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Proses dan Kendala Pembelajaran Biologi di Masa Pandemi Covid-19: Analisis Respon Mahasiswa Jariyah, Ita Ainun; Tyastirin, Esti
Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika Vol. 4 No. 2: July 2020
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/e-saintika.v4i2.224

Program studi Biologi UIN Sunan Ampel Surabaya telah memberlakukan pembelajaran daring pada Semester Genap Tahun Akademik 2019/2020 sebagai upaya memastikan perkuliahan tetap berjalan di masa pandemi COVID-19. Penelitian ini bertujuan untuk menganalisis proses dan kendala pembelajaran biologi di masa pandemi COVID-19 berdasarkan respon mahasiswa Jenis Penelitian ini adalah deskriptif kualitatif, Pengambilan data dilakukan dengan membagikan Google form yang berisi 11 pertayaan kepada 82 orang mahasiswa angakatan 2017, 2018, dan 2019. Hasil penelitian ini menunjukkan bahwa proses perkuliahan daring di Prodi Biologi UIN Sunan Ampel Surabaya berjalan cukup baik. Beberapa kendala diantaranya banyak menghabiskan kuota serta jaringan internet lemah. Proses perkuliahan masih perlu untuk terus dioptimalkan dan perlu untuk dilakukan evaluasi demi perbaikan perkuliahan daring pada periode berikutnya.The Biology Learning Processes and Constraints in the Covid-19 Pandemic Period: Analysis of Student ResponsesAbstractThe Biology Study Program of UIN Sunan Ampel Surabaya has implemented online learning in the Even Semester, Academic Year 2019/2020 as an effort to ensure lectures continue during the COVID-19 pandemic. This study aimed to analyze the processes and constraints of biology learning in the COVID-19 pandemic based on student responses. This research type was qualitative descriptive, data collection was done by distributing Google form containing 11 questions to 82 students 2017, 2018, and 2019. The results of this study indicate that the online lecture process at Biology Study Program of UIN Sunan Ampel Surabaya was going pretty well. Some obstacles include spending a lot of quota and a weak internet network. The lecture process still needs to be continuously optimized and evaluation needs to be done to improve online lectures in the next period.

Analisis Perbandingan Perilaku Hewan Kucing Liar (Felis silvestris) Jantan Sebelum Di Kastrasi Dan Pasca Di Kastrasi Andini Nurvania Putri; Esti Tyastirin
MULTIPLE: Journal of Global and Multidisciplinary Vol. 2 No. 9 (2024): September
Publisher : Institute of Educational, Research, and Community Service

Show Abstract | Download Original | Original Source | Check in Google Scholar

Di Indonesia jenis kucing yang paling dominan adalah kucing liar (Felis silvestris). Berdasarkan survei Rakuten Insight Global presentase kepemilikan Felis silvestris menyentuh 47% dari seluruh responden Indonesia. Hal ini membuktikan bahwa populasi kucing di Indonesia banyak terutama kucing berjenis kelamin jantan. Salah satu Upaya mengendalikan populasi kucing adalah dengan kastrasi, namun Upaya tersebut memiliki efek samping. Tujuan dari penelitian ini yaitu untuk mengetahui perbandingan perilaku dari kucing liar (Felis silvestris) jantan yang dikastrasi dan belum dikastrasi. Metode yang digunakan pada pengamatan ini yaitu metode observasi secara langsung terkait perilaku kucing liar, yang meliputi perilaku ingestif,seks, interaksi dan agonistic. Sampel pengamatan adalah 4 kucing jantan 2 diantaranya belum dikastrasi dan 2 lainnya sudah dikastrasi. Hasil pengamatan yang diperoleh yaitu terdapat perbedaan beberapa jenis pola perilaku yang ditunjukkan oleh kucing jantan yang sudah di kastrasi maupun yang belum dikastrasi yakni perbedaan pada perilaku ingestif, perilaku seks, perilaku interaksi dan perilaku agonistic. Pola tersebut berhubungan dengan beberapa hipotesis berupa kemampuan berburu makan, kawin, berinteraksi ke manusia dan mengeong serta berkelahi. Setiap pola-pola perilaku tersebut disebabkan oleh beberapa faktor tertentu, yaitu faktor fisiologis akibat kastrasi dan lingkungan habitatnya.

