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Maheno Sri Widodo, Maheno Sri
Program Studi Budidaya Perairan - Fakultas Perikanan Dan Ilmu Kelautan Universitas Brawijaya
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Journal : Journal of Tropical Diversity

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Identifikasi Genetik Ikan Nilem dengan Pendekatan DNA Barcoding Dailami, Muhammad; Yuniarti, Ating; Widodo, Maheno Sri; Pratama, Rakhmad Ndaru; Ariyani, Destia Fitri; Siahaan, Marthin Daniel Theo Gratia; Maulana, Antajala Rian; Ramadhan, Surya Dewa; Putri, Radwa Fathin H; Nurcahyani, Shinta Eka; Milansari, Sindi; Mayor, Corazon Ell Merilia Papuani
Journal of Tropical Diversity Vol 1 No 1 (2025): Februari
Publisher : Penerbit Brainy Bee

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.64283/jotdiverse.2025.1(1):3

Abstract

Nilem fish (Osteochilus vittatus) is a species of freshwater fish from the Cyprinidae family which has ecological and economic importance in Indonesia, with its natural habitat in rivers, swamps and stagnant waters. Accurate species identification is very important in biological research and fisheries resource management, but morphological identification methods have several limitations. DNA barcoding is a revolutionary molecular technique that uses short DNA sequences to identify and differentiate species with high accuracy. The use of DNA barcoding in identifying freshwater fish in Indonesia has shown significant development, including in a comprehensive study documenting 1,172 native freshwater fish species. The research "Genetic Identification of Nilem Fish using the DNA Barcoding Approach" aims to characterize the genetics of nilem fish, compare the genetic profiles of various populations, and evaluate the effectiveness of DNA barcoding in distinguishing nilem fish from other Osteochilus species, with the hope of contributing to fish conservation and sustainable management efforts nilem in Indonesia. The DNA sequencing results obtained a sequence with a length of 619 base pairs with the composition of Nucleotides A (27.2%), G (17.6%), C (26.4%), T (28.8%). The BLAST results with the NCBI database showed a similarity of 99.84% with the O. vittatus sequence and 99.83% with O. hasseltii. Both species are synonyms with the official name O. vittatus. Phylogenetic analysis is also in line with the BLAST results which show that the sample sequence is in the same clade as the O. vittatus and O. hasseltii sequences. Identification with molecular markers of the COI gene was successfully used in determining the species of the Nilem fish (O. vittatus).
Co-Authors Abdul Rahem Faqih Agoes Soeprijanto, Agoes Aida Sartimbul Aida Sartimbul Aida Sartimbul Amalia Ayuk Riyadini Amalia, Elok Amanullah, Muhammad Azmi Andi Masriah Andik Isdianto Andra Rejekineng Rahayu Rahayu Andra Rejekining Rahayu Angga Wira Perdana Anik Martinah Hariati Apriliyanti, Fisma Josara Ariyani, Destia Fitri Arning Wilujeng Ekawati Ating Yuniarti Bayu Kusuma Bayu Kusuma Dedi Pardiansyah Dewa Gede Raka Wiadnya Dewi Susylowati Diana Arfiati Eka Fitria Febriani Emyliana Listiowati, Emyliana Eric Armando Fani Fariedah Faqih, Abd. Raheem Faqih, Abdul Raheem Fariedah, Fani Fitri Sil Valen Frits Jamlaay, Frits Handoko, Lugu Tri Harahap, Muhammad Alfiandi Rachmad Harsuko Riniwati Harun Wijaya I Nengah Gde Sugestya Ihsan, Gusti Abdillah Ir. Mulyanto, MS Ir. Mulyanto, MS, Ir. Mulyanto, Irfanov Hafiz Kholil, Kiki Nur Azam Kurniasari, Reni Dyah Yuni Kusuma, Baruna Kusuma, Bayu Kusuma, Wahyu Endra Lestari, Bela Fatma Hani Ayu Lugu Tri Handoko Lugu Tri Handoko Handoko Lutviana N.D Marsoedi Marsoedi Marsoedi Marsoedi Maulana, Antajala Rian Mayor, Corazon Ell Merilia Papuani Milansari, Sindi Mimit Primyastanto Mimit Primyastanto Mimit Primyastanto Mohamad Fadjar Muh. Sulaiman Dadiono Muhammad Dailami, Muhammad Muhammad Fadjar Mujtahidah, Tholibah Nadia Dara Panggita Wati Nadiro, Vina Nur Niken Hendrakusma Wardani Nurcahyani, Shinta Eka Nurhalisa Nurhalisa Paricahya, Akhsan Fikrillah Pratama, Rakhmad Ndaru Putri, Radwa Fathin H R Adharyan Islamy Rahayu, Andra Rejekining Ramadhan, Surya Dewa Risnita Tri Utami Utami Rizal Akbar Hutagalung, Rizal Akbar Rohmah Widiastuti Rudianto Samsudin Hariyanto Saputra Fredo Seftiawan Samsu Rijal Septiana Sri Astuti, Septiana Sri Siahaan, Marthin Daniel Theo Gratia Sinaga, Sorbakti Soemarno Soemarno Soko Nuswantoro Sri Andayani Sri Andayani Sudjatmiko, Sudjatmiko Sufaichusan, Ifa Suharun Martudi Supriatna Tatang Tatang Taufik Budhi Pramono Taufik Budhi Pramono tholibah mujtahidah Tholibah Mujtahidah Veryl Hasan Vina Nur Nadiro Wahida Kartika Sari Wijaya, Rudy Yanuhar, Uun Yuni Kilawati Yunita Maimunah