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Exploration and Identification of Trichoderma sp. with Baiting Method as a Biological Agent in Horticultural Lands Firdaus, Emilda Zahrotul; Indahsari, Wiwin Misnati Nur Indahsari; Bahri, Saiful; Tyastirin, Esti
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.386

The use of biological agents is an effective way to prevent the negative impacts of excessive synthetic chemical pesticide use. Trichoderma sp., a saprophytic fungus, acts as a biocontrol agent against various phytopathogenic molds and is more commonly found in rooted soil and leaves than in stems or fruits. This study aims to identify the characteristics and presence of Trichoderma sp. in horticultural land using the baiting method. Isolation was carried out by collecting fungi grown on rice, corn, and coconut bait using an inoculation needle. Three Trichoderma sp. isolates were obtained from chili and bamboo root soil using rice and corn bait. The results showed that the three isolates had similar characteristics: colonies were dark green, circular in shape, powdery in texture, and had entire margins. Microscopically, the isolates featured branched and upright conidiophores arranged vertically, short and thick phialides, round greenish conidia, and septate hyphae resembling threads.

Eco-enzyme from Orange Peel as a Key Ingredient in Anti Acne Peel-off Gel Mask Zummah, Atiqoh; Bahri, Saiful; Violando, Wiga Alif; Tyastirin, Esti
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.387

This study aims to develop a peel-off gel mask formulated from orange peel eco-enzyme solution and its physical characteristics and antibacterial activity against Staphylococcus epidermidis. The resulting gel mask has organoleptic characteristics of transparent brownish color, thick gel consistency, and fresh sour-sweet aroma. Physical characteristic tests showed that the gel mask meets pharmaceutical requirements, namely: pH value 4.5-6.5, spreadability 5-7 cm, and drying time 15-30 minutes. The mask showed good homogeneity without aggregates, although it was a little difficult to peel off, but it was not easily torn, and had stable thermodynamic stability. Antibacterial tests revealed that the peel-off gel mask made from orange peel eco-enzyme was effective in inhibiting the growth of Staphylococcus epidermidis. The diameters of the inhibition zones of the P1, P2, and P3 masks were 2.5 ± 0.5 mm, 2.1 ± 0.35 mm, and 1.5 ± 0.5 mm, respectively. These results indicate the potential of eco-enzymes from orange peel as active ingredients for peel-off gel mask formulation for skin care, especially in inhibiting acne-causing bacteria.

PENGARUH LAMA PERENDAMAN ZAT PENGATUR TUMBUH ALAMI AIR CUCIAN BERAS TERHADAP PERTUMBUHAN TANAMAN SAMBUNG NYAWA (Gynura procumbens) DENGAN METODE STEK BATANG Salsabilla Putri, Ambar; Tyastirin, Esti
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5625

This study aims to determine the effect of natural Plant Growth Regulators (PGR) from rice washing water on the growth of grafting plants through the stem cutting method. The research method used in this study was a Completely Randomized Design (CRD) consisting of 3 treatments and each treatment was repeated 8 times. The experiments carried out were as follows: P1 (control, without soaking in PGR rice washing water), P2 (soaking for 10 minutes in PGR rice washing water), and P3 (soaking for 20 minutes in PGR rice washing water). The parameters observed included plant height, number of leaves, and leaf area. The results showed that the treatment of soaking for 10 minutes in natural PGR rice washing water was the best treatment because the average value for the number of leaves tended to be high and affected the leaf area. The control treatment (without soaking) produced the highest plants. Soaking for 20 minutes (P3) tended to only increase the average growth value for each parameter each week.