Optimization of Various ITS rDNA Amplification Protocol of Yeast Isolated from Giant Honey Beehives (Apis dorsata) Choiriyah, S.Si, Chumaidatul; Firdhausi, Nirmala Fitria; Tyastirin, Esti; Rachmawati, Yuanita; Hadi, Moch. Irfan
Jurnal Riset Biologi dan Aplikasinya Vol. 3 No. 2 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n2.p80-87

Indonesia is a country with high variability of microorganisms, including bacteria, yeast, and fungi. Yeast isolates could be isolated from the honeycomb of Apis dorsata. Molecular approaches were used to identify yeast using ribosomal DNA gene sequences, called the ITS gene. The optimum condition for DNA extractions and amplifications are needed for the successfully of molecular identification. Therefore, it is necessary to optimize the DNA extraction and amplification of several protocols to obtain good identification results. This study aimed to compare the effects of DNA extraction with various temperatures and different amplification protocols. LIPI reference DNA extraction protocol with the boiling method and variations in incubation time of 10, 15, and 20 minutes at a temperature of 98° C. Meanwhile, for the amplification of yeast DNA using a variety of different amplification protocols. The results showed the optimal time of incubation was 10 minutes in K1 isolates with DNA purity of 1.896. meanwhile, for isolates K2, K3, and K4 each with a purity of 2.246, 2.335, and 1.748. optimal DNA amplification results were indicated by the presense of DNA bands for each sample K1, K2, K3, and K4, namely 503, 542, 492, and 526 bp. In this study, it can be concluded that the optimal incubation time for the extraction process is 10 minutes. In addition, the optimal amplification protocol was shown in the DNA bands in all sample.

Exploration and Identification of Trichoderma sp. with Baiting Method as a Biological Agent in Horticultural Lands Firdaus, Emilda Zahrotul; Indahsari, Wiwin Misnati Nur Indahsari; Bahri, Saiful; Tyastirin, Esti
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.386

The use of biological agents is an effective way to prevent the negative impacts of excessive synthetic chemical pesticide use. Trichoderma sp., a saprophytic fungus, acts as a biocontrol agent against various phytopathogenic molds and is more commonly found in rooted soil and leaves than in stems or fruits. This study aims to identify the characteristics and presence of Trichoderma sp. in horticultural land using the baiting method. Isolation was carried out by collecting fungi grown on rice, corn, and coconut bait using an inoculation needle. Three Trichoderma sp. isolates were obtained from chili and bamboo root soil using rice and corn bait. The results showed that the three isolates had similar characteristics: colonies were dark green, circular in shape, powdery in texture, and had entire margins. Microscopically, the isolates featured branched and upright conidiophores arranged vertically, short and thick phialides, round greenish conidia, and septate hyphae resembling threads.

Eco-enzyme from Orange Peel as a Key Ingredient in Anti Acne Peel-off Gel Mask Zummah, Atiqoh; Bahri, Saiful; Violando, Wiga Alif; Tyastirin, Esti
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.387

This study aims to develop a peel-off gel mask formulated from orange peel eco-enzyme solution and its physical characteristics and antibacterial activity against Staphylococcus epidermidis. The resulting gel mask has organoleptic characteristics of transparent brownish color, thick gel consistency, and fresh sour-sweet aroma. Physical characteristic tests showed that the gel mask meets pharmaceutical requirements, namely: pH value 4.5-6.5, spreadability 5-7 cm, and drying time 15-30 minutes. The mask showed good homogeneity without aggregates, although it was a little difficult to peel off, but it was not easily torn, and had stable thermodynamic stability. Antibacterial tests revealed that the peel-off gel mask made from orange peel eco-enzyme was effective in inhibiting the growth of Staphylococcus epidermidis. The diameters of the inhibition zones of the P1, P2, and P3 masks were 2.5 ± 0.5 mm, 2.1 ± 0.35 mm, and 1.5 ± 0.5 mm, respectively. These results indicate the potential of eco-enzymes from orange peel as active ingredients for peel-off gel mask formulation for skin care, especially in inhibiting acne-causing bacteria.

PENGARUH LAMA PERENDAMAN ZAT PENGATUR TUMBUH ALAMI AIR CUCIAN BERAS TERHADAP PERTUMBUHAN TANAMAN SAMBUNG NYAWA (Gynura procumbens) DENGAN METODE STEK BATANG Salsabilla Putri, Ambar; Tyastirin, Esti
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5625

This study aims to determine the effect of natural Plant Growth Regulators (PGR) from rice washing water on the growth of grafting plants through the stem cutting method. The research method used in this study was a Completely Randomized Design (CRD) consisting of 3 treatments and each treatment was repeated 8 times. The experiments carried out were as follows: P1 (control, without soaking in PGR rice washing water), P2 (soaking for 10 minutes in PGR rice washing water), and P3 (soaking for 20 minutes in PGR rice washing water). The parameters observed included plant height, number of leaves, and leaf area. The results showed that the treatment of soaking for 10 minutes in natural PGR rice washing water was the best treatment because the average value for the number of leaves tended to be high and affected the leaf area. The control treatment (without soaking) produced the highest plants. Soaking for 20 minutes (P3) tended to only increase the average growth value for each parameter each week.

PENGARUH MEDIA TANAM TERHADAP PERTUMBUHAN BAWANG MERAH (ALLIUM CEPA L.) MENGGUNAKAN HIDROPONIK SISTEM WICK Masaning Previanti, Melda; Tyastirin, Esti
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5626

Onion are one of the many types of shallots that exist in the world. Shallots are a member of the Allium genus which is usually used as a cooking spice. Demand for shallots will continue to increase along with people's needs that continue to increase. Hydroponics is a method of growing crops without using soil, usually using cocpeat, husks and sand as a substitute for soil. One hydroponic model that is easy to implement is the wick system method, where in this system the plants absorb water through the wick. The parameters observed were plant height, root length and number of tubers. The results of the research showed that the planting medium did not have a real influence on plant height and number of tubers, but on root length the planting medium had a real influence. The best planting medium for growing tall shallots is husk media: cocopeat (1:1) with the highest score of 27.90. Meanwhile, the root length and number of shallot bulbs were highest in the husk: sand (1:1) media with values of 16.00 and 6.22.

