano Djoko Prasetyo Dominica Feni Valentina Dr. Adolf J.N. Parhusip Dwany, Naomi Dwi N. Susilowati Egilia Lucky Elisabeth Tita Ellen Nur Endah Pangesti Elysabeth Simatupang Eunike Bunga Marvella Eunike Bunga Marvella Eunike Bunga Marvella Fernando Christofer Fernando Christofer Fernando Corneles Moniharapon Fiska Ivana Pratami Putri Tokede Floreta D. Icasari Frichilya Intan Lawa Padang Gaol, Riris Lumban Georgine Gladis Paula Sulardi Gim Mi Kyong Giri, Mutiara Yaemeliza Venderry Grace Charity Mary Cartir Greisnaningsi Greisnaningsi Harefa, Dellania Yvonne HARI HARTIKO Helen Melenia Sianipar Herna Siahaan Hernawati Siahaan Hutauruk, Kristian I Made Samudra Imanuel Adhitya Wulanata, Imanuel Adhitya Intan Cidarbulan Matita Iren Asima Situmorang Jennie Agatherania Listira JOEDORO SOEDARSONO Josephine Elsa Diani Putranto Karnelasatri, . Keisha Hillary Pangalila Kosi, Ice Frans Krisdarwindah Mardiana Kristin Armis Pasaribu Laia , Clement Khrisman Laia, Clement Khrisman Laoli, Beta Mualiman Lastiar R. Sitompul Lastiar Roselyna Sitompul, Lastiar Roselyna Legesang, Eddowardho Otniel Lucia Kusumawati Lusiana Haris Maha, Paldevina Br Maharaja, Dwi P. Malau, Robeka Risky Manalu, Damai Y. Manalu, Damai Yanti Marcellie, Jessica Margaretha, Cecilia Marvella, Eunike Bunga Melany Djami Meyners, Geraldy John Mia Parhusip Muhamad Ananda Munthe, Ashiong P. Munthe, Sri Wahyu Ningsih Naomi Dwany Nexson Parhusip, Adolf Jan Nida Sopiah Nida Sopiah Nida Sopiah Nommensen Pangihutan Ompusunggu Nommensen Pangihutan Ompusunggu Novera, Rusliani S. Orah, Eklesia Taysa Pahotkon Purba Pangaribuan, Medeliene Christine Patricia Patricia Pebrianti Pebrianti, Pebrianti Pingkan Imelda Wuisan Polin Parulian Ambarita Ponagadi, Merry Adisty Prasetyo, Djoko Purba, Friska Juliana Purwanti, Ester Ayu Sri PutrI, Nadya Aurelia Ratna Rahmawati, Sintia Y. Rahul Simon Situmeang Rani Nurlina Tifen Raudhatunnur Reinhard Pinontoan Resky Megawati Tammu Resti Nopriyanti Rido, Elisabeth M.T. Rimpan, Takashi Reymond Rosiva Betaria Purba Ruben Patrick Adhiwijaya Ruth Y. Purba Sabar Manampin Giawa Salomo Christian Shaday, Vannesa El Sherina Juliana Sherina Juliana Siane Indriani, Siane Siane Indriyani Sianturi, Golda Santiur Silaban, Grace Niche Agnesa Silalahi, Ramses Silvana Putri Tarigan Simamora, Tanti Marsanda Sindy Friska Siringo-ringo, Sisilya Situmorang, Stefani Situmorang, Widya Srigika Natalia Boru Ginting Stephanus Jordan Jans Steven Anggawinata Sudarsono Siburian Suhartono, Andreas Valiant Sukri, Urbanus Susi Sulistia Susi Sulistia, Susi Tabitha Grace Larasati Tahya, Candra Tahya, Candra Yulius Tammu, Reisky Megawati Tinambunan , Christine Febriandini Torreno, Jovan Nathanael Tri P. Priyatno Triwibowo Yuwono Vania Austine Callista Timotius Vannesa El Shaday Ruth Advenita Viona Heryani Arla Waruwu, A'azokhi Xaveria, Jessica Yadi Suryadi Yantra Wijaya YENNY SORAYA Yesica C. Rhenata Yohana Ayustin Yohanes Edi Gunanto Yolanda, Shelomita Feby Yovanka P.P Tumakaka Zebua, Satria Putri Aryani Zega, Yeni Angelina