PENGARUH MEDIA TANAM TERHADAP PERTUMBUHAN BAWANG MERAH (ALLIUM CEPA L.) MENGGUNAKAN HIDROPONIK SISTEM WICK Masaning Previanti, Melda; Tyastirin, Esti
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5626

Onion are one of the many types of shallots that exist in the world. Shallots are a member of the Allium genus which is usually used as a cooking spice. Demand for shallots will continue to increase along with people's needs that continue to increase. Hydroponics is a method of growing crops without using soil, usually using cocpeat, husks and sand as a substitute for soil. One hydroponic model that is easy to implement is the wick system method, where in this system the plants absorb water through the wick. The parameters observed were plant height, root length and number of tubers. The results of the research showed that the planting medium did not have a real influence on plant height and number of tubers, but on root length the planting medium had a real influence. The best planting medium for growing tall shallots is husk media: cocopeat (1:1) with the highest score of 27.90. Meanwhile, the root length and number of shallot bulbs were highest in the husk: sand (1:1) media with values of 16.00 and 6.22.

Produksi Jamur Entomopatogen Beauveria bassiana pada Limbah Pertanian dan Patogenisitasnya terhadap Ulat Grayak (Spodoptera litura) Novitasari, Dian; Rokhim, Saiku; Tyastirin, Esti; Faizah, Hanik
JRST (Jurnal Riset Sains dan Teknologi) Volume 9 No. 1 Maret 2025: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/jrst.v9i1.20678

Ulat grayak (Spodoptera litura) merupakan salah satu Organisme Pengganggu Tanaman (OPT) yang menyerang berbagai tanaman seperti kacang tanah, tembakau, kacang kedelai, kacang hijau, ubi jalar, bawang merah, cabai, dan jagung. Beauveria bassiana merupakan salah satu jamur entomopatogen yang memiliki kemampuan dalam mengendalikan serangga hama seperti S. litura. B. bassiana dikulturkan di media tumbuh dari limbah pertanian yang berupa molase tebu dan dedak padi dengan teknik kultur cair. Selanjutnya, biakan cair B. bassiana dari perlakuan media tumbuh diuji patogenisitasnya terhadap S. litura. Tujuan dari penelitian ini yaitu untuk mengetahui pengaruh media tumbuh pada sporulasi, daya kecambah, dan patogenisitas B. bassiana terhadap S. litura. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dan jenis penelitian eksperimental dengan 2 tahap penelitian. Tahap pertama yaitu menumbuhkan B. bassiana di beberapa perlakuan media tumbuh (molase tebu, dedak padi, dan Potato Dextrose Broth (PDB)). Tahap kedua yaitu B. bassiana (yang telah ditumbuhkan di beberapa perlakuan media tumbuh) diuji patogenisitasnya pada S. litura tahap larva instar III. Hasil penelitian menunjukkan bahwa sporulasi tertinggi terdapat pada kultur B. bassiana di perlakuan media PDB (21,417 × 109 spora/ml), diikuti dengan perlakuan media dedak padi 13% (6,058 × 109 spora/ml). Sedangkan, daya kecambah spora tertinggi terdapat pada kultur B. bassiana di perlakuan media dedak padi 13%, yaitu 57,54%. Kultur B. bassiana di perlakuan media dedak padi 13% mengakibatkan mortalitas larva instar III S. litura tertinggi, yaitu 53,33%.

PENGARUH LAMA PERENDAMAN ZAT PENGATUR TUMBUH ALAMI AIR CUCIAN BERAS TERHADAP PERTUMBUHAN TANAMAN SAMBUNG NYAWA (Gynura procumbens) DENGAN METODE STEK BATANG Salsabilla Putri, Ambar; Tyastirin, Esti
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5625

This study aims to determine the effect of natural Plant Growth Regulators (PGR) from rice washing water on the growth of grafting plants through the stem cutting method. The research method used in this study was a Completely Randomized Design (CRD) consisting of 3 treatments and each treatment was repeated 8 times. The experiments carried out were as follows: P1 (control, without soaking in PGR rice washing water), P2 (soaking for 10 minutes in PGR rice washing water), and P3 (soaking for 20 minutes in PGR rice washing water). The parameters observed included plant height, number of leaves, and leaf area. The results showed that the treatment of soaking for 10 minutes in natural PGR rice washing water was the best treatment because the average value for the number of leaves tended to be high and affected the leaf area. The control treatment (without soaking) produced the highest plants. Soaking for 20 minutes (P3) tended to only increase the average growth value for each parameter each week.