Produksi Jamur Entomopatogen Beauveria bassiana pada Limbah Pertanian dan Patogenisitasnya terhadap Ulat Grayak (Spodoptera litura) Novitasari, Dian; Rokhim, Saiku; Tyastirin, Esti; Faizah, Hanik
JRST (Jurnal Riset Sains dan Teknologi) Volume 9 No. 1 Maret 2025: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/jrst.v9i1.20678

Ulat grayak (Spodoptera litura) merupakan salah satu Organisme Pengganggu Tanaman (OPT) yang menyerang berbagai tanaman seperti kacang tanah, tembakau, kacang kedelai, kacang hijau, ubi jalar, bawang merah, cabai, dan jagung. Beauveria bassiana merupakan salah satu jamur entomopatogen yang memiliki kemampuan dalam mengendalikan serangga hama seperti S. litura. B. bassiana dikulturkan di media tumbuh dari limbah pertanian yang berupa molase tebu dan dedak padi dengan teknik kultur cair. Selanjutnya, biakan cair B. bassiana dari perlakuan media tumbuh diuji patogenisitasnya terhadap S. litura. Tujuan dari penelitian ini yaitu untuk mengetahui pengaruh media tumbuh pada sporulasi, daya kecambah, dan patogenisitas B. bassiana terhadap S. litura. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dan jenis penelitian eksperimental dengan 2 tahap penelitian. Tahap pertama yaitu menumbuhkan B. bassiana di beberapa perlakuan media tumbuh (molase tebu, dedak padi, dan Potato Dextrose Broth (PDB)). Tahap kedua yaitu B. bassiana (yang telah ditumbuhkan di beberapa perlakuan media tumbuh) diuji patogenisitasnya pada S. litura tahap larva instar III. Hasil penelitian menunjukkan bahwa sporulasi tertinggi terdapat pada kultur B. bassiana di perlakuan media PDB (21,417 × 109 spora/ml), diikuti dengan perlakuan media dedak padi 13% (6,058 × 109 spora/ml). Sedangkan, daya kecambah spora tertinggi terdapat pada kultur B. bassiana di perlakuan media dedak padi 13%, yaitu 57,54%. Kultur B. bassiana di perlakuan media dedak padi 13% mengakibatkan mortalitas larva instar III S. litura tertinggi, yaitu 53,33%.

PENGARUH LAMA PERENDAMAN ZAT PENGATUR TUMBUH ALAMI AIR CUCIAN BERAS TERHADAP PERTUMBUHAN TANAMAN SAMBUNG NYAWA (Gynura procumbens) DENGAN METODE STEK BATANG Salsabilla Putri, Ambar; Tyastirin, Esti
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5625

This study aims to determine the effect of natural Plant Growth Regulators (PGR) from rice washing water on the growth of grafting plants through the stem cutting method. The research method used in this study was a Completely Randomized Design (CRD) consisting of 3 treatments and each treatment was repeated 8 times. The experiments carried out were as follows: P1 (control, without soaking in PGR rice washing water), P2 (soaking for 10 minutes in PGR rice washing water), and P3 (soaking for 20 minutes in PGR rice washing water). The parameters observed included plant height, number of leaves, and leaf area. The results showed that the treatment of soaking for 10 minutes in natural PGR rice washing water was the best treatment because the average value for the number of leaves tended to be high and affected the leaf area. The control treatment (without soaking) produced the highest plants. Soaking for 20 minutes (P3) tended to only increase the average growth value for each parameter each week.

PENGARUH MEDIA TANAM TERHADAP PERTUMBUHAN BAWANG MERAH (ALLIUM CEPA L.) MENGGUNAKAN HIDROPONIK SISTEM WICK Masaning Previanti, Melda; Tyastirin, Esti
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5626

Onion are one of the many types of shallots that exist in the world. Shallots are a member of the Allium genus which is usually used as a cooking spice. Demand for shallots will continue to increase along with people's needs that continue to increase. Hydroponics is a method of growing crops without using soil, usually using cocpeat, husks and sand as a substitute for soil. One hydroponic model that is easy to implement is the wick system method, where in this system the plants absorb water through the wick. The parameters observed were plant height, root length and number of tubers. The results of the research showed that the planting medium did not have a real influence on plant height and number of tubers, but on root length the planting medium had a real influence. The best planting medium for growing tall shallots is husk media: cocopeat (1:1) with the highest score of 27.90. Meanwhile, the root length and number of shallot bulbs were highest in the husk: sand (1:1) media with values of 16.00 and 6.22.

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