PENGARUH MEDIA TANAM TERHADAP PERTUMBUHAN BAWANG MERAH (ALLIUM CEPA L.) MENGGUNAKAN HIDROPONIK SISTEM WICK Masaning Previanti, Melda; Tyastirin, Esti
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5626

Onion are one of the many types of shallots that exist in the world. Shallots are a member of the Allium genus which is usually used as a cooking spice. Demand for shallots will continue to increase along with people's needs that continue to increase. Hydroponics is a method of growing crops without using soil, usually using cocpeat, husks and sand as a substitute for soil. One hydroponic model that is easy to implement is the wick system method, where in this system the plants absorb water through the wick. The parameters observed were plant height, root length and number of tubers. The results of the research showed that the planting medium did not have a real influence on plant height and number of tubers, but on root length the planting medium had a real influence. The best planting medium for growing tall shallots is husk media: cocopeat (1:1) with the highest score of 27.90. Meanwhile, the root length and number of shallot bulbs were highest in the husk: sand (1:1) media with values of 16.00 and 6.22.

Pengaruh Penggunaan Probiotik Terhadap Kualitas Air Kolam Budidaya Dan Pertumbuhan Ikan Lele Sangkuriang (Clarias gariepinus) Maziyan, Intan Nabila; Zumar, Muhammad Rifqi; Bahri, Saiful; Tyastirin, Esti
Jurnal Penyuluhan Perikanan dan Kelautan Vol 19, No 1 (2025)
Publisher : Program Studi Penyuluhan Perikanan Politeknik Ahli Usaha Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33378/jppik.v19i1.531

Ikan lele sangkuriang (Clarias gariepinus) merupakan jenis ikan air tawar yang banyak dibudidaya di Indonesia karna memiliki keunggulan harga yang relatif murah, gizi tinggi dan pertumbuhan cepat. Kualitas air kolam budidaya sangat mempengaruhi keberhasilan budidaya ikan. Faktor-faktor yang mempengaruhi kualitas air kolam budidaya. ialah pH, suhu, dan amonia. Semakin tinggi kadar bahan organik di dalamnya maka kualitas air kolam akan semakin menurun. Salah satu usaha untuk meningkatkan kualitas air adalah menggunakan probiotik. Tujuan penelitian ini adalah mengetahui pengaruh penggunaan probiotik terhadap kualitas air dan pertumbuhan ikan lele sangkuriang pada media budidaya. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) dengan satu faktor perlakuan, yaitu penambahan probiotik yakni probitik nitrobacter Yu Kaji Pondok Pesantren Baitus Surur Mojokerto, probiotik buatan sendiri, dan probiotik EM4, dan kontrol dengan 4 perlakuan dan 3 kali ulangan dengan jumlah ikan sebanyak 10 ekor pada masing-masing kolam. Pengambilan data dilakukan setiap 2 minggu sekali selama 12 minggu. Data yang diamati meliputi berat, panjang, kelangsungan hidup dan parameter fisik lingkungan. Perlakuan yang tidak memenuhi SNI ada pada kolam kontrol dengan kadar pH 6,4 dan kolam probiotik yu kaji dengan kadar amonia 1,8 ppm. Nilai kelangsungan hidup ikan lele paling tinggi berada pada perlakuan probiotik buatan sendiri yaitu sebesar 83,33%. Nilai rerata pertumbuhan berat ikan lele paling baik dan seragam berdasarkan standar deviasi ada pada perlakuan probiotik buatan sendiri yaitu 60,36 gram + 4,91, sedangkan nilai rerata pertumbuhan + standar deviasi panjang ikan lele paling ada pada perlakuan probiotik buatan sendiri yaitu 18,74 cm + 0,24.

IDENTIFIKASI SENYAWA METABOLIT SEKUNDER PADA EKSTRAK METHANOL BATANG KEMANGI (Ocimum bacilicum L): IDENTIFICATION OF SECONDARY METABOLYTE IN METHANOL EXTRACT OF BASIL STEMS (Ocimum bacilicum L) Andiarna, Funsu; Kumalasari, Mei Lina Fitri; Tyastirin, Esti; Pribadi, Eko Teguh; Khoiriyah, Romyun Alvy; Oktorina, Sarita
GEMA KESEHATAN Vol. 15 No. 2 (2023): Desember 2023
Publisher : POLTEKKES KEMENKES JAYAPURA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47539/gk.v15i2.420

Tanaman kemangi (Ocimum bacilicum L) adalah salah satu tanaman obat tradisional yang paling banyak dikembangkan di negara Indonesia. Tanaman kemangi memiliki banyak manfaat seperti mengobati demam, diare, batuk, pusing, mual, dan lain sebagainya. Tujuan dari penelitian ini untuk mengidentifikasi senyawa metabolit sekunder pada batang tanaman kemangi. Ekstrak kemangi menggunakan pelarut metanol 96%. Batang daun kemangi dilakukan proses penyarian dengan metode maserasi. Ekstrak kemangi selanjutnya dilakukan uji fitokimia untuk mengetahui kandungan senyawa metabolit sekunder. Hasil dari penelitian ini menunjukkan bahwa pada batang tanaman kemangi terdapat kandungan senyawa metabolit sekunder yaitu flavonoid, tannin, alkaloid dan saponin. Hal ini dapat menjelaskan bahwa ekstrak kemangi memiliki potensi yang kuat dalam aktivitas antioksidan yang memiliki peran penting dalam pemanfaatan obat tradisional. Senyawa flavonoid paling umum dijadikan sebagai antioksidan. Selain itu beberapa jenis senyawa alkaloid, tanin dan saponin juga mempunyai efek antioksidan. Kata Kunci: Kemangi, Metanol, Ocimum bacilicum L, Senyawa metabolit sekunder The basil plant (Ocimum bacilicum L) is one of Indonesia's most developed traditional medicinal plants. Basil plants have many benefits, such as treating fever, diarrhea, cough, dizziness, nausea, etc. The study aims to identify secondary metabolite compounds in the stem of basil plants. Basil extract uses 96% methanol solvent. Stems of basil leaves carried out the process of expulsion by maceration method. The phytochemical test was conducted through basil extraction to determine the secondary content of metabolite compounds. The results of this research show that the stem of the basil plant is a secondary metabolite compound, namely flavonoids, alkaloids, tannins, and saponins. It can explain that basil extract has a strong potential for antioxidant activity that has an essential role in traditional medicine. Keywords: Methanol, Ocimum bacilicum, Secondary metabolites compounds, Stem basil

Prebiotic Potential of Gembili, Dahlia, and Yacon Tuber Flours Tyastirin, Esti; Oktorina, Sarita; Febriyanti, Ikke Arina
Journal of Health Science and Prevention Vol. 8 No. 2 (2024): JHSP Vol 8 No 2 – 2024
Publisher : State Islamic University of Sunan Ampel

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/jhsp.v8i2.1259

Inulin is a type of prebiotic which is generally produced from artichoke plants (Helianthus tuberosus). Considering the diversity of tubers in Indonesia, in this study the prebiotic potential of inulin was tested from various tubers, namely gembili tubers (Dioscororea esculenta), dahlia tubers (Dahlia Pinnata L.) and yakon tubers (Smallanthus sonchifolius). In this study, the prebiotic activity of tubers was tested against bifidiobacterium bacteria. Bacterial growth was carried out on MRS media, which is added with inulin from each tuber as much as 1% b/v gram. In addition to that negative control group and positive control group (comercial inulin) were added. During the incubation time of 24 hours, 48 and 92 hours, all varian group has positive correlation with the growth of Bifidiobacterium. The bigger correlation was found in Gembili tuber group with R = 0,9 and had the maxiumun bifidiobacterium colony which reached 14.4 x 108 CFU/ml.

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